Compositions isolated from forage grasses and methods for their use

ABSTRACT

Isolated polynucleotides encoding polypeptides active in the fructan, cellulose, starch and/or tannin biosynthetic pathways are provided, together with expression vectors and host cells comprising such isolated polynucleotides. Methods for the use of such polynucleotides and polypeptides are also provided.

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority to U.S. Provisional Patent Application 60/378,930, filed May 6, 2002, and U.S. Provisional Patent Application 60/408,782, filed Sep. 5, 2002.

TECHNICAL FIELD OF THE INVENTION

[0003] This invention relates to polynucleotides isolated from forage grass tissues, specifically from Lolium perenne (perennial ryegrass) and Festuca arundinacea (tall fescue), as well as oligonucleotide probes and primers, genetic constructs comprising the polynucleotides, biological materials (including host cells and plants) incorporating the polynucleotides, polypeptides encoded by the polynucleotides, and methods for using the polynucleotides and polypeptides. More particularly, the invention relates to polypeptides involved in the tannin, cellulose and fructan biosynthetic pathways, and to polynucleotides encoding such polypeptides.

BACKGROUND OF THE INVENTION

[0004] Over the past 50 years, there have been substantial improvements in the genetic production potential of ruminant animals (sheep, cattle and deer). Levels of meat, milk or fiber production that equal an animal's genetic potential may be attained within controlled feeding systems, where animals are fully fed with energy dense, conserved forages and grains. However, the majority of temperate farming systems worldwide rely on the in situ grazing of pastures. Nutritional constraints associated with temperate pastures can prevent the full expression of an animal's genetic potential. This is illustrated by a comparison between milk production by North American grain-fed dairy cows and New Zealand pasture-fed cattle. North American dairy cattle produce, on average, twice the milk volume of New Zealand cattle, yet the genetic base is similar within both systems (New Zealand Dairy Board and United States Department of Agriculture figures). Significant potential therefore exists to improve the efficiency of conversion of pasture nutrients to animal products through the correction of nutritional constraints associated with pastures.

[0005] Carbohydrate Metabolism

[0006] Plant carbohydrates can be divided into two groups depending on their function within the plant. Structural carbohydrates, such as cellulose and lignin, are usually part of the extracellular matrix. Non-structural, storage carbohydrates act as either long- or short-term carbohydrate stores. Examples of non-structural carbohydrates include starch, sucrose and fructans.

[0007] Fructans are polymers that are stored in the vacuole and that consist of linear and branched chains of fructose units (for review see Vijn and Smeekens Plant Physiol. 120:351-359, 199). They play an important role in assimilate partitioning and possibly in stress tolerance in many plant families. Grasses use fructans instead of starch as a water-soluble carbohydrate store (Pollock et al., in “Regulation of primary metabolic pathways in plants”, N. J. Kruger et al. (eds), Kluwer Academic Publishers, The Netherlands, pp 195-226, 1999). Increasing the amount of fructans and sucrose in forage crops leads to an increase in the level of water-soluble carbohydrates and thereby enhances the nutritional value of the plants. In addition, increasing the amount of fructans in forage plants decreases methane production in animals fed the plants, thereby leading to lower greenhouse gas emissions, and decreases urea production in animals as less protein is degraded in the rumen (Biggs and Hancock Trends in Plant Sci., 6:8-9, 2001). Fructans have also been implicated in protecting plants against water deficits caused by drought or low temperatures. Introduction of enzymes involved in the fructan biosynthetic pathway into plants that do not naturally synthesize fructans may be employed to confer cold tolerance and drought tolerance (Pilon-Smits, Plant Physiol. 107:125-130, 1995).

[0008] The number of fructose units within a fructan chain is referred to as the degree of polymerization (DP). In grasses, fructans of DP 6-10 are common. Such fructans of low DP are naturally sweet and are therefore of interest for use as sweeteners in foodstuffs. Long fructan chains form emulsions with a fat-like texture and a neutral taste. The human digestive system is unable to degrade fructans, and fructans of high DP may therefore be used as low-calorie food ingredients. Over-expression of enzymes involved in the fructan biosynthetic pathway may be usefully employed to produce quantities of fructans that can be purified for human consumption.

[0009] Five major classes of structurally different fructans have been identified in plants, with each class showing a different linkage of the fructosyl residues. Fructans found in grasses are of the mixed levan class, consisting of both (2-1)- and (2-6)-linked beta-D-fructosyl units (Pollock et al., in “Regulation of primary metabolic pathways in plants”, N. J. Kruger et al. (eds), Kluwer Academic Publishers, The Netherlands, pp195-226, 1999). Fructans are synthesized by the action of fructosyltransferase enzymes on sucrose in the vacuole. These enzymes are closely related to invertases, enzymes that normally hydrolyze sucrose.

[0010] Grasses use two fructosyltransferase enzymes to synthesize fructans, namely sucrose:sucrose 1-fructosyltransferase (1-SST) and sucrose:fructan 6-fructosyltransferase (6-SFT) (Pollock et al., in “Regulation of primary metabolic pathways in plants”, N. J. Kruger et al. (eds), Kluwer Academic Publishers, The Netherlands, pp195-226, 1999). 1-SST is a key enzyme in plant fructan biosynthesis, while 6-SFT catalyzes the formation and extension of beta-2,6-linked fructans that is typically found in grasses. Specifically, 1-SST catalyzes the formation of 1-kestose plus glucose from sucrose, while 6-SFT catalyzes the formation of bifurcose plus glucose from sucrose plus 1-kestose and also the formation of 6-kestose plus glucose from sucrose. Both enzymes can modify 1-kestose, 6-kestose and bifurcose further by adding additional fructose molecules. Over-expression of both 1-SST and 6-SFT in the same plant may be employed to produce fructans for use in human foodstuffs (Sevenier et al., Nature Biotechnology 16:843-846; Hellewege et al., Proc. Nat. Acad. Sci., U.S.A. 97:8699-8704). For a review of the fructan biosynthetic pathway see Vijn I. and Smeekens S. Plant Physiol. 120:351-359, 1999.

[0011] The synthesis of sucrose from photosynthetic assimilates in plants, and therefore the availability of sucrose for use in fructan formation, is controlled, in part, by the enzymes sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP). Sucrose plays an important role in plant growth and development, and is a major end product of photosynthesis. It also functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression (for review see Smeekens Curr. Opin. Plant Biol. 1:230-234, 1998). SPS regulates the synthesis of sucrose by regulating carbon partitioning in the leaves of plants and therefore plays a major role as a limiting factor in the export of photoassimilates out of the leaf. The activity of SPS is regulated by phosphorylation and moderated by concentration of metabolites and light (Huber et al., Plant Physiol. 95:291-297, 1991). Specifically, SPS catalyzes the transfer of glucose from UDP-glucose to fructose-6-phosphate, thereby forming sucrose-6-phosphate (Suc-6-P). Suc-6-P is then dephosphorylated by SPP to form sucrose (Lunn et al., Proc. Nat. Acad. Sci., U.S.A. 97:12914-12919, 2000). The enzymes SPS and SPP exist as a heterotetramer in the cytoplasm of mesophyll cells in leaves, with SPP functioning to regulate SPS activity. SPS is also important in ripening fruits, sprouting tubers and germinating seeds (Laporte et al. Planta 212:817-822, 2001).

[0012] Once in the vacuole, sucrose can be converted into fructan by fructosyltransferases as discussed above, or hydrolyzed into glucose and fructose by the hydrolase enzymes known as invertases (Sturm, Plant Physiol. 121:1-7, 1999). There are several different types of invertases, namely extracellular (cell wall), vacuolar (soluble acid) and cytoplasmic, with at least two isoforms of each type of invertase normally being found within a plant species. In addition to having different subcellular locations, the different types of invertases have different biochemical properties. For example, soluble and cell wall invertases operate at acidic pH, whereas cytoplasmic invertases work at a more neutral or alkaline pH. Invertases are believed to regulate the entry of sucrose into different utilization pathways (Grof and Campbell Aust. J. Plant Physiol. 28:1-12, 2001). Reduced invertase activity may increase the level of water-soluble carbohydrates in plants. Plants contain several isoforms of cell wall invertases (CWINV), which accumulate as soluble proteins. CWINV plays an important role in phloem unloading and in stress response. Arabidopsis contains 9 putative cytoplasmic or neutral invertases that are expressed in all tissues and at all developmental stages implying a more general function than the differentially expressed acid invertases. The neutral invertase cloned from carrot and Lolium temulentum show no similarity to acid invertases with the exception of a conserved pentapeptide motif in the grass cDNA (Gallagher J. Exp. Bot. 49:789, 1998; Sturm, A. et al., Physiologia Plantarum, 107:159-265, 1999).

[0013] Another enzyme that acts upon sucrose in plants is soluble sucrose synthase (SUS). Recent results indicate that SUS is localized in the cytosol, associated with the plasma membrane and the actin cytoskeleton. Phosphorylation of SUS is one of the factors controlling localization of the enzyme (Winter and Huber, Crit. Rev. Biochem. Mol. Biol. 35:253-89, 2000). It catalyzes the transfer of glucose from sucrose to UDP, yielding UDP-glucose and fructose. Increasing the amount of SUS in a plant increases the amount of cellulose synthesis, whereas decreasing SUS activity should increase fructan levels. Increased SUS concentration may also increase the yield of fruiting bodies. SUS activity is highest in carbon sink tissues in plants and low in photosynthetic source tissues, and studies have indicated that SUS is the main sucrose-cleaving activity in sink tissues. Grasses have two isoforms of SUS that are encoded by separate genes. These genes are differentially expressed in different tissues.

[0014] Pyrophosphate-fructose 6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) catalyses the reversible conversion of fructose 6-phosphate (Fru-6-P) and pyrophosphate (Pp_(i)) to fructose 1,6-bisphosphate (Fru-1,6-P) and inorganic phosphate (P_(i)). In the plant PFP has important physiological roles in glycosylation, sucrose metabolism, respiratory carbon flow, as well as being a supply of PP_(i). Along with FBPase and PFK, PFP regulates this step in the pathway of sucrose metabolism. PFP is a cytoplasmic enzyme consisting of a 250 kDa tetramer (two alpha and two beta chains) with the two subunits containing all of the regulatory and catalytical functions, respectively. In the plant cell fructose 2-6-bisphosphate is a potent activator of PFP activity. In sugarcane (a C₄ grass), PFP activity is inversely correlated with sucrose content (Whittaker and Botha Plant Physiol., 115, 1651-1659, 1997), indicating that a reduction of PFP enzyme levels will increase the flux of sucrose synthesis. In forage grasses reducing PFP levels in the leaves will increase water-soluble carbohydrate levels in the leaf tissue. The Arabidopsis genome contains four closely related PFP genes thought to encode two isoforms of each subunit, however, only 1 cDNA representing each unit of the purified protein has been isolated from Castor Bean, Potato and Spinach (Todd, Blakeley and Dennis Gene, 152, 181-186, 1995; Carlisle, Blakeley, Hemmingsen, Trevanion, Hiyoshi, Kruger and Dennis J. Biol. Chem., 265, 18366-18371, 1990).

[0015] Sucrose Transporters (SUTs) play a major role in the partitioning of dissacharides (sucrose) across membranes (for a review see Williams et al., Trends Plant Sci., 5:283-290, 2000). In particular SUTs are involved in loading and unloading of sucrose into the phloem and the source-sink relationship within the plant. SUTs are energy depenedent and can transport sucrose across large sucrose gradients. In Arabidopsis six SUTs have been identified, however in monocots and dicots SUTs form distinct groups. In general, monocots have 2 types of SUTs. For example barley and maize have two SUT proteins, known as SUT1 and SUT2. SUT1 is found in source, not sink, tissues, whereas SUT2 is constitutively expressed at similar levels in all tissues (Hirose, Imaizumi, Scofield, Furbank and Ohsugi Plant Cell Physiol. 38: 1389-1396; 1997; Weschke, et al., Plant Journal 21, 455-457, 2000). Inhibition of SUT1 in potato plants by antisense technology resulted in increased levels of sucrose and starch in the source leaves (Schulz et al. Planta, 206, 533-543, 1998). Repressing SUT activity in forage grasses to lower phloem loading in source tissues will increase water soluble carbohydrate content in the leaves.

[0016] Cellulose Synthesis

[0017] The major source of dietary fibre for grazing animals comes from plant cell walls. Mammals possess no enzymes capable for breaking down the polysaccharides in plant cell walls. Instead animals such as ruminants depend on microbial breakdown of plant cell walls through fermentation in either the rumen or large intestine.

[0018] Fibre in plants is measured using the Neutral Detergent Fibre (NDF) technique in which plant samples are boiled in a solution containing sodium lauryl sulfate (van Soest in “Nutritional Ecology of the Ruminant”. Cornell University Press, Ithaca, N.Y., 1994). This detergent extracts water-soluble components such as sugars, lipids and organic acids. The remaining insoluble residue (fibre) is termed NDF and consists predominantly of plant cell wall components such as cellulose, hemicellulose, and lignin. The amount of cellulose and lignin in cell walls can be determined using the Acid Detergent Fibre (ADF) method where plant samples are boiled in sulfuric acid and sodium lauryl sulfate. The difference between NDF and ADF for a plant sample is normally considered to be the amount of hemicellulose (van Soest in “Nutritional Ecology of the Ruminant”. Cornell University Press, Ithaca, N.Y., 1994).

[0019] Stems of most forage species have greater NDF content then leaves. For example, for a temperate C₃ grass in mid-flowering such as tall fescue (Festuca arundinacea), NDF content of leaves and stems is 50 and 70%, respectively (Buxton & Redfearn J. Nutrition 127:S814-S818, 1997). In contrast, for a C₄ tropical grass such as switchgrass (Panicum virgatum L.) the NDF content of leaves and stems is 70 and 85%, respectively. The digestibility of a forage is determined by cell wall content, so that legumes are more digestible than grasses because they contain less NDF. The NDF of a legume, however, is generally less digestible than that of grasses because a higher proportion of the NDF is made up by lignin. The increase of lignin as a component of NDF is also responsible for the decrease in digestibility of grasses at the time of flowering. In fact, ruminants can digest only 40-50% of NDF in legumes compared to 60-70% for grass NDF (Buxton & Redfearn J. Nutrition 127:S814-S818, 1997). Digestibility of cellulose by ruminants is therefore directly related to the extent of lignification. Generally hemicellulose is more digestible than cellulose.

[0020] Cellulose is the most abundant carbohydrate in forage making up to 20-40% of dry matter (van Soest in “Nutritional Ecology of the Ruminant”. Cornell University Press, Ithaca, N.Y., 1994). The cellulose in forages consists predominantly of β1-4 glucan (85%) and smaller amounts of pentosans (e.g. xylose and arabinose; 15%). There appear to be two pools of cellulose within the plant cell wall, the difference being one is lignified and the other is not (van Soest in “Nutritional Ecology of the Ruminant”. Cornell University Press, Ithaca, N.Y., 1994). The lignified cellulose is mostly found in the primary cell wall and in the S1 outer layer of the secondary cell wall. Independent of lignification, it appears that cellulose possesses a variability in nutritive quality (van Soest in “Nutritional Ecology of the Ruminant”. Cornell University Press, Ithaca, N.Y., 1994). This indicates that it is possible to alter the rate of cellulose digestibility by modifying the chemical composition of cellulose. This could be achieved through manipulating the actions of the cellulose synthesis and cellulose synthesis-like enzymes found in plant cells. One method to increase digestibility in this way is to increase the activity of the cellulose synthesis and cellulose synthesis-like enzymes responsible for synthesizing hemicellulose or to down regulate the cellulose synthesis and cellulose synthesis-like enzymes making cellulose. Hemicellulose is much more digestible than cellulose and is less likely to become lignified. Another way of manipulating cell wall composition is through modifying the rate and supply of primary components required for cellulose synthesis, i.e. of β1-4 glucan and pentosans such as xylose and arabinose. One way to achieve this is to modify the actions of soluble sucrose synthase and UDP glucose pyrophophorylase (UDP-GP) enzymes that produce the UDP-glucose required for cellulose synthesis. This may be further modified by manipulating the actions of the large and small subunits of ADP-glucose pyrophosphorylase (ADP-GP), the two enzymes that are rate-limiting steps in starch synthesis (Smith, Denyer and Martin Ann. Rev. Plant Phys. Plant Mol. Biol. 48:67-87, 1997).

[0021] Manipulating expression of the UDPGP and ADP-GP genes would alter the chemical composition of plant cell walls in forage plants. Altering cell wall biosynthesis therefore provides an opportunity to increase digestibility of the plant dry matter. This may be achieved by increasing the amount of carbon in the plant allocated to cellulose biosynthesis at the expense of lignin biosynthesis. Alternatively, decreasing the amount of cellulose biosynthesis and increasing the amount of water-soluble carbohydrates would have a similar effect. Furthermore, specifically increasing hemicellulose levels in the plant cell walls at expense of cellulose would also increase forage digestibility. By utilizing specific promoters in combination with the UDPGP and ADP-GP genes it is possible to increase or decrease the starch, cellulose and/or hemicellulose levels in the leaf or stem.

[0022] Tannin Biosynthetic Pathway

[0023] Condensed tannins are polymerized flavonoids. More specifically, tannins are composed of catechin 4-ol and catechin monomer units, and are stored in the vacuole. In many temperate forage crops, such as ryegrass and fescue, foliar tissues are tannin-negative. This leads to a high initial rate of fermentation when these crops are consumed by ruminant livestock resulting in both protein degradation and production of ammonia by the livestock. These effects can be reduced by the presence of low to moderate levels of tannin. In certain other plant species, the presence of high levels of tannins reduces palatability and nutritive value. Introduction of genes encoding enzymes involved in the biosynthesis of condensed tannins into a plant may be employed to synthesize flavonoid compounds that are not normally made in the plant. These compounds may then be isolated and used for treating human or animal disorders or as food additives.

[0024] Much of the biosynthetic pathway for condensed tannins is shared with that for anthocyanins, which are pigments responsible for flower color. Therefore, modulation of the levels of enzymes involved in the tannin biosynthetic pathway may be employed to alter the color of foliage and the pigments produced in flowers.

[0025] Most tannins described to date contain pro-cyanidin units derived from dihydroquercetin and pro-delphinidin units derived from dihydromyricetin. However, some tannins contain pro-pelargonidin units derived from dihydrokaempferol. The initial step in the tannin biosynthetic pathway is the condensation of coumaryl CoA with malonyl CoA to give naringenin-chalcone, which is catalyzed by the enzyme chalcone synthase (CHS). The enzyme chalcone isomerase (CHI) catalyzes the isomerization of naringenin chalcone to naringenin (also known as flavanone), which is then hydroxylated by the action of the enzyme flavonone 3-beta-hydroxylase (F3βH) to give dihydrokaempferol. The enzyme flavonoid 3′-hydroxylase (F3′OH) catalyzes the conversion of dihydrokaempferol to dihydroquercetin, which in turn can be converted into dihydromyricetin by the action of flavonoid 3′5′-hydroxylase (F3′5′OH). F3′OH is a P450 enzyme responsible for the brick red to orange pelargonidin-based pigments, whereas F3′5′OH is responsible for the purple and blue delphinidin-based pigments. The enzyme dihydroflavonol-4-reductase (DFR) catalyzes the last step before dihydrokaempferol, dihydroquercetin and dihydromyricetin are committed for either anthocyanin (flower pigment) or proanthocyanidin (condensed tannin) formation. DFR also converts dihydrokaempferol to afzelchin-4-ol, dihydroquercetin to catechin-4-ol, and dihydromyricetin to gallocatechin-4-ol, probably by the action of more than one isoform. For a review of the tannin biosynthetic pathway, see, Robbins M. P. and Morris P. in Metabolic Engineering of Plant Secondary Metabolism, Verpoorte and Alfermann (eds), Kluwer Academic Publishers, the Netherlands, 2000. The leucoanthocyanidin dioxygenase (LDOX) enzyme belongs to the iron/ascorbate-dependent family of oxidoreductases. In maize the LDOX gene A2 is required for the oxidation of leucoanthocyanidins into anthocyanidins (Menssen, Hoehmann, Martin, Schnable, Peterson, Saedler and Gierl EMBO J. 9:3051-3057, 1990).

[0026] While polynucleotides encoding some of the enzymes involved in the fructan, cellulose and tannin biosynthetic pathways have been isolated for certain species of plants, genes encoding many of the enzymes in a wide range of plant species have not yet been identified. Thus there remains a need in the art for materials useful in the modification of fructan and tannin content and composition in plants, and for methods for their use.

SUMMARY OF THE INVENTION

[0027] The present invention provides enzymes involved in the fructan, cellulose, starch and/or tannin biosynthetic pathways that are encoded by polynucleotides isolated from forage grass tissues. The polynucleotides were isolated from Lolium perenne (perennial ryegrass) and Festuca arundinacea (tall fescue) tissues taken at different times of the year, specifically in winter and spring, and from different parts of the plants, including: leaf blades, leaf base, pseudostems, roots and stems. Genetic constructs, expression vectors and host cells comprising the inventive polynucleotides are also provided, together with methods for using the inventive polynucleotides and genetic constructs to modulate the biosynthesis of fructans and tannins.

[0028] In specific embodiments, the isolated polynucleotides of the present invention comprise a sequence selected from the group consisting of: (a) SEQ ID NO: 1-44; (b) complements of SEQ ID NO: 1-44; (c) reverse complements of SEQ ID NO: 1-44; (d) reverse sequences of SEQ ID NO: 1-44; (e) sequences having a 99% probability of being functionally or evolutionarily related to a sequence of (a)-(d), determined as described below; and (f) sequences having at least 75%, 80%, 90%, 95% or 98% identity to a sequence of (a)-(d), the percentage identity being determined as described below. Polynucleotides comprising at least a specified number of contiguous residues (“x-mers”) of any of SEQ ID NO: 1-44, and oligonucleotide probes and primers corresponding to SEQ ID NO: 1-44 are also provided. All of the above polynucleotides are referred to herein as “polynucleotides of the present invention.”

[0029] In further aspects, the present invention provides isolated polypeptides encoded by the inventive polynucleotides. In specific embodiments, such polypeptides comprise an amino acid sequence of SEQ ID NO: 45-88. The present invention also provides polypeptides comprising a sequence having at least 75%, 80%, 90%, 95% or 98% identity to a sequence of SEQ ID NO: 45-88, wherein the polypeptide possesses the same functional activity as the polypeptide comprising a sequence of SEQ ID NO: 45-88. The present invention also contemplates isolated polypeptides comprising at least a functional portion of an amino acid sequence selected from the group consisting of: (a) SEQ ID NO: 45-88; and (b) sequences having at least 75%, 80%, 90%, 95% or 98% identity to a sequence of SEQ ID NO: 45-88.

[0030] In another aspect, the present invention provides genetic constructs, or expression vectors, comprising a polynucleotide of the present invention, either alone, in combination with one or more of the inventive sequences, or in combination with one or more known polynucleotides.

[0031] In certain embodiments, the present invention provides genetic constructs comprising, in the 5′-3′ direction: a gene promoter sequence; an open reading frame coding for at least a functional portion of a polypeptide of the present invention; and a gene termination sequence. An open reading frame may be orientated in either a sense or anti-sense direction. Genetic constructs comprising a non-coding region of a polynucleotide of the present invention or a polynucleotide complementary to a non-coding region, together with a gene promoter sequence and a gene termination sequence, are also provided. Preferably, the gene promoter and termination sequences are functional in a host cell, such as a plant cell. Most preferably, the gene promoter and termination sequences are those of the original enzyme genes but others generally used in the art, such as the Cauliflower Mosaic Virus (CMV) promoter, with or without enhancers, such as the Kozak sequence or Omega enhancer, and the Agrobacterium tumefaciens nopalin synthase terminator may be usefully employed in the present invention. Tissue-specific promoters may be employed in order to target expression to one or more desired tissues. The construct may further include a marker for the identification of transformed cells.

[0032] In a further aspect, transgenic cells, such as transgenic plant cells, comprising the genetic constructs of the present invention are provided, together with tissues and plants comprising such transgenic cells, and fruits, seeds and other products, derivatives, or progeny of such plants.

[0033] In yet another aspect, the present invention provides methods for modulating the fructan, cellulose, starch and/or tannin content and composition of a target organism, such as a plant, by modulating the amount and/or activity of an inventive polynucleotide or polypeptide in the organism. In certain embodiments, such methods include stably incorporating into the genome of the target plant a genetic construct of the present invention. In a preferred embodiment, the target plant is a forage grass, preferably selected from the group consisting of Lolium and Festuca species, and most preferably from the group consisting of Lolium perenne and Festuca arundinacea.

[0034] In a related aspect, methods for producing a plant having altered fructan or tannin composition is provided. Such methods comprise modulating the amount and/or activity of an inventive polynucleotide or polypeptide in a plant cell by, for example, transforming a plant cell with a genetic construct of the present invention to provide a transgenic cell, and cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.

[0035] In yet a further aspect, the present invention provides methods for modifying the activity of an enzyme in a target organism, such as a plant, comprising modulating the amount and/or activity of an inventive polynucleotide or polypeptide in the target organism by, for example stably incorporating into the genome of the target organism a genetic construct of the present invention. In a preferred embodiment, the target plant is a forage grass, preferably selected from the group consisting of Lolium and Festuca species, and most preferably from the group consisting of Lolium perenne and Festuca arundinacea.

BRIEF DESCRIPTION OF THE FIGURES

[0036]FIG. 1 shows the neutral invertase activity of the recombinant grass alkaline/neutral invertase protein AN_INV8 from L. perenne (amino acid sequence provided in SEQ ID NO: 56; cDNA sequence provided in SEQ ID NO: 12). Activity was measured as the μg of glucose release from cleavage of sucrose per hour at pH 7. Also shown is an empty vector negative control (pET41a).

[0037]FIG. 2 shows the PFP activity of L. perenne and F. arundinacea PFPA and PFPB subunits in coupled reactions. Amino acid sequences for L. perenne PFPA and PFPB are given in SEQ ID NO: 59 and 62, respectively (corresponding cDNA sequences are SEQ ID NO: 15 and 18), and amino acid sequences for F. arundinacea PFPA and PFPB are given in SEQ ID NO: 60 and 63, respectively (corresponding cDNA sequences are SEQ ID NO: 16 and 19). Oxidation of NADH was measured as nmoles PPi converted.

[0038]FIG. 3 shows the amino acid sequence of SEQ ID NO: 45. The conserved UTP-glucose-1-phosphate uridylyltransferase domain is underlined.

[0039]FIG. 4 shows the amino acid sequence of SEQ ID NO: 46. The conserved UTP-glucose-1-phosphate uridylyltransferase domain is underlined.

[0040]FIG. 5 shows the amino acid sequence of SEQ ID NO: 47. The conserved glycoside hydrolase, family 32 domain is underlined.

[0041]FIG. 6 shows the amino acid sequence of SEQ ID NO: 48. A transmembrane domain is underlined.

[0042]FIG. 7 shows the amino acid sequence of SEQ ID NO: 53. The signal peptide is in bold/italics.

[0043]FIG. 8 shows the amino acid sequence of SEQ ID NO: 54. The signal peptide is in bold/italics and two conserved Antifreeze protein, type I domains are underlined.

[0044]FIG. 9 shows the amino acid sequence of SEQ ID NO: 55. The signal peptide is in bold/italics.

[0045]FIG. 10 shows the amino acid sequence of SEQ ID NO: 56. Two transmembrane domains are double underlined.

[0046]FIG. 11 shows the amino acid sequence of SEQ ID NO: 57. Two transmembrane domains are double underlined.

[0047]FIG. 12 shows the amino acid sequence of SEQ ID NO: 58. Two transmembrane domains are double underlined.

[0048]FIG. 13 shows the amino acid sequence of SEQ ID NO: 59. The conserved phosphofructokinase domain is underlined and a transmembrane domain is double underlined.

[0049]FIG. 14 shows the amino acid sequence of SEQ ID NO: 60. The conserved phosphofructokinase domain is underlined and a transmembrane domain is double underlined.

[0050]FIG. 15 shows the amino acid sequence of SEQ ID NO: 61. The conserved phosphofructokinase is underlined.

[0051]FIG. 16 shows the amino acid sequence of SEQ ID NO: 62. The conserved phosphofructokinase domain is underlined.

[0052]FIG. 17 shows the amino acid sequence of SEQ ID NO: 63. The conserved phosphofructokinase domain is underlined.

[0053]FIG. 18 shows the amino acid sequence of SEQ ID NO: 64. The conserved glycosyl transferase, group 1 domain is underlined and two transmembrane domains are double underlined.

[0054]FIG. 19 shows the amino acid sequence of SEQ ID NO: 65. The conserved glycosyl transferase, group 1 domain is underlined and two transmembrane domains are double underlined.

[0055]FIG. 20 shows the amino acid sequence of SEQ ID NO: 66. The conserved substrate transporter domain is in bold and eleven transmembrane domains are double underlined.

[0056]FIG. 21 shows the amino acid sequence of SEQ ID NO: 67. Nine transmembrane domains are double underlined.

[0057]FIG. 22 shows the amino acid sequence of SEQ ID NO: 68. The conserved substrate transporter domain is in bold and eleven transmembrane domains are double underlined.

[0058]FIG. 23 shows the amino acid sequence of SEQ ID NO: 69. The conserved substrate transporter domain is in bold and eleven transmembrane domains are double underlined.

[0059]FIG. 24 shows the amino acid sequence of SEQ ID NO: 70. The conserved substrate transporter domain is in bold and eleven transmembrane domains are double underlined.

[0060]FIG. 25 shows the amino acid sequence of SEQ ID NO: 72. The conserved nucleotidyl transferase domain is in bold and three ADP-glucose pyrophosphorylase are boxed. Nine transmembrane domains are double underlined.

[0061]FIG. 26 shows the amino acid sequence of SEQ ID NO: 73. The conserved nucleotidyl transferase domain is in bold and three ADP-glucose pyrophosphorylase domains are boxed. A transmembrane domain is double underlined.

[0062]FIG. 27 shows the amino acid sequence of SEQ ID NO: 74. The conserved nucleotidyl transferase domain is in bold and three ADP-glucose pyrophosphorylase domains are boxed. A transmembrane domain is double underlined.

[0063]FIG. 28 shows the amino acid sequence of SEQ ID NO: 75. The conserved nucleotidyl transferase domain is in bold and three ADP-glucose pyrophosphorylase domains are boxed. The signal peptide is in bold/italics and a transmembrane domain is double underlined.

[0064]FIG. 29 shows the amino acid sequence of SEQ ID NO: 76. The conserved naringenin-chalcone synthase domain is underlined. The signal peptide is in bold/italics and a transmembrane domain is double underlined.

[0065]FIG. 30 shows the amino acid sequence of SEQ ID NO: 77. The conserved naringenin-chalcone synthase domain is underlined and two transmembrane domains are double underlined.

[0066]FIG. 31 shows the amino acid sequence of SEQ ID NO: 78. The conserved naringenin-chalcone synthase domain is underlined and two transmembrane domains are double underlined.

[0067]FIG. 32 shows the amino acid sequence of SEQ ID NO: 79. A transmembrane domain is double underlined.

[0068]FIG. 33 shows the amino acid sequence of SEQ ID NO: 80. A transmembrane domain is double underlined.

[0069]FIG. 34 shows the amino acid sequence of SEQ ID NO: 81. A transmembrane domain is double underlined.

[0070]FIG. 35 shows the amino acid sequence of SEQ ID NO: 82. The conserved Cytochrome P450 domain is underlined and three transmembrane domains are double underlined.

[0071]FIG. 36 shows the amino acid sequence of SEQ ID NO: 83. The conserved Cytochrome P450 domain is boxed, the signal peptide is in bold and a transmembrane domain is double underlined.

[0072]FIG. 37 shows the amino acid sequence of SEQ ID NO: 84. The conserved Cytochrome P450 domain is boxed and three transmembrane domains are double underlined.

[0073]FIG. 38 shows the amino acid sequence of SEQ ID NO: 85. The conserved Cytochrome P450 domain is boxed, the signal peptide is in bold/italics and three transmembrane domains are double underlined.

[0074]FIG. 39 shows the amino acid sequence of SEQ ID NO: 86. The conserved Cytochrome P450 domain is boxed and three transmembrane domains are double underlined.

[0075]FIG. 40 shows the amino acid sequence of SEQ ID NO: 87. The conserved Cytochrome P450 domain is boxed, the signal peptide is in bold/italics and three transmembrane domains are double underlined.

[0076]FIG. 41 shows the amino acid sequence of SEQ ID NO: 88. The conserved 20G-Fe(II) oxygenase superfamily domain is underlined.

DETAILED DESCRIPTION OF THE INVENTION

[0077] The polypeptides of the present invention, and the polynucleotides encoding such polypeptides, have activity in fructan, cellulose, starch and/or tannin biosynthetic pathways in plants. Using the methods and materials of the present invention, the fructan, cellulose, starch and/or tannin content of a plant may be modulated by modulating expression of polynucleotides of the present invention, or by modifying the activity of the polynucleotides or polypeptides encoded by the polynucleotides. The isolated polynucleotides and polypeptides of the present invention may thus be usefully employed in the correction of nutritional imbalances associated with temperate pastures and to increase the yield of animal products from pastures.

[0078] The fructan, cellulose, starch and/or tannin content of a target organism, such as a plant, may be modified, for example, by incorporating additional copies of genes encoding enzymes involved in the fructan, cellulose, starch and/or tannin biosynthetic pathways into the genome of the target plant. Similarly, a modified fructan, cellulose, starch and/or tannin content can be obtained by transforming the target plant with anti-sense copies of such genes. In addition, the number of copies of genes encoding for different enzymes in the fructan, cellulose, starch and tannin biosynthetic pathways can be manipulated to modify the relative amount of each monomer unit synthesized, thereby leading to the formation of fructans, cellulose, starch or tannins having altered composition.

[0079] The present invention thus provides methods for modulating the polynucleotide and/or polypeptide content and composition of an organism. In certain embodiments, such methods involve stably incorporating into the genome of the organism a genetic construct comprising one or more polynucleotides of the present invention. In one embodiment, the target organism is a plant species, preferably a forage plant, more preferably a grass of the Lolium or Festuca species, and most preferably Lolium perenne or Festuca arundinacea. In related aspects, methods for producing a plant having an altered genotype or phenotype is provided, such methods comprising transforming a plant cell with a genetic construct of the present invention to provide a transgenic cell, and cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth. Plants having an altered genotype or phenotype as a consequence of modulation of the level or content of a polynucleotide or polypeptide of the present invention compared to a wild-type organism, as well as components (seeds, etc.) of such plants, and the progeny of such plants, are contemplated by and encompassed within the. present invention.

[0080] The isolated polynucleotides of the present invention additionally have utility in genome mapping, in physical mapping, and in positional cloning of genes. The polynucleotide sequences identified as SEQ ID NOS: 1-44 and their variants, may also be used to design oligonucleotide probes and primers. Oligonucleotide probes and primers have sequences that are substantially complementary to the polynucleotide of interest over a certain portion of the polynucleotide, preferably over substantially the entire length of the polynucleotides. Oligonucleotide probes designed using the inventive polynucleotides may be employed to detect the presence and examine the expression patterns of genes in any organism having sufficiently similar DNA and RNA sequences in their cells using techniques that are well known in the art, such as slot blot DNA hybridization techniques. Oligonucleotide primers designed using the polynucleotides of the present invention may be used for PCR amplifications. Oligonucleotide probes and primers designed using the inventive polynucleotides may also be used in connection with various microarray technologies, including the microarray technology of Affymetrix (Santa Clara, Calif.).

[0081] In a first aspect, the present invention provides isolated polynucleotide sequences identified in the attached Sequence Listing as SEQ ID NOS: 1-44, and polypeptide sequences identified in the attached Sequence Listing as SEQ ID NO: 45-88. The polynucleotides and polypeptides of the present invention have demonstrated similarity to the following polypeptides that are known to be involved in fructan, cellulose, starch and/or tannin biosynthetic processes: TABLE 1 SEQ ID NO: SEQ ID NO: DNA polypeptide Category Description 1, 2 45, 46 Carbohydrate Homolog of UDP-glucose pyrophosphorylase metabolism (EC 2.7.7.9) (UDPGP or UGPASE) which is one of the key enzymes of the carbohydrate metabolic pathway. It plays a central role as glucosyl donor in cellular metabolic pathways. UDP-glucose pyrophosphorylase catalyzes the reversible uridylyl transfer from UDP-glucose to MgPPi, forming glucose 1-phosphate and MgUTP. 3, 4 47, 48 Fructan Homolog of Sucrose (Suc): Suc 1-fructosyl- metabolism transferase (1-SST) isolated from L. perenne. 1-SST is the key enzyme in plant fructan biosynthesis and catalyzes the de novo fluctan synthesis from sucrose. Fructans play an important role in assimilation partitioning and in stress tolerance in many plants. It contains a typical signature of the glycosyl hydrolases family 32 (amino acid residues 126 to 139). The glycosyl hydrolases family 32 domain signature has a consensus of HYQPxxH/NxxNDPNG, where D is the active site residue (Henrissat, Biochem. J. 280: 309-316, 1991).  5-14 49-58 Fructan Homolog of alkaline/neutral invertase (AN- metabolism INV) that is involved in catalyzing sucrose into hexoses for utilization as a source of carbon and energy. AN-INV belongs to the family 32 of glycosyl hydrolases. Neutral invertase is an octamer of 456 kDa with subunits of 57 kDa, whereas alkaline invertase is a 504 kDa tetramer with subunits of 126 kDa. Neutral invertase also hydrolyzes raffinose and stachyose and, therefore, is a beta-fructo- furanosidase. In contrast, alkaline invertase is highly specific for sucrose (Lee and Sturm, Plant Physiol. 112: 1513-1522, 1996). 15, 16 59, 60 Fructan Homologue of the alpha subunit of Pyro- metabolism phosphate-dependent 6-phosphofructo-1- phosphotransferase (PFPA) that plays a role in carbohydrate metabolism. PFP is involved in the first step of glycolysis in the phosphorylation of fructose 6-phosphate (Fru 6- P). PFPA acts as a regulatory protein in regulating both the catalytic activity and the Fru-2,6-P2-binding affinity of the beta subunit (Siebers et al., J. Bacteriol. 180: 2137-2143, 1998). 17-19 61-63 Fructan Homolog of the beta subunit of Pyrophosphate- metabolism dependent 6-phosphofructo-1-phospho- transferase (PFPB) which plays a role in carbohydrate metabolism. PFP is involved in the first step of glycolysis in the phosphorylation of fructose 6-phosphate (Fru-6- P). The catalytic activity of the PFP enzyme is associated with the beta subunit PFPB while PFPA acts as a regulatory protein in regulating both the catalytic activity and the Fru-2,6-P2- binding affinity of the beta subunit (Carlisle et al., J. Biol. Chem. 265: 18366-18371, 1990; Siebers et al., J. Bacteriol. 180: 2137-2143, 1998). 20, 21 64, 65 Fructan Homologue of sucrose phosphate synthase metabolism which is involved in the sucrose synthesis pathway. Sucrose plays an important role in plant growth and development and is a major end product of photosynthesis. It also functions as a primary transport sugar and in some cases as a direct or indirect regulator of gene expression. SPS-1 regulates the synthesis of sucrose by regulating carbon partitioning in the leaves of plants and therefore plays a major role as a limiting factor in the export of photoassimilates out of the leaf. The activity of SPS is regulated by phosphorylation and moderated by concentration of metabolites and light. 22-24 66-68 Fructan Homologue of the sugar transporter SUT1, a metabolism member of the SUT family of low- and high- affinity sucrose transporters that is involved in transport of sucrose from mature leaves via the phloem. Expression of SUT1 has also been observed in other tissues (stems and parts of flower) suggesting that SUT1 may also have other functions, such as sucrose retrieval and phloem unloading (Burkle et al., Plant Physiol. 118: 59-68, 1998). 25, 26 69, 70 Fructan Homologue of sugar transporter SUT2, a metabolism member of the SUT family of low- and high- affinity sucrose transporters. SUT2 is more highly expressed in sink than in source leaves, is inducible by sucrose and regulates the relative activity of low- and high-affinity sucrose transport into sieve elements (Barker et al., Plant Cell 12: 1153-1164, 2000). 27 71 Fructan Homologue of a sugar transporter, a member of metabolism the SUT family of low- and high-affinity sucrose transporters that is involved in transport of sucrose from mature leaves via the phloem. 28, 29 72, 73 Fructan Homolog of the large subunit (LSU) of ADP- metabolism glucose pyrophosphorylase (AGPase), which plays a role in starch biosynthesis. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from glucose-1-phosphate and ATP. The enzyme is found in chloroplasts of leaves and amyloplasts of developing endosperm. AGPase belongs to the glucose-1- phosphate adenylyltransferase family. 30, 31 74, 75 Carbohydrate Homolog of the small subunit (SSU) of ADP- metabolism glucose pyrophosphorylase (AGPase), which plays a role in starch biosynthesis. It catalyzes the synthesis of the activated glycosyl donor, ADP-glucose from glucose-1-phosphate and ATP. The enzyme is found in chloroplasts of leaves and amyloplasts of developing endosperm. AGPase belongs to the glucose-1- phosphate adenylyltransferase family. 32, 33 76, 77 Tannin Homolog of Chalcone Synthase (CHS) which biosynthesis is an important enzyme in flavonoid synthesis. The molecule contains a conserved chalcone synthase active site (Lanz et al., J. Biol. Chem. 266: 9971-9976, 1991) at amino acid residues 166 to 176, with the conserved Cys residue at position 167. 34-37 78-81 Tannin Homologue of dihydroflavonal-4-reductase metabolism (DFR) that belongs to the dihydroflavonol-4- reductases family and is involved in the flavonoid synthesis and anthocyanidins biosynthesis. Flavonoids are secondary metabolites derived from phenylalanine and acetate metabolism that perform a variety of essential functions in higher plants. 38-43 82-87 Tannin Homologue of flavonoid 3′-hydroxylase (F3′H) metabolism which is a key enzyme in the flavonoid pathway leading to the production of the colored anthocyanins where it is involved in determination of flower coloring. Anthocyanins synthesized in plants are controlled by flavonoid 3′-hydroxylase and flavonoid 3′,5′- hydroxylase which are members of the cytochrome P450 family, a large group of membrane-bound heme-containing enzymes that are involved in a range of NADPH- and O2-dependent hydroxylation reactions. Plants have evolved a large number of different P450 enzymes for the synthesis of secondary metabolites. The F3 ′H transcript is most abundant in petals from flowers at an early stage of development and levels decline as the flower matures. Transcripts are also detected in the ovaries, sepals, peduncles, stems and anthers of the petunia plant (Brugliera et al., Plant J. 19: 441-451, 1999 44 88 Tannin Homologue of leucoanthocyanidin dioxygenase biosynthesis (LDOX) which is an enzyme in the flavonoid biosynthesis pathway. LDOX is expressed as a late gene in the flavonoid biosynthesis pathway.

[0082] All the polynucleotides and polypeptides provided by the present invention are isolated and purified, as those terms are commonly used in the art. Preferably, the polypeptides and polynucleotides are at least about 80% pure, more preferably at least about 90% pure, and most preferably at least about 99% pure.

[0083] The word “polynucleotide(s),” as used herein, means a polymeric collection of nucleotides, and includes DNA and corresponding RNA molecules and both single and double stranded molecules, including HnRNA and mRNA molecules, sense and anti-sense strands of DNA and RNA molecules, and comprehends cDNA, genomic DNA, and wholly or partially synthesized polynucleotides. A polynucleotide of the present invention may be an entire gene, or any portion thereof. As used herein, a “gene” is a DNA sequence which codes for a functional protein or RNA molecule. Operable anti-sense polynucleotides may comprise a fragment of the corresponding polynucleotide, and the definition of “polynucleotide” therefore includes all operable anti-sense fragments. Anti-sense polynucleotides and techniques involving anti-sense polynucleotides are well known in the art and are described, for example, in Robinson-Benion et al., Methods in Enzyol. 254(23): 363-375, 1995 and Kawasaki et al., Artific. Organs 20(8): 836-848, 1996.

[0084] In specific embodiments, the present invention provides isolated polynucleotides comprising a sequence of SEQ ID NO: 1-44; polynucleotides comprising variants of SEQ ID NO: 1-44; polynucleotides comprising extended sequences of SEQ ID NO: 1-44 and their variants, oligonucleotide primers and probes corresponding to the sequences set out in SEQ ID NO: 1-44 and their variants, polynucleotides comprising at least a specified number of contiguous residues of any of SEQ ID NO: 1-44 (x-mers), and polynucleotides comprising extended sequences which include portions of the sequences set out in SEQ ID NO: 1-44, all of which are referred to herein, collectively, as “polynucleotides of the present invention.” Polynucleotides that comprise complements of such polynucleotide sequences, reverse complements of such polynucleotide sequences, or reverse sequences of such polynucleotide sequences, together with variants of such sequences, are also provided.

[0085] The definition of the terms “complement(s),” “reverse complement(s),” and “reverse sequence(s),” as used herein, is best illustrated by the following example. For the sequence 5′ AGGACC 3′, the complement, reverse complement, and reverse sequence are as follows: complement 3′ TCCTGG 5′ reverse complement 3′ GGTCCT 5′ reverse sequence. 5′ CCAGGA 3′

[0086] Preferably, sequences that are complements of a specifically recited polynucleotide sequence are complementary over the entire length of the specific polynucleotide sequence.

[0087] As used herein, the term “x-mer,” with reference to a specific value of “x,” refers to a polynucleotide comprising at least a specified number (“x”) of contiguous residues of: any of the polynucleotides provided in SEQ ID NO: 1-44. The value of x may be from about 20 to about 600, depending upon the specific sequence.

[0088] Polynucleotides of the present invention comprehend polynucleotides comprising at least a specified number of contiguous residues (x-mers) of any of the polynucleotides identified as SEQ ID NO: 1-44, or their variants. Similarly, polypeptides of the present invention comprehend polypeptides comprising at least a specified number of contiguous residues (x-mers) of any of the polypeptides identified as SEQ ID NO: 45-88. According to preferred embodiments, the value of x is at least 20, more preferably at least 40, more preferably yet at least 60, and most preferably at least 80. Thus, polynucleotides of the present invention include polynucleotides comprising a 20-mer, a 40-mer, a 60-mer, an 80-mer, a 100-mer, a 120-mer, a 150-mer, a 180-mer, a 220-mer, a 250-mer; or a 300-mer, 400-mer, 500-mer or 600-mer of a polynucleotide provided in SEQ ID NO: 1-44, or a variant of one of the polynucleotides corresponding to the polynucleotides provided in SEQ ID NO: 1-44. Polypeptides of the present invention include polypeptides comprising a 20-mer, a 40-mer, a 60-mer, an 80-mer, a 100-mer, a 120-mer, a 150-mer, a 180-mer, a 220-mer, a 250-mer; or a 300-mer, 400-mer, 500-mer or 600-mer of a polypeptide provided in SEQ ID NO: 45-88, or a variant thereof.

[0089] The polynucleotides of the present invention were isolated by high throughput sequencing of cDNA libraries prepared from forage grass tissue collected from Lolium perenne and Festuca arundinacea. Some of the polynucleotides of the present invention may be “partial” sequences, in that they do not represent a full-length gene encoding a full-length polypeptide. Such partial sequences may be extended by analyzing and sequencing various DNA libraries using primers and/or probes and well known hybridization and/or PCR techniques. Partial sequences may be extended until an open reading frame encoding a polypeptide, a full-length polynucleotide and/or gene capable of expressing a polypeptide, or another useful portion of the genome is identified. Such extended sequences, including full-length polynucleotides and genes, are described as “corresponding to” a sequence identified as one of the sequences of SEQ ID NO: 1-44 or a variant thereof, or a portion of one of the sequences of SEQ ID NO: 1-44 or a variant thereof, when the extended polynucleotide comprises an identified sequence or its variant, or an identified contiguous portion (x-mer) of one of the sequences of SEQ ID NO: 1-44 or a variant thereof. Similarly, RNA sequences, reverse sequences, complementary sequences, anti-sense sequences and the like, corresponding to the polynucleotides of the present invention, may be routinely ascertained and obtained using the cDNA sequences identified as SEQ ID NO: 1-44.

[0090] The polynucleotides identified as SEQ ID NOS: 1-44 contain open reading frames (“ORFs”) encoding polypeptides and functional portions of polypeptides. Open reading frames may techniques that are well known in the art. These techniques include, for the location of known start and stop codons, most likely reading frame identification based on codon frequencies, etc. Suitable tools and software for ORF analysis are well known in the art and include, for example, GeneWise, available from The Sanger Center, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom; Diogenes, available from Computational Biology Centers, University of Minnesota, Academy Health Center, UMHG Box 43 Minneapolis Minn. 55455; and GRAIL, available from the Informatics Group, Oak Ridge National Laboratories, Oak Ridge, Tennessee Tenn. Once a partial open reading frame is identified, the polynucleotide may be extended in the area of the partial open reading frame using techniques that are well known in the art until the polynucleotide for the full open reading frame is identified.

[0091] The location of ORFs (by nucleotide position) contained within SEQ ID NO: 1-44, and the corresponding amino acid sequences are provided in Table 2 below. TABLE 2 SEQ ID NO: SEQ ID NO: Polynucleotide ORF Polypeptide 1  72-1493 45 2  66-1481 46 3  0-1607 47 4  1-1914 48 5 123-1934 49 6  0-1671 50 7 114-1979 51 8  0-737 52 9  47-1783 53 10 170-2029 54 11 113-1849 55 12 154-1818 56 13 211-1866 57 14  79-1767 58 15  76-1926 59 16  80-1930 60 17  91-1782 61 18  91-1782 62 19  84-1775 63 20  97-2994 64 21 112-3065 65 22 226-1794 66 23  0-1226 67 24 243-1811 68 25 207-1727 69 26 101-1615 70 27 108-1634 71 28 150-1718 72 29 169-1737 73 30  12-1589 74 31  5-1579 75 32 136-1332 76 33 136-1332 77 34 95-836 78 35  95-1123 79 36 82-847 80 37  82-1104 81 38  0-1532 82 39  58-1632 83 40  0-1580 84 41  16-1596 85 42  0-1478 86 43  20-1519 87 44 117-1259 88

[0092] Once open reading frames are identified, the open reading frames may be isolated and/or synthesized. Expressible genetic constructs comprising the open reading frames and suitable promoters, initiators, terminators, etc., which are well known in the art, may then be constructed. Such genetic constructs may be introduced into a host cell to express the polypeptide encoded by the open reading frame. Suitable host cells may include various prokaryotic and eukaryotic cells, including plant cells, mammalian cells, bacterial cells, algae and the like.

[0093] The polynucleotides of the present invention may be isolated by high throughput sequencing of cDNA libraries prepared from forage grass tissue, as described below in Example 1. Alternatively, oligonucleotide probes and primers based on the sequences provided in SEQ ID NO: 1-44 can be synthesized as detailed below, and used to identify positive clones in either cDNA or genomic DNA libraries from forage grass tissue cells by means of hybridization or polymerase chain reaction (PCR) techniques. Hybridization and PCR techniques suitable for use with such oligonucleotide probes are well known in the art (see, for example, Mullis et al., Cold Spring Harbor Symp. Quant. Biol., 51:263, 1987; Erlich, ed., PCR technology, Stockton Press: NY, 1989; and Sambrook et al., eds., Molecular cloning: a laboratory manual, 2nd ed., CSHL Press: Cold Spring Harbor, N.Y., 1989). In addition to DNA-DNA hybridization, DNA-RNA or RNA-RNA hybridization assays are also possible. In the first case, the MRNA from expressed genes would then be detected instead of genomic DNA or cDNA derived from MRNA of the sample. In the second case, RNA probes could be used. Artificial analogs of DNA hybridizing specifically to target sequences could also be employed. Positive clones may be analyzed by restriction enzyme digestion, DNA sequencing or the like.

[0094] The polynucleotides of the present invention may also, or alternatively, be synthesized using techniques that are well known in the art. The polynucleotides may be synthesized, for example, using automated oligonucleotide synthesizers (e.g., Beckman Oligo 1000M DNA Synthesizer; Beckman Coulter Ltd., Fullerton, Calif.) to obtain polynucleotide segments of up to 50 or more nucleic acids. A plurality of such polynucleotide segments may then be ligated using standard DNA manipulation techniques that are well known in the art of molecular biology. One conventional and exemplary polynucleotide synthesis technique involves synthesis of a single stranded polynucleotide segment having, for example, 80 nucleic acids, and hybridizing that segment to a synthesized complementary 85 nucleic acid segment to produce a 5 nucleotide overhang. The next segment may then be synthesized in a similar fashion, with a 5 nucleotide overhang on the opposite strand. The “sticky” ends ensure proper ligation when the two portions are hybridized. In this way, a complete polynucleotide of the present invention may be synthesized entirely in vitro.

[0095] Oligonucleotide probes and primers complementary to and/or corresponding to SEQ ID NO: 1-44 and variants of those sequences, are also comprehended by the present invention. Such oligonucleotide probes and primers are substantially complementary to the polynucleotide of interest over a certain portion of the polynucleotide. An oligonucleotide probe or primer is described as “corresponding to” a polynucleotide of the present invention, including one of the sequences set out as SEQ ID NO: 1-44 or a variant thereof, if the oligonucleotide probe or primer, or its complement, is contained within one of the sequences set out as SEQ ID NOS: 1-44 or a variant of one of the specified sequences.

[0096] Two single stranded sequences are said to be substantially complementary when the nucleotides of one strand, optimally aligned and compared, with the appropriate nucleotide insertions and/or deletions, pair with at least 80%, preferably at least 90% to 95%, and more preferably at least 98% to 100%, of the nucleotides of the other strand. Alternatively, substantial complementarity exists when a first DNA strand will selectively hybridize to a second DNA strand under stringent hybridization conditions.

[0097] In specific embodiments, the inventive oligonucleotide probes and/or primers comprise at least about 6 contiguous residues, more preferably at least about 10 contiguous residues, and most preferably at least about 20 contiguous residues complementary to a polynucleotide sequence of the present invention. Probes and primers of the present invention may be from about 8 to 100 base pairs in length, preferably from about 10 to 50 base pairs in length, and more preferably from about 15 to 40 base pairs in length. The probes can be easily selected using procedures well known in the art, taking into account DNA-DNA hybridization stringencies, annealing and melting temperatures, potential for formation of loops, and other factors which are well known in the art. Preferred techniques for designing PCR primers are disclosed in Dieffenbach and Dyksler, PCR Primer: a laboratory manual, CSHL Press: Cold Spring Harbor, N.Y., 1995. A software program suitable for designing probes, and especially for designing PCR primers, is available from Premier Biosoft International, 3786 Corina Way, Palo Alto, Calif. 94303-4504.

[0098] The isolated polynucleotides of the present invention also have utility in genome mapping, in physical mapping, and in positional cloning of genes.

[0099] The polynucleotides identified as SEQ ID NO: 1-44 were isolated from cDNA clones and represent sequences that are expressed in the tissue from which the cDNA was prepared. RNA sequences, reverse sequences, complementary sequences, anti-sense sequences, and the like, corresponding to the polynucleotides of the present invention, may be routinely ascertained and obtained using the cDNA sequences identified as SEQ ID NO: 1-44.

[0100] Identification of genomic DNA and heterologous species DNA can be accomplished by standard DNA/DNA hybridization techniques, under appropriately stringent conditions, using all or part of a polynucleotide sequence as a probe to screen an appropriate library. Alternatively, PCR techniques using oligonucleotide primers that are designed based on known genomic DNA, cDNA and protein sequences can be used to amplify and identify genomic and cDNA sequences.

[0101] In another aspect, the present invention provides isolated polypeptides encoded by the above polynucleotides. As used herein, the term “polypeptide” encompasses amino acid chains of any length, including full-length proteins, wherein the amino acid residues are linked by covalent peptide bonds. The term “polypeptide encoded by a polynucleotide” as used herein, includes polypeptides encoded by a polynucleotide that comprises a partial isolated polynucleotide sequence provided herein. In specific embodiments, the inventive polypeptides comprise an amino acid sequence selected from the group consisting of SEQ ID NO: 45-88, as well as variants of such sequences.

[0102] As noted above, polypeptides of the present invention may be produced recombinantly by inserting a polynucleotide sequence encoding the polypeptide into an expression vector and expressing the polypeptide in an appropriate host. Any of a variety of expression vectors known to those of ordinary skill in the art may be employed. Expression may be achieved in any appropriate host cell that has been transformed or transfected with an expression vector containing a polynucleotide molecule that encodes a recombinant polypeptide. Suitable host cells include prokaryotes, yeast, and higher eukaryotic cells. Preferably, the host cells employed are plant, E. coli, insect, yeast, or a mammalian cell line such as COS or CHO. The polynucleotide sequences expressed in this manner may encode naturally occurring polypeptides, portions of naturally occurring polypeptides, or other variants thereof. The expressed polypeptides may be used in various assays known in the art to determine their biological activity. Such polypeptides may also be used to raise antibodies, to isolate corresponding interacting proteins or other compounds, and to quantitatively determine levels of interacting proteins or other compounds.

[0103] In a related aspect, polypeptides are provided that comprise at least a functional portion of a polypeptide having an amino acid sequence selected from the group consisting of sequences provided in SEQ ID NO: 45-88 and variants thereof. As used herein, the “functional portion” of a polypeptide is that portion which contains an active site essential for affecting the function of the polypeptide, for example, a portion of the molecule that is capable of binding one or more reactants. The active site may be made up of separate portions present on one or more polypeptide chains and will generally exhibit high binding affinity. Functional portions of a polypeptide may be identified by first preparing fragments of the polypeptide by either chemical or enzymatic digestion of the polypeptide, or by mutation analysis of the polynucleotide that encodes the polypeptide and subsequent expression of the resulting mutant polypeptides. The polypeptide fragments or mutant polypeptides are then tested to determine which portions retain biological activity, using methods well known to those of skill in the art, including the representative assays described below.

[0104] Portions and other variants of the inventive polypeptides may be generated by synthetic or recombinant means. Synthetic polypeptides having fewer than about 100 amino acids, and generally fewer than about 50 amino acids, may be generated using techniques well known to those of ordinary skill in the art. For example, such polypeptides may be synthesized using any of the commercially available solid-phase techniques, such as the Merrifield solid-phase synthesis method, where amino acids are sequentially added to a growing amino acid chain. See Merrifield, J. Am. Chem. Soc. 85: 2149-2146, 1963. Equipment for automated synthesis of polypeptides is commercially available from suppliers such as Perkin Elmer/Applied Biosystems, Inc. (Foster City, Calif.), and may be operated according to the manufacturer's instructions. Variants of a native polypeptide may be prepared using standard mutagenesis techniques, such as oligonucleotide-directed site-specific mutagenesis (Kunkel, Proc. Natl. Acad. Sci. USA 82: 488-492, 1985). Sections of DNA sequences may also be removed using standard techniques to permit preparation of truncated polypeptides.

[0105] As used herein, the term “variant” comprehends nucleotide or amino acid sequences different from the specifically identified sequences, wherein one or more nucleotides or amino acid residues is deleted, substituted, or added. Variants may be naturally occurring allelic variants, or non-naturally occurring variants. Variant sequences (polynucleotide or polypeptide) preferably exhibit at least 75%, more preferably at least 80%, more preferably at least 90%, more preferably yet at least 95%, and most preferably at least 98% identity to a sequence of the present invention. The percentage identity is determined by aligning the two sequences to be compared as described below, determining the number of identical residues in the aligned portion, dividing that number by the total number of residues in the inventive (queried) sequence, and multiplying the result by 100.

[0106] Polynucleotides and polypeptides having a specified percentage identity to a polynucleotide or polypeptide identified in one of SEQ ID NO: 1-88 thus share a high degree of similarity in their primary structure. In addition to a specified percentage identity to a polynucleotide of the present invention, variant polynucleotides and polypeptides preferably have additional structural and/or functional features in common with a polynucleotide of the present invention. Polynucleotides having a specified degree of identity to, or capable of hybridizing to, a polynucleotide of the present invention preferably additionally have at least one of the following features: (1) they contain an open reading frame, or partial open reading frame, encoding a polypeptide, or a functional portion of a polypeptide, having substantially the same functional properties as the polypeptide, or functional portion thereof, encoded by a polynucleotide in a recited SEQ ID NO:; or (2) they contain identifiable domains in common.

[0107] Polynucleotide or polypeptide sequences may be aligned, and percentages of identical nucleotides or amino acids in a specified region may be determined against another polynucleotide or polypeptide, using computer algorithms that are publicly available. For example, the BLASTN and FASTA algorithms, set to the default parameters described in the documentation and distributed with the algorithm, may be used for aligning and identifying the similarity of polynucleotide sequences. The alignment and similarity of polypeptide sequences may be examined using the BLASTP algorithm. BLASTX and FASTX algorithms compare nucleotide query sequences translated in all reading frames against polypeptide sequences. The FASTA and FASTX algorithms are described in Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85:2444-2448, 1988; and in Pearson, Methods in Enzymol. 183:63-98, 1990. The FASTA software package is available from the University of Virginia by contacting the Assistant Provost for Research, University of Virginia, PO Box 9025, Charlottesville, Va. 22906-9025. The BLASTN software is available from the National Center for Biotechnology Information (NCBI), National Library of Medicine, Building 38A, Room 8N805, Bethesda, Md. 20894. The BLASTN algorithm Version 2.0.11 [Jan. 20, 2000] set to the default parameters described in the documentation and distributed with the algorithm, is preferred for use in the determination of polynucleotide variants according to the present invention. The use of the BLAST family of algorithms, including BLASTN, BLASTP and BLASTX, is described in the publication of Altschul et al., “Gapped BLAST and PSI-BLAST: a new generation of protein database search programs,” Nucleic Acids Res. 25:3389-3402, 1997.

[0108] The following running parameters are preferred for determination of alignments and similarities using BLASTN that contribute to the percentage identity and E values for polynucleotides: Unix running command with the following default parameters: blastall -p blastn -d embldb -e 10-G 0 -E 0 -r 1 -v 30 -b 30 -i queryseq -o results; and parameters are: -p Program Name [String]; -d Database [String]; -e Expectation value (E) [Real]; -G Cost to open a gap (zero invokes default behavior) [Integer]; -FF low complexity filter; -E Cost to extend a gap (zero invokes default behavior) [Integer]; -r Reward for a nucleotide match (BLASTN only) [Integer]; -v Number of one-line descriptions (V) [Integer]; -b Number of alignments to show (B) [Integer]; -i Query File [File In]; -o BLAST report Output File [File Out] Optional.

[0109] The following running parameters are preferred for determination of alignments and similarities using BLASTP that contribute to the percentage identity and E values of polypeptide sequences: blastall -p blastp d swissprotdb -e 10 -G 0 -E 0 -v 30 -b 30 -i queryseq o results; the parameters are: -p Program Name [String]; -d Database [String]; -e Expectation value (E) [Real]; -G Cost to open a gap (zero invokes default behavior) [Integer]; -FF low complexity filter; -E Cost to extend a gap (zero invokes default behavior) [Integer]; -v Number of one-line descriptions (v) [Integer]; -b Number of alignments to show (b) [Integer]; -I Query File [File In]; -o BLAST report Output File [File Out] Optional.

[0110] The “hits” to one or more database sequences by a queried sequence produced by BLASTN, BLASTP, FASTA or a similar algorithm, align and identify similar portions of sequences. The hits are arranged in order of the degree of similarity and the length of sequence overlap. Hits to a database sequence generally represent an overlap over only a fraction of the sequence length of the queried sequence.

[0111] As noted above, the percentage identity of a polynucleotide or polypeptide sequence is determined by aligning polynucleotide and polypeptide sequences using appropriate algorithms, such as BLASTN or BLASTP, respectively, set to default parameters; identifying the number of identical nucleic or amino acids over the aligned portions; dividing the number of identical nucleic or amino acids by the total number of nucleic or amino acids of the polynucleotide or polypeptide of the present invention; and then multiplying by 100 to determine the percentage identity. By way of example, a queried polynucleotide having 220 nucleic acids has a hit to a polynucleotide sequence in the EMBL database having 520 nucleic acids over a stretch of 23 nucleotides in the alignment produced by the BLASTN algorithm using the default parameters. The 23-nucleotide hit includes 21 identical nucleotides, one gap and one different nucleotide. The percentage identity of the queried polynucleotide to the hit in the EMBL database is thus 21/220 times 100, or 9.5%. The percentage identity of polypeptide sequences may be determined in a similar fashion.

[0112] The BLASTN and BLASTX algorithms also produce “Expect” values for polynucleotide and polypeptide alignments. The Expect value (E) indicates the number of hits one can “expect” to see over a certain number of contiguous sequences by chance when searching a database of a certain size. The Expect value is used as a significance threshold for determining whether the hit to a database indicates true similarity. For example, an E value of 0.1 assigned to a polynucleotide hit is interpreted as meaning that in a database of the size of the EMBL database, one might expect to see 0.1 matches over the aligned portion of the sequence with a similar score simply by chance. By this criterion, the aligned and matched portions of the sequences then have a probability of 90% of being related. For sequences having an E value of 0.01 or less over aligned and matched portions, the probability of finding a match by chance in the EMBL database is 1% or less using the BLASTN algorithm. E values for polypeptide sequences may be determined in a similar fashion using various polypeptide databases, such as the SwissProt database.

[0113] According to one embodiment, “variant” polynucleotides and polypeptides, with reference to each of the polynucleotides and polypeptides of the present invention, preferably comprise sequences having the same number or fewer nucleotides or amino acids than each of the polynucleotides or polypeptides of the present invention and producing an E value of 0.01 or less when compared to the polynucleotide or polypeptide of the present invention. That is, a variant polynucleotide or polypeptide is any sequence that has at least a 99% probability of being related to the polynucleotide or polypeptide of the present invention, measured as having an E value of 0.01 or less using the BLASTN or BLASTX algorithms set at the default parameters. According to a preferred embodiment, a variant polynucleotide is a sequence having the same number or fewer nucleic acids than a polynucleotide of the present invention that has at least a 99% probability of being related to the polynucleotide of the present invention, measured as having an E value of 0.01 or less using the BLASTN algorithm set at the default parameters. Similarly, according to a preferred embodiment, a variant polypeptide is a sequence having the same number or fewer amino acids than a polypeptide of the present invention that has at least a 99% probability of being related as the polypeptide of the present invention, measured as having an E value of 0.01 or less using the BLASTP algorithm set at the default parameters.

[0114] In an alternative embodiment, variant polynucleotides are sequences that hybridize to a polynucleotide of the present invention under stringent conditions. Stringent hybridization conditions for determining complementarity include salt conditions of less than about 1 M, more usually less than about 500 mM, and preferably less than about 200 mM. Hybridization temperatures can be as low as 5° C., but are generally greater than about 22° C., more preferably greater than about 30° C., and most preferably greater than about 37° C. Longer DNA fragments may require higher hybridization temperatures for specific hybridization. Since the stringency of hybridization may be affected by other factors such as probe composition, presence of organic solvents, and extent of base mismatching, the combination of parameters is more important than the absolute measure of any one alone. An example of “stringent conditions” is prewashing in a solution of 6×SSC, 0.2% SDS; hybridizing at 65° C., 6×SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in 1×SSC, 0.1% SDS at 65° C. and two washes of 30 minutes each in 0.2×SSC, 0.1% SDS at 65° C.

[0115] The present invention also encompasses polynucleotides that differ from the disclosed sequences but that, as a consequence of the discrepancy of the genetic code, encode a polypeptide having similar enzymatic activity to a polypeptide encoded by a polynucleotide of the present invention. Thus, polynucleotides comprising sequences that differ from the polynucleotide sequences recited in SEQ ID NO: 1-44, or complements, reverse sequences, or reverse complements of those sequences, as a result of conservative substitutions are contemplated by and encompassed within the present invention. Additionally, polynucleotides comprising sequences that differ from the polynucleotide sequences recited in SEQ ID NO: 1-44, or complements, reverse complements or reverse sequences thereof, as a result of deletions and/or insertions totaling less than 10% of the total sequence length are also contemplated by and encompassed within the present invention. Similarly, polypeptides comprising sequences that differ from the polypeptide sequences recited in SEQ ID NO: 45-88 as a result of amino acid substitutions, insertions, and/or deletions totaling less than 10% of the total sequence length are contemplated by and encompassed within the present invention, provided the variant polypeptide has activity in a fructan, cellulose, starch and/or tannin biosynthetic pathway.

[0116] In another aspect, the present invention provides genetic constructs comprising, in the 5′-3′ direction, a gene promoter sequence; an open reading frame coding for at least a functional portion of a polypeptide of the present invention; and a gene termination sequence. The open reading frame may be orientated in either a sense or anti-sense direction. For applications where amplification of fructan, cellulose, starch or tannin synthesis is desired, the open reading frame may be inserted in the construct in a sense orientation, such that transformation of a target organism with the construct will lead to an increase in the number of copies of the gene and therefore an increase in the amount of enzyme. When down-regulation of fructan, cellulose, starch or tannin synthesis is desired, the open reading frame may be inserted in the construct in an anti-sense orientation, such that the RNA produced by transcription of the polynucleotide is complementary to the endogenous mRNA sequence. This, in turn, will result in a decrease in the number of copies of the gene and therefore a decrease in the amount of enzyme. Alternatively, regulation may be achieved by inserting appropriate sequences or subsequences (e.g., DNA or RNA) in ribozyme constructs.

[0117] Genetic constructs comprising a non-coding region of a gene coding for a polypeptide of the present invention, or a nucleotide sequence complementary to a non-coding region, together with a gene promoter sequence and a gene termination sequence, are also provided. As used herein the term “non-coding region” includes both transcribed sequences which are not translated, and non-transcribed sequences within about 2000 base pairs 5′ or 3′ of the translated sequences or open reading frames. Examples of non-coding regions which may be usefully employed in the inventive constructs include introns and 5′-non-coding leader sequences. Transformation of a target plant with such a genetic construct may lead to a reduction in the amount of fructan, cellulose, starch or tannin synthesized by the plant by the process of cosuppression, in a manner similar to that discussed, for example, by Napoli et al., Plant Cell 2:279-290, 1990; and de Carvalho Niebel et al., Plant Cell 7:347-358, 1995.

[0118] The genetic constructs of the present invention further comprise a gene promoter sequence and a gene termination sequence, operably linked to the polynucleotide to be transcribed, which control expression of the gene. The gene promoter sequence is generally positioned at the 5′ end of the polynucleotide to be transcribed, and is employed to initiate transcription of the polynucleotide. Gene promoter sequences are generally found in the 5′ non-coding region of a gene but they may exist in introns (Luehrsen, Mol Gen. Genet. 225:81-93, 1991) or in the coding region, as for example in PAL of tomato (Bloksberg, Studies on the Biology of Phenylalanine Ammonia Lyase and Plant Pathogen Interaction, Ph. D. Thesis, University of California, Davis, 1991, University Microfilms International Order No. 9217564). When the construct includes an open reading frame in a sense orientation, the gene promoter sequence also initiates translation of the open reading frame. For genetic constructs comprising either an open reading frame in an anti-sense orientation or a non-coding region, the gene promoter sequence consists only of a transcription initiation site having a RNA polymerase binding site.

[0119] A variety of gene promoter sequences which may be usefully employed in the genetic constructs of the present invention are well known in the art. The promoter gene sequence, and also the gene termination sequence, may be endogenous to the target plant host or may be exogenous, provided the promoter is functional in the target host. For example, the promoter and termination sequences may be from other plant species, plant viruses, bacterial plasmids and the like. Preferably, gene promoter and termination sequences are from the inventive sequences themselves.

[0120] Factors influencing the choice of promoter include the desired tissue specificity of the construct, and the timing of transcription and translation. For example, constitutive promoters, such as the 35S Cauliflower Mosaic Virus (CaMV 35S) promoter or the superubiquitin promoter (PCT International Patent Publication WO 00/58474), will affect the activity of the enzyme in all parts of the plant. Use of a tissue specific promoter will result in production of the desired sense or anti-sense RNA only in the tissue of interest. With DNA constructs employing inducible gene promoter sequences, the rate of RNA polymerase binding and initiation can be modulated by external stimuli, such as light, heat, anaerobic stress, alteration in nutrient conditions and the like. Temporally regulated promoters can be employed to effect modulation of the rate of RNA polymerase binding and initiation at a specific time during development of a transformed cell. Preferably, the original promoters from the enzyme gene in question, or promoters from a specific tissue-targeted gene in the organism to be transformed, such as Lolium or Festuca, are used. Grass promoters different from the original gene may also be usefully employed in the inventive genetic constructs in order to prevent feedback inhibition. For example, the fructosyltransferase gene will be regulated by sucrose sensing systems; therefore removing the gene from under control of its normal promoter allows the gene to be active all the time. Other examples of gene promoters which may be usefully employed in the present invention include, mannopine synthase (mas), octopine synthase (ocs) and those reviewed by Chua et al., Science 244:174-181, 1989.

[0121] The gene termination sequence, which is located 3′ to the polynucleotide to be transcribed, may come from the same gene as the gene promoter sequence or may be from a different gene. Many gene termination sequences known in the art may be usefully employed in the present invention, such as the 3′ end of the Agrobacterium tumefaciens nopaline synthase gene. However, preferred gene terminator sequences are those from the original enzyme gene or from the target species to be transformed.

[0122] The genetic constructs of the present invention may also contain a selection marker. that is effective in plant cells, to allow for the detection of transformed cells containing the inventive construct. Such markers, which are well known in the art, typically confer resistance to one or more toxins. One example of such a marker is the NPTII gene whose expression results in resistance to kanamycin or hygromycin, antibiotics which are usually toxic to plant cells at a moderate concentration (Rogers et al., in Weissbach A and H, eds., Methods for Plant Molecular Biology, Academic Press Inc.: San Diego, Calif., 1988). Alternatively, the presence of the desired construct in transformed cells can be determined by means of other techniques well known in the art, such as Southern and Western blots.

[0123] Techniques for operatively linking the components of the inventive genetic constructs are well known in the art and include the use of synthetic linkers containing one or more restriction endonuclease sites as described, for example, by Sambrook et al., (Molecular cloning: a laboratory manual, CSHL Press: Cold Spring Harbor, N.Y., 1989). The genetic construct of the present invention may be linked to a vector having at least one replication system, for example, E. coli, whereby after each manipulation, the resulting construct can be cloned and sequenced, and the correctness of the manipulation determined.

[0124] The genetic constructs of the present invention may be used to transform a variety of plants, both monocotyledonous (e.g., grasses, maize/corn, grains, oats, rice, sorghum, millet, rye, sugar cane, wheat and barley), dicotyledonous (e.g., Arabidopsis, tobacco, legumes, alfalfa, oaks, eucalyptus, maple), and gymnosperms. In a preferred embodiment, the inventive genetic constructs are employed to transform grasses. Preferably the target plant is selected from the group consisting of Lolium and Festuca species, most preferably from the group consisting of Lolium perenne and Festuca arundinacea. Plants that may be usefully transformed with the inventive genetic constructs include other species of ryegrass and fescue, including, but not limited to Lolium multiflorum (Italian ryegrass), Lolium hybridum (hybrid ryegrass), Lolium rigidum (Wimerra grass), Lolium temulentum (darnel), Festuca rubra (red fescue) and Festuca pratensis (meadow fescue). As discussed above, transformation of a plant with a genetic construct of the present invention will produce a modified fructan, cellulose, starch or tannin content in the plant.

[0125] The production of RNA in target cells may be controlled by choice of the promoter sequence, or by selecting the number of functional copies or the site of integration of the polynucleotides incorporated into the genome of the target organism. A target plant may be transformed with more than one construct of the present invention, thereby modulating the fructan, cellulose, starch and/or tannin biosynthetic pathways by affecting the activity of more than one enzyme, affecting enzyme activity in more than one tissue, or affecting enzyme activity at more than one expression time. Similarly, a construct may be assembled containing more than one open reading frame coding for an enzyme encoded by a polynucleotide of the present invention or more than one non-coding region of a gene coding for such an enzyme. The polynucleotides of the present invention may also be employed in combination with other known sequences encoding enzymes involved in the lignin, fructan and/or tannin biosynthetic pathways. In this manner, more than one biosynthetic pathway may be modulated, or a fructan, cellulose, starch or tannin biosynthetic pathway may be added to a plant to produce a plant having an altered phenotype.

[0126] Techniques for stably incorporating genetic constructs into the genome of target plants are well known in the art and include Agrobacterium tumefaciens mediated introduction, electroporation, protoplast fusion, injection into reproductive organs, injection into immature embryos, high velocity projectile introduction and the like. The choice of technique will depend upon the target plant to be transformed. For example, dicotyledonous plants, and certain monocots and gymnosperms may be transformed by Agrobacterium Ti plasmid technology, as described, for example by Bevan, Nucleic Acid Res. 12:8711-8721, 1984. Targets for the introduction of the genetic constructs of the present invention include tissues, such as leaf tissue, disseminated cells, protoplasts, seeds, embryos, meristematic regions; cotyledons, hypocotyls, and the like. Transformation techniques which may be usefully employed in the inventive methods include those taught by Ellis et al.,Plant Cell Reports, 8:16-20, 1989; Wilson et al., Plant Cell Reports 7:704-707, 1989; and Tautorus et al., Theor. Appl. Genet. 78:531-536, 1989.

[0127] Once the cells are transformed, cells having the inventive genetic construct incorporated in their genome may be selected by means of a marker, such as the kanamycin resistance marker discussed above. Transgenic cells may then be cultured in an appropriate medium to regenerate whole plants, using techniques well known in the art. In the case of protoplasts, the cell wall is allowed to reform under appropriate osmotic conditions. In the case of seeds or embryos, an appropriate germination or callus initiation medium is employed. For explants, an appropriate regeneration medium is used. Regeneration of plants is well established for many species. The resulting transformed plants may be reproduced sexually or asexually, using methods well known in the art, to give successive generations of transgenic plants.

[0128] Polynucleotides of the present invention may also be used to specifically suppress gene expression by methods that operate post-transcriptionally to block the synthesis of products of targeted genes, such as RNA interference (RNAi), and quelling. Briefly, traditional methods of gene suppression, employing anti-sense RNA or DNA, operate by binding to the reverse sequence of a gene of interest such that binding interferes with subsequent cellular processes and therefore blocks synthesis of the corresponding protein. RNAi also operates on a post-translational level and is sequence specific, but suppresses gene expression far more efficiently. Exemplary methods for controlling or modifying gene expression using RNAi are provided in U.S. Pat. No. 6,506,559 and PCT International Publications WO 99/49029 and WO 99/53050. In these methods, post-transcriptional gene silencing is brought about by a sequence-specific RNA degradation process which results in the rapid degradation of transcripts of sequence-related genes. Studies have shown that double-stranded RNA may act as a mediator of sequence-specific gene silencing (see, for example, Montgomery and Fire, Trends in Genetics, 14:255-258, 1998). Gene constructs that produce transcripts with self-complementary regions are particularly efficient at gene silencing. A unique feature of this post-transcriptional gene silencing pathway is that silencing is not limited to the cells where it is initiated. The gene-silencing effects may be disseminated to other parts of an organism and even transmitted through the germ line to several generations.

[0129] The polynucleotides of the present invention may thus be employed to generate gene silencing constructs and/or gene-specific self-complementary RNA sequences that can be delivered by conventional art-known methods to plant tissues, such as forage grass tissues. Within genetic constructs, sense and antisense sequences can be placed in regions flanking an intron sequence in proper splicing orientation with donor and acceptor splicing sites, such that intron sequences are removed during processing of the transcript, and sense and antisense sequences, as well as splice junction sequences, bind together to form double-stranded RNA. Alternatively, spacer sequences of various lengths may be employed to separate self-complementary regions of sequence in the construct. During processing of the gene construct transcript, intron sequences are spliced-out, allowing sense and anti-sense sequences, as well as splice junction sequences, to bind forming double-stranded RNA. Select ribonucleases then bind to and cleave the double-stranded RNA, thereby initiating the cascade of events leading to degradation of specific MRNA gene sequences, and silencing specific genes. Alternatively, rather than using a gene construct to express the self-complementary RNA sequences, the gene-specific double-stranded RNA segments are delivered to one or more targeted areas to be internalized into the cell cytoplasm to exert a gene silencing effect. The double-stranded RNA must have sufficient homology to the targeted gene to mediate RNAi and is preferably at least 25 nucleotides in length. Preferably, the double-stranded RNA corresponds specifically to a polynucleotide of the present invention. Gene silencing RNA sequences comprising the polynucleotides of the present invention are useful for creating genetically modified plants with desired phenotypes as well as for characterizing genes (for example, in high-throughput screening of sequences), and studying their functions in intact organisms.

EXAMPLE 1 Isolation of cDNA Sequences from L. Perenne and F. Arundinacea cDNA Libraries

[0130]L. perenne and F. arundinacea cDNA expression libraries were constructed and screened as follows. Tissue was collected from L. perenne and F. arundinacea during winter and spring, and snap-frozen in liquid nitrogen. The tissues collected included those obtained from leaves, pseudostem, roots, inflorescence (day 0), stem bases from day 7 emerged inflorescence, basal leaf day 3 and day 6, floral stem and vegetative stem. Total RNA was isolated from each tissue type using TRIzol Reagent (BRL Life Technologies, Gaithersburg, Md.). mRNA from each tissue type was obtained using a Poly(A) Quik mRNA isolation kit (Stratagene, La Jolla, Calif.), according to the manufacturer's specifications. cDNA expression libraries were constructed from the purified mRNA by reverse transcriptase synthesis followed by insertion of the resulting cDNA in Lambda ZAP using a ZAP Express cDNA Synthesis Kit (Stratagene), according to the manufacturer's protocol. The resulting cDNA clones were packaged using a Gigapack II Packaging Extract (Stratagene) employing 1 μl of sample DNA from the 5 μl ligation mix. Mass excision of the libraries was performed using XL1-Blue MRF′ cells and XLOLR cells (Stratagene) with ExAssist helper phage (Stratagene). The excised phagemids were diluted with NZY broth (Gibco BRL, Gaithersburg, Md.) and plated out onto LB-kanamycin agar plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactosidase (X-gal) and isopropylthio-beta-galactoside (IPTG).

[0131] Of the colonies plated and picked for DNA preparations, the large majority contained an insert suitable for sequencing. Positive colonies were cultured in NZY broth with kanamycin and DNA was purified following standard protocols. Agarose gel at 1% was used to screen sequencing templates for chromosomal contamination. Dye terminator sequences were prepared using a Biomek 2000 robot (Beckman Coulter Inc., Fullerton, Calif.) for liquid handling and DNA amplification using a 9700 PCR machine (Perkin Elmer/Applied Biosystems, Foster City, Calif.) according to the manufacturer's protocol.

[0132] The DNA sequences for positive clones were obtained using a Perkin Elmer/Applied Biosystems Division Prism 377 sequencer. cDNA clones were sequenced from the 5′ end. The polynucleotide sequences identified as SEQ ID NO: 1, 3-5, 8-15, 17, 18, 20, 25, 27, 28, 30, 36-39 and 44 were identified from Lolium perenne cDNA expression libraries, with the polynucleotides of SEQ ID NO: 2, 6, 7, 16, 19, 21-24, 26, 29, 31-35 and 40-43 being identified from Festuca arundinacea cDNA expression libraries.

[0133] BLASTN Polynucleotide Analysis

[0134] The isolated cDNA sequences were compared to sequences in the EMBL DNA database using the computer algorithm BLASTN. Comparisons of DNA sequences provided in SEQ ID NO: 1-44 to sequences in the EMBL DNA database were made as of Apr. 28, 2003, using BLASTN algorithm Version 2.0.11 [Jan. 20, 2000], and the following Unix running command: blastall -p blastn -d embldb -e 10 -FF -G0 -E0 -r 1 -v 30 -b 30 -i queryseq -o.

[0135] The sequences of SEQ ID NO: 6-9, 11-19, 21, 25-27 and 34-44 were determined to have less than 50% identity, determined as described above using the computer algorithm BLASTN, to sequences in the EMBL database. The sequence of SEQ ID NO: 3, 4, 10, 20, 22-24, 28, 29 and 31-33 was determined to have less than 75% identity, determined as described above, to sequences in the EMBL database. The sequences of SEQ ID NO: 1, 2 and 30 were determined to have less than 90% identity to sequences in the EMBL database using the computer algorithm BLASTN, as described above. Finally, the sequence of SEQ ID NO: 5 were determined to have less than 98% identity to sequences in the SwissProt database using the computer algorithm BLASTP, as described above.

[0136] BLASTP Polypeptide Analysis

[0137] The isolated sequences were compared to sequences in the SwissProt protein database using the computer algorithm BLASTP. Specifically, comparisons of polypeptide sequences provided in SEQ ID NO: 45-88 to sequences in the SwissProt protein database were made as of Apr. 28, 2003, using BLASTP algorithm Version 2.0.11 [Jan. 20, 2000], and the following Unix running command: blastall -p blastn -d embldb -e 10 -FF -G0 -E0 -v 30 -b 30 -i queryseq -o.

[0138] The sequences of SEQ ID NO: 78-81 were determined to have less than 50% identity to sequences in the SwissProt database using the computer algorithm BLASTP as described above. The sequences of SEQ ID NO: 51, 53, 55, 56, 71, 83 and 88 were determined to have less than 75% identity to sequences in the SwissProt database using the computer algorithm BLASTP, as described above. The sequences of SEQ ID NO: 50, 52, 54, 57-68, 82 and 84-87 were determined to have less than 90% identity to sequences in the SwissProt database using the computer algorithm BLASTP, as described above. Finally, the sequences of SEQ ID NO: 45-49, 69, 70 and 72-77 were determined to have less than 98% identity to sequences in the SwissProt database using the computer algorithm BLASTP, as described above.

[0139] BLASTX Polynucleotide Analysis

[0140] The isolated cDNA sequences were compared to sequences in the SwissProt protein DNA database using the computer algorithm BLASTX. Comparisons of DNA sequences provided in SEQ ID NOS: 1-44, to sequences in the SwissProt DNA database (using BLASTX) were made as of Apr. 28, 2003, using BLAST algorithm Version 2.0.11 [Jan. 20, 2000], and the following Unix running command: blastall p blastn -d embldb -e 10 -FF -G0 -E0 -r 1 -v 30 -b 30 -i queryseq o.

[0141] The sequences of SEQ ID NO: 27 and 34-37 were determined to have less than 50% identity, determined as described above, to sequences in the SwissProt database using the computer algorithm BLASTX, as described above. The sequences of SEQ ID NO: 3, 4, 6-19, 21-26, 28, 29, 33 and 38-44 were determined to have less than 75% identity, determined as described above, to sequences in the SwissProt database using the computer algorithm BLASTX, as described above. Finally, the sequences of SEQ ID NO: 1, 2, 5, 20 and 30-32 were determined to have less than 90% identity, determined as described above, to sequences in the SwissProt database using the computer algorithm BLASTX, as described above.

[0142] FIGS. 3-41 show the positions of domains within the amino acid sequences of SEQ ID NO: 45-48, 53-70 and 72-88, respectively. These domains were determined with InterProScan software Release v3.1, Nov. 6, 2001. The InterPro database integrates PROSITE, PRINTS, Pfam, ProDom, SMART and TIGRFAMs databases, and the addition of others is scheduled. InterPro data is distributed in XML format and it is freely available under the InterPro Consortium copyright. The European Bioinformatics Institute (EBI) is a non-profit academic organization that forms part of the European Molecular Biology Laboratory (EMBL): Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SD UK.

EXAMPLE 2 Use of Chalcone Synthase Genes to Modify Tannin Biosynthesis

[0143] Certain Arabidopsis mutants of the transparent testa (tt) phenotype do not make the anthocyanin pigment cyanidin and therefore have no seed coat color. The genes responsible for many of these mutants have now been identified as shown in Table 3. TABLE 3 Enzyme Abbreviation Locus Chromosome Dihydroflavanol-4-reductase DFR tt3 5 Chalcone synthase CHS tt4 5 Chalcone isomerase CHI tt5 3 Flavanone 3-β-hydroxylase F3βH tt6 3

[0144] Over-expression of the maize gene for CHS has been shown to complement the Arabidopsis tt4 mutant, thereby restoring cyanidin synthesis and seed coat color (Dong et al., Plant Physiol. 127:46-57, 2001). Complementation of these Arabidopsis mutants may therefore be employed to demonstrate the function of the inventive polynucleotides encoding enzymes involved in the tannin biosynthetic pathway.

[0145] Two chalcone synthase genes were identified from F. arundinacea (SEQ ID NO: 32 and 33). Sense constructs containing a polynucleotide including the coding region of one chalcone synthase gene, FaCHS2, (SEQ ID NO: 33) under the control of the CaMV 35S promoter were inserted into a binary vector and used to transform Agrobacterium tumefaciens LBA4404 using published methods (see, An G, Ebert PR, Mitra A, Ha SB, “Binary Vectors,” in Gelvin SB, Schilperoort RA, eds., Plant Molecular Biology Manual, Kluwer Academic Publishers: Dordrecht, 1988). The presence and integrity of the binary vector in A. tumefaciens was verified by polymerase chain reaction (PCR) using the forward primer provided in SEQ ID NO: 89 and reverse primer provided in SEQ ID: 90.

[0146] The A. tumefaciens containing the sense gene construct were used to transform Arabidopsis tt4 mutants by floral dipping (Clough and Bent, Plant J. 16:735-743, 1998) and several independent transformed plant lines were established for the sense. Transformed plants containing the appropriate tannin gene construct were verified using PCR.

[0147] The presence of cyanidin in the transformed plants is demonstrated by a phenotypic change in plant seedling color and by analyzing cyanidin extracts made from transgenic plants grown under stressed conditions (Dong et al., Plant Physiol. 127:46-57, 2001). Briefly, cyanidins are extracted from plant tissue with an acid/alcohol solution (HCl/methanol) and water. Chlorophyll is removed by freezing the extracts followed by centrifugation at 4° C. at 20,000×g for 20 min. Any remaining chlorophyll is removed through a chloroform extraction. The absorbance at 530 nm is measured for each of the cyanidin extracts. Non-transgenic wild type and control Arabidopsis plants are used as controls.

EXAMPLE 3 Use of Sucrose Transporters to Complement a Yeast Strain Unable to Grow on Sucrose

[0148] Two Lolium perenne genes, LpSUT2 (SEQ ID: 25) and LpSUT-like (SEQ ID: 27), and two Festuca arundinacea genes, FaSUT1 (SEQ ID NO: 22) and FaSUT2 (SEQ ID NO: 26) share amino acid sequence identity with sucrose transporter (SUT1 and SUT2) genes from other plant species (Barker et al., Plant Cell 12:1153-1164, 2000; Weise et al., Plant Cell 12:1345-55, 2000; Lemoine R., Biochim Biophys Acta 1465:246-62, 2000). The first plant gene encoding a sucrose carrier protein, from spinach, was isolated by functional expression in a yeast strain, SUSY7 (Riesmeier et al., EMBO J. 11:4705-4713).

[0149] The gene of SEQ ID NO: 27 was digested and cloned into the yeast expression vector pYEP 112 A1 NE for functional complementation using this yeast system. Plasmid DNA was verified by sequencing and transformed into S. cerevisiae strain SUSY7, which had been engineered to express cytosolic sucrose synthase enabling it to metabolize sucrose entering the cell. Constitutive expression of the grass sucrose transporters within this yeast strain facilitated transport of sucrose in to the cell and its growth on sucrose minimal media. Growth rates of recombinant and wild type yeast strains in both sucrose and glucose minimal media were measured.

[0150] Results showed that the yeast strain containing the gene of SEQ ID NO: 27 was able to grow on sucrose minimal medium because the constitutive expression of the SUT-like gene within this yeast strain facilitated transport of sucrose into the cell.

EXAMPLE 4 Use of Alkaline/neutral Invertases to Cleave Sucrose

[0151] A number of Lolium perenne and Festuca arundinacea genes (SEQ ID NO: 5, 7 and 9-14) were identified that share amino acid sequence identity with alkaline/neutral invertase genes from other plant species (Sturm et al., Physiol. Planta 107:159-165, 1999; Gallagher and Pollock, J. Exp. Bot. 49:789-795, 1998).

[0152]L. perenne gene AN_INV8 (SEQ ID NO: 12) was amplified by PCR from the start methionine using forward (SEQ ID NO: 91) and reverse (SEQ ID NO: 92) primers, then cloned into the pET41a expression plasmid. The resulting plasmid was transformed into E. coli BL21 cells using standard protocols, and protein expression induced using IPTG. The soluble recombinant protein was assayed for its ability to cleave sucrose. Cells were lysed in citrate buffer and the soluble protein incubated with 50 mM sucrose in citrate buffer pH7. Reactions were terminated by boiling. Cleavage of the sucrose by neutral invertase activity was determined by the formation of glucose in this reaction. Levels of glucose were determined with a Glucose HK assay kit GAHK-20 (Sigma, St Louis Mo.) utilizing hexokinase coupled to glucose-6-phosphate dehydrogenase, and reduction of NAD measured by absorbance at 340 nm.

[0153]FIG. 1 shows the invertase activity of recombinant AN_INV8 protein, measured as the amount (in μg) of glucose release from cleavage of sucrose per hour at pH7, and that of an empty vector (pET41a) control sample. The results showed that the purified protein released 35 μg of glucose per hour through the invertase cleavage of sucrose. No release was measured with the empty vector control.

EXAMPLE 5 Use of Pyrophosphate-dependent Phosphofructokinase to Phosphorylate Fructoes-6-Phosphate

[0154] Two Lolium perenne genes, LpPFPA (SEQ ID: 15) and LpPFPB (SEQ ID NO: 18), and two Festuca arundinacea genes, FaPFPA (SEQ ID NO: 16) and FaPFPB (SEQ ID NO: 19) share amino acid sequence identity with the A and B subunits of pyrophosphate-dependent phosphofructoskinase genes (PFP) from other plant species (Todd et al., Gene 152:181-6, 1995; Carlisle et al., J. Biol. Chem. 265:18366-71, 1990).

[0155] The entire coding regions were cloned into expression vector pBK-CMV, under the control of the CMV promoter for expression of recombinant protein in mammalian cells. The PFPA and PFPB genes from Lolium perenne or Festuca arundinacea were co-transfected in to mammalian HEK293T cells and protein extracted 48 hours later. Protein was also extracted from cells transfected with a negative control vector containing the β-galactosidase gene. Purified potato PFP (Sigma, St. Louis Mo.) was used as positive control. Activity of the PFP enzyme was measured spectrophotometrically by a decrease NADH and absorbance at 340 nm in a coupled reaction as described previously (Theodorou and Kruger, Planta 213:147-157, 2001). Briefly, the conversion of fructose-6-phosphate to fructose-1,6-diphosphate in the presence of activator, fructose-2,6-diphosphate was initiated by the addition of pyrophosphate and measured in a coupled reaction with aldolase, triose phosphate isomerase and glycerophosphate dehydrogenase.

[0156]FIG. 2 shows the PFP activity of the purified protein (conversion of fructose-6-phosphate to fructose-1-6-diphosphate) measured as conversion of PPi to inorganic phosphate. No conversion was obtained with the β-galactosidase negative control.

EXAMPLE 6 Use of Sucrose Phosphate Synthase Enzymes to Synthesize Sucrose

[0157] A Lolium perenne polynucleotide sequence (SEQ ID NO: 20) and a F. arundinacea polynucleotide sequence (SEQ ID NO: 21) have been identified that share identity with sucrose phosphate synthase (SPS) from other plant species. These genes are expressed in E. coli or Pichia using standard protocols, and the resulting purified protein assayed for its ability to synthesize sucrose from fructose-6-phosphate and uridine 5′-diphosphoglucose. Sucrose is detected by adding NAD and UDP-Glucose dehydrogenase, followed by the addition of anthrone reagent and then measuring the change in absorbance at 620 nm (Botha and Black, Aust. J. Plant Physiol. 27:81-85, 2000).

[0158] SEQ ID NOS: 1-88 are set out in the attached Sequence Listing. The codes for nucleotide sequences used in the attached Sequence Listing, including the symbol “n,” conform to WIPO Standard ST. 25 (1998), Appendix 2, Table 1.

[0159] All references cited herein, including patent references and non-patent publications, are hereby incorporated by reference in their entireties.

[0160] While in the foregoing specification this invention has been described in relation to certain preferred embodiments, and many details have been set forth for purposes of illustration, it will be apparent to those skilled in the art that the invention is susceptible to additional embodiments and that certain of the details described herein may be varied considerably without departing from the basic principles of the invention.

1 92 1 1737 DNA Lolium perenne 1 gctgaaatct cctcccgtcc tctcctcctc ctcctccgct tctccaaccc tcctccaccg 60 cccggatcgc gatggccgcc gccgccgtcg cccccgacgc gaagatcgag aagttccgcg 120 acgccgtcgc caagctcggc gagatcagcg agaacgagaa ggccggctgc atcagcctcg 180 tctcgcgcta cctcagcggc gaggcggagc agatcgagtg gagcaagatc cagaccccca 240 ccgacgaggt cgtcgtgccg tacgacaccc tcgcgcccgc gcccgaagat ctcgacgcca 300 tgaaggcgct gctcgacaag ctcgtcgtgc tcaagctcaa cggaggcctc ggcaccacca 360 tgggctgcac cggccccaag tctgttattg aagttcgcaa tgggtttaca tttcttgacc 420 ttattgtgat tcagattgag tccctgaaca agaagtacgg atgtgatgtc cctttacttc 480 tcatgaactc cttcaacacg catgatgata ctcaaaagat tgttgagaag tactccaact 540 ccaacatcaa catccacact ttcaaccaga gccaatatcc caggattgtt actgaagact 600 tcttgccact tccgagcaaa gggcagtcag gaaaggatgg ctggtatccc ccaggccatg 660 gcgatgtttt cccctctttg aacaacagtg gaaaacttga taccttattg tcgcagggca 720 aggagtatgt ctttgttgcc aactcagaca acttgggtgc tatagttgac atcaagatat 780 tgaaccacct gatcaacaac aagaatgaat actgcatgga ggttactccg aaaacattgg 840 ctgatgttaa aggtggcacc ctcatctcat atgaaggaag ggtccagctc ttggagattg 900 cccaagtccc tgatgagcat gtgaatgaat tcaagtcaat tgagaagttc aagatattca 960 ataccaacaa cctgtgggtg aacttgaagg cgatcaagag gcttgttgaa gctgatgcac 1020 ttaagatgga gatcattccc aaccctaagg aagttgatgg cgtgaaagtc cttcagctag 1080 aaactgcagc tggggcagcg attcggttct ttgataacgc aatcggcatc aacggtcccc 1140 gctcaaggtt tctgcccgtg aaggctacat cagatttgtt gcttgtgcag tctgacctct 1200 ataccttggt cgatggctat gtcatccgca acccagctag agtgaagcct tcaaaccctt 1260 ccattgagct tggtcctgag ttcaagaagg tcgccagttt cctggcccgg ttcaagtcaa 1320 tccccagcat cgttgagctc gacagcttga aggtctctgg tgatgtctcg tttggctctg 1380 gcatcgtact caagggcaac gtgaccatcg ctgccaagtc tggagtcaag ctggagatcc 1440 cagacggagc tgtgcttgag aacaaggaca tcaacggccc agaggatctt tgagcgacgc 1500 tcactcgcca ccgccagacg catcccggaa gccttccagt tctccttccc tgagttaaca 1560 acagtcttgt aattttcgtg tgcattctgc cgtggggtcg tcctgtggga gcccgtttat 1620 acagaataat tgtaatcccc tctgtccatc tgcacttctg ttcttcctgg gtggaaccag 1680 ggacgtaaag ttcttttggt gaaatgatat gccatagttt tattttcaaa aaaaaaa 1737 2 1697 DNA Festuca arundinacea 2 gctgaaatct cctcccgtcc tctcctcctc cgcttctcca agcctcctcc tccgcccgga 60 tcgcgatggc cgccgtcgcc gccgacgcga agatcgagaa gttccgcgac gccgtcgcca 120 agctcgacga gatcagcgag aacgagaagg ccgggtgcat cagcctcgtc tcgcgctacc 180 tcagcggtga ggcggagcag atcgagtgga gcaagatcca gacccccacc gacgaggtcg 240 tggtgcccta cgacaccctc gcgcccgcgc cccaagatct cgacgccatg aaggcgctgc 300 tcgacaagct cgtcgtgctc aagctcaacg gaggcctcgg caccaccatg ggctgcaccg 360 gtcccaagtc tgttattgaa gttcgcaatg ggtttacatt tcttgacctt attgtgattc 420 agatcgagtc cctgaacaag aagtacggat gtgatgtccc tttacttctc atgaactcct 480 tcaacacgca tgacgatact caaaagattg ttgagaagta ctccaactcc aacatcaaca 540 tccacacttt caaccagagc caatatccca ggattgttac tgaagacttc ttgccacttc 600 cgagcaaagg gaagtcagga aaggatggct ggtatccccc aggccatggt gatgttttcc 660 cctctttgaa caacagtgga aaacttgata ccttactgtc gcagggcaag gagtatgtct 720 tcgttgccaa ctcagacaac ttgggtgcta tagttgacat caagatattg aaccacctga 780 tcaacaacca gaatgaatac tgcatggagg ttactccgaa aacattggct gatgttaaag 840 gtggcaccct catctcatat gaaggcaggg tccagctctt ggagattgcc caagtccctg 900 atgagcatgt gaatgaattc aagtcaattg agaagttcaa gatattcaat accaacaacc 960 tgtgggtgaa cttgaaggct atcaagaggc ttgttgaagc tgatgcactt aagatggaga 1020 tcattcccaa ccctaaggaa gttgatggcg tgaaagtcct tcagctagaa actgcagctg 1080 gggcagcgat ccggttcttc gagaaagcaa tcggcatcaa cggtccccgc tcaaggtttc 1140 tgcccgtgaa ggctacatca gatttgttgc ttgtgcagtc tgacctctat accttggtcg 1200 atggttatgt catccgcaac ccagctagag tgaagccttc aaacccttcc attgagcttg 1260 gtcctgagtt caagaaggtc gccagtttcc tggcccggtt caagtcaatc cccagcatcg 1320 ttgagctcga cagcttgaag gtctctggtg atgtcacgtt tggctctggc gtcgtactca 1380 agggcaacgt gaccatcgct gccaagtctg gagtcaagct ggagatccca gacggagctg 1440 tgcttgagaa caaggacatc aacggcccgg aggatctttg agcgacgctc actcgccacc 1500 gccagacgca tcctggaagc cttccagttc tccttccctg agttaacaac agtcttgtaa 1560 ttttcgtgtg cattctgccg tggggtcgtc ctgtgggagc ccggttacag aataattgta 1620 atcccctctg tccatctgca cttctgttct tcctgggtgg taccagggac gtaaagttct 1680 tttggttaaa aaaaaaa 1697 3 2174 DNA Lolium perenne 3 gctgtctccg gcggcgtacc tactctaact cgggcgacac gcacgcagat cccaacggtc 60 cggtctacta tggcggatgg taccacctct tctaccagca caacccctat ggcgactcgt 120 ggggaaacgt atcttgggga catgccgtgt ccaaggacct ggtgaactgg cgtcacctcc 180 cggtcgcctt ggtgcccgat cagtggtacg acatcaacgg cgtcctgacg ggctctatca 240 cagtgctccc agacgggcgt gtcatcctgc tatacacggg gaacaccgac accttttcgc 300 aggtccagtg cctcgcagtg cccgccgacc catctgaccc gctcctccgt agctggatca 360 agcaccctgc caaccccatc ctcttcccgc cacctgggat cgggctcaag gacttccgtg 420 acccgctcac ggcctggttc gaacattccg acaacacgtg gcgcaccatc atcggatcca 480 aggatgacga cggccacgcc ggcatcgtcc ttagctacaa gaccaccgac tttgtgaatt 540 atgagctcat gccagggaac atgcatcgtg gccccgacgg caccggcatg tacgagtgcc 600 ttgacatcta ccctgtgggc ggcaactcat ccgagatgtt gggtggcgac tcctcgccgg 660 aggtgttgtt cgtgctcaag gagagcgcca acgacgagtg gcacgactac tacgcgcttg 720 ggtggttcga cgccaccgcc aacacgtgga cgccacagga ccccgaggcg gaccttggga 780 tcggcctcag gtacgactgg ggcaagtact acgcatccaa gtccttctac gacccgatca 840 agaaccggcg tgtcgtttgg gctttcgtcg gcgagaccga ctctgagcag gccgacaaag 900 ccaagggatg ggcgtccctc atgtcgattc cgaggatggt ggagcttgac aagaagaccc 960 ggacgaacct catccaatgg ccagtggagg agatcgagac ccttcgcagg aacgtcacag 1020 acctcggtgg catcaccgtt gaagccggct ccgtcattca ccttcccctc caacaaggcg 1080 ggcagcttga catcgaggcc tccttccgcc tcaactcttc ggacatcgat gcactcaacg 1140 aggccgacgt tggcttcaac tgcagtagca gcgctggggc agccgtgcgt ggtgcgctcg 1200 gcccctttgg cctcctcgtc ttcgccgacg gtcgccacga acagacggca gcgtacttct 1260 acgtgtccaa gggcctcgac ggcagcctcc tgacgcacta ctgccacgac gagtcacggt 1320 cgacgcgagc aaaggacgtc gtgagccggg tggttggcgg cactgtgcca gtgcttgacg 1380 gtgaaacctt ttcagtgagg gtgctagtgg accactccat cgtgcagagc ttcgtgatgg 1440 gtgggaggac cacggtgaca tcgcgggcat acccgacgga ggccatctac gccgcggcag 1500 gggtgtacct gttcaacaac gcaacgagcg ccaccatcac cgccgaaggg ctcgtcgtgt 1560 acgagatggc ctcggccgag agtcaggcct tcttggctga cgacatgtag atgaaaacta 1620 gtgaagaaca tgtcaatggc gatcgtcaag cttgctggat ggggatcgtc aggtaaggag 1680 agcaggtcac agagatcttc attcgcaagt tcgcgggcat gttgtagcta gggtggtgcc 1740 attgcatgct gtggaggggc tgacggctct ctttggactg gattgcgatc tggccaagac 1800 ggtagatcga ggaagccctc gtcgcccatg gctgggcaaa gcagtttgga ccagaaggtg 1860 ttggttcatg tcgttgcacc tgatgacacg atggtgccca acgaggcatc ctgacttcca 1920 catcgtctct gcgcatgtca tgctccttac tatctacctc tccccttctg ttagttttgt 1980 tggtctcgtt cgctgtcgtc ctacctgatg tagctccaat ctttgttgcc ggtgcttttt 2040 tgtcccagtt gttcatccgt atctcgccaa ggtacggtta gctatattgt ttcaaacatg 2100 cttcgagctt gtaatgttta tattttttgc tggaaccgga gatgcctcaa cgatacagat 2160 atacaaaaaa aaaa 2174 4 2478 DNA Lolium perenne 4 atggagtcca gcgccgtcgt cgtcccaggc acaacggcgc cactgctccc gtacgactcc 60 cgtgaaaacc agagtagcgg cggcggtgtg tggtggcgcg cgtgcgcggc ctcggccgtg 120 gtgctgctgg tcgtcgtcgg cttcttcgct ggtggcaggg tggatttggg tcaggccggc 180 gaggtgtctg cgacttcttc tgttccggcg gcaatgatgg agatcccgag gagcaggggc 240 aagaatttcg gcgtgtcgga gaaggccgac ggcgggttcc cgtggagcaa cgccatgctg 300 cagtggcagc acaccgggtt ccatttccag ccactgaagc actacatgaa cgatcccaac 360 ggtccggtct actatggcgg atggtaccac ctcttctacc agcacaaccc ctatggcgac 420 tcgtggggaa acgtatcttg gggacatgcc gtgtccaagg acctggtgaa ctggcgtcac 480 ctcccggtcg ccttggtgcc cgatcagtgg tacgacatca acggcgtcct gacgggctct 540 atcacagtgc tcccagacgg gcgtgtcatc ctgctataca cggggaacac cgacaccttt 600 tcgcaggtcc agtgcctcgc agtgcccgcc gacccatctg acccgctcct ccgtagctgg 660 atcaagcacc ctgccaaccc catcctcttc ccgccacctg ggatcgggct caaggacttc 720 cgtgacccgc tcacggcctg gttcgaacat tccgacaaca cgtggcgcac catcatcgga 780 tccaaggatg acgacggcca cgccggcatc gtccttagct acaagaccac cgactttgtg 840 aattatgagc tcatgccagg gaacatgcat cgtggccccg acggcaccgg catgtacgag 900 tgccttgaca tctaccctgt gggcggcaac tcatccgaga tgttgggtgg cgactcctcg 960 ccggaggtgt tgttcgtgct caaggagagc gccaacgacg agtggcacga ctactacgcg 1020 cttgggtggt tcgacgccac cgccaacacg tggacgccac aggaccccga ggcggacctt 1080 gggatcggcc tcaggtacga ctggggcaag tactacgcat ccaagtcctt ctacgacccg 1140 atcaagaacc ggcgtgtcgt ttgggctttc gtcggcgaga ccgactctga gcaggccgac 1200 aaagccaagg gatgggcgtc cctcatgtcg attccgagga tggtggagct tgacaagaag 1260 acccggacga acctcatcca atggccagtg gaggagatcg agacccttcg caggaacgtc 1320 acagacctcg gtggcatcac cgttgaagcc ggctccgtca ttcaccttcc cctccaacaa 1380 ggcgggcagc ttgacatcga ggcctccttc cgcctcaact cttcggacat cgatgcactc 1440 aacgaggccg acgttggctt caactgcagt agcagcgctg gggcagccgt gcgtggtgcg 1500 ctcggcccct ttggcctcct cgtcttcgcc gacggtcgcc acgaacagac ggcagcgtac 1560 ttctacgtgt ccaagggcct cgacggcagc ctcctgacgc actactgcca cgacgagtca 1620 cggtcgacgc gagcaaagga cgtcgtgagc cgggtggttg gcggcactgt gccagtgctt 1680 gacggtgaaa ccttttcagt gagggtgcta gtggaccact ccatcgtgca gagcttcgtg 1740 atgggtggga ggaccacggt gacatcgcgg gcatacccga cggaggccat ctacgccgcg 1800 gcaggggtgt acctgttcaa caacgcaacg agcgccacca tcaccgccga agggctcgtc 1860 gtgtacgaga tggcctcggc cgagagtcag gccttcttgg ctgacgacat gtagatgaaa 1920 actagtgaag aacatgtcaa tggcgatcgt caagcttgct ggatggggat cgtcaggtaa 1980 ggagagcagg tcacagagat cttcattcgc aagttcgcgg gcatgttgta gctagggtgg 2040 tgccattgca tgctgtggag gggctgacgg ctctctttgg actggattgc gatctggcca 2100 agacggtaga tcgaggaagc cctcgtcgcc catggctggg caaagcagtc tggaccagaa 2160 ggtgttggtt catgtcgttg cacctgatga cacgatggtg cccaacgagg catcctgact 2220 tccacatcgt ctctgcgcat gtcatgctcc ttactatcta cctctcccct tctgttagtt 2280 ttgttggtct cgttcgctgt cgtcctacct gatgtagctc caatctttgt tgccggtgct 2340 tttttgtccc agttgttcat ccgtatctcg ccaaggtacg gttagctata ttgtttcaaa 2400 catgcttcga gcttgtaatg tttatatttt ttgctggaac cggagatgcc tcaacgatac 2460 agatatacaa aaaaaaaa 2478 5 2118 DNA Lolium perenne 5 gcggcgcggc tctcaagggg ccagcctacc cattccccgc ctctccggcc accagcgggg 60 agcgctgccc gacgaagcgt ggtgggtggg ccgccccaca ttgagttaga aacaaggcac 120 taatggggat tgcggaggtg gctctccaca ccatgccggg ggcatttgcc agccactccc 180 cggcatccag tttacccctc aggactgaca cgaggagttt gaggaagagg ggcaccaatt 240 cgttttacag gacacttgga ggtccaccaa agttccctga gttgcggccg gttgagtgcc 300 agtgccagag gattgatgac cttgcggggg tcatcgaagc tgggaacggg acatgggcca 360 ccgacatggt gaacaaggcc agccaggttc ttggtgatgt cgctgtgcct ggtcaggctt 420 taggtggcaa tgcaagtctg agtggagatc ctgagaaggt tctgcctagg aggcggaact 480 tgtcatcggt tgaggatgaa gcttgggacc ttttgaggga atctgttgtt aattattgtg 540 gtagtccagt tggaacaatt gctgccaatg atccaaatga cagtaatcca gcaaattatg 600 atcaggtgtt tattcgggac tttataccgt ctggcattgc ttttctattg aagggggagt 660 atgaaattgt acgcaatttc attctacaca cccttcagct tcagagctgg gagaagacaa 720 tggactgcca tagtccaggt caaggcttaa tgcctgccag cttcaaggtg cggactattc 780 cacttgacgg cgatgagaat gccactgagg aggtattgga tcctgatttc ggggaagctg 840 caatagggcg tgtggcacct gttgattcag gtctatggtg gatcatattg ctcagggcat 900 atggaaaatg ttcgggtgat ttgtcagttc aagagagaat tgatgtccag actggcataa 960 aaatgattct gaagctttgt ttagctgacg ggtttgacat gttccctaca ttactggtaa 1020 ctgatggttc atgcatgatt gatcgtcgaa tgggaatcca cggacatcca cttgaaattc 1080 aggcactgtt ctattcagct ctcttgagtg cacgtgagat gttgactcct gaagatggat 1140 cagctgactt aatccgtgcc ctaaataaca ggcttgtcgc gctgtccttt catatcaggg 1200 agtactattg ggtcgacatg caaaaactga atgagatata tcgatataaa actgaagaat 1260 attcttatga tgctgtcaac aagttcaaca tatatcctga tcaggtttct ccttggcttg 1320 ttgaatggat acctcctaaa gggggttact ttattggaaa cctgcagcct gcacatatgg 1380 acttccggtt cttttctttg gggaatttat ggtcaattgt aagcagcttg gcaacaaccc 1440 aacaatcaca tgctattttg gatctgattg aatcgaaatg gtctgattta gtggcagaga 1500 tgccactgaa gatatgttat cctgctcttg agaatctgga atggaaaatc attactggaa 1560 gtgaccctaa aaacacgcct tggtcatacc ataatggagg atcctggcca acattattgt 1620 ggcagctcac agtggcatct ctcaagatga acagaccaga gattgctgca aaagctgtgg 1680 agatagctga gcggcgcatt gctacagaca aatggcctga atactatgac acgaagcgag 1740 cacgcttcat agggaaacag tctcggcttt accagacatg gtctattgct gggtaccttg 1800 tagcgaagca actgctggac aaacctgatg ctgctcgaat actctggaac gacgaggaca 1860 cggaaattct taatgctttt agcacaaaca ggaaacgtgg caagaaagtg ttgaagaaga 1920 catacattgt gtgagttctc agcactgtta agttatagga tgtctcttct gtacatactt 1980 acaaaaggtc gtgcttttga tggaggaatg cccgtgttgg atgttgttgt aatggatgca 2040 tctggccttg caagaaatca cttgcttgag cattcctcaa ttatttactt gccatcactt 2100 tttgcactaa aaaaaaaa 2118 6 1942 DNA Festuca arundinacea 6 gaccccttcc gcgccgcgct cgcgcctgcg tcgccgccgc tcgaggcgcc cccccttgat 60 gagctcccca ccgccccgtc gcactccgag ccagcgtctg cggccgccgc ggcgcccgag 120 caggatccgg tggatttgca gcacgaggag ttggacggcc tcaaggccgg ggtggaggcg 180 gtgaggagca gggaggagtc gccgcaggag aaggaagcgt ggtggctgct caaccgtgcg 240 gttgtgaatt actgcggcag cgcggtgggg acggtggccg cgaacgaccc gtccacggcc 300 aaccacatgc tcaattacga ccaggtgttc atcagggact ttgtgccgtc tgccatcgcg 360 ttcctcctca agggtgagag cgacatcgtg aagaacttct tgctgcacac cttgcagctc 420 cagagttggg agaagacagt tgattgctat agccctggtc aggggttgat gcctgctagt 480 tttaaagtca gatctgtgcc tctagatgga aacaatgaag catttgaaga ggttcttgac 540 cctgactttg gagaatcggc tattgggcgt gtagcacctg ttgactctgg gctttggtgg 600 ataattctat tgagggcata tggtaaaatt actggagact atgcactaca agaaagggtt 660 gatgtgcaga caggcatcag actaatcctg aatttgtgct tgtctgacgg atttgacatg 720 tttcctacat tgttagtcac tgatggatca tgcatgattg atcggaggat gggaatccat 780 gggcatcctc ttgagatcca ggctctgttt tattctgctt tgcgatgtgc ccgtgaaatg 840 gtcaatatag atgatgggtc taagaacttg atccgtgtta tcaacaacag gctcagtgct 900 ctgtcatttc acataagaga gtactattgg gtggacatga agaagataaa tgagatttat 960 cgctacaaga ctgaggagta ctcacatgat gctatcaata agttcaacat ctacccagag 1020 caaatcccat cttggcttgc agactggatt cctgagaaag gtggctatct tataggaaac 1080 ctacaaccag ctcacatgga tttcaggttc ttttctctag gaaatctctg ggctattgtt 1140 tcctctttag ccactccaaa gcaagcagag ggtatcttga acctcattga gaccaaatgg 1200 gatgatatag ttgcaaatat gcctctcaag atatgctacc ctgctctgga gtatgaggaa 1260 tggcgtatta tcaccggttg tgaccccaaa aacacgccct ggtcgtatca taatggtgga 1320 tcttggccta cattgctatg gcagttcacc ctagcctgta tcaagatggg taggcctgac 1380 ctggcaagga gggctgttga ggccgtggag aagaggctct cggatgacaa gtggccagaa 1440 tattatgaca ccaggaatgg aaggtttatt ggaaaacagt cgaggctata ccaaacctgg 1500 acaattgcag ggtttcttag ctcaaaattg cttttggact gtccagagat ggcatcaata 1560 ttaatatgtg acgaagatct cgatctacta gaagggtgtg cttgtggcgc gaacaagagt 1620 gctcgcgtga aatgctcccg tcgtgcagcc aggtctcaag tccttgtgta gttccatact 1680 tttgcttgac agccaagacc tgcagtgctc ctttcgagtc acagaagttg gcacttgtta 1740 cctcaccagg gtgaccacct cctgtgccgg ttattttggc gagtttgtgg ccccttaatc 1800 tattgatacg agagtatact ttgttgtata gatttcaaca tgtgtacacg aagccaatta 1860 actcaagttg attggcagtt ttataaacag atagcatgta aatattacca cttgtaatca 1920 atttattccg agaaaaaaaa aa 1942 7 2250 DNA Festuca arundinacea 7 gaaagcggtt cgagcctgtg caaacccagc tcgaaatccc tcaattccac gaatccgatc 60 gaagcctttt cttcctccgc aatccaaatc tcgcgagaca agcgggcgct gcaatggcgg 120 ccgccgccat ctcccacctc cgacggggca cgcagcggca cgcgctcctg tacctctcgc 180 gccgccactt ctctaactcc cccctcaccg ccgccgcccc cctcgccgcc gccgcccgcc 240 gccttctctc cacgacagtg gaatccggca cgtcgtcggc ggcgggaagc tacaagcccc 300 cgcccctcga tcccttccgc gccgccctcg ccccagcgtc gccgccgctc gagtcgcctc 360 cccttgatga gctccccacc gccccgtcgc actccgagcc agcgtctgcg gccgccgcgg 420 cgcccgagca ggatccggtg gatttgcagc acgaggagtt ggacggcctc aaggccgggg 480 tggaggcggt gaggagcagg gaggagtcgc cgcaggagaa ggaagcgtgg tggctgctca 540 accgtgcggt tgtgaattac tgcggcagcg cggtggggac ggtggccgcg aacgacccgt 600 ccacggccaa ccacatgctc aattacgacc aggtgttcat cagggacttt gtgccgtctg 660 ccatcgcgtt cctcctcaag ggtgagagcg acatcgtgaa gaacttcttg ctgcacacct 720 tgcagctcca gagttgggag aagacagttg attgctatag ccctggtcag gggttgatgc 780 ctgctagttt taaagtcaga tctgtgcctc tagatggaaa caatgaagca tttgaagagg 840 ttcttgaccc tgactttgga gaatcggcta ttgggcgtgt agcacctgtt gactctgggc 900 tttggtggat aattctattg agggcatatg gtaaaattac tggagactat gcactacaag 960 aaagggttga tgtgcagaca ggcatcagac taatcctgaa tttgtgcttg tctgacggat 1020 ttgacatgtt tcctacattg ttagtcactg atggatcatg catgattgat cggaggatgg 1080 gaatccatgg gcatcctctt gagatccagg ctctgtttta ttctgctttg cgatgtgccc 1140 gtgaaatggt caatatagat gatgggtcta agaacttgat ccgtgttatc aacaacaggc 1200 tcagtgctct gtcatttcac ataagagagt actattgggt ggacatgaag aagataaatg 1260 agatttatcg ctacaagact gaggagtact cacatgatgc tatcaataag ttcaacatct 1320 acccagagca aatcccatct tggcttgcag actggattcc tgagaaaggt ggctatctta 1380 taggaaacct acaaccagct cacatggatt tcaggttctt ttctctagga aatctctggg 1440 ctattgtttc ctctttagcc actccaaagc aagcagaggg tatcttgaac ctcattgaga 1500 ccaaatggga tgatatagtt gcaaatatgc ctctcaagat atgctaccct gctctggagt 1560 atgaggaatg gcgtattatc accggttgtg accccaaaaa cacgccctgg tcgtatcata 1620 atggtggatc ttggcctaca ttgctatggc agttcaccct agcctgtatc aagatgggta 1680 ggcctgacct ggcaaggagg gctgttgagg ccgtggagaa gaggctctcg gatgacaagt 1740 ggccagaata ttatgacacc aggaatggaa ggtttattgg aaaacagtcg aggctatacc 1800 aaacctggac aattgcaggg tttcttagct caaaattgct tttggactgt ccagagatgg 1860 catcaatatt aatatgtgac gaagatctcg atctactaga agggtgtgct tgtggcgcga 1920 acaagagtgc tcgcgtgaaa tgctcccgtc gtgcagccag gtctcaagtc cttgtgtagt 1980 tccatacttt tgcttgacag ccaagacctg cagtgctcct ttcgagtcac agaagttggc 2040 acttgttacc tcaccagggt gaccacctcc tgtgccggtt attttggcga gtttgtggcc 2100 ccttaatcta ttgatacgag agtatacttt gttgtataga tttcaacatg tgtacacgaa 2160 gccaattaac tcaagttgat tggcagtttt ataaacagat agcatgtaaa tattaccact 2220 tgtaatcaat ttattccgag aaaaaaaaaa 2250 8 973 DNA Lolium perenne 8 atttgcttga aaagagaaag ctaaatgaaa tctatagata caaaacagaa gaatattctt 60 atgatgccgt caacaagttt aacatatatc ccgatcagat tcctccctgg ctagttgaat 120 ggatccctcc gaaagggggt tatttcatcg gaaacctgca accagctcac atggatttcc 180 gattcttttc tctagggaac ttgtggtcta tagtaagcag tttggcaaca gctgatcaat 240 ctcatgctat tctggatcta gtggaagcaa aatggtctga tctagtggca gagatgccaa 300 tgaagatatg ttatcctgct cttgaggatc aagagtggaa atttattact gggagcgacc 360 ctaaaaatac accttggtca taccataatg gaggttcctg gccaacattg ttgtggcagc 420 tcacggtggc atgcatcaag atgaaccggc ccgagatcgc cgcaagagct gtggaggtgg 480 ctgaaagccg tatttccatg gataaatggc ccgaatacta cgataccaag cgtgggcggt 540 tcatcggtaa gcaggcccgg ttattccaaa cttggtccat tgccggcttt cttgtggcca 600 aactgctgct agaaaatccc gaaaaatcta gaatactctg gaacaacgaa gatgaggaaa 660 ttcttaatgc tttgagtctg atgactggcc catccagtcc gaagaggaag cgtggtagga 720 agacctatat tgtgtaagtc caacagcagt tctaacctct agggtttcat gggtgttgca 780 tttagttatg taagaatcgt ccacatatac cgttagagat atatttggta taggtatatt 840 aggtagtcta ggatttgtaa cctctaccta ccatatctct aggagagcta tcttagcctc 900 caagtcttgt accactatat atactcgccc gagaggctca atacaacatc aatcatattc 960 cgcaaaaaaa aaa 973 9 2019 DNA Lolium perenne 9 gaaaaccgtt cgcctttcgc aactcgctcc aggcgtctgc gcgcgcatgg cgatcgcggc 60 agcggccgcg ctgctgccgc tgcacctcgg atgctccgac gcggctcccc ggcggcccgg 120 taactccctc agagcccatc tgcggaaggg cgggatcagg ggcaggcggc ggagccctcc 180 gtgcgccgtc aactcgctgc atcccagcgg caaccccaaa actcccggcg gcggcgacgt 240 tggcggagcg tggggcttga acggcggcgc caccgccaag cccgatcacg cgccgccgag 300 ccagaggagg cgcgcgccgc gcgacgtgga ggaggaggcg tgggcgctcc tccgggagtc 360 ggtggtgagc tactgcggca gccccgtggg caccatcgcc gcgtgcgacc ccaatgacgc 420 cagcccgctc aactacgacc aggtgttcat ccgggacttc gtgccctccg gcgtcgcctt 480 cctcctcaag ggggaacacg aaatcgtccg caacttcatc ctccacacgc tccagctcca 540 gagctgggag aaaacaattg actgtcatag cccgggccaa gggttaatgc cggctagttt 600 caaggtgcgt gttgttccac ttgatggtgg cgacgatggt gcgactgagg aagtcttgga 660 tcctgacttt ggggaggctg ctataggccg tgtggcacca gttgattcag gtctgtggtg 720 gatcatacta ctgagggcgt atggaaaatg ttcaggggac ctctcattcc acgagagagt 780 ggatgtccag actggaataa aactgatctt gaagctctgc ttagctgatg ggtttgacat 840 gttccccacg ttgctagtca ctgatggctc ctgcatgatg gatcggcgaa tgggtatcca 900 tggacacccg ctggaaattc aggctctgtt ctattcagcc ctcttgtctg cacgtgagat 960 gcttacccca gaagatggat cggctgactt gatccgtgcc ctaaatagca ggcttatggc 1020 actctctttc catattaggg agtattattg gcttgaaaag agaaagctaa atgaaatcta 1080 tagatacaaa acagaagaat attcctatga tgccgtcaac aagtttaaca tatatcccga 1140 tcagattcct ccctggctag ttgaatggat ccctccgaaa gggggttatt tcatcggaaa 1200 cctgcaacca gctcacatgg atttccgatt cttttctcta gggaacttgt ggtctatagt 1260 aagcagtttg gcaacagctg atcaatctca tgctattctg gatctagtgg aagcaaaatg 1320 gtctgatcta gtggcagaga tgccaatgaa gatatgttat cctgctcttg aggatcaaga 1380 gtggaaattt attactggga gcgaccctaa aaatacacct tggtcatacc ataatggagg 1440 ttcctggcca acattgttgt ggcagctcac ggtggcatgc atcaagatga accggcccga 1500 gatcgccgca agagctgtgg aggtggctga aagccgtatt tccatggata aatggcccga 1560 atactacgat accaagcgtg ggcggttcat cggtaagcag gcccggttat tccaaacttg 1620 gtccattgcc ggctttcttg tggccaaact gctgctagaa aatcccgaaa aatctagaat 1680 actctggaac aacgaagatg aggaaattct taatgctttg agtctgatga ctggcccatc 1740 cagtccgaag aggaagcgtg gtaggaagac ctatattgtg taagtccaac agcagttcta 1800 acctctaggg tttcatgggt gttgcattta gttatgtaag aatcgtccac atataccgtt 1860 agagatatat ttggtatagg tatattaggt agtctaggat ttgtaacctc tacctaccat 1920 atctctagga gagctatctt agcctccaag tcttgtacca ctatatatac tcgcccgaga 1980 ggctcaatac aacatcaatc atattccgca aaaaaaaaa 2019 10 2457 DNA Lolium perenne 10 gctaccgccc aacctaaaca aaaccgtacc gaaccctgca attttgccat caacccctcg 60 ccgaccacat atttgcaaaa gcttaccttc aactgtacta tcccttttac gccgagcccc 120 ctacgaggtt tccgcatctc gcttctgagt ccttcgccgg agtttccata tgaatggtca 180 aaccacgatg gggctcgcag cagccgccgc cgcagccgtg aggccgtgcc gccgccgcct 240 cctctcctcc gcctcagcgg cggcggcggc gaaggcctcc gcgacgccgc tcttcccgag 300 atgctcccac ccgcagcacc agcagcacag ccgccgcatc ccattcctcg tctcggcggc 360 gtcgcacacg tcgcagtccg acccgagcac cacccccacc cccgtcacct ccgatccccg 420 ctccgccgtc gccgggaacc tccccttctt cgaccgcgtg ctcttcccgg gctcgttccc 480 cctcgagacc ccgcctgtcg aggagccggc gccggcgccg ccggccgatg aagcgcaggc 540 gtccgcttcg cccgtgagag aggagtcgga tacggagagg gaggcgtgga ggctgctgag 600 gagggcggtg gtgagctact gcggtgaccc ggtgggcacg gtggcggcgg aggacccgga 660 gtgcacggag atgctcaact acgaccaggt cttcatcaga gactttgtgc cttccgccct 720 cgccttcctc atgcgcgggg agaccgagat cgtccgcaat ttcctcctcc acaccctgca 780 gctgcagagc tgggagaaaa ctgttgactg ttacagccct gggcaaggct tgatgccagc 840 tagttttaag ataaagaccg ttccacttga tgaaaacaac gaagcattcg aggaggttct 900 ggatcctgac tttggtgaat cagctattgg ccgtgtagct ccagttgatt ctggactttg 960 gtggattatc ttactaagag cgtactgcaa gtttacaggc gactattcat tgcaagaaag 1020 agtggatgtg caaaccggga ttaaactgat cttgagtttg tgtttgactg atgggttcga 1080 catgtttccc acactactgg tcacagacgg atcatgcatg atagacagga ggatgggaat 1140 acatggacat cctcttgaga ttcaagcttt gttctattct gctctaagat gctcaaggga 1200 aatgattgtt atgaacgatg gctcaaaaca cctcctccaa gccatcaaca acaggctcag 1260 tgcgttgtct tttcacatta gggaatacta ctgggtcgat atgaagaaga taaatgagat 1320 ctacagatac aagacagaag aatactcaca tgatgcgacc aacaaattca acatttatcc 1380 cgagcaaatc ccttcctggc ttgttgattg ggttcctgag aaagggggtt atcttattgg 1440 aaatctgcag ccagctcaca tggattttag gttcttctcc cttggcaacc tttgggccat 1500 atcttcatct ctaactactc caacccaagc cgaaggaata cttagcctta ttgaggagaa 1560 atgggacgat cttgtggcaa atatgccact caagatatgt taccctgcaa tggaagatga 1620 tgaatggcgc attgttactg gcagtgaccc taagaacacc ccgtggtcat atcataatgg 1680 tggatcttgg ccaaccctgt tgtggcagtt tacactggct tgcatcaaaa tgggaagacc 1740 agagttggcc cgaagggcca ttgcagtggc tgaggaaaag ctctcagctg acaagtggcc 1800 ggaatactat gacacccgat ctggaagatt cgttgggaag caatcacggt catatcagac 1860 atggactatt gctggttttc tgacctcgaa gatattgctg gaaaacccgg agctggcttc 1920 tatcctgacc tgtgatgagg atcttgagct ccttgaaggc tgtgcttgct gcctctcaaa 1980 gaggacgagg tgctctcgtc gtgtgaccaa atcagatatc atcgggtaaa acagcagagc 2040 cccttttatt cttcatgctc tgcagaccat gtatactatc gactgagaat taactgaggc 2100 ggacacactg tagctgtgta cattataggt ttaagttaga tatcaatcca ttcatttcct 2160 caatgtgcgc tcattctttt tctctgagct gccattgatg ggaacaaccc tgggtgatac 2220 cggtggtcaa cgggagcatt accaatttat gttggatctc tcatgtacac acacaaaaaa 2280 aggaattatt cttgtatttg gtaaccagtt gctcctgatt cgggagtgct gtgaagccct 2340 aaccattgta tctatgtcag tatttgagtt gtatgttgca ttatttgcaa cgtaaactga 2400 gactttgtat cctatccttg ttatgaataa cgatactgtt gtcctccaaa aaaaaaa 2457 11 2143 DNA Lolium perenne 11 gcgctcctgc acgcaagctg cacgggccgc tcctgcacgc aagctgcacg gccaatccaa 60 tcaccgcagc accgttcgcc tttcgcaact cgctccaggc gtctgcgcgc gcatggcgat 120 cgcggcagcg gccgcgctgc tgccgctgca cctcggatgc tccgacgcgg ctccccggcg 180 gcccggtaac tccctcagag cccatctgcg gaagggcggg atcaggggca ggcggcggag 240 ccctccgtgc gccgtcaact cgctgcatcc cagcggcaac cccaaaactc ccggcggcgg 300 tgacgttggc ggagggaggg gcgtgaacgg cggcgccacc gccaagcccg accacgcgcc 360 gccgagccag aggaggcgcg cgccgcgcga cgtggaggag gaggcgtggg cgctcctccg 420 ggagtcggtg gtgagctact gcggcagccc cgtgggcacc atcgcggcct gcgaccccaa 480 cgacgccagc ccgctcaact acgaccaggt gttcatccgg gacttcgtgc cctccggcgt 540 cgccttcctc ctcaaggggg agcacgaaat cgtccgcaac ttcatcctcc acacgctcca 600 gctccagagc tgggagaaaa cgattgactg tcatagccca ggccaagggt taatgccggc 660 tagtttcaag gtgcgtgttg ttccacttga tggtggcgac gatggtgcga ctgaggaagt 720 cttggatcct gactttgggg aggctgcaat aggccgtgtg gcaccagttg attcaggttt 780 gtggtggatc atactactga gggcatatgg aaaatgttca ggggacctct cattccacga 840 gagagtggat gtccagactg gaataaaact gatcttgaag ctctgcttag cggatgggtt 900 cgacatgttc cccacgttgc tagtcactga tggctcctgc atgatggatc gtcgaatggg 960 tatccatgga cacccgctgg aaattcaggc tctgttctat tcagccctct tgtctgcacg 1020 tgagatgctt accccagaag atggatcggc tgacttgatc cgggccctaa atagcaggct 1080 tatggcactc tctttccata ttagggagta ttattggctt gaaaagagaa agctaaatga 1140 aatctataga tacaaaacag aagaatattc ttatgatgcc gtcaacaagt ttaacatata 1200 tcccgatcag attcctccct ggctagttga atggatccct ccgaaagggg gttatttcat 1260 cggaaacctg caaccagctc acatggattt ccgattcttt tctctaggga acttgtggtc 1320 tatagtaagc agtttggcaa cagctgatca atctcatgct attctggatc tagtggaagc 1380 aaaatggtcc gatctagtgg cagagatgcc aatgaagata tgttatcctg ctcttgagga 1440 tcaagagtgg aaatttatta ctgggagtga ccctaaaaat acaccttggt cataccataa 1500 tggaggttcc tggccaacat tgttgtggca gctcacggtg gcatgcatca agatgaaccg 1560 gcccgagatc gccgcaagag ctgtggaggt ggctgaaagc cgtatttcca cggataaatg 1620 gcccgaatac tacgatacca agcgtgggcg gttcatcggc aagcaggccc ggttattcca 1680 aacttggtcc attgccggct tccttgtggc caaactgctg ctagaaaatc ctgaaaaatc 1740 tagaatactc tggaacaacg aagatgagga aattcttaat gctttgagtc tgatgactgg 1800 cccatccagt ccgaagagga agcgtggtag gaagacctat attgtgtaag tccaacagca 1860 gttctaacct ctagggtttc atggatgttg catttagtta tgtaagaatc gtccacatac 1920 cactagattt gtacatatta aagtggatgt tgtagaggaa atgcccattt tgagatgcta 1980 tcatgctgtt ctagtgatct actgttagca aggctcaggg gaacggattg ttggctccgg 2040 agctactccg agcttcttaa ttctagaaag ttcatttcaa gtttttaaaa tgtcccacgt 2100 gttgtgggag taatctatga acttataaat gctaaaaaaa aaa 2143 12 2033 DNA Lolium perenne 12 atacacccag ctctggctcc caacatgccg tctcctcccc gcgcccgcgc actccacccc 60 cactccgccg cctcctcctc cgtctgcccg atccgacgcc gcgtccattc ccccacggag 120 cttaactgaa acagttgacc aaaacgtgga atcatgaaga gagtttcatc gcatgtctcc 180 attgcgtcag aggctgagat caatctcgac ctctcacgct tactaattga caagccaagg 240 tacacgttgg agcggaagag gtcatttgat gagcagtcat ggagcgagct cacccacacc 300 catcggcaaa acgacggctt tgatagtgta ctgcagtcac ctgcattccg cactgggttt 360 gactcaccgt tctcaatggg aacgcacttt ggtgagccaa gtgggccaca cccccttgtg 420 aatgaagcat gggaggcact caggaaatct gtagtgtatt ttcggggcca accagttggt 480 acaattgctg cggtagatca tgcatctgaa gaagtgctca attatgatca ggttttcgtc 540 cgggattttg ttcctagtgc attggctttt ctaatgaata acgagcccga aatagtgaag 600 aactttctgt tgaaaactct ccacttgcaa agctcagaaa aaatggtaga ccggttcaag 660 cttggagcag gagcaatgcc tgcaagtttc aaggtggacc gtaataaaag cagaaacact 720 gaaacattag ttgcagattt tggtgagagc gcgattggca gggtggcacc agtggattct 780 ggattttggt ggattattct gctccgggca tatacaaagt atactggaga tgctagtttg 840 tcagaatctc ctgattgtca gaagtgcatg aggctgatac tgaatctctg cttatctgag 900 ggattcgata ctttcccaac tctgctctgc acagatgggt gctcaatgat cgatcgtcga 960 atgggtatat atggttatcc tattgagatc caagctctgt tctacatggc attaagatgt 1020 gctctccaaa tgcttaagcc agatggtgaa gggaaggact tcattgagaa gatagggcaa 1080 cggctgcatg cattaaccta ccacatgaga aactacttct ggctggactt cccacatcta 1140 aacaatatct atagatacaa aacagaggag tactcccaca ctgctgtgaa taaattcaat 1200 gtcatcccag attcaattcc tgattgggtg tttgatttca tgccatgccg aggaggctac 1260 tttcttggca acgtcagccc tgctatgatg gacttcaggt ggtttgctct tggaaactgt 1320 attgccatta tatcatctct ggctactcct gagcagtcat cagcaataat ggatctgatc 1380 gaggagaggt gggatgaatt agtgggcgag gtgcctctga agatttgcta tcctgcaatt 1440 gagaaccatg agtggagaat aattactggc tgcgacccca agaatacccg gtggagttat 1500 cataatgggg gatcatggcc agttcttctg tggctgctga ctgcagcctg tatcaagacc 1560 gggcggccac aaatggcgaa gcgcgccatc gagctctccg aggctaggct tctgaaggat 1620 ggctggcccg agtactatga tggcaagctg ggaaagttcg taggtaagca ggccaggaag 1680 ttccaaacat ggtccattgc aggctacctg gtagcccgga tgatgttgga ggacccgtca 1740 acgctcatga tgatctccat ggaggaagac cggcctgtga agccgacaat gaggcggtcg 1800 gcgtcgtgga atgcctgaaa ggctgggtgg ttgtttctta agatatttct tttacttcaa 1860 tggtctgttc ggcagaaaaa aggtccggac ttggttgtaa ttgaactctg tcagttagga 1920 cagaagtgta tacgtcagat gatcgatcct agaagctaca ctgcattttc ttactgtcaa 1980 acctttgatt ttgctatgaa accagcaagc gaagattctg cttaaaaaaa aaa 2033 13 1866 DNA Lolium perenne 13 agtagcaccg cccaggaacc caactccggc gagagagctc ctatatcagc cacctgcctc 60 tctccaacgt ctcttttggt cgcctcccac ttgtactcgc cgtcgtaccc acttcccgta 120 cgtttgcgcc gccgcttgtc ttttctatct cgccgggcgt gtattctcga cgcgcgtgca 180 gcagccacgg cgaccgcggc ggtgtgcgag atggaggcgc cggggggcgg agcggggccg 240 atgccgacca cgccgtcgca cgcgtccata gcggactcgg acgacttcga cctgtcgcgg 300 ctgctgaacc accgcccgcg gatcaacgtg gagcggcagc gctccttcga cgaccgctcg 360 ctcggcgacc tctacctctc cgccatggac agccgcggcg ggtacatgga cagctacgac 420 agcatgtact cgccgggcgg cgggctccgc tcgctcaccg gcacgccggc ctcctccacg 480 cgcctctcct tcgagcccca gctcctggtc gccgaggcct gggaggccct ccgccgctcg 540 ctcgtctgct tccgtgggga gcccctcggc accatcgccg ccgtcgacag ctcctccgac 600 gaagtcctca actacgacca ggtgttcgtg cgggattttg tgccgagcgc gctggcgttc 660 ctgatgaacg gggagccgga catcgtgaag aacttcctgc tgaagacgct gctgctgcag 720 gggtgggaga agcggatcga ccggttcaag ctcggggagg gcgccatgcc ggcgagcttc 780 aaggtgctca aggacccgaa gcgcggggtg gacaccctgg cggcggactt cggcgagagc 840 gccatcgggc gcgtggcgcc ggccgactcc gggttctggt ggatcatcct gctccgcgcc 900 tacaccaagt ccaccggcga cctcaccctc gccgagacgc ccgagtgcca gaagggcatc 960 cggctcatca tgaaccagtg cctcgccgag gggttcgaca ccttccccac cctcctctgc 1020 gccgacggct gctgcatgat cgaccgcagg atgggcgtgt acgggtaccc gatcgagatc 1080 caagccctct tcttcatgtc actgcggtgc gcgctgctgc tgctgaagcc ggcggtggaa 1140 gggaacagca gcagcaagga cgacgacatc atggagcgga tcgtgacgcg gctgcacgcg 1200 ctcagctacc acatgcgcag ctacttctgg ctcgacttcc agcagctcaa cgtcatctac 1260 cgcttcaaga cggaggagta ctcccacacc gccgtcaaca agttcaacgt catcccggag 1320 tccatcccgg actggctctt cgacttcatg ccctcccgcg gcggatactt cgtcggcaac 1380 gtcagccccg ccaggatgga cttccggtgg ttcgcgctgg gcaactgcgt cgccatcctc 1440 gcgtcgctcg ccacgccaga gcaggccggc gccatcatgg acctcatcga ggagcgctgg 1500 gaggacctca tcggcgagat gccgctcaag atctgctacc cgaccatcga gggacacgag 1560 tggcagaacg tcaccggatg cgaccccaag aacaccaggt ggagctacca caacggagga 1620 tcatggccag tgctgatctg gctcctgacg gcggcgtgca tcaagaccgg gcggctcaag 1680 atcgcgaggc gggcgatcga cctggcagag gcgaggctgg ggaaggacgg ctggccggag 1740 tactacgacg gcaagctcgg gcggtacgtg gggaagcagg cgaggaagca ccagacgtgg 1800 tccatcgcgg ggtacctggt ggccaagatg atgctggagg acccgtccca cctgggcatg 1860 atctcg 1866 14 2058 DNA Lolium perenne 14 gccatagatc tggccgcggc gagcaggcgg aggccggccg aggattcctg agcaggggac 60 ggaagcgaag agaagggcat ggagttcggg gcgccgggcg ggatgcggcg gtcggcgtcg 120 cacaactcgc tgtccggctc ggacgacttc gacctcacgc acctgctcaa caagccgcgg 180 atcaacgtcg agcgccagcg ctccttcgac gaccgctccc tcagcgacgt gtcctactcc 240 ggcggcggac acgccagggg cgctggcggg ggattcgacg gcatgtactc gccgggcggc 300 gggctgcgct cgctcgtcgg cacgccggcc tcgtccgcgc tccactcctt cgagccgcac 360 cccatcgtcg gggacgcgtg ggaggcgcta cgacgctcgc tcgtcttctt ccgcggccag 420 ccgctcggaa ccatcgccgc ctacgaccac gcctcagagg aggtgctcaa ctacgaccag 480 gtgttcgtgc gggatttcgt gcccagcgcc atggccttcc tcatgaacgg cgagccggag 540 atcgtcaaga acttcctgct caagaccgtg ctgctgcagg gctgggagaa gaaggtcgac 600 cgcttcaagc tcggcgaggg ggccatgccc gccagcttca aggtgctaca cgacgacaag 660 aagggcgtcg acacgctgca cgccgatttc ggggagagcg ccattggccg ggtcgcgcca 720 gtggactcgg gcttctggtg gatcatactg ctgcgggcct acaccaagtc cacgggggac 780 ttgaccctgg ccgagaagcc ggagtgccag aaggccatga ggctcatact cagcctctgc 840 ctctccgagg ggttcgacac cttccccaca ttgctgtgtg ctgatggatg ctgcatgata 900 gatcgaagga tgggtgtgta tggctacccc attgaaattc aatccctgtt cttcatggca 960 ctgaggtgtg ctcttctaat gcttaagcat gataatgaag ggaaagattt tgtggagcgg 1020 attgcaactc gtcttcatgc tttaagttat cacatgcgga gttacttttg gctggatttc 1080 cagcagctaa atgatattta tcgttacaag acggaagaat attctcacac agctgtcaac 1140 aagttcaatg tcattccaga ttctattccg gactggctgt ttgattttat gccttgtgaa 1200 ggtggttttt ttgttggcaa tgtcagtcct gcaaggatgg acttccgttg gtttgcactt 1260 ggtaacatga ttgccatagt atcatctctt gccacacctg agcaatctac ggctataatg 1320 gatctcattg aggagcggtg ggaagagcta attggtgaaa tgcctctgaa gatatgctat 1380 cctgccattg agaaccatga gtggcgaata gtgacggggt gtgacccaaa aaatacgaga 1440 tggagttacc acaatggagg atcttggcca gtacttctct ggctgctgac ggcagcaagc 1500 atcaaaactg gacggccgca aattgcaaga agagcaatcg acctagctga gaggaggctt 1560 ttgaaggatg gctggcctga gtattatgac gggaagctcg gaaaatatgt tggcaagcag 1620 gcaaggaaat ttcaaacttg gtctattgcc gggtatttgg tcgctaagat gctgcttgag 1680 gacccttcac atcttggaat gatagccctg gaggaagaca aggctatgaa gccagttttg 1740 agaaggtccg cctcatggac aaactaagat atcgacgaaa actcttaggg gagcaaagtc 1800 tggattgaaa acacgaattc tttgggcagc acttctctct gctcatcctt tctttgactt 1860 tcctaacgga aaggtttgtt ttcctctgga ttgtacaata tctcagctca tttcttgagt 1920 tggaaaagaa gcaattgtgg aaatgggcat tttgttttgt ttttcccttt caatcccgtg 1980 ttgtaagaag atacttccga ttcttgattg ggtcatcctg aagttatggg atccttttgg 2040 ttggtctcaa aaaaaaaa 2058 15 2167 DNA Lolium perenne 15 ggccaccacc aacaccaaaa acgcatcgac gcggcgcaga tacaaacgag gcagccgccc 60 ttcgccggcg aggcgatgga ttccgactac ggcgtgccgc gcgagctctc ggaggtgcag 120 aagaagcgga ccctctacca gcccgacctg ccgccctgcc tccagggcac tactgtgagg 180 gtggagtatg gtgatgtggc gatcgctgct gatcctgccg gcgctcatgt gatcagccac 240 gcgttcccac acacatacgg gcagccactg gctcatttcc tcaggaaggc ggctaatgtc 300 gctgatgcta aagtcatatc agagcaccct gccgtcaggg ttggcattgt attctgtgga 360 aggcagtccc caggaggcca caatgtcata tggggactcc atgatgctat caaagctcac 420 aacccgaata gcaaacttat tggtttcctc ggaggatccg atggtcttct tgcacagaaa 480 actttggaga tcacagatga agttctttct tcctacaaaa accaaggtgg ttatgatatg 540 cttggtagga ccaaggatca aatcagaaca acagagcaag tcaatggtgc aatggctagt 600 tgccaggctt tgaagttgga tgctctgata ataattggag gtgtcacgtc aaatacagat 660 gctgctcaac ttgctgagac ttttgccgag gcaaagtgtg caacaaaggt tgtaggtgtt 720 cctgtaactt tgaatgggga tctcaagaac caatttgttg agacaactgt tggttttgat 780 accatatgca aggtcaactc acaacttata agcaatatgt gcaccgatgc tctatctgct 840 gagaagtatt actatttcat ccgtatgatg ggacggaagg cttcccatgt ggcattggag 900 tgtgctcttc aatcgcatcc aaatatggtt atccttggtg aggaggttgc tgcatcaaaa 960 ctcacaattt ttgatatcac aaagcaaata tgtgatgcag ttcaggcgag agctgaaaaa 1020 gacaagaacc atggtgtcat acttattcct gagggccttg tggagagtat tcctgaatta 1080 tatgctctcc ttcaggaaat taatggcctt cacggtaaag gtgtttccat tgagaatatc 1140 tcttctcagc tttctccttg ggcatcagcg ctatttgagt ttttgcccca gtttattagg 1200 cagcagctgc ttctccgccc tgaatctgat gattcagctc aactttctca gattgaaact 1260 gaaaagcttc tagcccaatt ggttgaaacc gaaatgaaca aacgtttgaa ggaaggcacc 1320 tacaaaggaa agaagttcaa tgcaatctgt cacttttttg gctaccaagc taggggtgca 1380 atgccttcga agtttgactg cgattatgcc tatgttctgg gtcacgtgtc ttaccacatc 1440 ttggcagctg gtttgaacgg ttacatggct actgtgacaa atcttaagag tcccctgaac 1500 aagtggcgat gtggtgctgc tcctatttcg tccatgatga ccgtgaagcg atggtcacgt 1560 ggcccttcaa ccacacaaat cgggaagcca gctgtgcata tggctagtgt tgacttgaga 1620 ggaaaagcat atgagctgtt gaggcagaat tcatccagct gcttgttgga agacatctac 1680 agaaaccctg gaccactcca attcgaagga ccgggttctg attccaagcc tatttcactg 1740 tgcgttgagg atcaagacta catgggtagg atcaagaaat tgcaggagta cttggagaag 1800 gtgaagagca tagtgaagcc tgggtgctca caggatgttc tcaaggcggc gctgagtgcc 1860 atgtcttctg tgacagatac tctggctatc atgacttctt cttccactgg ccaggcccca 1920 ctctgagagt cgagttactc tgatctacat gtttccctat cctcttttgt tccatctgac 1980 ggttgggatt agagaacagt gatcttgtga tcccgtggtt cgttcttttc ctagtttgca 2040 gagagttttt gtcattcctg gctctgatag tgtaccgagg gtttgttgtt ggcgaggttg 2100 aactggaata atcgatcaaa ctgccggttg tgatctatta ataaactaaa ttttgataaa 2160 aaaaaaa 2167 16 2179 DNA Festuca arundinacea 16 ggccaccatc ccaccaacaa caaaaaaaac gcatcgacgc ggcgcagata caaacgaggc 60 aggcgacgag tgctaggcga tggattccga ctacggcgtg ccgcgcgagc tctcggaggt 120 gcagaagaag cggaccctct accagcccga gctgccgccc tgcctccagg gcactactgt 180 gagggtggag tatggtgatg tggcgatcgc tgctgatcct gctggcgctc atgtgatcag 240 ccacgctttc ccacacacgt acgggcagcc actggctcat ttcctcagga aggcggctaa 300 tgtcgctgat gctaaagtca tatcagagca ccctgccgtc agggttggca ttgtattctg 360 tggaaggcag tccccaggag gccacaatgt catatgggga ctccatgacg ctatcaaagc 420 tcacaactcg aatagcaaac ttattggttt cctcggagga tccgatggtc ttcttgcaca 480 gaaaactttg gagatcacag atgaagttct ttcttcctac aaaaaccaag gtggttatga 540 tatgcttggt aggactaagg atcaaatcag aacaacagag caagtcaatg gtgcaatggc 600 tagttgccag gatttgaagt tggatgctct gataataatt ggaggtgtca cgtcgaatac 660 agatgctgct cagcttgctg agacttttgc cgaggcaaag tgtgcaacaa aggttgtagg 720 tgttcctgta actttgaatg gagatctcaa gaaccaattc gttgagacaa ctgttggttt 780 tgataccata tgcaaggtga actcacaact tataagcaat atgtgcaccg acgctctatc 840 tgctgagaag tattactatt tcatccgtat gatgggacgg aaggcttccc atgtggcatt 900 ggagtgtgct cttcaatcgc atccaaatat ggttatcctt ggtgaggagg ttgctgcatc 960 aaaactcaca atttttgata tcacaaagca aatatgtgat gcagttcagg cgagagctga 1020 aaaagacaag aatcatggtg ttatacttat tcctgagggc cttgtggaga gtattcctga 1080 attatatgct ctccttcagg aaattaatgg ccttcacggt aaaggtgttt ccattgagaa 1140 tatctcttct cagctttctc cttgggcatc tgcgctattt gagtttttgc cccagtttat 1200 taggcatcag ctgcttctcc gccctgaatc tgatgactca gctcaacttt ctcagattga 1260 aactgaaaag cttctagccc aattggttga aaccgaaatg aacaaacgtt tgaaggaagg 1320 cacctacaaa ggaaagaagt tcaatgcaat ctgtcacttt tttggctacc aagcgagggg 1380 tgcaatgcct tcgaagtttg actgcgatta tgcctatgtt ctgggtcatg tgtcttacca 1440 catcttggca gctggtttga acggttacat ggctactgtg acaaatctta agagtcccct 1500 gaacaagtgg cgatgtggtg ctgctcctat ttcgtccatg atgactgtga agcgatggtc 1560 acgtggccct tcaaccacac aaatcgggaa gccagctatg catatggcta ctgtcgactt 1620 gagaggaaaa gcatatgagc tgttgaggca gaattcatcc agctacttgt tggaagacat 1680 ctacagaaac cctggaccac tccaatttga aggaccgggt gctgattcca agcctatttc 1740 gctgtgcgtt gaggatcaag actacatggg caggatcaag aaattgcagg agtacttgga 1800 gaaggtgaag agcatagtga agcctgggtg ctcacaggat gttctcaagg cggcgctgag 1860 tgccatgtct tctgtgacgg agactctggc tatcatgact tcatcttcca ctggccaggc 1920 cccactctga gagtcgagtt actcaagtgg gctaaaaatc tacgtttccc tatcctcttt 1980 tgttccatct gacggttggg attagagaac agtgatcttg tgatcctgta gtttgttctt 2040 ttcctagttt gcagagtttt tgtcatttct ggctctgata gtgttcgagg gtttgttgtt 2100 ggcgaggttg acctggaata atcgatcaaa ctgccggttg tgatctatta ataaactaaa 2160 ttttgataca aaaaaaaaa 2179 17 1961 DNA Lolium perenne 17 ctcacctcac ctgcctccgt ctctccgccc gaaagcgcat attcctccaa atctcacccc 60 gtcaccaccc tcgccggcgc atcgcatcgc atggcggccg ccgcggtggc cacctccaac 120 ggcgcctcgg ccaacgggcc gacgcccggg cgcctcgcgt ccgtgtacag cgaggtgcag 180 acgagccgca tcgcgcacgc gctgcccctc ccctccgtcc tccgctccca cttcacgctc 240 gccgacgggg ccgccagctc cgccacgggc aaccccgagg agatcgccaa gctcttcccc 300 aacctgtacg gccagccgtc cgcggccgtg gtgccctcgg cccagccggt cgccaccaag 360 ccgctcaaga tcggcgtcgt gctctccggc ggccaggcgc caggcggcca caatgtgatc 420 tgcggcatct ttgactacct gcaagagcgt gcgaagggca gcaccatgta cggattcaag 480 ggaggcccag ctggggtcat gaagggcaag tacgtcgagc tcaatgctga tttcgtctac 540 ccctacagga accagggtgg atttgatatg atctgcagtg gaagggacaa gattgaaaca 600 ccagagcagt tcaagcaagc tgaagacact gtcaccaaac ttgatttgga tggacttgtt 660 gtcattggtg gtgatgattc aaacactaac gcatgcctcc ttggtgaata cttcagggga 720 aggaacttga agactcgtgt tattggttgc cccaagacta ttgatggaga tctgaaatgc 780 aaggaggtcc caacaagctt tggatttgac actgcttgca agatatactc tgaaatgatt 840 ggcaatgtga tgactgatgc tcggtcaaca ggaaaatact atcactttgt gaggcttatg 900 ggccgagctg cttctcacat tacattagag tgtgctctgc aaacacaccc taacgtttca 960 ctcattggcg aagaggttgc tgagaagaag gaaacactca agcaagtcac agactacatt 1020 actgatgtta tctgcaaacg tgcagaactt ggttacaact atggagttat ccttatcccg 1080 gagggactta ttgatttcat tccagaggtt caaaagctca ttgcagaatt gaatgaaatt 1140 ttggcacatg atgttgttga cgaggcaggt gcatggaaaa gcaagcttca accagaatct 1200 aggcaactgt ttgacttctt gcccaacacc attcaggagc agcttttgct tgaaagagat 1260 ccacatggca atgttcaggt tgcgaaaatt gaaactgaga agatgcttat tgccatggtt 1320 gaaactgaat tggagaagag aagatctgca gggaagtact cagcacattt cagaggccag 1380 tctcacttct ttggatatga aggaagatgt ggtcttccta caaattttga ttctagctac 1440 tgctatgcat taggctatgg tgctggggct cttctccaat ttggaaagac aggacttatt 1500 tcgtcggttg gtaaccttgc tgctcctgtg gaagaatgga ctgtcggagg aactccattg 1560 acggcgttga tggatgtaga gaggagacat ggcaagttca agccagtgat caagaaggct 1620 atggtggaac ttgatgctgc gccattcaag aagtttgctt ccatgcggga tgaatgggcc 1680 atcaagaaca gatacatcag ccctggcccc atccagttca gcggccctgg aagcgatgcg 1740 tcgaaccaca ccttgatgct ggagcttggt gctcagacat gagatgctgt gttatagagt 1800 gcacctcttc tgtttttttt ctccctcctt acagttttga gagtggagac caaacctccc 1860 agtgggcagt ctccacattg tggaatgatt aataagagct attggagttt cctgagtgga 1920 tttcgtagca ataataactg attttagctg caaaaaaaaa a 1961 18 1959 DNA Lolium perenne 18 ctcacctcac ctgcctccgt ctctccgccc gaaagcgcat attcctccaa atctcacccc 60 gtcaccaccc tcgccggcgc atcgcatcgc atggcggccg ccgcggtggc cacctccaac 120 ggcgcctcgg ccaacgggcc gacgcccggg cgcctcgcgt ccgtgtacag cgaggtgcag 180 acgagccgca tcgcgcacgc gctgcccctc ccctccgtcc tccgctccca cttcacgctc 240 gccgacgggg ccgccagctc cgccacgggc aaccccgagg agatcgccaa gctcttcccc 300 aacctgtacg gccagccgtc cgcggccgtg gtgccctcgg cccagccggt cgccaccaag 360 ccgctcaaga tcggcgtcgt gctctccggc ggccaggcgc caggcggcca caatgtgatc 420 tgcggcatct ttgactacct gcaagagcgt gcgaagggca gcaccatgta cggattcaag 480 ggaggcccag ctggggtcat gaagggcaag tacgtcgagc tcaatgctga tttcgtctac 540 ccctacagga accagggtgg atttgatatg atctgcagtg gaagggacaa gattgaaaca 600 ccagagcagt tcaagcaagc tgaagacact gtcaccaaac ttgatttgga tggacttgtt 660 gtcattggtg gtgatgattc aaacactaac gcatgcctcc ttggtgaata cttcagggga 720 aggaacttga agactcgtgt tattggttgc cccaagacta ttgatggaga tctgaaatgc 780 aaggaggtcc caacaagctt tggatttgac actgcttgca agatatactc tgaaatgatt 840 ggcaatgtga tgactgatgc tcggtcaaca ggaaaatact atcactttgt gaggcttatg 900 ggccgagctg cttctcacat tacattagag tgtgctctgc aaacacaccc taacgtttca 960 ctcattggcg aagaggttgc tgagaagaag gaaacactca agcaagtcac agactacatt 1020 actgatgtta tctgcaaacg tgcagaactt ggttacaact atggagttat ccttatcccg 1080 gagggactta ttgatttcat tccagaggtt caaaagctca ttgcagaatt gaatgaaatt 1140 ttggcacatg atgttgttga cgaggcaggt gcatggaaaa gcaagcttca accagaatct 1200 aggcaactgt ttgacttctt gcccaacacc attcaggagc agcttttgct tgaaagagat 1260 ccacatggca atgttcaggt tgcgaaaatt gaaactgaga agatgcttat tgccatggtt 1320 gaaactgaat tggagaagag aagatctgca gggaagtact cagcacattt cagaggccag 1380 tctcacttct ttggatatga aggaagatgt ggtcttccta caaattttga ttctagctac 1440 tgctatgcat taggctatgg tgctggggct cttctccaat ttggaaagac aggacttatt 1500 tcgtcggttg gtaaccttgc tgctcctgtg gaagaatgga ctgtcggagg aactccattg 1560 acggcgttga tggatgtaga gaggagacat ggcaagttca agccagtgat caagaaggct 1620 atggtggaac ttgatgctgc gccattcaag aagtttgctt ccatgcggga tgaatgggcc 1680 atcaagaaca gatacatcag ccctggcccc atccagttca gcggccctgg aagcgatgcg 1740 tcgaaccaca ccttgatgct ggagcttggt gctcagacat gagatgctgt gttatagagt 1800 gcacctcttc tgtttttttt ctccctcctt acagttttga gagtggagac caaacctccc 1860 agtgggcagt ctccacattg tggaatgatt aataagagct attggagttt cctgagtgga 1920 tttcgtagca ataataactg attttagcta aaaaaaaaa 1959 19 1954 DNA Festuca arundinacea 19 gtgcctccgc ccctccgccc gaaagcatat tcctccaaat ctcgcgatac ccccgtcacc 60 acctcgccgg cgcatcgcat cgcatggcgg ccgcggcggt ggccacctcc aacggggcct 120 cggcgaacgg gccgacgccc gggcgcctcg cgtccgtgta cagcgaggtg cagacgagcc 180 gcatcgcgca cgcgctgccc ctcccctccg tcctccgctc caacttcacg ctcgccgacg 240 ggcccgccag ctccgccacg gggaaccccg aggagatcgc caagctgttc cccaacctgt 300 acggccagcc gtccgcggcc gtggtgccct cggccgagcc ggtgcccacc aagccgctca 360 agatcggcgt cgtgctctcc ggcggccagg cgccaggcgg gcacaatgtg atctgcggca 420 tcttcgatta cctgcaagag cgcgctaagg gcagcaccat gtacggattc aaaggaggcc 480 cagctgggat catgaagggc aagtacatcg agctcaatgc tgatttcgtc tacccctaca 540 ggaaccaggg tggatttgat atgatctgca gtggaaggga caagattgaa acaccagagc 600 agttcaagca agctgaagac acagtcaaca aacttgatct ggatggactt gttgttattg 660 gtggtgacga ctcaaacact aacgcatgcc tccttggtga atacttcagg ggaaggaatt 720 tgaagactcg tgttattggt tgccccaaga ccattgatgg agatctgaaa tgcaaggagg 780 tcccaataag ctttggattt gacactgctt gcaagatata ctccgaaatg attggcaatg 840 tgatgactga cgctcggtca acaggcaaat actatcactt tgtgaggctt atggggcgtg 900 ctgcttctca cattacatta gagtgtgctc tgcaaacaca ccctaacgtt tcactcattg 960 gcgaagaggt tgctgagaag aaggaaacac tcaagcaagt cacagactac attactgatg 1020 ttatctgcaa acgtgcagaa cttggttaca actatggagt tatccttatc ccggagggac 1080 ttattgattt catcccagag gttcaaaagc tcattgcaga gttgaatgaa attttggcac 1140 atgatgttgt tgacgaggca ggtgcttgga aaagcaagct tcaaccagaa tctaggcagc 1200 tgtttgactt cttgcccaac accattcagg agcagctttt gcttgaaaga gatccacatg 1260 gcaatgttca ggttgcgaaa attgaaactg agaagatgct tattgccatg gttgaaactg 1320 aattggagaa gagaagagct gcagggaagt actccgcaca tttcagaggc cagtctcact 1380 tctttggata tgaaggaaga tgtggtcttc ctaccaattt tgattctagc tactgctatg 1440 cattaggcta tggtgctggg gctcttctcc aatttggaaa gacaggactt atttcgtcgg 1500 ttggtaacct tgctgctcct gtggaagaat ggaccgtcgg aggaactcct ttgacggcat 1560 tgatggatgt tgagaggaga cacggcaagt tcaagccagt gatcaagaag gctatggtgg 1620 aacttgatgc cgcgccattc aagaagtttg cttccatgcg agatgaatgg gccatcaaga 1680 acagatacat cagccctggt cccatccagt tcagtggccc tggaagtgac gcgtcgaacc 1740 acaccttgat gttggagctt ggcgctcaga tatagagatg ctgcgttgta gagtgcacct 1800 ctttcatttc ttctctcctt acagttttga gagtggagac gaaaagctct cagagcgaca 1860 gtctccacat tgtggaatgt tcaataagag cttctggtat ggatgtcgca gcaataataa 1920 ctgattttag ctttttataa tctgaaaaaa aaaa 1954 20 3302 DNA Lolium perenne 20 gctcacttcc cccctccatc cctccttccc tttggcttcg cctccactct tcccatcccc 60 cgatctcgcc gtcgagcggc ggcggcgccg gcgacgatgg tgggcaacga caactggatc 120 aacagctacc tcgacgccat cctcgacgcc ggcaagtcgt ccatcggcgg cgaccgcccc 180 tcgctgctcc tccgcgagcg cggccacttc tccccggccc gctacttcgt cgaggaggtc 240 atcaccggct acgacgagac cgacctctac aagacatggc tccgcgcgaa cgcgatgcgg 300 agtccccagg agaggaacac gcggctggag aacatgacat ggaggatctg gaacctcgcc 360 aggaagaaga aggagttaga gaaagaagaa gcctgtcgtt tgttgaaacg gcatccagaa 420 actgagaaaa cgcgaactga tgctacggcc gatatgtctg aagatctctt tgatggcgaa 480 aagggagaag atgctggtga tccatctgtt gcatatggtg acagcaccac agggagctca 540 cctaagacca gttcagttga caagctatac atagtattga tcagcttaca tggtcttgtc 600 cgtggtgaga atatggagct aggccgagat tcagatactg gtggccaggt caaatatgtg 660 gttgagtttg ctaaagcatt gagttcatct cctggcgttt accgggtcga tttgctcaca 720 agacaaattg tagcaccaaa ttttgatcgt agttatggtg aacctgaaga aatgctggtt 780 tcgacaacct ttaaaaattc caagcatgaa aggggagtga acagtggtgg atacatcatt 840 cggataccat ttggtccaaa agacaagtac ttagctaaag aacatatgtg gcctttcatt 900 caagattttg ttgatggtgc actcagccat attttgcgga tgtcaaaaac cattggtgaa 960 gaaataggct gtgggcatcc agtatggcct gctgtgattc atgggcatta tgccagtgct 1020 ggagtagctg ctgccctgtt atcaggagca cttaacctgc ctatggcatt cacgggacat 1080 tttcttggga aagataaatt ggaagggctt ctcaaacaag ggcgacaatc aagggaacag 1140 ataaatatga catacaaaat aatgcgccga attgaggcgg aggaattatc tcttgacgca 1200 tctgaaattg ttattgctag tactaggcaa gagattgaag agcagtggaa cttgtatgat 1260 ggttttgagg tcatacttgc aaggaagctt cgagcaagag tcaagcgtgg tgctaactgc 1320 tatggccgtt atatgcctcg tatggttata attcctcctg gtgttgagtt tggccatgtc 1380 gttcatgatt ttgatatgga cggtgaagaa gaaaaccatg gcccagcatc tgaagatcca 1440 cctatctggt cgcagataat gcgcttcttt acgaatccta ggaagcctat gattctggct 1500 gttgcccgtc catatccgga aaagaatatc acatcacttg taaaagcatt tggtgaatgt 1560 cgcccactaa gagagcttgc gaatcttaca ctaatcatgg gtaaccgtga ggctatttca 1620 aagatgcaca acacaagtgc ttctgtcttg acatcagtgc tcacactaat tgatgaatac 1680 gatttgtatg gtcaagtggc ataccccaag caccataagc actctgaagt tcctgacatt 1740 tatcgtttgg ccacaagaac aaagggcgct tttgtaaatg tggcttattt tgaacaattt 1800 ggtgttacct tgatagaggc tgctatgaat ggtttgcctg ttattgctac aaaaaatgga 1860 gctcctgttg aaattaatca ggtgctcaac aatggtctcc ttgtcgatcc acatgatcag 1920 aatgccattg cagatgcact gtataaactt ctttctgaga agcaactctg gtcaagatgc 1980 agagaaaatg ggcttaaaaa tatccaccaa ttttcatggc ctgaacattg caagaatcac 2040 ttgtcaagga tattgactct tggtgcaaga tctcctgcta taggtagcaa agaggaaagg 2100 agcaatgcac ctatatcagg aaggaagcat ataattgtta tttctgtaga ctctgttaac 2160 aaggaagatc tagtacggat aatcagaaat gctattgagg ctgcacatac acagaacacg 2220 ccggcttcaa ctggtttcgt gctgtcaact tcactaacat tatcagagat ttgctcactg 2280 ctagtatctg taggcatgca tcctgctggc tttgatgcat tcatctgcaa tagtgggagt 2340 agcatttatt atccttcata ttctggtaat acgccaagca gttcaaaggt tacgcatgta 2400 atagatcaga atcaccaatc acatattgag tatcgttggg gaggagaagg tctaagaaag 2460 tatctagtga aatgggctac ttcagtggta gaaagaaagg gaagaattga aaggcaaatg 2520 atatttgaag attctgaaca ttcttctacc tattgtcttg catttaaagt ggtcaatcca 2580 aatcatctac ctcctctaaa ggagttgagg aagttgatga gaatccagtc actccgttgt 2640 aatgcgcttt acaaccacag tgctaccaga ctgtctgtaa ctcctattca tgcgtcccgc 2700 tctcaggcaa taaggtactt gtttatacgc tggggaatag agttgccgaa tattgtagtc 2760 cttgttggtg aaagtggtga ctcagattac gaagaactgc tagggggtct ccacaggacc 2820 ataatcctga agggtgactt caatattgcc gcaaacagaa tccacacggt caggagatac 2880 cccctacagg atgtcgtggc actggacagc tcaaatatca ttgaagtcga gggttgcact 2940 acagatgtca ttaagtctgc tctgcggcag attggggtac cgacacaata gcgttttgtg 3000 tttgcatgcg acacagagaa aagaaggggg aagaacaagc caaaccaagt actgtaccac 3060 aattcccata gttgatggga atgccgattt tgtttgtagg ttgtagagtg tgggtgtctt 3120 gagagagctg tgaataactt gcaacatcag tttgtactat tcacaaattt tgaagtgaaa 3180 cgatatgggt acgttatacg ttaaagacag gatatggatg cacttatcca taatgagaaa 3240 acatacttga agaagcctgg aaaggcagat aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3300 aa 3302 21 3171 DNA Festuca arundinacea misc_feature (1)...(3171) n = A,T,C or G 21 gttggtttcc cacccccaac ccattcgccg cctcccgccc gccgcccgat tgccgccacc 60 gccggcgcgc gcggctccgg cggcgaaacc tcctcctagc atcgggggag catggtgggc 120 gggatgtgcg ggaacgacaa ctggatcaac agctacctgg acgccatcct cgacgcgggg 180 aagggcgccc cgggcggagg cgccgggccc ggcggcggac gcggaggcgg cgggggtgga 240 gcgggcgacc gcccctcgct cctcctccgc gagcgcggcc acttctcccc cgcccggtat 300 ttcgtcgagg aggtcatcac cggctacgac gagaccgacc tctacaagac ctggtcacgc 360 gcgaacgcga tgcggagccc gcaggagagg aacacgcggc tggagaacat gacctggagg 420 atctggaacc tcgccaggaa gaagaaggag gtngaggctg aagaagccaa ccgtttgtta 480 aaacgtcgcc tagagacaga gaagccacgg actgatgccg ctgcagaaat gtctgaagat 540 ctctttgaag gacaaaaggg agaggatgct ggtgatgcat ctgttgccta cggtgacagc 600 tcggcttcaa acacacctag gatcagttcc atcgacaagc tatacatagt gttgatcagc 660 cttcatggcc tggtccgtgg tgagaacatg gaacttggcc gggattcaga cactagtggg 720 caggtcaaat atgttgtgga acttgctaaa gcattgagtt catgccctgg agtataccgg 780 gttgacctgt tgacaaggca aatattagca ccgaattatg atcgtggata tggtgaacca 840 tcagagacac tgttgccaac aaacttaaag aattttaaac atgaaagagg agagaacagt 900 ggtgcgtata tcaccagaat accatttgga ccaaaagaca agtatctagc taaagaacag 960 ctctggcctt atgttcaaga atttgttgat ggtgcactca gtcatatagt gcgcatgtcg 1020 aaaaccatag gtgaagaaat cggctgtggg catccaatgt ggcctgctgc gattcatggc 1080 cattatgcca gtgcaggagt agctgctgct ctactatctg gagcacttaa tgttcacatg 1140 atatttacag gccattttct tgggagagat aagttagaag ggcttctcaa gcaagggaaa 1200 cagacaaggg aagaaataaa tatgacatac aaaataatgc gccgaattga ggcagaggaa 1260 ctgtctcttg atgcgtctga aatagtgatt gcaagtacta ggcaagagat agaagagcaa 1320 tggaacttgt atgatggttt tgaagtcatg cttgcaagga aacttcgtgc aagagtcaag 1380 cgtggtgcaa actgctatgg tcgttacatg cctcgtatgg ttataattcc tccaggtgtt 1440 gaatttggcc atatgattca agattttgat atggatggtg aggaagatag cccatcacca 1500 gcatccgaag atccacctat ttggtctgag ataatgcggt tctttacaaa tcctaggaaa 1560 ccattgattc tggctgttgc tcgtccttac ccagaaaaga atattacaac gcttgtgagg 1620 gcttttggtg aatgccgacc attgagggag cttgctaacc taactctgat tatgggtaac 1680 cgtgaggcta tttccaaaat gagtaatatg agtgcagctg ttttgacatc agtgcttaca 1740 ctgattgatg aatatgatct gtatggtcaa gtggcatacc caaagcatca caaacactct 1800 gaagttcttg atatttatcg tttagcggcg agaacaaagg gtgcttttgt aaatgtagct 1860 tactttgaac aattcggtgt caccttgata gaggcggcca tgcatggttt acctgtaatt 1920 gcaacaaaaa atggagctcc tgttgaaatt caccaggtgc tgaacaatgg tcttcttgtt 1980 gatccccatg atcagaatgc aattgctgat gcactctata aacttctttc tgaaaaacaa 2040 ctttggtcaa gatgtcgaga gaatgggctg aaaaatatac accagttttc ttggcctgaa 2100 cattgcaaga attacttgtc aaggatatta actcttagcc caagataccc tgcttttgcg 2160 agcaatgatg accaaattaa ggctcctatc aagggaagaa agtatattat tgttattgcc 2220 gtagactctg ccagtaagaa agatctggcc tttatcatca gaaattctat tgaggctaca 2280 cggacagaaa cttcgtcagg ttcaacgggt ttcgtgttgt cgacttccct gacaatatca 2340 gagatacatt ctctattaat atccgcaggg atggttccca ctgattttga tgctttcata 2400 tgcaatagtg ggagtgattt attttaccct tcacagactg gtgattcacc aagcacttcc 2460 cgcgtaacat ttgcattaga ccgtaattac cagtctcgtg tcgagtatca ttggggcgga 2520 gaaggtttaa gaaagtatct agtgaagtgg gcttcttcag tagtggaaag gaggggcaga 2580 atggaaaagc aagttatttt tgatgattca gaacactcct cgacatgttg cctagcattt 2640 agagtggtca atccaaatta tttacctcct ttaaaggagc tgcagaagtt gatgagagtc 2700 caatcactac gttgtcatgc tctttataac cacagtgcta ctaggctatc tgtaattcca 2760 attcatgcat cacggtctca ggctataagg tacttatctg ttcgttgggg catagagttg 2820 ccaaatgtag tgattcttgt tggtgaaagc ggtgactcag actacgaaga gctgtttgga 2880 ggtcttcaca agacggttgt gctgaatggc gaattcaaca cccctgcaaa cagaatccac 2940 acagtcaggc ggtacccatt acaagatgtt atcgcgcttg attgctccaa catcgtagga 3000 gtccagggat gcagcactga ttgcatgagg tctactctag aaaagctcgg tataccgaca 3060 aaatgacact agtagacgtt tttttgtttt ttttgtatac gatgaaaaga aagaacgata 3120 cacatatagc aaatgaatac catcatttcc atgcttgatg gaaaaaaaaa a 3171 22 2092 DNA Festuca arundinacea 22 ccacccctct cctcactcca cgctccctcc ctccccccct ctcttccact cgcactttcc 60 gccctcgtct cctcctcttc ttcctcccgt cagccccgtt cctggcgcca ccttcttctt 120 cctcgcatgc gttgattcga tcaacgtact tttcccctct ctagatcctt ggccgaagaa 180 ttgataggcg aacgaggtga tcatcgttcg cacgacgtcc cggccatggt gcgcggcggc 240 ggcaacggcg aggtggagct ctccgtgggg gctggtggcg gcggcggcgg ggcgggaggc 300 ctggtggagc cgcccgtgcc gatcagtctc ggcaggctcg tcctcgccgg catggtcgcc 360 ggcggcgtgc agtatggatg ggccctccag ctctccctgc tcacccccta cgtccagact 420 ctgggacttt cacatgccct gacttcattc atgtggctct gcggcccaat tgctggctta 480 gtggttcaac catgtgttgg tctgtacagt gataagtgca cttccagatg gggaagacgg 540 aggccgttta ttatgacagg atgtgtgctc atatgcattg ctgttgtgat tgttggcttc 600 tcggctgaca ttggagctgc tctgggcgat agcaaggaag agtgcagtct ctaccatggt 660 cctcgctggc acgctgcaat tgtgtatgtg cttggattct ggcttcttga cttctccaac 720 aatactgtgc aaggtccagc tcgtgctctg atggctgatt tgtcaggcaa gtatggaccc 780 agtgctgcaa attcaatctt ctgttcttgg atggcgctag gaaatatcct agggtactcc 840 tctggttcca ccgataagtg gcacaagtgg tttccctttc ttcggacaag agcttgttgt 900 gaagcttgcg caaatctgaa aggcgcgttt ctggtggctg tgctgttcct gtgcatgtgt 960 ttggtgataa ctctgatctt cgccaaggag gtaccataca aacgaattgc acccctccca 1020 acaaaggcaa atggtcaggt tgaagttgaa cctagtggcc cgcttgcggt gttccaaggc 1080 atcaggaact tgccttccgg aatgccatcg gtgctccttg taactggcct cacctggctg 1140 tcctggttcc cgttcatcct ctacgacacg gactggatgg gtcgtgagat ttaccacggt 1200 gaccccaagg gcaccccagc tgagatgtcg gcgttccagg acggtgtcag ggctggcgcg 1260 ttcggactgc tactcaactc gatcatcctg gggttcagct cgttcctgat cgagccgatg 1320 tgcaagcggc taggcccgag ggtggtgtgg gtgtccagca acttcctcgt ctgcatcgct 1380 atggctgcca ccgccatcat cagctggtgg tctaccaagg aattccatga gtacgttcag 1440 catgccatta ccgccagcaa ggacatcaaa atcgtatgca tggccctctt cgcattcctc 1500 ggagtgcctc tcgccattct gtacagcgtt ccctttgcgg tgacggcgca gttggcggca 1560 agcaaaggag gcggccaagg gctgtgcacc ggcgtgctga atatctccat cgtcatccca 1620 caggtgatca tcgcgctggg ggcggggccg tgggaccagc tgttcgggaa gggcaacatc 1680 ccggccttcg ccgcggcctc cgccttcgcg ctcatcggcg gcatcgtcgg catattcctg 1740 ctgcccaaga tctccaggcg ctcgttccgg gccgtcagca ccggcggtca ctgaccgcgt 1800 cgggcgcctg cctgagcgcg ggcgaaagct cgatcgtgca ggccgggcgg ttccagctcg 1860 catgtgccaa tttttacata ggcttaaaaa taggtggctc tcgcttcaag actccgtaga 1920 gcagaataag aatgtgagga accgtatgtt tgtgtatgtg tgctagcgtg tgtaacagaa 1980 cgggcgaggg ggatgtggtc atccattacc ggctgggtgg tctgtaaagg ctatgtggcc 2040 gtcggatttg gatcggagcg cccttaatga gggcaggttg ttaaaaaaaa aa 2092 23 1600 DNA Festuca arundinacea 23 gcatctgcgt tgctgttgtg gtcgtcggct tctcggctga cattggagct gctctgggtg 60 atagcaagga agagtgcagt ctctaccatg gtcctcgctg gcacgctgca attgtgtatg 120 tgcttggatt ctggcttctt gacttctcca acaatactgt gcaaggtcca gctcgtgctc 180 tgatggctga tttgtcaggc aagtatggac ccagtgctgc aaattcaatc ttctgttctt 240 ggatggcgct aggaaatatc ctagggtact cctctggttc cacagataag tggcacaagt 300 ggtttccctt ccttcggaca agagcctgct gtgaagcttg cgcaaatttg aaaggcgctt 360 ttctggtggc tgtgctgttc ctgtgcttct gtttggtgat aactctgatc ttcgccaagg 420 aggtaccata caaacgaatt gcacccctcc caacaaaggc aaatggtcag gttgaagttg 480 aacctagtgg cccgcttgcg gtgttccaag gcttcaggaa cttgccttcc ggaatgccat 540 cggtgctcct tgtaactggc ctcacctggc tgtcctggtt cccgttcatc ctctacgaca 600 ccgactggat gggtcgtgag atttaccacg gtgaccccaa gggcacccca gctgaggcct 660 cggcgttcca ggacggtgtc agggctggcg cgttcggact gctactcaac tcgatcatcc 720 tggggttcag ctcgttcctg atcgagccga tgtgcaagcg gctgggcccg agggtggtgt 780 gggtgtccag caacctcctc gtctgcatcg ccatggccgc caccgccatc atcagctggt 840 ggtctaccaa ggaattccat gagtacgtcc agcatgccat caccgccagc aaggacatca 900 agatcgtatg catggtcctc ttcgcattcc tcggagtgcc tctcgccatt ctgtacagcg 960 ttccctttgc ggtgacggcg cagttggcgg caaacaaagg aggcggccaa gggctgtgca 1020 ccggcgtgct gaacatctcc atcgtcatcc cacaggtgat catcgcgctg ggggcggggc 1080 cgtgggacca gctgttcggg aagggcaaca tcccggcctt cgccgcggcc tccgccttcg 1140 cgctcatcgg cggcatcgtc ggcatattcc tgctgcccaa gatctccagg cactcgttcc 1200 gggccgtcag caccggcggt cactgaccgc gccgggcgcc gacctgagta cgggcgaaag 1260 ctcgcgtgca ggccgggcgg ttccagttcg catgtgccaa tttttacata ggcttaattt 1320 aggtggctct cgcttcaaga ctccgtagag cagaataaga atgtgaggaa ccgtatgctt 1380 gtgtatgtgt gctagtgtgt gtaacagaac gggcgaggga gtgtggtcat ccattaccgg 1440 ctgggtggtc tctgaaggct atgtggccgt cggatttgga tcggagcgcc cttaatgagg 1500 ccaggtgtca tccttgtgtt gtgacttgtg tagcaaacca aggttaaccg agtaaaggga 1560 aaagactgga tggtgcattt tcagcaacac aaaaaaaaaa 1600 24 2223 DNA Festuca arundinacea 24 aaaagaacac aaacccacac caccaccacc acctctcctc actccacgct cccctcctcc 60 ctcgcatcac acacaccctc gtctcctcct cttcttcctc ccgtcagccc cgttcctggc 120 gctaccatct tcttcctcgc atgcgttgat tcgatcaacg tacttttccc ctctctagat 180 ccttgttggc cgaagaattg ataggcgaac gaggtgatca tcgttcgcac gacgtcccgg 240 ccatggtgcg cggcggcggc aacagcgagg tggagctctc cgtgggggcc ggtggcggcg 300 gcggcggggc gggaggcctg gtggagccgc ccgtgccgat cagcctcggc aggctcgtct 360 tcgccggcat ggtcgccggc ggcgtgcagt atggatgggc cctccagctc tccctgctca 420 ccccctacgt ccagactctg ggactttcac atgccctgac ttcattcatg tggctctgcg 480 gcccaatagc tggcttagtg gttcaaccat gtgttggtct gtacagtgat aagtgcactt 540 ccagatgggg aagacggagg ccgtttatta tgacaggatg tgtgctcata tgcattgctg 600 ttgtgattgt tggcttctcg gctgacattg gagctgctct gggcgatagc aaggaagagt 660 gcagtctcta ccatggtcct cgctggcacg ctgcaattgt gtatgtgctt ggattctggc 720 ttcttgactt ctccaacaat actgtgcaag gtccagctcg tgctctgatg gctgatttgt 780 caggcaagta tggacccagt gctgcaaatt caatcttctg ttcttggatg gcgctaggaa 840 atatcctagg gtactcctct ggttccacag ataagtggca caagtggttt cccttccttc 900 ggacaagagc ctgctgtgaa gcttgcgcaa atttgaaagg cgcttttctg gtggctgtgc 960 tgttcctgtg cttctgtttg gtgataactc tgatcttcgc caaggaggta ccatacaaac 020 gaattgcacc cctcccaaca aaggcaaatg gtcaggttga agttgaacct agtggcccgc 080 ttgcggtgtt ccaaggcttc aggaacttgc cttccggaat gccatcggtg ctccttgtaa 140 ctggcctcac ctggctgtcc tggttcccgt tcatcctcta cgacaccgac tggatgggtc 200 gtgagattta ccacggtgac cccaagggca ccccagctga ggcctcggcg ttccaggacg 260 gtgtcagggc tggcgcgttc ggactgctac tcaactcgat catcctgggg ttcagctcgt 320 tcctgatcga gccgatgtgc aagcggctgg gcccgagggt ggtgtgggtg tccagcaacc 380 tcctcgtctg catcgccatg gccgccaccg ccatcatcag ctggtggtct accaaggaat 440 tccatgagta cgtccagcat gccatcaccg ccagcaagga catcaagatc gtatgcatgg 500 tcctcttcgc attcctcgga gtgcctctcg ccattctgta cagcgttccc tttgcggtga 560 cggcgcagtt ggcggcaaac aaaggaggcg gccaagggct gtgcaccggc gtgctgaaca 620 tctccatcgt catcccacag gtgatcatcg cgctgggggc ggggccgtgg gaccagctgt 680 tcgggaaggg caacatcccg gccttcgccg cggcctccgc cttcgcgctc atcggcggca 740 tcgtcggcat attcctgctg cccaagatct ccaggcactc gttccgggcc gtcagcaccg 800 gcggtcactg accgcgccgg gcgccgacct gagtacgggc gaaagctcgc gtgcaggccg 860 ggcggttcca gttcgcatgt gccaattttt acataggctt aatttaggtg gctctcgctt 920 caagactccg tagagcagaa taagaatgtg aggaaccgta tgcttgtgta tgtgtgctag 980 tgtgtgtaac agaacgggcg agggagtgtg gtcatccatt accggctggg tggtctctga 040 aggctatgtg gccgtcggat ttggatcgga gcgcccttaa tgaggccagg tgtcatcctt 100 gtgttgtgac ttgtgtagca aaccaaggtt aaccgagtaa agggaaaaga ctggatggtg 160 cattttcagc aacacaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 220 aa 2223 25 2042 DNA Lolium perenne 25 gatttccaac tccttccgtg gcggcaccgc cgtggcccga cgggattgga gccaccggaa 60 caagattctt tgctcgctgc tctccccctt cttcttcctc ctcactgctc ccgccggcac 120 agtcgggagc tcacaatcca gcagccacac caccggcggc gtaggcgtag gcggagcggg 180 ggtcgaaaag ttcaatccga cacaagatgc cgcccccgcg gcggccgacc accggcggca 240 ccaccaccac ctccgccgcc ctccccccac cccgcaaggt cccgctgcgc tccctcctgc 300 gcgccgcctc ggtcgcctgc ggggtgcagt tcgggtgggc gctgcagctc tcgctgctca 360 cgccctacgt gcaggagctg ggcatcccgc acgccttcgc gtcgctcgtc tggctctgcg 420 gcccgctctc cggcctgctg gtgcagccgc tcatcggcca cctctccgac cgcatcgcgc 480 ccgccgactc cccgctcggc cgccgccggc ccttcatcgc cgcgggcgcc gcctccatcg 540 ccttctccgt gctcaccgtc ggcttctcgg ccgatctggg gcggctgttc ggggacaacg 600 tccggcccgg gtcgaccagg tacggcgcga ttattgtgta tatgatcggc ttctggctgc 660 tggatgtcgg caacaacgcc acccaggggc cctgcagggc cttcctcgct gacctcaccg 720 agaatgaccc gaggaggact cggatcgcca acgcctattt ctcgctcttc atggccctgg 780 ggaacatcct cgggtatgcc accggagcgt acagcggctg gtacaagata tttcccttca 840 ccatcaccga gtcatgtggc gtcagctgtg ccaacctcaa gtctgcgttc ctgctagaca 900 tcatcatcct ggcaattacg acatacgtca ccgtggtaac ggtgcaagac aacccgacat 960 tcgggagcga tgaagcagcg ccacgtccga gcagccacga agaggaggct ttcctcttcg 1020 agctctttgg gtcattcaag tacttcacga tgcctgtctg gatggtcctg atcgtcactt 1080 ccctcacctg gatcgggtgg ttcccgttca tcctcttcga taccgactgg atgggccggg 1140 aaatctaccg agggagcccg gagatcgtcg ccgacaccca gaagtaccat gacggtgtga 1200 gaatgggttc ctttggcctc atgctcaatt cagtccttct cgggatcacg tcggtcgtga 1260 cggagaagct gtgcaggaag tggggggctg ggctcgtatg gggtgtttcc aatattatca 1320 tggctctctg ctttgtggcg atgctcgtta taacgtacgt ggcgcagaat ttggactatg 1380 gacctagcgg agcacctccg accggcatcg ttgttgcttc cctcacagtt ttcacgattc 1440 tgggagcacc cttgtcgatc acgtacagta taccatacgc gatggctacg agccgtgttg 1500 agaatcttgg gcttggccag ggtctagcaa tgggtattct caatttatct atcgtcatac 1560 cacagatcat cgtgtcactg ggcagtgggc cgtgggactc gctatttggc ggagggaacg 1620 cgccatcgtt ttgggtggcg gctgccgcgt ccttcattgg cgggctggtg gccatcctgg 1680 gtctcccccg agcccgtatt gcgccaaaga agaggagcca gcgatgatga tcagagtaca 1740 ttatttcagg ttgtctgtat atcgtggctc taagttaagt tgcaagttgc aaccattctt 1800 tgctctatca tccagtggat taggcatggg tatctgtctg tatatcagtt cgatggggaa 1860 agaaatacag ctctggaatc tggtcttctt ttctttcctc catgagctgt tgtttttttc 1920 gcgtctccgt gtcaaacatg gtcggttgta tcttgttgta tgttggattt ttatggtttt 1980 gtcggagatg gtgggtgaat gaatgaataa aaagtcggca gggttttgct tgaaaaaaaa 2040 aa 2042 26 1930 DNA Festuca arundinacea 26 gctcctagta cagtcgggag ttcagaattc tgcagacgca ccaccgcgaa ccaaggaggc 60 ggcggcggag gcgtagggga agtgggccat ccgagagaag atgccgcccc cgcggcggcc 120 caacgccggc ggcaccacct ccgcccctct acccccaccc cgcaaggtcc cgctccgctc 180 cctccttcgg gcggcctccg tcgcctgcgg ggtccagttc gggtgggcgc tgcagctctc 240 cctcctcacg ccctacgtgc aagaactcgg catcccgcac gccttcgcct ccctcgtctg 300 gctctgcggc ccgctctccg gcctcctggt ccagcccctc atcggccacc tgtccgatcg 360 catcgcgccc gccgactccc cgctcggccg ccgccggccc ttcatcgccg cgggggcggc 420 gtccatcgcc ttctctgttc tcacggtcgg cttctcggcg gatttgggga ggctgttcgg 480 ggacaacatc cggccgggat ccaccaggtt cggcgcgatt attgtttaca tgatcgggtt 540 ctggctgctg gatgtaggga acaacgccac ccaggggccc tgcagggcct tcctcgcaga 600 cctcaccgag aatgacccga ggaggactcg gatcgccaac gcctatttct cgctcttcat 660 ggccctgggg aacatcctcg ggtacgccac tggagcgtac agcggctggt acaagatatt 720 ccccttcacc atcaccgagt cctgtggcgt cagctgtgcc aacctcaagt ctgcgttcct 780 gctagacatc atcatcctgg caattacgac atacgtcacc gtggtaacgg tgcaagacaa 840 cccgacattc gggagcgatg aagcggcacc gcgtccgagc agccacgaag aggaggcttt 900 cctcttcgag ctcttcgggt cattcaagta cttcacgctg cctgtctgga tggtcctgat 960 cgtcacttcc ctcacctgga tcgggtggtt cccgttcatc ctcttcgata ctgactggat 1020 gggccgggaa atctaccgag ggagcccgga gatcgtcgcc gacacccaga agtaccatga 1080 tggtgtgaga atgggttcct ttggcctcat gctcaactca gtccttctcg ggatcacatc 1140 ggtcgtgatg gagaagctgt gcaggaagtg gggggctggg ctcgtatggg gtgtttccaa 1200 tattatcatg gctctctgct ttgtggcgat gctcattata acgtacgtgg cgaagaattt 1260 ggactatgga cctagcggag cacctccgac cggcatcgtc gttgcttccc tcgcagtttt 1320 cacgattctg ggagcaccct tgtcgatcac gtacagtata ccgtacgcga tggctacgag 1380 ccgtgttgag aatcttgggc ttggccaggg tctagcaatg ggcattctca atttatctat 1440 cgtcatacca cagatcatcg tgtcactggg cagtgggcca tgggactcgc tttttggcgg 1500 agggaacgcg ccatcgtttt gggtggcggc cgccgcgtcc ttcattggcg ggctggtggc 1560 catcctgggt ctcccccgag cccgcattgc gccaaagaag agaagccagc gatgatgatc 1620 agagtacatt gtttcaggtt gtctgtatat cctggctctg agttaagttg caagttgcaa 1680 ccattctttg gtctatcatc cagttgatta ggcatgggta tctgtctgta tatcggttcg 1740 atggggaaag aaatacagct ctggaatctg gtcttgtttt ccttcctcca tgagctgttg 1800 tttttttcgc gtctccgtgt caaacatggt cggttgtatc ttgttgtatg ttggattttt 1860 atggttttgt ccgagatggt gggtgaatga atgaataaaa agtcggcagg gttttgcttc 1920 aaaaaaaaaa 1930 27 1911 DNA Lolium perenne 27 ggcccagctc ccgcaactac caacctccca tataaatccc tggtacacag agttcttcag 60 cagcagccta gtgatctccc cattaccccg cacgatcaat cctcgatatg gtggaccaag 120 atcacgatgg gcggcggcga caggaggagg cgacggcggt ggcggcgtcg tcagttccgt 180 tgctggagaa gaagcccggc gacgtgccgt actacgtgga ggggtgcccg gggtgcgcgg 240 tggaccggag gaaggcgacg gacccaggca tcccctacgg cagcttcatc tacatctggg 300 tcgtcatcct ctgcaccgct ataccaatat cgtcgctatt ccccttcttg tatttcatga 360 taagagactt gcacatcgcg gaaagaacag aagatattgg gttctatgct gggtttgtag 420 gtgccgcttt tatgtttggt agatgtttga cttcaactat ttggggcata gcagcagatc 480 gtattgggag gaagccagtt gtgatatttg gtgttttctc tgtggtaata tttaatgctt 540 tgtttgggct tagtgttacc tactggatgg caatagctac aaggtttctc cttggcgctt 600 taaatggttt acttggacca atgaaggcat atgctattga agtttgccgg cctgaacacg 660 aagctctagc actatcactt gtcagcacag catggggaat aggtctcatc attggtcctg 720 ctcttggagg ctaccttgca ctgcctgcag aaaaatatcc aaatatcttt tcgcctgact 780 cgctatttgg aaggttcccg tacttcttac catgcttatg cacatcagtg tttgctgccg 840 ctgttctgat aggctgcata tggatgccgg agacgttgca caagcataaa gtaaatgaga 900 ataggaatca aagtgtcgaa tctttggagg cccatctgat tgatccaaaa gagaaggttg 960 aacaaagtaa tagtccggat accaagaaga gcttattcaa gaattggcca ttaatgtcat 1020 ccataattgt ttattgtgtc ttctccttcc acgacatggc ttacacagag gtgttctctc 1080 tgtgggctga aagtgacagg acatatggtg gactcagttt gtcatctgaa gatgtaggcc 1140 aaacacttgc aattacaggc tccagtcttc ttgtgtatca actcttcttg tacccgcgca 1200 tcaacagggt tcttggacct atcaaatcat ctcaaatcgc agctggcata tgcataccta 1260 ttctctttgc ctacccctat atgacgtacc tgtcagaacc tggattatca attgttctga 1320 atattgcatc agtcataaaa aataatcttg gtgttaccat cattacaggc actttcatcc 1380 ttcaaaataa tgccgtgcct caggaccaaa gaggtgcagc aaacgggtta gccatgactg 1440 gaatgtcctt tttcaaggca gttgctcctg caggcgctgg cattgtgttt tcatgggcgc 1500 agaaacgaca acatgctttc ttctttccag gtgatcagat ggtgttcttt ctgctgaaca 1560 tcattgagct ccttggactt ctcctcacat tcaaattctt cctggccgtg ccagataaat 1620 ctgatagcaa ctagctgttc agctattgtt ctagatattt tttggataat tattcagatg 1680 gcgatgactc atggatcttg catccgcgag gagaagggct taaggcgccc ttataacagg 1740 attaagaggt ggccaggtac aagttgtgtt gttgtgtgta agatgtagga gcactccagt 1800 agaggagatg caacatttgt aagtagtgat gtagtaagag actgcggctc ggaataattg 1860 gcggaattgg actcagcata attgctatat ctctcgctat caaaaaaaaa a 1911 28 2039 DNA Lolium perenne 28 gctccctctc gccacatcac ccccgcccag cccactccct ccatcaattc ctccccacat 60 ttgattcatc cctgctccca tcccatccca ttccgttgca ttccaccggt ccttccccga 120 tccgccgatt cgttccagtt gcagggccga tgtcatcgat gcagttcagc agcgtgctcc 180 cgctggaggg caaagcgtgc gtgtgcccgg tgaggagcgc caacaacggg tgcgagaggc 240 tcaaggtcgg ggacagcagc agcctcaggc acgagatggc gctgaggagg aagtgcaacg 300 gcgccagagg aggaggcgcc gccaacggcg cgcagtgcgt gctcacctcc gacgccagcc 360 cggacaccct tgttgtccga tcgtccttcc ggaggaacta cgccgatccg aacgaggttg 420 cggcggtcat actgggcggc ggcaccggga ctcaactctt ccctctcacc agcacaaggg 480 ctacgcctgc tgttcctatt ggaggatgtt acaggcttat cgatattccc atgagcaact 540 gcttcaacag tggcataaac aagatattcg tgatgactca gttcaactcg gcatctctta 600 atcgtcacat tcaccgtacg tacctcggcg gggggatcaa tttcactgat ggatctgttg 660 aggtattggc cgcaacgcaa atgcctgggg aggctgctgg atggttccgg ggaaccgcag 720 atgcggtcag aaaatttatc tgggtacttg aggactatta taagcataaa tctatcgagc 780 acattttgat cttgtcgggg gaccagcttt atcgcatgga ttacatggag cttgtgcaga 840 aacatgttga tgacaatgct gacattactc tatcatgtgc ccctgttgga gaaagccggg 900 catccgaata tggactagtg aagttcgaca gttcaggtcg cgtgatccag ttttctgaga 960 agccaaaggg cgcggacttg gaagcaatga aagtggatac cagctttctc aattttgcca 1020 tagatgatcc agccaaaaat ccctacattg cttccatggg agtttatgtc ttcaaaagag 1080 aagttctttt gaaccttcta aagtcaagat acacagaact acatgacttt gggtctgaaa 1140 tcctcccgag agctctacat gaccacaatg tacaggcata tgtcttcact gactactggg 1200 aggacattgg aacaatcaga tcgttcttcg atgcaaacat ggcactctgc gagcagcctc 1260 caaagtttga gttttatgac ccaaagactc ccttcttcac ttcgcctcga tacttgccac 1320 caacaaagtc cgataagtgc aggatcaaag aggcgatcat ttcacacggc tgcttcctgc 1380 gcgaatgcac cattgagcac tctatcatcg gcgtccgttc acgcctaaac tccggatctg 1440 tgcttaagaa cgcgatgatg atgggtgcgg atctgtacga gaccgaggac gagatctcgg 1500 ggctgctgtc cgagggcaag gtccccatcg gtgttgggga gaactccaag ctcagcaact 1560 gcatcatcga catgaacgct aggatcggaa gggacgtggt catcgcgaac agcgagggcg 1620 tccaggaggc tgatcggcca gaggaagggt actacatcag gtccgggatc gtggtgatac 1680 tgaagaacgc aaccgtgaag gacggcaccg tggtgtagaa cgccgcctgc gccgcggcaa 1740 ccgcatcttt ttcgagagtt gttggtagcc cagagctgcc gacctgaagt tcattcagac 1800 gaggaagaag ataggatccc tggcgggacg gtagaagttg ggagctggga caggagacgg 1860 ctcatgcagc aagcatcagt agcaaagcaa gtactcctag tagtagtcgt tcttcccctg 1920 taataataaa gctgcgtgcg tgcgcgtcga gttgaagtgg cagcagactc ttctggggga 1980 tcgatcctgt aaataaaact tgaaaaatat gggatttttc cgttgcctca aaaaaaaaa 2039 29 2063 DNA Festuca arundinacea 29 gccgtggtgg cgtttcgtcg ccggccggat aaaaatactt gtgcttcttc tatctccgtc 60 tgtgcaagag agagagagag agcagcggcg tttgtcgccg gtgggctggt tcggcgcgtg 120 tcgctgaggc cagccccaac agagttcatc actattgcag cagggccgat gtcatcgatg 180 cagttcagca gcgtgctccc gctggagggc aaagcgtgcg tgtgcccggt gaggagcgcc 240 aacaacgggt gcgagaggct caaggtcggg gacagcagca gcctcaggca cgagatggcg 300 ctgaggagga agtgcaacgg cgccagaggg ggaggcgccg ccgacggcgc gcagtgcgtg 360 ctcacctccg acgccagccc ggacaccctt gtcgtccggt cgtccttccg gatgaactac 420 gccgatccga acgaggttgc ggcggtcata ctgggcggcg gcaccgggac tcagctcttc 480 cctctcacca gcacaagggc tacgcctgct gttcctattg gaggatgtta caggcttatc 540 gatattccca tgagcaactg cttcaacagt ggcataaaca agatattcgt gatgactcag 600 ttcaactcgg catctcttaa tcgtcacatt caccgcacgt acctcggcgg ggggatcaat 660 ttcactgatg gatctgttga ggtattggcc gcaacgcaaa tgcctgggga ggctgctgga 720 tggttccggg gaaccgcaga tgcagtcaga aaatttatct gggtacttga ggactattat 780 aagcataaat ctatcgagca cattttgatc ttgtcggggg accagcttta tcgcatggat 840 tacatggagc ttgtgcagaa acatgttgat gacaatgctg acattactct atcatgtgcc 900 cctgttggag aaagccgggc atccgaatat ggactagtga agttcgatag ttcaggtcgc 960 gtgatccagt tttctgagaa gccaaagggc gcggacttgg aagcaatgaa agtggatacc 1020 agctttctca attttgccat agatgatcca gccaaaaatc cctacattgc ttccatggga 1080 gtttatgtct tcaaaagaga agttcttttg aaccttctaa agtcaagata cacagaacta 1140 catgactttg ggtctgaaat cctcccgaga gctctacatg accacaatgt acaggcatat 1200 gtcttcactg actactggga ggacattgga acaatcagat cgttcttcga tgcaaacatg 1260 gcactctgcg agcagcctcc aaagtttgag ttttatgacc caaagactcc cttcttcact 1320 tcgcctcgat acttgccacc aacaaagtcc gacaagtgca ggatcaaaga agcgatcatt 1380 tcacacggct gcttcctgcg cgaatgcacc attgagcact ctatcatcgg cgtccgttca 1440 cgcctaaact ccggatctgt gcttaagaac gcgatgatga tgggcgcgga tctgtacgag 1500 accgaggacg agatctcggg gctgctgtcc gagggcaagg tccccatcgg tgtcggggag 1560 aactccaagc tgagcaactg catcatcgac atgaacgcta ggatcggaag ggacgtggtc 1620 atcgcgaaca gtgagggcgt ccaggaggct gatcggccag aggaagggta ctacatcagg 1680 tccgggatcg tggtgatact gaagaacgca accgtgaagg acggcaccgt ggtgtagaac 1740 gccgcctgcg ccgcggcagc tgcatctttt tcgagagttg gcggtagccc agagctgccg 1800 acctgaagtt cattcagacg aggaagaaga tagggtccct ggcgggaccg tagaagttgg 1860 gagctgggag cctgggacga gagacggctc atgcagcaag catcagtagc aaagcaagta 1920 ctcctagtaa tagtcgttct tcccctgtaa taataagctg cgtgcgtgcg tcgagttgaa 1980 gtggcagcag actcttctgg gggatcgatc ctgtaaataa aacttgaaaa atatgggatt 2040 tttccgttgc ctcaaaaaaa aaa 2063 30 1815 DNA Lolium perenne 30 gcggacgcgc catgaccgga gctccgccat ccaccgtaat ggcgatgggt gcggccacct 60 ccccttgcaa gatcttgagc gccacgcaac gtgcctccac cgcggcggct tcggcatcca 120 cctcccgcga gtccgtctcc ctccgcgcac cacggggacg gcgccagcgc ccgcgcccgc 180 gcgggttggc cttgtccctg gctccagcgc gacggccgtt tgtcttctcc ccgcgcgccg 240 tgtcagactc caagagctcc cagacctgcc tcgaccctga cgcaagcacg agtgttctcg 300 gaatcattct gggaggtggt gcagggacta gattgtatcc tctgacaaag aagcgtgcga 360 agcctgctgt gccattgggt gccaactaca ggcttattga tattcctgtc agcaattgtt 420 tgaacagcaa tatatcaaag atctatgtgc tgacacagtt caactctgct tctcttaatc 480 gtcatctctc acgagcttat gggagcaaca ttggaggata caagaatgaa ggatttgttg 540 aagtcctcgc ggcacagcag agcccagaca atcctaactg gtttcagggt actgcagatg 600 ctgtaaggca gtatttatgg ctattcgagg aacataatgt tatggaatat ctaattcttg 660 ccggagatca cttgtaccga atggactatg aaaagtttat tcaggcgcac agagaaacag 720 atgctgatat tactgttgcc gccttgccca tggatgagga acgtgcaact gcatttggcc 780 ttatgaaaat cgatgaagaa gggaggatag ttgaatttgc agagaaacca aaaggagagc 840 agttgaaagc aatgatggtt gatacaacca tacttggtct tgatgacgtg agggcaaagg 900 aaatgcctta tatcgctagc atgggtatct acgttattag caaacatgta atgctccagc 960 ttctccgtga ccaatttcct ggagctaatg actttggaag tgaggttatt cctggtgcga 1020 ctagcactgg aatgagggta caagcatact tatatgatgg ttactgggaa gatattggta 1080 caattgaggc attctataac gcaaatttgg gaattaccaa aaagccaata ccagatttca 1140 gtttctatga tcgttctgct ccaatttaca cacaacctcg acacttgcct ccttcaaagg 1200 ttcttgatgc tgacgtgaca gacagtgtta ttggcgaagg atgtgttatt aaaaactgca 1260 agatacacca ttcagtagtt ggactgcggt cctgcatatc tgaaggtgca attatagagg 1320 acacattact aatgggcgca gactactatg agactgaagc tgacaagaaa ctccttgccg 1380 acaaaggtgg gattcccatt ggtattggaa agaattcaca catcagaaga gcaatcattg 1440 acaagaatgc tcgtattgga gacaacgtga agataatcaa tgttgacaat gttcaagaag 1500 cagcccggga gactgatgga tacttcatca aaagtggcat cgtaactgtg atcaaggatg 1560 ctttactccc aagtgggaca gtcatatgaa acagatgcaa aatatgtggc aagtcacggc 1620 gcttcttgta tcattctgca atcaaccaat gaggtcgcca gaagatcata agagcaataa 1680 aaaggagtgc cctgcaaggc acttcatctt ttttctccct taatgtatta gcaaccgtaa 1740 tgtacaagca acttgcatcc agatgttctg gagatcgaat atacctgctt gcatcttgtt 1800 gtttcaaaaa aaaaa 1815 31 1873 DNA Festuca arundinacea 31 gcccatgacc cgagctccgc catccaccgt aatggcgatg ggtgcggcca cctccccttg 60 caagatcttg agcgccacgc aacgtgcctc cgccgcggcg ccttcggcat ccacctcccg 120 cgagtccgtc tgcctcctcc gcgcgccacg gggacggcgc cagcgcccgc gcgggttggc 180 cttgtccctg gctccagcgc gacggccgtt tgtcttctcc ccgcgcgccg tgtcagactc 240 caagagctcc cagacctgcc tcgaccctga cgcaagcacg agtgttctcg gaatcattct 300 gggaggtggt gcagggacta gattgtatcc tctgacaaag aagcgtgcga agcctgctgt 360 gccattgggt gccaactaca ggcttattga tattcctgtc agcaattgtt tgaacagcaa 420 tatatcaaag atctatgtgc tgacacagtt caactctgct tctcttaatc gtcatctctc 480 acgagcctat gggagcaaca ttggaggata caagaatgaa ggatttgttg aagtcctcgc 540 ggcacagcag agcccagaca atcctaactg gtttcagggt actgcagatg ctgtaaggca 600 gtatttatgg ctattcgagg aacataatgt tatggaatat ctaattcttg ccggagatca 660 cttgtaccga atggactatg aaaagtttat tcaggcgcac agagaaacag atgctgatat 720 tactgttgcc gccttgccca tggatgagga acgtgcaact gcatttggcc ttatgaaaat 780 cgacgaagaa gggaggatag ttgaatttgc agagaaacca aaaggagagc agttgaaagc 840 aatgatggtt gatacgacca tacttggcct tgatgacgtg agggcaaagg aaatgcctta 900 tatcgctagc atgggtatct acgttattag caaacatgta atgctccagc ttctccgtga 960 ccaatttcct ggagctaatg actttggaag tgaggttatt cctggtgcga ctagcactgg 1020 aatgagggta caagcatact tatatgatgg ttactgggaa gatattggta caattgaggc 1080 attctataac gcaaatttgg gaattaccaa aaagccaata ccagatttca gtttctatga 1140 ccgttctgct ccaatttaca cccaacctcg acacttgcct ccttcaaagg ttcttgatgc 1200 tgacgtgaca gacagtgtta ttggcgaagg atgtgttatt aaaaactgca agatacacca 1260 ttcagtagtt ggactgcggt cctgcatatc tgaaggcgca attatagagg acacattact 1320 aatgggtgca gactactatg agactgaagc tgacaagaaa ctccttgccg acaaaggtgg 1380 gattcccatt ggtattggaa agaattcaca catcagaaga gcaatcattg acaagaatgc 1440 tcgtattgga gacaacgtga agataatcaa tgttgacaat gttcaagaag cagcccggga 1500 gacggatgga tacttcatca aaagtggcat cgtaactgtg atcaaggatg ctttactccc 1560 gagtgggaca gtcatatgaa acagatgcaa aatatgtggc aagtcacggc acttcttgta 1620 tcattctgca atcaaccaat gaggtcgcca gaagatcata agagcaataa aaaggagtgc 1680 cctggaaggc acttctccat cttttttctc ccttaatgta ttaggaaccg taatgtacaa 1740 gcaacttgca tccagatgtt ctggagatcg aaaatacctg cttgcatctt gttgtttcaa 1800 atatgaagtg tactagataa agccccgcat gttttttcac gatattacaa aactttgtag 1860 ttgaaaaaaa aaa 1873 32 1494 DNA Festuca arundinacea 32 gcaacaagga gcaactgtca ctcattcatc tgtcgtctcc tgcttccctc aagcttagat 60 cgattgcagc cggccgggga ctgttgagct accactgccg gtcgctggta cgagcggacg 120 taaggagaga tccagatggc cgcgacgatg accgtggagg aggtgaggaa ggcgcagcgg 180 gcggaggggc cggcgacggt gctggccatc ggcacggcga cgcccgctaa ctgtgtctac 240 caggctgact acccggacta ctacttcaag atcaccaaga gcgaccacct cgccgatctc 300 aaggagaagt tcaagaggat gtgcgacaag tctcagatca ggaagaggta catgcacctg 360 acggaggaga tcctggagga gaaccccaac atgtgcgcgt acatggcgcc gtcgctggac 420 gcgcgccagg acatagtcgt cgtcgaggtc ccgaagctcg ggaaggcggc ggcacagaag 480 gcgatcaagg agtggggcca gccgcggtcc aagatcaccc acctcgtctt ctgcactacc 540 tctggtgtgg acatgccagg cgccgactac cagctcacca agatgctcgg cctgcgcccg 600 tcggtgaagc gcctcatgat gtaccagcag ggctgcttcg ccggcggcac ggtgctccgc 660 ctcgccaagg acctggctga aaacaaccgc ggcgcgcgcg tgctggtggt ctgctcggag 720 atcacggccg tgaccttccg cggcccgcac gagtcacacc tcgactcgct ggtcggccag 780 gcgctcttcg gggacggcgc tgccgcggtg ataatcggcg ccgaccccga cgtgtccgtc 840 gagcgcccgc tgttccagct ggtgtcggtg agccagacca tcctgccgga ctcggagggc 900 gccatcgacg gccacctcag ggaggtcggc ctcaccttcc acctcctcaa ggacgtgccc 960 gggctcatct ccaagaacat cgagcgcgcc ctggaggaag ccttcaagcc gctcggcatc 1020 gacgactgga actccgtctt ttgggtggcg cacccgggcg ggccggcgat cctcgacatg 1080 gtggaggcca aggtaaacct caacaaggag cggatgcgtg ccaccaggca cgtcctctcc 1140 gagtacggca acatgtccag cgcatgcgtc ctcttcatca tggacgagat gcgcaagcgc 1200 tccgccgagg atggccacac caccaccggc gagggaatgg attggggcgt cctctttggc 1260 ttcgggcccg gcctcaccgt cgagaccgtt gtcctccaca gcatgcccat tgccgctgat 1320 gccaccgctt gaccgatggt tccatctccg tttatctgcc acattgatga atacctacta 1380 ctactaccgc cgccgccgct gctatccaaa gtaatttgta ttgtattcat gcatacctgg 1440 tttgtatttg ttggtaggat tcgttctgct attatgtcgc ttgtgttgcg taca 1494 33 1661 DNA Festuca arundinacea 33 gcaacaagca gcaactgtca ttcattcatc tgtcgtctcc tgcttccctc aaacttagat 60 cgatcgcagc cggccgggga ctggtgagct accactgtcg gtcgctggta cgagcggacg 120 caaggagaga tccagatggc cacgacgatg accgtggagg aggtgaggaa ggcgcagcgg 180 gcggaggggc cggcgacggt gctagccatc ggcacggcga cgcccgctaa ctgtgtctac 240 caggctgact acccggacta ctacttcaag atcaccaaga gcgaccacct cgccgacctc 300 aaggagaagt tcaagaggat gtgcgacaag tctcagatca ggaagaggta catgcacctg 360 acggaggaga tcctggagga gaaccccaac atgtgcgcgt acatggcgcc gtcgctggac 420 gcgcgccagg acatagtcgt cgtcgaggtc ccgaagctcg ggaaggcggc ggcgcagaag 480 gcgatcaagg agtggggcca gccgcggtcc aagatcaccc acctcgtctt ctgcaccacc 540 tccggtgtgg acatgccggg cgccgactac cagctcacca agatgctcgg cctgcgcccg 600 tcggtgaagc gcctcatgat gtaccagcag ggctgcttcg ccggcggcac ggtgctccgc 660 ctcgccaagg acctggctga aaacaaccgc ggcgcgcgcg tgctggtggt ctgctcggag 720 atcacggccg tgaccttccg cggcccgcac gagtcacacc tcgactcgct ggtcggccag 780 gcgctcttcg gggacggcgc tgccgcggtg atcatcggcg ccgaccccga cgtgtccgtc 840 gagcacccgc tgttccagct ggtgtcggcg agccagacca tcctgccgga ctcggagggc 900 gccatcgacg gccacctcag ggaggtcggc ctcaccttcc acctcctcaa ggacgtgccc 960 gggctcatct ccaagaacat cgagcgcgcc ctggaggaag ccttcaagcc gctcggcatc 1020 gacgactgga actccgtctt ctgggtggcc cacccgggcg ggccggcgat cctcgacatg 1080 gtggaggcca aggtaaacct caacaaggag cggatgcgcg ccaccaggca cgtcctgtcc 1140 gagtacggca acatgtccag cgcatgcgtc ctcttcatta tggacgagat gcgcaagcgc 1200 tccgccgagg atggccacac caccaccggc gagggaatgg actggggcgt cctctttggc 1260 ttcggccccg gcctcaccgt cgagaccgtt gtcctccaca gcatgcccat tgccgctggt 1320 gccaccgctt gatcgatggt tccatctccg tttatctgcg acatcgataa aaacctacta 1380 ctactactac cgccgccgcc gccgctgcta tccaaagtac tgtaatttgt atcgtattca 1440 tgcatacctg gtttgtattt gttggtagga ttcgttctac tattatgtcg cgtgtgtcgc 1500 gtacaccgtc gtatcctagt agtagtaatc aaacggagta aggtttatat acgtgtcata 1560 atatgggttg tgaggtgcat ttacctgtgt acgagaagat tggctgttta atttcaagct 1620 tatgtggtgg ggaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 1661 34 992 DNA Festuca arundinacea 34 gccgcgccga cctggaagaa gagggcagct tcgacgatgc cgtggctggt tgcgactacg 60 ccttcctcgt cgcagctccg gtgaacctaa aagcagagaa ccccgagaaa gacatggtgg 120 agcctgccgt cggaggaact ctgaacgcga tgaggtcgtg cgtgagagca gggacggtga 180 agcgtgttgt cctgacatcg tcggtggcgt ccgtctccgc ccgtcctctg ctgcaaggcg 240 acggccatgt cctggacgag gagtcctggt ccgacgtcga cttcctcaga gccaaagcga 300 ccggtcactg ggggtaccct gtgtcgaagg tgcttctgga gaaggcggcg tgcgcgttcg 360 cgcaggcgag cggcatcagc ctggtcaccg tgtgccccgt cgtcgtggtg ggcaaggcgc 420 cggcggtgca ggtccacacc agcgtccccg acgtcctctc cccgctatcc ggcgacgaag 480 ccaagatcca aatcctgcag cacatcgaac gggcgtccgg ctccatctcg ttggtccacg 540 tcgacgacct ctgccgcgcc gaggtgttcc tcgccgagga ggaggcggtg gcgtcggggc 600 ggtacatctg ctgcagcctc agcaccaccg ccggcgtgct cgcccgcttc ctctccgtca 660 agtacccgca gtacaaagtc aggaccgacc ggttcagtgg ttcccccgag aagccgagag 720 tgtgcatgtc gtcggcgaag ctcgtcgcgg aagggttcca gtacaagtac aagaccctcg 780 acgagatata cgatgatgtc gtcgagtatg gcagggcctt gggaatcctt ccataatgat 840 acgcgaacgc gacgaggcac tccatgtctc catctatctc tacaactcag gatcaagtga 900 tgcacttgca ataagcctat cctcttatct ctcgatatta atatattttc cttatcaaaa 960 aataaatcta acctcctcct aaaaaaaaaa aa 992 35 1279 DNA Festuca arundinacea 35 gaaaagttag ctttccgaat aaaattatcc tagtgcgtgc agtgcgaacg acactttagc 60 tcgcgcgggc aggaacccac cgacgggcga tacgatggcg gccgcaggtg atgggagcag 120 gaggaagacg gcgtgcgtca ccggagggaa cgggtacatc gcgtcggcgc tcgtgaagat 180 gctgctggag aagggatacg ccgtgaagac gaccgtcaga aacccagatg acatggagaa 240 gaactcccac ctcaaggatt tgcaagcgct gggccccttg gaggtgttcc gcgccgacct 300 gcaagaagag ggcagcttcg acgacgccgt tgccggctgc gactacgcct tcctcgtcgc 360 cgctccggtc aacctcaaag cagagaaccc cgagaaagac atggttgagc cagccgttgg 420 aggaactctg aacgtgatga ggtcgtgcgt gagagcaggg acggtgaagc gtgttgtcct 480 gacatcgtcg gtggcgtccg tctccgcccg tcctctgctg caaggcgacg gccatgtcct 540 ggacgaggag tcctggtccg acgtcgactt cctcagagcc aaagcgaccg gtcactgggg 600 gtaccctgtg tcgaaggtgc ttctggagaa ggcggcgtgc gcgttcgcgc aggcgagcgg 660 catcagcctg gtcaccgtgt gccccgtcgt cgtggtgggc aaggcgccgg cggtgcaggt 720 ccacaccagc gtccccgacg tcctctcccc gctatccggc gacgaagcca agatccaaat 780 cctgcagcac atcgaacggg cgtccggctc catctcgttg gtccacgtcg acgacctctg 840 ccgcgccgag gtgttcctcg ccgaggagga ggcggtggcg tcggggcggt acatctgctg 900 cagcctcagc accaccgccg gcgtgctcgc ccgcttcctc tccgtcaagt acccgcagta 960 caaagtcagg accgaccggt tcagtggttc ccccgagaag ccgagagtgt gcatgtcgtc 1020 ggcgaagctc gtcgcggaag ggttccagta caagtacaag accctcgacg agatatacga 1080 tgatgtcgtc gagtatggca gggccttggg aatccttcca taatgatacg cgaacgcgac 1140 gaggcactcc atgtctccat ctatctctac aactcaggat caagtgatgc acttgcaata 1200 agcctatcct cttatctctc gatattaata tattttcctt atcaaaaaat aaatctaacc 1260 tcctcctaaa aaaaaaaaa 1279 36 1206 DNA Lolium perenne 36 ggtcgcggct ccaatggaca tggggtcact gaatcctgag gtaaacaaca atccagttca 60 tttcatgttt tgggtatgat tcatctcagt tactgtattc tatgtggtgg gactgcgaca 120 gagagatctt gtccaggcag gcgtccaagg aaccctgaac gtgatgaggt cgtgtgtgaa 180 agcggggaca gtgaagcgcg tgatcctgac gtcgtcggat tccgcggtgt gccagaggcc 240 gctggaaggc gacgggcacg tcctggacga gggctcctgg tcggacgtgc cgtacctgcg 300 agcagagcag ccggaggctt gggggtacgc ggtgtcgaag gtgcttatgg aagaggcggc 360 gggcaagttc gcggacgaga acggcctcgg cctcgtcagc gtgctgccca cctttaccct 420 aggcgcggca ccagtgtcgc aggccagaac cagcgtcccc gtcgtcctct ccttgttgtc 480 cggcgacgag gaacagctaa acctcctgga agccatgcac ttgattaccg aatccgtgtc 540 aataaaccac atcgacgacc tctgccgtgc ccaggtgttc ctcgccgaga acgaggcctc 600 atctgggagg tacatctgca gtagccacga caccaccgtc gtgcagctcg cccgtctctt 660 ggcagacaag tacccacaat acaacgtgaa atcccaacgt tttgatgggt cccctgagaa 720 gccaagagtg tgcctctcgt ctcagaagct catcggagaa gggttcgtgt acaagtatga 780 tgacctaggt gccatcttgg acgacctcgt cgagtacggc aggaccacgg ggattcttcc 840 cttctgatat gctcctcctg ttctgccgat cgtatgtatg tgatcggaac gcaacagtgt 900 gtgctttctt cgtcaatggc aggaacaaca cgacagtgtg ctttcttcgt tcttagacag 960 gtctctatgg ctctgaagat tggggatctg atctcttgtt gttttttgcc ccgtagtgtg 1020 gtcttgacga caaggccaca ggcgggtttt cctaccaaat gcttcccttc ttcccagttc 1080 ccttttaatt gctgttaaag agacaaagta ctcctgtatt actacttgat tgatgactct 1140 ggtctctgga acaaagtggg aagatctaga tggaaagagt aatattatca aattttaaaa 1200 aaaaaa 1206 37 1463 DNA Lolium perenne 37 aaaaagtgcc tcgagtcaac tttccaattc ctgagcagag gtagtgactt gagtagttca 60 gttagcagcg ggcagcgatc gatggcgtcc gcagctggag gcaggaggaa gacggcctgc 120 gtcaccggag ggagcggcta catcgcctca gcgctcatca agacgctcct cgatcacggc 180 tacgccgtca agacgaccgt cagaaacccc gatgacctgg agaagacctc ccacctcaag 240 gacttacaag cgtttggccc cttggagatc ttccgtggag agctggatgt ggaaggcagc 300 ttcgacgact cggtttcagg ctgcgactat gtattcctcg tcgcggctcc gatggacatg 360 gggtcactga atcctgagag agatcttgtc caggcaggcg tccaaggaac cctgaacgtg 420 atgaggtcgt gtgtgaaagc ggggacagtg aagcgcgtga tcctgacgtc gtcggattcc 480 gcggtgtgcc agaggccgct ggaaggcgac gggcacgtcc tggacgaggg ctcctggtcg 540 gacgtgccgt acctgcgagc agagcagccg gaggcttggg ggtacgcggt gtcgaaggtg 600 cttatggaag aggcggcggg caagttcgcg gacgagaacg gcctcggcct cgtcagcgtg 660 ctgcccacct ttaccctagg cgcggcacca gtgtcgcagg ccagaaccag cgtccccgtc 720 gtcctctcct tgttgtccgg cgacgaggaa cagctaaacc tcctggaagc catgcacttg 780 attaccgaat ccgtgtcaat aaaccacatc gacgacctct gccgtgccca ggtgttcctc 840 gccgagaacg aggcctcatc tgggaggtac atctgcagta gccacgacac caccgtcgtg 900 cagctcgccc gtctcttggc agacaagtac ccacaataca acgtgaaatc ccaacgtttt 960 gatgggtccc ctgagaagcc aagagtgtgc ctctcgtctc agaagctcat cggagaaggg 1020 ttcgtgtaca agtatgatga cctaggtgcc atcttggacg acctcgtcga gtacggcagg 1080 accacgggga ttcttccctt ctgatatgct cctcctgttc tgccgatcgt atgtatgtga 1140 tcggaacgca acagtgtgtg ctttcttcgt caatggcagg aacaacacga cagtgtgctt 1200 tcttcgttct tagacaggtc tctatggctc tgaagattgg ggatctgatc tcttgttgtt 1260 ttttgccccg tagtgtggtc ttgacgacaa ggccacaggc gggttttcct accaaatgct 1320 tcccttcttc ccagttccct tttaattgct gttaaagaga caaagtactc ctgtattact 1380 acttgattga tgactctggt ctctggaaca aagtgggaag atctagatgg aaagagtaat 1440 attatcaaat tttaaaaaaa aaa 1463 38 1606 DNA Lolium perenne 38 gggcagccgc atccatctgg tttctcttcc gtggcagcag cagcgggaag aagttgtcga 60 agctgccgct gccgccgggg cctcgggggt ggcccgtgct gggcaacctg ccgcaggtgg 120 gcgccaagcc gcaccacacc atggccgctc tctcccaaca gttcggcccg ctcttccgcc 180 tccgcttcgg ggtcgccgag gtggtcgtcg ccgcgtccgc caaggtggcc tcccagttcc 240 tccgcgccca cgacgccaac ttcagcgacc gcccgcccaa ctccggcgcc gagcacgtcg 300 cctacaacta ccaggacctc gtcttcgccc cctacggctc ccgctggcgc gccctccgca 360 agctctgcgc gctccacctc ttctccgcta aggccctcga cgccctccgc gccgtccgcg 420 aggctgaggt tgcgctgatg gtgaagcagc tcaaggagtc ggcgcccgcg ggagtggtgg 480 tggggcagga ggcaaacgtg tgtgccacca acgccctggc gagggcggcc gtggggaggc 540 gcgtgttcgg gggcagcgcc ggagagggcg cacgggagtt caaggacatg gtggtggagc 600 tcatgcagct tgccggggtg ttcaacatcg gcgacttcgt tccggcgctc cgctggctcg 660 acccgcaggg cgttgtggcc aggatgaagc gcctgcaccg ccgctacgac gccatgatgg 720 acggcttcat cagcgagagg gaccagcgtc ataatcaggc tgctgctgac ggggaaagga 780 aggacctgct cagcgtcatg ctggggtaca tgcggccgga cggcggaggc ggcgaggagg 840 aggggatcag cttcaaccac accgacatca aagctcttct cctgaatctg ttcacagctg 900 ggaccgacac gacttctagc acggttgagt gggccctagc tgagctgata cgacacaagg 960 acgtcctcac ccaggcccaa cgcgagctcg atgacatcgt gggccaggat cgcctggtaa 1020 cggaatccga cctaccacac ctcaccttcc tcactgccgt catcaaggag acgttccggc 1080 tgcacccgtc gacgccgctc tcccttcctc gggtggccac tgaggattgt gaggtcgagg 1140 gctaccgcat ccccaagggt accaccttac ttgtcaatgt gtgggccatc gcacgtgacc 1200 cagcctcatg gggccccgat gcgttggagt tcaggcccgc ccgcttcctc gccggcgggc 1260 tgcacgagag tgtggacgtc aaggggagtg actacgagct tataccgttc ggggctggac 1320 gaaggatatg tgcaggcctc agttggggct tgaggatggt cactctcatg accgccacgc 1380 tggtgcatgc atttgactgg tccttagtcg atggccttac cccagaaaaa ctcgacatgg 1440 aggaggcata tggtctcacc cttcagcggg ccgctccgtt aatggttcgg cccattccta 1500 ggttgttatc gtcagcgtac accgtgtgac agatgatgat taatcacttt tgtcgaatgt 1560 atgcaatttg tgcaagtgag ctttacatat gttacaaaaa aaaaaa 1606 39 1708 DNA Lolium perenne 39 gaaagactgg agcacgagga cactgacatg gactgaagga gtagaaaaat tacacatatg 60 gatcatcggg acgtgcttgt gctgctctgc tccttggctg ccctggcagc cgcatccatc 120 tggtttctct tccgtggcag cagcagcgga aagaagttgt cgaagctgcc gctgccgccg 180 gggcctcggg ggtggcccgt gctgggcaac ctgccgcagg tgggcgccaa gccgcaccac 240 accatggccg ctctctccca acagttcggc ccgctcttcc gcctccgctt cggggtcgcc 300 gaggtggtcg tcgccgcgtc cgccaaggtg gcctcccagt tcctccgcgc ccacgacgcc 360 aacttcagcg accgcccgcc caactccggc gccgagcacg tcgcctacaa ctaccaggac 420 ctcgtcttcg ccccctacgg ctcccgctgg cgcgccctcc gcaagctctg cgcgctccac 480 ctcttctccg ctaaggccct cgacgccctc cgcgccgtcc gcgaggctga ggttgcgctg 540 atggtgaagc agctcaagga gtcggcgccc gcgggagtgg tggtggggca ggaggcaaac 600 gtgtgtgcca ccaacgccct ggcgagggcg gccgtgggga ggcgcgtgtt cgggggcagc 660 gccggagagg gcgcacggga gttcaaggac atggtggtgg agctcatgca gcttgccggg 720 gtgttcaaca tcggcgactt cgttccggcg ctccgctggc tcgacccgca gggcgttgtg 780 gccaggatga agcgcctgca ccgccgctac gacgccatga tggacggctt catcagcgag 840 agggaccagc gtcataatca ggctgctgct gacggggaaa ggaaggacct gctcagcgtc 900 atgctggggt acatgcggcc ggacggcgga ggcggcgagg aggaggggat cagcttcaac 960 cacaccgaca tcaaagctct tctcctgaat ctgttcacag ctgggaccga cacgacttct 1020 agcacggttg agtgggccct agctgagctg atacgacaca aggacgtcct cacccaggcc 1080 caacgcgagc tcgatgacat cgtgggccag gatcgcctgg taacggaatc cgacctacca 1140 cacctcacct tcctcactgc cgtcatcaag gagacgttcc ggctgcaccc gtcgacgccg 1200 ctctcccttc ctcgggtggc cactgaggat tgtgaggtcg agggctaccg catccccaag 1260 ggtaccacct tacttgtcaa tgtgtgggcc atcgcacgtg acccagcctc atggggcccc 1320 gatgcgttgg agttcaggcc cgcccgcttc ctcgccggcg ggctgcacga gagtgtggac 1380 gtcaagggga gtgactacga gcttataccg ttcggggctg gacgaaggat atgtgcaggc 1440 ctcagttggg gcttgaggat ggtcactctc atgaccgcca cgctggtgca tgcatttgac 1500 tggtccttag tcgatggcct taccccagaa aaactcgaca tggaggaggc atatggtctc 1560 acccttcagc gggccgctcc gttaatggtt cggcccattc ctaggttgtt atcgtcagcg 1620 tacaccgtgt gacagatgat gattaatcac ttttgtcgaa tgtatgcaat ttgtgcaagt 1680 gagctttaca tatgttacaa aaaaaaaa 1708 40 1747 DNA Festuca arundinacea 40 ggcgtagcga actggccggc atggacatcc cactctcact gctgctctcc actctggcca 60 tctctgcgac catatgctat gtcttcttcc gagccggcaa ggggcaccgt gcgccgctgc 120 cgctgccgcc tggcccgagg ggctggccag tgctggggaa cctcccgcag ctgggcggca 180 agacacacca gaccctgcat gagatgacca aggtgtacgg gcccgtgctc cggctccggt 240 tcggcagctc cgtcgtggtg gtcgccgggt cagccgccgt ggccgagcag ttcctccgca 300 cccacgacgc caagttcagc agccggccgc ccaactccgg cggcgaacac atggcgtaca 360 actacaggga cgtggttttc gcgccctacg gcccccggtg gcgcgcgatg cgcaaggtgt 420 gcgccgtcaa catcttctcg gcccgcgcgc tcgacgatct ccgcggtttc agggagcggg 480 aggccgcgct catggtgcgg tccctcgcgg atgctgccaa agccggggtg gcggtggcgg 540 tcggcaaggc ggcgaacgtg tgcacgacca acggcctgtc tcgggcagcg gtggggctcc 600 gggtgttcgg aagcgatggc gccagagact tcaaggagat cgtgctggag gtgatggagg 660 tgggcggggt tcttaacgtc ggggactttg tgccggcgct ccggtggctc gacccgcagg 720 gtgtcgtcgc gaggttgaag aagctgcacc gccggttcga cgacatgatg aatgggataa 780 tcgccgagag gaggaccgga accaagacgg ccgtggtgga ggaaggtaag ggagacctgc 840 tgggcttgct gcttgcgatg gtgcaggaag acaagtcgct caccggcagc gaggaggaca 900 agatcaccga cactgacgtc aaggcgctta tactgaactt gtttgtggcg ggaacagaga 960 caacgtcgag tatagtggag tgggcagtag cggagctgat caggcaccct gacatcctga 1020 agcaggccca ggaggagcta gatgccgtcg tgggccgtga caggcttgtc tcggagtctg 1080 acctgccacg actcacgttt ttcaatgcca tcatcaagga gacgttccgg ctgcatccgt 1140 cgacgccgct ctcgcttccc cggatggcct ccgaggagtg cgaggtcgcc ggctaccaca 1200 tcccaagggg cactgagcta ctggtcaatg tgtggggcat cgcccgcgat ccggccctat 1260 ggcccgaccc gctggagtac cagcctgccc ggttcctccc aggagggtcg catgagaatg 1320 tcgacctcaa gggaggtgac tttgggctga taccgtttgg ggcgggccgg aggatatgtg 1380 cgggcctaag ctggggcttg cggatggtta ccattacaac cgctaccctg gtgcactcgt 1440 tcgactggga gctgccggcg ggccagacgc cggataagtt gaacatggag gaggccttta 1500 gtctgctgct gcagcgagcc gtgccattga tggtccaccc agtgcccagg ttgcttccat 1560 ccgcatacga aatttcgtag aaaatcgctg cgccagtgat tgtcctgatt gatgatgtat 1620 ggagggcaaa gctccaatta taccatgcac tactatcgat gggttatctc accgtttgaa 1680 ctaaagtagt ttacaatgca tattgttccg agaagttcaa taagaaagaa taacatgaaa 1740 aaaaaaa 1747 41 1763 DNA Festuca arundinacea 41 gaacagttgc cgtgcatgcg tagcgagctg gctggcatgg acatcccact cccactgctg 60 ctctccactc tggccatctc tgcgaccata tgctatgtct tcttccgagc cggcaagggg 120 caccgtgcgc cgctgccgct gccgcctggc ccgaggggct ggccagtgct ggggaacctc 180 ccgcagctgg gcggcaagac acaccagacc ctgcatgaga tgaccaaggt gtacgggccc 240 gtgctccggc tccggttcgg cagctccgtc gtggtggtcg ccgggtcagc cgccgtggcc 300 gagcagttcc tccgcaccca cgacgccaag ttcagcagcc ggccgcccaa ctccggcggc 360 gaacacatgg cgtacaacta cagggacgtg gttttcgcgc cctacggccc ccggtggcgc 420 gcgatgcgca aggtgtgcgc cgtcaacatc ttctcggccc gcgcgctcga cgatctccgc 480 ggtttcaggg agcgggaggc cgcgctcatg gtgcggtccc tcgcggatgc tgccaaagcc 540 ggggtggcgg tggcggtcgg caaggcggcg aacgtgtgca cgaccaacgg cctgtctcgg 600 gcagcggtgg ggctccgggt gttcggaagc gatggcgcca gagacttcaa ggagatcgtg 660 ctggaggtga tggaggtggg cggggttctt aacgtcgggg actttgtgcc ggcgctccgg 720 tggctcgacc cgcagggtgt cgtcgcgagg ttgaagaagc tgcaccgccg gttcgacgac 780 atgatgaatg ggataatcgc cgagaggagg accggaacca agacggccgt ggtggaggaa 840 ggtaagggag acctgctggg cttgctgctt gcgatggtgc aggaagacaa gtcgctcacc 900 ggcagcgagg aggacaagat caccgacact gacgtcaagg cgcttatact gaacttgttt 960 gtggcgggaa cagagacaac gtcgagtata gtggagtggg cagtagcgga gctgatcagg 1020 caccctgaca tcctgaagca ggcccaggag gagctagatg ccgtcgtggg ccgtgacagg 1080 cttgtctcgg agtctgacct gccacgactc acgtttttca atgccatcat caaggagacg 1140 ttccggctgc atccgtcgac gccgctctcg cttccccgga tggcctccga ggagtgcgag 1200 gtcgccggct accacatccc aaggggcact gagctactgg tcaatgtgtg gggcatcgcc 1260 cgcgatccgg ccctatggcc cgacccgctg gagtaccagc ctgcccggtt cctcccagga 1320 gggtcgcatg agaatgtcga cctcaaggga ggtgactttg ggctgatacc gtttggggcg 1380 ggccggagga tatgtgcggg cctaagctgg ggcttgcgga tggttaccat tacaaccgct 1440 accctggtgc actcgttcga ctgggagctg ccggcgggcc agacgccgga taagttgaac 1500 atggaggagg cctttagtct gctgctgcag cgagccgtgc cattgatggt ccacccagtg 1560 cccaggttgc ttccatccgc atacgaaatt tcgtagaaaa tcgctgcgcc agtgattgtc 1620 ctgattgatg atgtatggag ggcaaagctc caattatacc atgcactact atcgatgggt 1680 tatctcaccg tttgaactaa agtagtttac aatgcatatt gttccgagaa gttcaataag 1740 aaagaataac atgaaaaaaa aaa 1763 42 1673 DNA Festuca arundinacea 42 gggacatccc actcccactg ctgctctcca ctctggccat ctctgcgacc atatgctatg 60 tcttcttccg agccggcaag acacaccaga ccctgcatga gatgaccaag gtgtacgggc 120 ccgtgctccg gctccggttc ggcagctccg tggtggtagt ggccggatca gccgccgtgg 180 ccgagcagtt cctgcgcacc cacgacgcca agttcagcag ccggccgccc aactctggcg 240 gcgagcacat ggcttacaac taccaggaca tcgtgttcgc gccctacggg ccccggtggc 300 gcgccatgcg caaggtgtgc gccgtcaaca tcttctcggc ccgcgcgctc gacgatctcc 360 gcgggttcag ggagcgggag gccgcactca tggtgcggtc cctcgcagac gctgccaaag 420 ccggggcggc ggtggcggtc ggcaaggcgg caaacgtgtg cacgaccaac ggcctgtctc 480 gggcggcggt ggggctccgg gtgttcggaa gcgatggcac cagagacttc aaggagatcg 540 tgctggaggt gatggaggtg ggtggggttc ttaatgtcgg ggattttgtg ccggcgctcc 600 ggtggctcga cccacagggg gtcgtcgcga ggatgaagaa gctgcaccgc cggttcgacg 660 acataatgaa cgggataata gccgagagga ggaccggagc caagacggcc gtcgtggagg 720 aaggtaaggg agacctgctg ggcttgctac ttgcgatggt gcaggaagac aagtcgctca 780 ccggcagcga ggaggacaaa atcaccgaca ctgacgtcaa ggcgcttata ctgaacttgt 840 ttgtggcggg aacagagaca acgtcgagca tagtggagtg ggcagtagcg gagctgatca 900 ggcaccctga catcctgaag caggcccagg aggagctaga taccgtcgtg ggccgtgaca 960 ggatcgtctc ggagtcggac ctgccacgac tcaccttttt taatgccatc atcaaggaga 1020 cgttccggct gcatccgtcg acgccgctct cgcttccccg gatggcctcc gaggactgtg 1080 aggtcgctgg ctaccacatc ccaaggggca ccgagctact ggtcaatgtg tggggcatcg 1140 cccgtgaccc atccctatgg cctgacccgc tggagtaccg gcccgcccgg ttcctcccag 1200 gagggtcgca tgagaatgtc gacctcaagg gaggtgactt tgggctgata ccgtttgggg 1260 cgggccggag gatatgtgcg ggcctaagct ggggcttgcg gatggtcacc gttacaaccg 1320 ctaccctggt gcactcgttc gactgggagc tgccggcggg ccagacgctg gataagttga 1380 acatggagga ggcctttagc ctgctgctgc agcgagccat gccattgatg gtccacccgg 1440 tgcccaggtt gcttccatcg gcatacgaaa tttcgtagaa aattgctgcg ccagtgcttg 1500 tcatgattga tgatgtatgg agggcaagct ccaattatac catgcactac tatcgatggg 1560 ttgtctcccc gtttgaacta aagtagttta caatgcatat tgttccgaga agttcaataa 1620 gaaagaataa catggaaaaa tacaatctgt tggacggcca aaaaaaaaaa aaa 1673 43 1714 DNA Festuca arundinacea 43 gaaagaacag ttgccgtgca tgcgtaacga gctggctggc atggacatcc cactcccact 60 gctgctctcc actctggcca tctctgcgac catatgctat gtcttcttcc gagccggcaa 120 gacacaccag accctgcatg agatgaccaa ggtgtacggg cccgtgctcc ggctccggtt 180 cggcagctcc gtggtggtag tggccggatc agccgccgtg gccgagcagt tcctgcgcac 240 ccacgacgcc aagttcagca gccggccgcc caactctggc ggcgagcaca tggcttacaa 300 ctaccaggac atcgtgttcg cgccctacgg gccccggtgg cgcgccatgc gcaaggtgtg 360 cgccgtcaac atcttctcgg cccgcgcgct cgacgatctc cgcgggttca gggagcggga 420 ggccgcactc atggtgcggt ccctcgcaga cgctgccaaa gccggggcgg cggtggcggt 480 cggcaaggcg gcaaacgtgt gcacgaccaa cggcctgtct cgggcggcgg tggggctccg 540 ggtgttcgga agcgatggca ccagagactt caaggagatc gtgctggagg tgatggaggt 600 gggtggggtt cttaatgtcg gggattttgt gccggcgctc cggtggctcg acccacaggg 660 ggtcgtcgcg aggatgaaga agctgcaccg ccggttcgac gacataatga acgggataat 720 agccgagagg aggaccggag ccaagacggc cgtcgtggag gaaggtaagg gagacctgct 780 gggcttgcta cttgcgatgg tgcaggaaga caagtcgctc accggcagcg aggaggacaa 840 aatcaccgac actgacgtca aggcgcttat actgaacttg tttgtggcgg gaacagagac 900 aacgtcgagc atagtggagt gggcagtagc ggagctgatc aggcaccctg acatcctgaa 960 gcaggcccag gaggagctag ataccgtcgt gggccgtgac aggatcgtct cggagtcgga 1020 cctgccacga ctcacctttt ttaatgccat catcaaggag acgttccggc tgcatccgtc 1080 gacgccgctc tcgcttcccc ggatggcctc cgaggactgt gaggtcgctg gctaccacat 1140 cccaaggggc accgagctac tggtcaatgt gtggggcatc gcccgtgacc catccctatg 1200 gcctgacccg ctggagtacc ggcccgcccg gttcctccca ggagggtcgc atgagaatgt 1260 cgacctcaag ggaggtgact ttgggctgat accgtttggg gcgggccgga ggatatgtgc 1320 gggcctaagc tggggcttgc ggatggtcac cgttacaacc gctaccctgg tgcactcgtt 1380 cgactgggag ctgccggcgg gccagacgct ggataagttg aacatggagg aggcctttag 1440 cctgctgctg cagcgagcca tgccattgat ggtccacccg gtgcccaggt tgcttccatc 1500 ggcatacgaa atttcgtaga aaattgctgc gccagtgctt gtcatgattg atgatgtatg 1560 gagggcaagc tccaattata ccatgcacta ctatcgatgg gttgtctccc cgtttgaact 1620 aaagtagttt acaatgcata ttgttccgag aagttcaata agaaagaata acatggaaaa 1680 atacaatctg ttggacggcc aaaaaaaaaa aaaa 1714 44 1449 DNA Lolium perenne 44 gacaaacacc ttaactagat cagctcgatc agcttccagc ttcctctcct agctagctcg 60 ctcgctctta tcgccggtga actgtccccc ggccccggta ctaagctgcg cagggcatgg 120 caatggcgga ctgcatgcag gagtggccgg agcccgtggt gcgcgtgcag gcggtggccg 180 agagcggtct ggccgccatc cccgactgct acgtcaagcc gccgcgcgac cgcccagcgg 240 cgcagcacct ggctaccgcc gcttctgcag atggcgacgt cctccatgag cctctggaca 300 ccagcattcc ggtgatcgac cttggcgagc tcgtcgcggc gacagccgac gagggccgca 360 tgcgccagat catggaggcc gtggcggcgg cgtgccggga gtgggggttc ttccaggtgg 420 tgaaccacgg ggtggcgccg gagctgatgc acgcggcgcg ggaggcgtgg cgcggattct 480 tccggctgcc gatcacggcg aagcagcagt acgccaacct gccgcggacg tacgaggggt 540 acggcagccg agtcggcgtc cagaagggcg gccccctcga ctggggcgac tactatttcc 600 tccacctcgc gccggacgcc ggcaagagcc cggacaagta ctggcccacc aatcccgcca 660 tctgcaagga tgtgtcggag gagtacggtc gtgaggtgat ccggttgtgc gagctgctga 720 tgaaggtgat gtcggcgagc ctgggcctag aggcgacgag gttccaggag gcgttcggcg 780 gatcagagtg cggcgtgtgc cttcgcgcca actactaccc gcggtgcccg cagccggatc 840 tgacgctggg cctgtcggcg cactctgacc cgggcgtcct caccgtgctc ctcgctgacg 900 agcacgtccg cggcctccag gtccgccgcg ccgatggcga gtgggtcacc gtgcagcccg 960 cacggcacga cgccttcatc gtcaacgtcg gcgaccagat ccagatactg agcaactcca 1020 tgtacaagag cgtggagcac cgggtgatgg tgaacgccaa ggaggagcgc atctccctgg 1080 cgctcttcta caacccgcga ggcgacgtcc cgatcgcgcc ggcgccggag acggtgacgc 1140 cggagcggcc ggcgctctac ccgtccatga ccttcgacga gtacagggcc tacatcagga 1200 agtacggccc caggggcaag gcgcaggtcg agggtgccaa gcagggacaa ggtagctagt 1260 tagctggatc cttggagcta gtatctgatc catgggaata attaagccgt ccaggttgta 1320 ccggccaatc tatggattcc tgcatgcatg tacgtgtggc taatgtagca caagctcgcc 1380 cttgtacccg aactgcatat atgctaattg tattggcatc tcgcttagcc gtgccgtcca 1440 aaaaaaaaa 1449 45 473 PRT Lolium perenne 45 Met Ala Ala Ala Ala Val Ala Pro Asp Ala Lys Ile Glu Lys Phe Arg 1 5 10 15 Asp Ala Val Ala Lys Leu Gly Glu Ile Ser Glu Asn Glu Lys Ala Gly 20 25 30 Cys Ile Ser Leu Val Ser Arg Tyr Leu Ser Gly Glu Ala Glu Gln Ile 35 40 45 Glu Trp Ser Lys Ile Gln Thr Pro Thr Asp Glu Val Val Val Pro Tyr 50 55 60 Asp Thr Leu Ala Pro Ala Pro Glu Asp Leu Asp Ala Met Lys Ala Leu 65 70 75 80 Leu Asp Lys Leu Val Val Leu Lys Leu Asn Gly Gly Leu Gly Thr Thr 85 90 95 Met Gly Cys Thr Gly Pro Lys Ser Val Ile Glu Val Arg Asn Gly Phe 100 105 110 Thr Phe Leu Asp Leu Ile Val Ile Gln Ile Glu Ser Leu Asn Lys Lys 115 120 125 Tyr Gly Cys Asp Val Pro Leu Leu Leu Met Asn Ser Phe Asn Thr His 130 135 140 Asp Asp Thr Gln Lys Ile Val Glu Lys Tyr Ser Asn Ser Asn Ile Asn 145 150 155 160 Ile His Thr Phe Asn Gln Ser Gln Tyr Pro Arg Ile Val Thr Glu Asp 165 170 175 Phe Leu Pro Leu Pro Ser Lys Gly Gln Ser Gly Lys Asp Gly Trp Tyr 180 185 190 Pro Pro Gly His Gly Asp Val Phe Pro Ser Leu Asn Asn Ser Gly Lys 195 200 205 Leu Asp Thr Leu Leu Ser Gln Gly Lys Glu Tyr Val Phe Val Ala Asn 210 215 220 Ser Asp Asn Leu Gly Ala Ile Val Asp Ile Lys Ile Leu Asn His Leu 225 230 235 240 Ile Asn Asn Lys Asn Glu Tyr Cys Met Glu Val Thr Pro Lys Thr Leu 245 250 255 Ala Asp Val Lys Gly Gly Thr Leu Ile Ser Tyr Glu Gly Arg Val Gln 260 265 270 Leu Leu Glu Ile Ala Gln Val Pro Asp Glu His Val Asn Glu Phe Lys 275 280 285 Ser Ile Glu Lys Phe Lys Ile Phe Asn Thr Asn Asn Leu Trp Val Asn 290 295 300 Leu Lys Ala Ile Lys Arg Leu Val Glu Ala Asp Ala Leu Lys Met Glu 305 310 315 320 Ile Ile Pro Asn Pro Lys Glu Val Asp Gly Val Lys Val Leu Gln Leu 325 330 335 Glu Thr Ala Ala Gly Ala Ala Ile Arg Phe Phe Asp Asn Ala Ile Gly 340 345 350 Ile Asn Gly Pro Arg Ser Arg Phe Leu Pro Val Lys Ala Thr Ser Asp 355 360 365 Leu Leu Leu Val Gln Ser Asp Leu Tyr Thr Leu Val Asp Gly Tyr Val 370 375 380 Ile Arg Asn Pro Ala Arg Val Lys Pro Ser Asn Pro Ser Ile Glu Leu 385 390 395 400 Gly Pro Glu Phe Lys Lys Val Ala Ser Phe Leu Ala Arg Phe Lys Ser 405 410 415 Ile Pro Ser Ile Val Glu Leu Asp Ser Leu Lys Val Ser Gly Asp Val 420 425 430 Ser Phe Gly Ser Gly Ile Val Leu Lys Gly Asn Val Thr Ile Ala Ala 435 440 445 Lys Ser Gly Val Lys Leu Glu Ile Pro Asp Gly Ala Val Leu Glu Asn 450 455 460 Lys Asp Ile Asn Gly Pro Glu Asp Leu 465 470 46 471 PRT Festuca arundinacea 46 Met Ala Ala Val Ala Ala Asp Ala Lys Ile Glu Lys Phe Arg Asp Ala 1 5 10 15 Val Ala Lys Leu Asp Glu Ile Ser Glu Asn Glu Lys Ala Gly Cys Ile 20 25 30 Ser Leu Val Ser Arg Tyr Leu Ser Gly Glu Ala Glu Gln Ile Glu Trp 35 40 45 Ser Lys Ile Gln Thr Pro Thr Asp Glu Val Val Val Pro Tyr Asp Thr 50 55 60 Leu Ala Pro Ala Pro Gln Asp Leu Asp Ala Met Lys Ala Leu Leu Asp 65 70 75 80 Lys Leu Val Val Leu Lys Leu Asn Gly Gly Leu Gly Thr Thr Met Gly 85 90 95 Cys Thr Gly Pro Lys Ser Val Ile Glu Val Arg Asn Gly Phe Thr Phe 100 105 110 Leu Asp Leu Ile Val Ile Gln Ile Glu Ser Leu Asn Lys Lys Tyr Gly 115 120 125 Cys Asp Val Pro Leu Leu Leu Met Asn Ser Phe Asn Thr His Asp Asp 130 135 140 Thr Gln Lys Ile Val Glu Lys Tyr Ser Asn Ser Asn Ile Asn Ile His 145 150 155 160 Thr Phe Asn Gln Ser Gln Tyr Pro Arg Ile Val Thr Glu Asp Phe Leu 165 170 175 Pro Leu Pro Ser Lys Gly Lys Ser Gly Lys Asp Gly Trp Tyr Pro Pro 180 185 190 Gly His Gly Asp Val Phe Pro Ser Leu Asn Asn Ser Gly Lys Leu Asp 195 200 205 Thr Leu Leu Ser Gln Gly Lys Glu Tyr Val Phe Val Ala Asn Ser Asp 210 215 220 Asn Leu Gly Ala Ile Val Asp Ile Lys Ile Leu Asn His Leu Ile Asn 225 230 235 240 Asn Gln Asn Glu Tyr Cys Met Glu Val Thr Pro Lys Thr Leu Ala Asp 245 250 255 Val Lys Gly Gly Thr Leu Ile Ser Tyr Glu Gly Arg Val Gln Leu Leu 260 265 270 Glu Ile Ala Gln Val Pro Asp Glu His Val Asn Glu Phe Lys Ser Ile 275 280 285 Glu Lys Phe Lys Ile Phe Asn Thr Asn Asn Leu Trp Val Asn Leu Lys 290 295 300 Ala Ile Lys Arg Leu Val Glu Ala Asp Ala Leu Lys Met Glu Ile Ile 305 310 315 320 Pro Asn Pro Lys Glu Val Asp Gly Val Lys Val Leu Gln Leu Glu Thr 325 330 335 Ala Ala Gly Ala Ala Ile Arg Phe Phe Glu Lys Ala Ile Gly Ile Asn 340 345 350 Gly Pro Arg Ser Arg Phe Leu Pro Val Lys Ala Thr Ser Asp Leu Leu 355 360 365 Leu Val Gln Ser Asp Leu Tyr Thr Leu Val Asp Gly Tyr Val Ile Arg 370 375 380 Asn Pro Ala Arg Val Lys Pro Ser Asn Pro Ser Ile Glu Leu Gly Pro 385 390 395 400 Glu Phe Lys Lys Val Ala Ser Phe Leu Ala Arg Phe Lys Ser Ile Pro 405 410 415 Ser Ile Val Glu Leu Asp Ser Leu Lys Val Ser Gly Asp Val Thr Phe 420 425 430 Gly Ser Gly Val Val Leu Lys Gly Asn Val Thr Ile Ala Ala Lys Ser 435 440 445 Gly Val Lys Leu Glu Ile Pro Asp Gly Ala Val Leu Glu Asn Lys Asp 450 455 460 Ile Asn Gly Pro Glu Asp Leu 465 470 47 535 PRT Lolium perenne 47 Cys Leu Arg Arg Arg Thr Tyr Ser Asn Ser Gly Asp Thr His Ala Asp 1 5 10 15 Pro Asn Gly Pro Val Tyr Tyr Gly Gly Trp Tyr His Leu Phe Tyr Gln 20 25 30 His Asn Pro Tyr Gly Asp Ser Trp Gly Asn Val Ser Trp Gly His Ala 35 40 45 Val Ser Lys Asp Leu Val Asn Trp Arg His Leu Pro Val Ala Leu Val 50 55 60 Pro Asp Gln Trp Tyr Asp Ile Asn Gly Val Leu Thr Gly Ser Ile Thr 65 70 75 80 Val Leu Pro Asp Gly Arg Val Ile Leu Leu Tyr Thr Gly Asn Thr Asp 85 90 95 Thr Phe Ser Gln Val Gln Cys Leu Ala Val Pro Ala Asp Pro Ser Asp 100 105 110 Pro Leu Leu Arg Ser Trp Ile Lys His Pro Ala Asn Pro Ile Leu Phe 115 120 125 Pro Pro Pro Gly Ile Gly Leu Lys Asp Phe Arg Asp Pro Leu Thr Ala 130 135 140 Trp Phe Glu His Ser Asp Asn Thr Trp Arg Thr Ile Ile Gly Ser Lys 145 150 155 160 Asp Asp Asp Gly His Ala Gly Ile Val Leu Ser Tyr Lys Thr Thr Asp 165 170 175 Phe Val Asn Tyr Glu Leu Met Pro Gly Asn Met His Arg Gly Pro Asp 180 185 190 Gly Thr Gly Met Tyr Glu Cys Leu Asp Ile Tyr Pro Val Gly Gly Asn 195 200 205 Ser Ser Glu Met Leu Gly Gly Asp Ser Ser Pro Glu Val Leu Phe Val 210 215 220 Leu Lys Glu Ser Ala Asn Asp Glu Trp His Asp Tyr Tyr Ala Leu Gly 225 230 235 240 Trp Phe Asp Ala Thr Ala Asn Thr Trp Thr Pro Gln Asp Pro Glu Ala 245 250 255 Asp Leu Gly Ile Gly Leu Arg Tyr Asp Trp Gly Lys Tyr Tyr Ala Ser 260 265 270 Lys Ser Phe Tyr Asp Pro Ile Lys Asn Arg Arg Val Val Trp Ala Phe 275 280 285 Val Gly Glu Thr Asp Ser Glu Gln Ala Asp Lys Ala Lys Gly Trp Ala 290 295 300 Ser Leu Met Ser Ile Pro Arg Met Val Glu Leu Asp Lys Lys Thr Arg 305 310 315 320 Thr Asn Leu Ile Gln Trp Pro Val Glu Glu Ile Glu Thr Leu Arg Arg 325 330 335 Asn Val Thr Asp Leu Gly Gly Ile Thr Val Glu Ala Gly Ser Val Ile 340 345 350 His Leu Pro Leu Gln Gln Gly Gly Gln Leu Asp Ile Glu Ala Ser Phe 355 360 365 Arg Leu Asn Ser Ser Asp Ile Asp Ala Leu Asn Glu Ala Asp Val Gly 370 375 380 Phe Asn Cys Ser Ser Ser Ala Gly Ala Ala Val Arg Gly Ala Leu Gly 385 390 395 400 Pro Phe Gly Leu Leu Val Phe Ala Asp Gly Arg His Glu Gln Thr Ala 405 410 415 Ala Tyr Phe Tyr Val Ser Lys Gly Leu Asp Gly Ser Leu Leu Thr His 420 425 430 Tyr Cys His Asp Glu Ser Arg Ser Thr Arg Ala Lys Asp Val Val Ser 435 440 445 Arg Val Val Gly Gly Thr Val Pro Val Leu Asp Gly Glu Thr Phe Ser 450 455 460 Val Arg Val Leu Val Asp His Ser Ile Val Gln Ser Phe Val Met Gly 465 470 475 480 Gly Arg Thr Thr Val Thr Ser Arg Ala Tyr Pro Thr Glu Ala Ile Tyr 485 490 495 Ala Ala Ala Gly Val Tyr Leu Phe Asn Asn Ala Thr Ser Ala Thr Ile 500 505 510 Thr Ala Glu Gly Leu Val Val Tyr Glu Met Ala Ser Ala Glu Ser Gln 515 520 525 Ala Phe Leu Ala Asp Asp Met 530 535 48 637 PRT Lolium perenne 48 Met Glu Ser Ser Ala Val Val Val Pro Gly Thr Thr Ala Pro Leu Leu 1 5 10 15 Pro Tyr Asp Ser Arg Glu Asn Gln Ser Ser Gly Gly Gly Val Trp Trp 20 25 30 Arg Ala Cys Ala Ala Ser Ala Val Val Leu Leu Val Val Val Gly Phe 35 40 45 Phe Ala Gly Gly Arg Val Asp Leu Gly Gln Ala Gly Glu Val Ser Ala 50 55 60 Thr Ser Ser Val Pro Ala Ala Met Met Glu Ile Pro Arg Ser Arg Gly 65 70 75 80 Lys Asn Phe Gly Val Ser Glu Lys Ala Asp Gly Gly Phe Pro Trp Ser 85 90 95 Asn Ala Met Leu Gln Trp Gln His Thr Gly Phe His Phe Gln Pro Leu 100 105 110 Lys His Tyr Met Asn Asp Pro Asn Gly Pro Val Tyr Tyr Gly Gly Trp 115 120 125 Tyr His Leu Phe Tyr Gln His Asn Pro Tyr Gly Asp Ser Trp Gly Asn 130 135 140 Val Ser Trp Gly His Ala Val Ser Lys Asp Leu Val Asn Trp Arg His 145 150 155 160 Leu Pro Val Ala Leu Val Pro Asp Gln Trp Tyr Asp Ile Asn Gly Val 165 170 175 Leu Thr Gly Ser Ile Thr Val Leu Pro Asp Gly Arg Val Ile Leu Leu 180 185 190 Tyr Thr Gly Asn Thr Asp Thr Phe Ser Gln Val Gln Cys Leu Ala Val 195 200 205 Pro Ala Asp Pro Ser Asp Pro Leu Leu Arg Ser Trp Ile Lys His Pro 210 215 220 Ala Asn Pro Ile Leu Phe Pro Pro Pro Gly Ile Gly Leu Lys Asp Phe 225 230 235 240 Arg Asp Pro Leu Thr Ala Trp Phe Glu His Ser Asp Asn Thr Trp Arg 245 250 255 Thr Ile Ile Gly Ser Lys Asp Asp Asp Gly His Ala Gly Ile Val Leu 260 265 270 Ser Tyr Lys Thr Thr Asp Phe Val Asn Tyr Glu Leu Met Pro Gly Asn 275 280 285 Met His Arg Gly Pro Asp Gly Thr Gly Met Tyr Glu Cys Leu Asp Ile 290 295 300 Tyr Pro Val Gly Gly Asn Ser Ser Glu Met Leu Gly Gly Asp Ser Ser 305 310 315 320 Pro Glu Val Leu Phe Val Leu Lys Glu Ser Ala Asn Asp Glu Trp His 325 330 335 Asp Tyr Tyr Ala Leu Gly Trp Phe Asp Ala Thr Ala Asn Thr Trp Thr 340 345 350 Pro Gln Asp Pro Glu Ala Asp Leu Gly Ile Gly Leu Arg Tyr Asp Trp 355 360 365 Gly Lys Tyr Tyr Ala Ser Lys Ser Phe Tyr Asp Pro Ile Lys Asn Arg 370 375 380 Arg Val Val Trp Ala Phe Val Gly Glu Thr Asp Ser Glu Gln Ala Asp 385 390 395 400 Lys Ala Lys Gly Trp Ala Ser Leu Met Ser Ile Pro Arg Met Val Glu 405 410 415 Leu Asp Lys Lys Thr Arg Thr Asn Leu Ile Gln Trp Pro Val Glu Glu 420 425 430 Ile Glu Thr Leu Arg Arg Asn Val Thr Asp Leu Gly Gly Ile Thr Val 435 440 445 Glu Ala Gly Ser Val Ile His Leu Pro Leu Gln Gln Gly Gly Gln Leu 450 455 460 Asp Ile Glu Ala Ser Phe Arg Leu Asn Ser Ser Asp Ile Asp Ala Leu 465 470 475 480 Asn Glu Ala Asp Val Gly Phe Asn Cys Ser Ser Ser Ala Gly Ala Ala 485 490 495 Val Arg Gly Ala Leu Gly Pro Phe Gly Leu Leu Val Phe Ala Asp Gly 500 505 510 Arg His Glu Gln Thr Ala Ala Tyr Phe Tyr Val Ser Lys Gly Leu Asp 515 520 525 Gly Ser Leu Leu Thr His Tyr Cys His Asp Glu Ser Arg Ser Thr Arg 530 535 540 Ala Lys Asp Val Val Ser Arg Val Val Gly Gly Thr Val Pro Val Leu 545 550 555 560 Asp Gly Glu Thr Phe Ser Val Arg Val Leu Val Asp His Ser Ile Val 565 570 575 Gln Ser Phe Val Met Gly Gly Arg Thr Thr Val Thr Ser Arg Ala Tyr 580 585 590 Pro Thr Glu Ala Ile Tyr Ala Ala Ala Gly Val Tyr Leu Phe Asn Asn 595 600 605 Ala Thr Ser Ala Thr Ile Thr Ala Glu Gly Leu Val Val Tyr Glu Met 610 615 620 Ala Ser Ala Glu Ser Gln Ala Phe Leu Ala Asp Asp Met 625 630 635 49 603 PRT Lolium perenne 49 Met Gly Ile Ala Glu Val Ala Leu His Thr Met Pro Gly Ala Phe Ala 1 5 10 15 Ser His Ser Pro Ala Ser Ser Leu Pro Leu Arg Thr Asp Thr Arg Ser 20 25 30 Leu Arg Lys Arg Gly Thr Asn Ser Phe Tyr Arg Thr Leu Gly Gly Pro 35 40 45 Pro Lys Phe Pro Glu Leu Arg Pro Val Glu Cys Gln Cys Gln Arg Ile 50 55 60 Asp Asp Leu Ala Gly Val Ile Glu Ala Gly Asn Gly Thr Trp Ala Thr 65 70 75 80 Asp Met Val Asn Lys Ala Ser Gln Val Leu Gly Asp Val Ala Val Pro 85 90 95 Gly Gln Ala Leu Gly Gly Asn Ala Ser Leu Ser Gly Asp Pro Glu Lys 100 105 110 Val Leu Pro Arg Arg Arg Asn Leu Ser Ser Val Glu Asp Glu Ala Trp 115 120 125 Asp Leu Leu Arg Glu Ser Val Val Asn Tyr Cys Gly Ser Pro Val Gly 130 135 140 Thr Ile Ala Ala Asn Asp Pro Asn Asp Ser Asn Pro Ala Asn Tyr Asp 145 150 155 160 Gln Val Phe Ile Arg Asp Phe Ile Pro Ser Gly Ile Ala Phe Leu Leu 165 170 175 Lys Gly Glu Tyr Glu Ile Val Arg Asn Phe Ile Leu His Thr Leu Gln 180 185 190 Leu Gln Ser Trp Glu Lys Thr Met Asp Cys His Ser Pro Gly Gln Gly 195 200 205 Leu Met Pro Ala Ser Phe Lys Val Arg Thr Ile Pro Leu Asp Gly Asp 210 215 220 Glu Asn Ala Thr Glu Glu Val Leu Asp Pro Asp Phe Gly Glu Ala Ala 225 230 235 240 Ile Gly Arg Val Ala Pro Val Asp Ser Gly Leu Trp Trp Ile Ile Leu 245 250 255 Leu Arg Ala Tyr Gly Lys Cys Ser Gly Asp Leu Ser Val Gln Glu Arg 260 265 270 Ile Asp Val Gln Thr Gly Ile Lys Met Ile Leu Lys Leu Cys Leu Ala 275 280 285 Asp Gly Phe Asp Met Phe Pro Thr Leu Leu Val Thr Asp Gly Ser Cys 290 295 300 Met Ile Asp Arg Arg Met Gly Ile His Gly His Pro Leu Glu Ile Gln 305 310 315 320 Ala Leu Phe Tyr Ser Ala Leu Leu Ser Ala Arg Glu Met Leu Thr Pro 325 330 335 Glu Asp Gly Ser Ala Asp Leu Ile Arg Ala Leu Asn Asn Arg Leu Val 340 345 350 Ala Leu Ser Phe His Ile Arg Glu Tyr Tyr Trp Val Asp Met Gln Lys 355 360 365 Leu Asn Glu Ile Tyr Arg Tyr Lys Thr Glu Glu Tyr Ser Tyr Asp Ala 370 375 380 Val Asn Lys Phe Asn Ile Tyr Pro Asp Gln Val Ser Pro Trp Leu Val 385 390 395 400 Glu Trp Ile Pro Pro Lys Gly Gly Tyr Phe Ile Gly Asn Leu Gln Pro 405 410 415 Ala His Met Asp Phe Arg Phe Phe Ser Leu Gly Asn Leu Trp Ser Ile 420 425 430 Val Ser Ser Leu Ala Thr Thr Gln Gln Ser His Ala Ile Leu Asp Leu 435 440 445 Ile Glu Ser Lys Trp Ser Asp Leu Val Ala Glu Met Pro Leu Lys Ile 450 455 460 Cys Tyr Pro Ala Leu Glu Asn Leu Glu Trp Lys Ile Ile Thr Gly Ser 465 470 475 480 Asp Pro Lys Asn Thr Pro Trp Ser Tyr His Asn Gly Gly Ser Trp Pro 485 490 495 Thr Leu Leu Trp Gln Leu Thr Val Ala Ser Leu Lys Met Asn Arg Pro 500 505 510 Glu Ile Ala Ala Lys Ala Val Glu Ile Ala Glu Arg Arg Ile Ala Thr 515 520 525 Asp Lys Trp Pro Glu Tyr Tyr Asp Thr Lys Arg Ala Arg Phe Ile Gly 530 535 540 Lys Gln Ser Arg Leu Tyr Gln Thr Trp Ser Ile Ala Gly Tyr Leu Val 545 550 555 560 Ala Lys Gln Leu Leu Asp Lys Pro Asp Ala Ala Arg Ile Leu Trp Asn 565 570 575 Asp Glu Asp Thr Glu Ile Leu Asn Ala Phe Ser Thr Asn Arg Lys Arg 580 585 590 Gly Lys Lys Val Leu Lys Lys Thr Tyr Ile Val 595 600 50 556 PRT Festuca arundinacea 50 Asp Pro Phe Arg Ala Ala Leu Ala Pro Ala Ser Pro Pro Leu Glu Ala 1 5 10 15 Pro Pro Leu Asp Glu Leu Pro Thr Ala Pro Ser His Ser Glu Pro Ala 20 25 30 Ser Ala Ala Ala Ala Ala Pro Glu Gln Asp Pro Val Asp Leu Gln His 35 40 45 Glu Glu Leu Asp Gly Leu Lys Ala Gly Val Glu Ala Val Arg Ser Arg 50 55 60 Glu Glu Ser Pro Gln Glu Lys Glu Ala Trp Trp Leu Leu Asn Arg Ala 65 70 75 80 Val Val Asn Tyr Cys Gly Ser Ala Val Gly Thr Val Ala Ala Asn Asp 85 90 95 Pro Ser Thr Ala Asn His Met Leu Asn Tyr Asp Gln Val Phe Ile Arg 100 105 110 Asp Phe Val Pro Ser Ala Ile Ala Phe Leu Leu Lys Gly Glu Ser Asp 115 120 125 Ile Val Lys Asn Phe Leu Leu His Thr Leu Gln Leu Gln Ser Trp Glu 130 135 140 Lys Thr Val Asp Cys Tyr Ser Pro Gly Gln Gly Leu Met Pro Ala Ser 145 150 155 160 Phe Lys Val Arg Ser Val Pro Leu Asp Gly Asn Asn Glu Ala Phe Glu 165 170 175 Glu Val Leu Asp Pro Asp Phe Gly Glu Ser Ala Ile Gly Arg Val Ala 180 185 190 Pro Val Asp Ser Gly Leu Trp Trp Ile Ile Leu Leu Arg Ala Tyr Gly 195 200 205 Lys Ile Thr Gly Asp Tyr Ala Leu Gln Glu Arg Val Asp Val Gln Thr 210 215 220 Gly Ile Arg Leu Ile Leu Asn Leu Cys Leu Ser Asp Gly Phe Asp Met 225 230 235 240 Phe Pro Thr Leu Leu Val Thr Asp Gly Ser Cys Met Ile Asp Arg Arg 245 250 255 Met Gly Ile His Gly His Pro Leu Glu Ile Gln Ala Leu Phe Tyr Ser 260 265 270 Ala Leu Arg Cys Ala Arg Glu Met Val Asn Ile Asp Asp Gly Ser Lys 275 280 285 Asn Leu Ile Arg Val Ile Asn Asn Arg Leu Ser Ala Leu Ser Phe His 290 295 300 Ile Arg Glu Tyr Tyr Trp Val Asp Met Lys Lys Ile Asn Glu Ile Tyr 305 310 315 320 Arg Tyr Lys Thr Glu Glu Tyr Ser His Asp Ala Ile Asn Lys Phe Asn 325 330 335 Ile Tyr Pro Glu Gln Ile Pro Ser Trp Leu Ala Asp Trp Ile Pro Glu 340 345 350 Lys Gly Gly Tyr Leu Ile Gly Asn Leu Gln Pro Ala His Met Asp Phe 355 360 365 Arg Phe Phe Ser Leu Gly Asn Leu Trp Ala Ile Val Ser Ser Leu Ala 370 375 380 Thr Pro Lys Gln Ala Glu Gly Ile Leu Asn Leu Ile Glu Thr Lys Trp 385 390 395 400 Asp Asp Ile Val Ala Asn Met Pro Leu Lys Ile Cys Tyr Pro Ala Leu 405 410 415 Glu Tyr Glu Glu Trp Arg Ile Ile Thr Gly Cys Asp Pro Lys Asn Thr 420 425 430 Pro Trp Ser Tyr His Asn Gly Gly Ser Trp Pro Thr Leu Leu Trp Gln 435 440 445 Phe Thr Leu Ala Cys Ile Lys Met Gly Arg Pro Asp Leu Ala Arg Arg 450 455 460 Ala Val Glu Ala Val Glu Lys Arg Leu Ser Asp Asp Lys Trp Pro Glu 465 470 475 480 Tyr Tyr Asp Thr Arg Asn Gly Arg Phe Ile Gly Lys Gln Ser Arg Leu 485 490 495 Tyr Gln Thr Trp Thr Ile Ala Gly Phe Leu Ser Ser Lys Leu Leu Leu 500 505 510 Asp Cys Pro Glu Met Ala Ser Ile Leu Ile Cys Asp Glu Asp Leu Asp 515 520 525 Leu Leu Glu Gly Cys Ala Cys Gly Ala Asn Lys Ser Ala Arg Val Lys 530 535 540 Cys Ser Arg Arg Ala Ala Arg Ser Gln Val Leu Val 545 550 555 51 621 PRT Festuca arundinacea 51 Met Ala Ala Ala Ala Ile Ser His Leu Arg Arg Gly Thr Gln Arg His 1 5 10 15 Ala Leu Leu Tyr Leu Ser Arg Arg His Phe Ser Asn Ser Pro Leu Thr 20 25 30 Ala Ala Ala Pro Leu Ala Ala Ala Ala Arg Arg Leu Leu Ser Thr Thr 35 40 45 Val Glu Ser Gly Thr Ser Ser Ala Ala Gly Ser Tyr Lys Pro Pro Pro 50 55 60 Leu Asp Pro Phe Arg Ala Ala Leu Ala Pro Ala Ser Pro Pro Leu Glu 65 70 75 80 Ser Pro Pro Leu Asp Glu Leu Pro Thr Ala Pro Ser His Ser Glu Pro 85 90 95 Ala Ser Ala Ala Ala Ala Ala Pro Glu Gln Asp Pro Val Asp Leu Gln 100 105 110 His Glu Glu Leu Asp Gly Leu Lys Ala Gly Val Glu Ala Val Arg Ser 115 120 125 Arg Glu Glu Ser Pro Gln Glu Lys Glu Ala Trp Trp Leu Leu Asn Arg 130 135 140 Ala Val Val Asn Tyr Cys Gly Ser Ala Val Gly Thr Val Ala Ala Asn 145 150 155 160 Asp Pro Ser Thr Ala Asn His Met Leu Asn Tyr Asp Gln Val Phe Ile 165 170 175 Arg Asp Phe Val Pro Ser Ala Ile Ala Phe Leu Leu Lys Gly Glu Ser 180 185 190 Asp Ile Val Lys Asn Phe Leu Leu His Thr Leu Gln Leu Gln Ser Trp 195 200 205 Glu Lys Thr Val Asp Cys Tyr Ser Pro Gly Gln Gly Leu Met Pro Ala 210 215 220 Ser Phe Lys Val Arg Ser Val Pro Leu Asp Gly Asn Asn Glu Ala Phe 225 230 235 240 Glu Glu Val Leu Asp Pro Asp Phe Gly Glu Ser Ala Ile Gly Arg Val 245 250 255 Ala Pro Val Asp Ser Gly Leu Trp Trp Ile Ile Leu Leu Arg Ala Tyr 260 265 270 Gly Lys Ile Thr Gly Asp Tyr Ala Leu Gln Glu Arg Val Asp Val Gln 275 280 285 Thr Gly Ile Arg Leu Ile Leu Asn Leu Cys Leu Ser Asp Gly Phe Asp 290 295 300 Met Phe Pro Thr Leu Leu Val Thr Asp Gly Ser Cys Met Ile Asp Arg 305 310 315 320 Arg Met Gly Ile His Gly His Pro Leu Glu Ile Gln Ala Leu Phe Tyr 325 330 335 Ser Ala Leu Arg Cys Ala Arg Glu Met Val Asn Ile Asp Asp Gly Ser 340 345 350 Lys Asn Leu Ile Arg Val Ile Asn Asn Arg Leu Ser Ala Leu Ser Phe 355 360 365 His Ile Arg Glu Tyr Tyr Trp Val Asp Met Lys Lys Ile Asn Glu Ile 370 375 380 Tyr Arg Tyr Lys Thr Glu Glu Tyr Ser His Asp Ala Ile Asn Lys Phe 385 390 395 400 Asn Ile Tyr Pro Glu Gln Ile Pro Ser Trp Leu Ala Asp Trp Ile Pro 405 410 415 Glu Lys Gly Gly Tyr Leu Ile Gly Asn Leu Gln Pro Ala His Met Asp 420 425 430 Phe Arg Phe Phe Ser Leu Gly Asn Leu Trp Ala Ile Val Ser Ser Leu 435 440 445 Ala Thr Pro Lys Gln Ala Glu Gly Ile Leu Asn Leu Ile Glu Thr Lys 450 455 460 Trp Asp Asp Ile Val Ala Asn Met Pro Leu Lys Ile Cys Tyr Pro Ala 465 470 475 480 Leu Glu Tyr Glu Glu Trp Arg Ile Ile Thr Gly Cys Asp Pro Lys Asn 485 490 495 Thr Pro Trp Ser Tyr His Asn Gly Gly Ser Trp Pro Thr Leu Leu Trp 500 505 510 Gln Phe Thr Leu Ala Cys Ile Lys Met Gly Arg Pro Asp Leu Ala Arg 515 520 525 Arg Ala Val Glu Ala Val Glu Lys Arg Leu Ser Asp Asp Lys Trp Pro 530 535 540 Glu Tyr Tyr Asp Thr Arg Asn Gly Arg Phe Ile Gly Lys Gln Ser Arg 545 550 555 560 Leu Tyr Gln Thr Trp Thr Ile Ala Gly Phe Leu Ser Ser Lys Leu Leu 565 570 575 Leu Asp Cys Pro Glu Met Ala Ser Ile Leu Ile Cys Asp Glu Asp Leu 580 585 590 Asp Leu Leu Glu Gly Cys Ala Cys Gly Ala Asn Lys Ser Ala Arg Val 595 600 605 Lys Cys Ser Arg Arg Ala Ala Arg Ser Gln Val Leu Val 610 615 620 52 244 PRT Lolium perenne 52 Leu Leu Glu Lys Arg Lys Leu Asn Glu Ile Tyr Arg Tyr Lys Thr Glu 1 5 10 15 Glu Tyr Ser Tyr Asp Ala Val Asn Lys Phe Asn Ile Tyr Pro Asp Gln 20 25 30 Ile Pro Pro Trp Leu Val Glu Trp Ile Pro Pro Lys Gly Gly Tyr Phe 35 40 45 Ile Gly Asn Leu Gln Pro Ala His Met Asp Phe Arg Phe Phe Ser Leu 50 55 60 Gly Asn Leu Trp Ser Ile Val Ser Ser Leu Ala Thr Ala Asp Gln Ser 65 70 75 80 His Ala Ile Leu Asp Leu Val Glu Ala Lys Trp Ser Asp Leu Val Ala 85 90 95 Glu Met Pro Met Lys Ile Cys Tyr Pro Ala Leu Glu Asp Gln Glu Trp 100 105 110 Lys Phe Ile Thr Gly Ser Asp Pro Lys Asn Thr Pro Trp Ser Tyr His 115 120 125 Asn Gly Gly Ser Trp Pro Thr Leu Leu Trp Gln Leu Thr Val Ala Cys 130 135 140 Ile Lys Met Asn Arg Pro Glu Ile Ala Ala Arg Ala Val Glu Val Ala 145 150 155 160 Glu Ser Arg Ile Ser Met Asp Lys Trp Pro Glu Tyr Tyr Asp Thr Lys 165 170 175 Arg Gly Arg Phe Ile Gly Lys Gln Ala Arg Leu Phe Gln Thr Trp Ser 180 185 190 Ile Ala Gly Phe Leu Val Ala Lys Leu Leu Leu Glu Asn Pro Glu Lys 195 200 205 Ser Arg Ile Leu Trp Asn Asn Glu Asp Glu Glu Ile Leu Asn Ala Leu 210 215 220 Ser Leu Met Thr Gly Pro Ser Ser Pro Lys Arg Lys Arg Gly Arg Lys 225 230 235 240 Thr Tyr Ile Val 53 578 PRT Lolium perenne 53 Met Ala Ile Ala Ala Ala Ala Ala Leu Leu Pro Leu His Leu Gly Cys 1 5 10 15 Ser Asp Ala Ala Pro Arg Arg Pro Gly Asn Ser Leu Arg Ala His Leu 20 25 30 Arg Lys Gly Gly Ile Arg Gly Arg Arg Arg Ser Pro Pro Cys Ala Val 35 40 45 Asn Ser Leu His Pro Ser Gly Asn Pro Lys Thr Pro Gly Gly Gly Asp 50 55 60 Val Gly Gly Ala Trp Gly Leu Asn Gly Gly Ala Thr Ala Lys Pro Asp 65 70 75 80 His Ala Pro Pro Ser Gln Arg Arg Arg Ala Pro Arg Asp Val Glu Glu 85 90 95 Glu Ala Trp Ala Leu Leu Arg Glu Ser Val Val Ser Tyr Cys Gly Ser 100 105 110 Pro Val Gly Thr Ile Ala Ala Cys Asp Pro Asn Asp Ala Ser Pro Leu 115 120 125 Asn Tyr Asp Gln Val Phe Ile Arg Asp Phe Val Pro Ser Gly Val Ala 130 135 140 Phe Leu Leu Lys Gly Glu His Glu Ile Val Arg Asn Phe Ile Leu His 145 150 155 160 Thr Leu Gln Leu Gln Ser Trp Glu Lys Thr Ile Asp Cys His Ser Pro 165 170 175 Gly Gln Gly Leu Met Pro Ala Ser Phe Lys Val Arg Val Val Pro Leu 180 185 190 Asp Gly Gly Asp Asp Gly Ala Thr Glu Glu Val Leu Asp Pro Asp Phe 195 200 205 Gly Glu Ala Ala Ile Gly Arg Val Ala Pro Val Asp Ser Gly Leu Trp 210 215 220 Trp Ile Ile Leu Leu Arg Ala Tyr Gly Lys Cys Ser Gly Asp Leu Ser 225 230 235 240 Phe His Glu Arg Val Asp Val Gln Thr Gly Ile Lys Leu Ile Leu Lys 245 250 255 Leu Cys Leu Ala Asp Gly Phe Asp Met Phe Pro Thr Leu Leu Val Thr 260 265 270 Asp Gly Ser Cys Met Met Asp Arg Arg Met Gly Ile His Gly His Pro 275 280 285 Leu Glu Ile Gln Ala Leu Phe Tyr Ser Ala Leu Leu Ser Ala Arg Glu 290 295 300 Met Leu Thr Pro Glu Asp Gly Ser Ala Asp Leu Ile Arg Ala Leu Asn 305 310 315 320 Ser Arg Leu Met Ala Leu Ser Phe His Ile Arg Glu Tyr Tyr Trp Leu 325 330 335 Glu Lys Arg Lys Leu Asn Glu Ile Tyr Arg Tyr Lys Thr Glu Glu Tyr 340 345 350 Ser Tyr Asp Ala Val Asn Lys Phe Asn Ile Tyr Pro Asp Gln Ile Pro 355 360 365 Pro Trp Leu Val Glu Trp Ile Pro Pro Lys Gly Gly Tyr Phe Ile Gly 370 375 380 Asn Leu Gln Pro Ala His Met Asp Phe Arg Phe Phe Ser Leu Gly Asn 385 390 395 400 Leu Trp Ser Ile Val Ser Ser Leu Ala Thr Ala Asp Gln Ser His Ala 405 410 415 Ile Leu Asp Leu Val Glu Ala Lys Trp Ser Asp Leu Val Ala Glu Met 420 425 430 Pro Met Lys Ile Cys Tyr Pro Ala Leu Glu Asp Gln Glu Trp Lys Phe 435 440 445 Ile Thr Gly Ser Asp Pro Lys Asn Thr Pro Trp Ser Tyr His Asn Gly 450 455 460 Gly Ser Trp Pro Thr Leu Leu Trp Gln Leu Thr Val Ala Cys Ile Lys 465 470 475 480 Met Asn Arg Pro Glu Ile Ala Ala Arg Ala Val Glu Val Ala Glu Ser 485 490 495 Arg Ile Ser Met Asp Lys Trp Pro Glu Tyr Tyr Asp Thr Lys Arg Gly 500 505 510 Arg Phe Ile Gly Lys Gln Ala Arg Leu Phe Gln Thr Trp Ser Ile Ala 515 520 525 Gly Phe Leu Val Ala Lys Leu Leu Leu Glu Asn Pro Glu Lys Ser Arg 530 535 540 Ile Leu Trp Asn Asn Glu Asp Glu Glu Ile Leu Asn Ala Leu Ser Leu 545 550 555 560 Met Thr Gly Pro Ser Ser Pro Lys Arg Lys Arg Gly Arg Lys Thr Tyr 565 570 575 Ile Val 54 619 PRT Lolium perenne 54 Met Asn Gly Gln Thr Thr Met Gly Leu Ala Ala Ala Ala Ala Ala Ala 1 5 10 15 Val Arg Pro Cys Arg Arg Arg Leu Leu Ser Ser Ala Ser Ala Ala Ala 20 25 30 Ala Ala Lys Ala Ser Ala Thr Pro Leu Phe Pro Arg Cys Ser His Pro 35 40 45 Gln His Gln Gln His Ser Arg Arg Ile Pro Phe Leu Val Ser Ala Ala 50 55 60 Ser His Thr Ser Gln Ser Asp Pro Ser Thr Thr Pro Thr Pro Val Thr 65 70 75 80 Ser Asp Pro Arg Ser Ala Val Ala Gly Asn Leu Pro Phe Phe Asp Arg 85 90 95 Val Leu Phe Pro Gly Ser Phe Pro Leu Glu Thr Pro Pro Val Glu Glu 100 105 110 Pro Ala Pro Ala Pro Pro Ala Asp Glu Ala Gln Ala Ser Ala Ser Pro 115 120 125 Val Arg Glu Glu Ser Asp Thr Glu Arg Glu Ala Trp Arg Leu Leu Arg 130 135 140 Arg Ala Val Val Ser Tyr Cys Gly Asp Pro Val Gly Thr Val Ala Ala 145 150 155 160 Glu Asp Pro Glu Cys Thr Glu Met Leu Asn Tyr Asp Gln Val Phe Ile 165 170 175 Arg Asp Phe Val Pro Ser Ala Leu Ala Phe Leu Met Arg Gly Glu Thr 180 185 190 Glu Ile Val Arg Asn Phe Leu Leu His Thr Leu Gln Leu Gln Ser Trp 195 200 205 Glu Lys Thr Val Asp Cys Tyr Ser Pro Gly Gln Gly Leu Met Pro Ala 210 215 220 Ser Phe Lys Ile Lys Thr Val Pro Leu Asp Glu Asn Asn Glu Ala Phe 225 230 235 240 Glu Glu Val Leu Asp Pro Asp Phe Gly Glu Ser Ala Ile Gly Arg Val 245 250 255 Ala Pro Val Asp Ser Gly Leu Trp Trp Ile Ile Leu Leu Arg Ala Tyr 260 265 270 Cys Lys Phe Thr Gly Asp Tyr Ser Leu Gln Glu Arg Val Asp Val Gln 275 280 285 Thr Gly Ile Lys Leu Ile Leu Ser Leu Cys Leu Thr Asp Gly Phe Asp 290 295 300 Met Phe Pro Thr Leu Leu Val Thr Asp Gly Ser Cys Met Ile Asp Arg 305 310 315 320 Arg Met Gly Ile His Gly His Pro Leu Glu Ile Gln Ala Leu Phe Tyr 325 330 335 Ser Ala Leu Arg Cys Ser Arg Glu Met Ile Val Met Asn Asp Gly Ser 340 345 350 Lys His Leu Leu Gln Ala Ile Asn Asn Arg Leu Ser Ala Leu Ser Phe 355 360 365 His Ile Arg Glu Tyr Tyr Trp Val Asp Met Lys Lys Ile Asn Glu Ile 370 375 380 Tyr Arg Tyr Lys Thr Glu Glu Tyr Ser His Asp Ala Thr Asn Lys Phe 385 390 395 400 Asn Ile Tyr Pro Glu Gln Ile Pro Ser Trp Leu Val Asp Trp Val Pro 405 410 415 Glu Lys Gly Gly Tyr Leu Ile Gly Asn Leu Gln Pro Ala His Met Asp 420 425 430 Phe Arg Phe Phe Ser Leu Gly Asn Leu Trp Ala Ile Ser Ser Ser Leu 435 440 445 Thr Thr Pro Thr Gln Ala Glu Gly Ile Leu Ser Leu Ile Glu Glu Lys 450 455 460 Trp Asp Asp Leu Val Ala Asn Met Pro Leu Lys Ile Cys Tyr Pro Ala 465 470 475 480 Met Glu Asp Asp Glu Trp Arg Ile Val Thr Gly Ser Asp Pro Lys Asn 485 490 495 Thr Pro Trp Ser Tyr His Asn Gly Gly Ser Trp Pro Thr Leu Leu Trp 500 505 510 Gln Phe Thr Leu Ala Cys Ile Lys Met Gly Arg Pro Glu Leu Ala Arg 515 520 525 Arg Ala Ile Ala Val Ala Glu Glu Lys Leu Ser Ala Asp Lys Trp Pro 530 535 540 Glu Tyr Tyr Asp Thr Arg Ser Gly Arg Phe Val Gly Lys Gln Ser Arg 545 550 555 560 Ser Tyr Gln Thr Trp Thr Ile Ala Gly Phe Leu Thr Ser Lys Ile Leu 565 570 575 Leu Glu Asn Pro Glu Leu Ala Ser Ile Leu Thr Cys Asp Glu Asp Leu 580 585 590 Glu Leu Leu Glu Gly Cys Ala Cys Cys Leu Ser Lys Arg Thr Arg Cys 595 600 605 Ser Arg Arg Val Thr Lys Ser Asp Ile Ile Gly 610 615 55 578 PRT Lolium perenne 55 Met Ala Ile Ala Ala Ala Ala Ala Leu Leu Pro Leu His Leu Gly Cys 1 5 10 15 Ser Asp Ala Ala Pro Arg Arg Pro Gly Asn Ser Leu Arg Ala His Leu 20 25 30 Arg Lys Gly Gly Ile Arg Gly Arg Arg Arg Ser Pro Pro Cys Ala Val 35 40 45 Asn Ser Leu His Pro Ser Gly Asn Pro Lys Thr Pro Gly Gly Gly Asp 50 55 60 Val Gly Gly Gly Arg Gly Val Asn Gly Gly Ala Thr Ala Lys Pro Asp 65 70 75 80 His Ala Pro Pro Ser Gln Arg Arg Arg Ala Pro Arg Asp Val Glu Glu 85 90 95 Glu Ala Trp Ala Leu Leu Arg Glu Ser Val Val Ser Tyr Cys Gly Ser 100 105 110 Pro Val Gly Thr Ile Ala Ala Cys Asp Pro Asn Asp Ala Ser Pro Leu 115 120 125 Asn Tyr Asp Gln Val Phe Ile Arg Asp Phe Val Pro Ser Gly Val Ala 130 135 140 Phe Leu Leu Lys Gly Glu His Glu Ile Val Arg Asn Phe Ile Leu His 145 150 155 160 Thr Leu Gln Leu Gln Ser Trp Glu Lys Thr Ile Asp Cys His Ser Pro 165 170 175 Gly Gln Gly Leu Met Pro Ala Ser Phe Lys Val Arg Val Val Pro Leu 180 185 190 Asp Gly Gly Asp Asp Gly Ala Thr Glu Glu Val Leu Asp Pro Asp Phe 195 200 205 Gly Glu Ala Ala Ile Gly Arg Val Ala Pro Val Asp Ser Gly Leu Trp 210 215 220 Trp Ile Ile Leu Leu Arg Ala Tyr Gly Lys Cys Ser Gly Asp Leu Ser 225 230 235 240 Phe His Glu Arg Val Asp Val Gln Thr Gly Ile Lys Leu Ile Leu Lys 245 250 255 Leu Cys Leu Ala Asp Gly Phe Asp Met Phe Pro Thr Leu Leu Val Thr 260 265 270 Asp Gly Ser Cys Met Met Asp Arg Arg Met Gly Ile His Gly His Pro 275 280 285 Leu Glu Ile Gln Ala Leu Phe Tyr Ser Ala Leu Leu Ser Ala Arg Glu 290 295 300 Met Leu Thr Pro Glu Asp Gly Ser Ala Asp Leu Ile Arg Ala Leu Asn 305 310 315 320 Ser Arg Leu Met Ala Leu Ser Phe His Ile Arg Glu Tyr Tyr Trp Leu 325 330 335 Glu Lys Arg Lys Leu Asn Glu Ile Tyr Arg Tyr Lys Thr Glu Glu Tyr 340 345 350 Ser Tyr Asp Ala Val Asn Lys Phe Asn Ile Tyr Pro Asp Gln Ile Pro 355 360 365 Pro Trp Leu Val Glu Trp Ile Pro Pro Lys Gly Gly Tyr Phe Ile Gly 370 375 380 Asn Leu Gln Pro Ala His Met Asp Phe Arg Phe Phe Ser Leu Gly Asn 385 390 395 400 Leu Trp Ser Ile Val Ser Ser Leu Ala Thr Ala Asp Gln Ser His Ala 405 410 415 Ile Leu Asp Leu Val Glu Ala Lys Trp Ser Asp Leu Val Ala Glu Met 420 425 430 Pro Met Lys Ile Cys Tyr Pro Ala Leu Glu Asp Gln Glu Trp Lys Phe 435 440 445 Ile Thr Gly Ser Asp Pro Lys Asn Thr Pro Trp Ser Tyr His Asn Gly 450 455 460 Gly Ser Trp Pro Thr Leu Leu Trp Gln Leu Thr Val Ala Cys Ile Lys 465 470 475 480 Met Asn Arg Pro Glu Ile Ala Ala Arg Ala Val Glu Val Ala Glu Ser 485 490 495 Arg Ile Ser Thr Asp Lys Trp Pro Glu Tyr Tyr Asp Thr Lys Arg Gly 500 505 510 Arg Phe Ile Gly Lys Gln Ala Arg Leu Phe Gln Thr Trp Ser Ile Ala 515 520 525 Gly Phe Leu Val Ala Lys Leu Leu Leu Glu Asn Pro Glu Lys Ser Arg 530 535 540 Ile Leu Trp Asn Asn Glu Asp Glu Glu Ile Leu Asn Ala Leu Ser Leu 545 550 555 560 Met Thr Gly Pro Ser Ser Pro Lys Arg Lys Arg Gly Arg Lys Thr Tyr 565 570 575 Ile Val 56 554 PRT Lolium perenne 56 Met Lys Arg Val Ser Ser His Val Ser Ile Ala Ser Glu Ala Glu Ile 1 5 10 15 Asn Leu Asp Leu Ser Arg Leu Leu Ile Asp Lys Pro Arg Tyr Thr Leu 20 25 30 Glu Arg Lys Arg Ser Phe Asp Glu Gln Ser Trp Ser Glu Leu Thr His 35 40 45 Thr His Arg Gln Asn Asp Gly Phe Asp Ser Val Leu Gln Ser Pro Ala 50 55 60 Phe Arg Thr Gly Phe Asp Ser Pro Phe Ser Met Gly Thr His Phe Gly 65 70 75 80 Glu Pro Ser Gly Pro His Pro Leu Val Asn Glu Ala Trp Glu Ala Leu 85 90 95 Arg Lys Ser Val Val Tyr Phe Arg Gly Gln Pro Val Gly Thr Ile Ala 100 105 110 Ala Val Asp His Ala Ser Glu Glu Val Leu Asn Tyr Asp Gln Val Phe 115 120 125 Val Arg Asp Phe Val Pro Ser Ala Leu Ala Phe Leu Met Asn Asn Glu 130 135 140 Pro Glu Ile Val Lys Asn Phe Leu Leu Lys Thr Leu His Leu Gln Ser 145 150 155 160 Ser Glu Lys Met Val Asp Arg Phe Lys Leu Gly Ala Gly Ala Met Pro 165 170 175 Ala Ser Phe Lys Val Asp Arg Asn Lys Ser Arg Asn Thr Glu Thr Leu 180 185 190 Val Ala Asp Phe Gly Glu Ser Ala Ile Gly Arg Val Ala Pro Val Asp 195 200 205 Ser Gly Phe Trp Trp Ile Ile Leu Leu Arg Ala Tyr Thr Lys Tyr Thr 210 215 220 Gly Asp Ala Ser Leu Ser Glu Ser Pro Asp Cys Gln Lys Cys Met Arg 225 230 235 240 Leu Ile Leu Asn Leu Cys Leu Ser Glu Gly Phe Asp Thr Phe Pro Thr 245 250 255 Leu Leu Cys Thr Asp Gly Cys Ser Met Ile Asp Arg Arg Met Gly Ile 260 265 270 Tyr Gly Tyr Pro Ile Glu Ile Gln Ala Leu Phe Tyr Met Ala Leu Arg 275 280 285 Cys Ala Leu Gln Met Leu Lys Pro Asp Gly Glu Gly Lys Asp Phe Ile 290 295 300 Glu Lys Ile Gly Gln Arg Leu His Ala Leu Thr Tyr His Met Arg Asn 305 310 315 320 Tyr Phe Trp Leu Asp Phe Pro His Leu Asn Asn Ile Tyr Arg Tyr Lys 325 330 335 Thr Glu Glu Tyr Ser His Thr Ala Val Asn Lys Phe Asn Val Ile Pro 340 345 350 Asp Ser Ile Pro Asp Trp Val Phe Asp Phe Met Pro Cys Arg Gly Gly 355 360 365 Tyr Phe Leu Gly Asn Val Ser Pro Ala Met Met Asp Phe Arg Trp Phe 370 375 380 Ala Leu Gly Asn Cys Ile Ala Ile Ile Ser Ser Leu Ala Thr Pro Glu 385 390 395 400 Gln Ser Ser Ala Ile Met Asp Leu Ile Glu Glu Arg Trp Asp Glu Leu 405 410 415 Val Gly Glu Val Pro Leu Lys Ile Cys Tyr Pro Ala Ile Glu Asn His 420 425 430 Glu Trp Arg Ile Ile Thr Gly Cys Asp Pro Lys Asn Thr Arg Trp Ser 435 440 445 Tyr His Asn Gly Gly Ser Trp Pro Val Leu Leu Trp Leu Leu Thr Ala 450 455 460 Ala Cys Ile Lys Thr Gly Arg Pro Gln Met Ala Lys Arg Ala Ile Glu 465 470 475 480 Leu Ser Glu Ala Arg Leu Leu Lys Asp Gly Trp Pro Glu Tyr Tyr Asp 485 490 495 Gly Lys Leu Gly Lys Phe Val Gly Lys Gln Ala Arg Lys Phe Gln Thr 500 505 510 Trp Ser Ile Ala Gly Tyr Leu Val Ala Arg Met Met Leu Glu Asp Pro 515 520 525 Ser Thr Leu Met Met Ile Ser Met Glu Glu Asp Arg Pro Val Lys Pro 530 535 540 Thr Met Arg Arg Ser Ala Ser Trp Asn Ala 545 550 57 552 PRT Lolium perenne 57 Met Glu Ala Pro Gly Gly Gly Ala Gly Pro Met Pro Thr Thr Pro Ser 1 5 10 15 His Ala Ser Ile Ala Asp Ser Asp Asp Phe Asp Leu Ser Arg Leu Leu 20 25 30 Asn His Arg Pro Arg Ile Asn Val Glu Arg Gln Arg Ser Phe Asp Asp 35 40 45 Arg Ser Leu Gly Asp Leu Tyr Leu Ser Ala Met Asp Ser Arg Gly Gly 50 55 60 Tyr Met Asp Ser Tyr Asp Ser Met Tyr Ser Pro Gly Gly Gly Leu Arg 65 70 75 80 Ser Leu Thr Gly Thr Pro Ala Ser Ser Thr Arg Leu Ser Phe Glu Pro 85 90 95 Gln Leu Leu Val Ala Glu Ala Trp Glu Ala Leu Arg Arg Ser Leu Val 100 105 110 Cys Phe Arg Gly Glu Pro Leu Gly Thr Ile Ala Ala Val Asp Ser Ser 115 120 125 Ser Asp Glu Val Leu Asn Tyr Asp Gln Val Phe Val Arg Asp Phe Val 130 135 140 Pro Ser Ala Leu Ala Phe Leu Met Asn Gly Glu Pro Asp Ile Val Lys 145 150 155 160 Asn Phe Leu Leu Lys Thr Leu Leu Leu Gln Gly Trp Glu Lys Arg Ile 165 170 175 Asp Arg Phe Lys Leu Gly Glu Gly Ala Met Pro Ala Ser Phe Lys Val 180 185 190 Leu Lys Asp Pro Lys Arg Gly Val Asp Thr Leu Ala Ala Asp Phe Gly 195 200 205 Glu Ser Ala Ile Gly Arg Val Ala Pro Ala Asp Ser Gly Phe Trp Trp 210 215 220 Ile Ile Leu Leu Arg Ala Tyr Thr Lys Ser Thr Gly Asp Leu Thr Leu 225 230 235 240 Ala Glu Thr Pro Glu Cys Gln Lys Gly Ile Arg Leu Ile Met Asn Gln 245 250 255 Cys Leu Ala Glu Gly Phe Asp Thr Phe Pro Thr Leu Leu Cys Ala Asp 260 265 270 Gly Cys Cys Met Ile Asp Arg Arg Met Gly Val Tyr Gly Tyr Pro Ile 275 280 285 Glu Ile Gln Ala Leu Phe Phe Met Ser Leu Arg Cys Ala Leu Leu Leu 290 295 300 Leu Lys Pro Ala Val Glu Gly Asn Ser Ser Ser Lys Asp Asp Asp Ile 305 310 315 320 Met Glu Arg Ile Val Thr Arg Leu His Ala Leu Ser Tyr His Met Arg 325 330 335 Ser Tyr Phe Trp Leu Asp Phe Gln Gln Leu Asn Val Ile Tyr Arg Phe 340 345 350 Lys Thr Glu Glu Tyr Ser His Thr Ala Val Asn Lys Phe Asn Val Ile 355 360 365 Pro Glu Ser Ile Pro Asp Trp Leu Phe Asp Phe Met Pro Ser Arg Gly 370 375 380 Gly Tyr Phe Val Gly Asn Val Ser Pro Ala Arg Met Asp Phe Arg Trp 385 390 395 400 Phe Ala Leu Gly Asn Cys Val Ala Ile Leu Ala Ser Leu Ala Thr Pro 405 410 415 Glu Gln Ala Gly Ala Ile Met Asp Leu Ile Glu Glu Arg Trp Glu Asp 420 425 430 Leu Ile Gly Glu Met Pro Leu Lys Ile Cys Tyr Pro Thr Ile Glu Gly 435 440 445 His Glu Trp Gln Asn Val Thr Gly Cys Asp Pro Lys Asn Thr Arg Trp 450 455 460 Ser Tyr His Asn Gly Gly Ser Trp Pro Val Leu Ile Trp Leu Leu Thr 465 470 475 480 Ala Ala Cys Ile Lys Thr Gly Arg Leu Lys Ile Ala Arg Arg Ala Ile 485 490 495 Asp Leu Ala Glu Ala Arg Leu Gly Lys Asp Gly Trp Pro Glu Tyr Tyr 500 505 510 Asp Gly Lys Leu Gly Arg Tyr Val Gly Lys Gln Ala Arg Lys His Gln 515 520 525 Thr Trp Ser Ile Ala Gly Tyr Leu Val Ala Lys Met Met Leu Glu Asp 530 535 540 Pro Ser His Leu Gly Met Ile Ser 545 550 58 562 PRT Lolium perenne 58 Met Glu Phe Gly Ala Pro Gly Gly Met Arg Arg Ser Ala Ser His Asn 1 5 10 15 Ser Leu Ser Gly Ser Asp Asp Phe Asp Leu Thr His Leu Leu Asn Lys 20 25 30 Pro Arg Ile Asn Val Glu Arg Gln Arg Ser Phe Asp Asp Arg Ser Leu 35 40 45 Ser Asp Val Ser Tyr Ser Gly Gly Gly His Ala Arg Gly Ala Gly Gly 50 55 60 Gly Phe Asp Gly Met Tyr Ser Pro Gly Gly Gly Leu Arg Ser Leu Val 65 70 75 80 Gly Thr Pro Ala Ser Ser Ala Leu His Ser Phe Glu Pro His Pro Ile 85 90 95 Val Gly Asp Ala Trp Glu Ala Leu Arg Arg Ser Leu Val Phe Phe Arg 100 105 110 Gly Gln Pro Leu Gly Thr Ile Ala Ala Tyr Asp His Ala Ser Glu Glu 115 120 125 Val Leu Asn Tyr Asp Gln Val Phe Val Arg Asp Phe Val Pro Ser Ala 130 135 140 Met Ala Phe Leu Met Asn Gly Glu Pro Glu Ile Val Lys Asn Phe Leu 145 150 155 160 Leu Lys Thr Val Leu Leu Gln Gly Trp Glu Lys Lys Val Asp Arg Phe 165 170 175 Lys Leu Gly Glu Gly Ala Met Pro Ala Ser Phe Lys Val Leu His Asp 180 185 190 Asp Lys Lys Gly Val Asp Thr Leu His Ala Asp Phe Gly Glu Ser Ala 195 200 205 Ile Gly Arg Val Ala Pro Val Asp Ser Gly Phe Trp Trp Ile Ile Leu 210 215 220 Leu Arg Ala Tyr Thr Lys Ser Thr Gly Asp Leu Thr Leu Ala Glu Lys 225 230 235 240 Pro Glu Cys Gln Lys Ala Met Arg Leu Ile Leu Ser Leu Cys Leu Ser 245 250 255 Glu Gly Phe Asp Thr Phe Pro Thr Leu Leu Cys Ala Asp Gly Cys Cys 260 265 270 Met Ile Asp Arg Arg Met Gly Val Tyr Gly Tyr Pro Ile Glu Ile Gln 275 280 285 Ser Leu Phe Phe Met Ala Leu Arg Cys Ala Leu Leu Met Leu Lys His 290 295 300 Asp Asn Glu Gly Lys Asp Phe Val Glu Arg Ile Ala Thr Arg Leu His 305 310 315 320 Ala Leu Ser Tyr His Met Arg Ser Tyr Phe Trp Leu Asp Phe Gln Gln 325 330 335 Leu Asn Asp Ile Tyr Arg Tyr Lys Thr Glu Glu Tyr Ser His Thr Ala 340 345 350 Val Asn Lys Phe Asn Val Ile Pro Asp Ser Ile Pro Asp Trp Leu Phe 355 360 365 Asp Phe Met Pro Cys Glu Gly Gly Phe Phe Val Gly Asn Val Ser Pro 370 375 380 Ala Arg Met Asp Phe Arg Trp Phe Ala Leu Gly Asn Met Ile Ala Ile 385 390 395 400 Val Ser Ser Leu Ala Thr Pro Glu Gln Ser Thr Ala Ile Met Asp Leu 405 410 415 Ile Glu Glu Arg Trp Glu Glu Leu Ile Gly Glu Met Pro Leu Lys Ile 420 425 430 Cys Tyr Pro Ala Ile Glu Asn His Glu Trp Arg Ile Val Thr Gly Cys 435 440 445 Asp Pro Lys Asn Thr Arg Trp Ser Tyr His Asn Gly Gly Ser Trp Pro 450 455 460 Val Leu Leu Trp Leu Leu Thr Ala Ala Ser Ile Lys Thr Gly Arg Pro 465 470 475 480 Gln Ile Ala Arg Arg Ala Ile Asp Leu Ala Glu Arg Arg Leu Leu Lys 485 490 495 Asp Gly Trp Pro Glu Tyr Tyr Asp Gly Lys Leu Gly Lys Tyr Val Gly 500 505 510 Lys Gln Ala Arg Lys Phe Gln Thr Trp Ser Ile Ala Gly Tyr Leu Val 515 520 525 Ala Lys Met Leu Leu Glu Asp Pro Ser His Leu Gly Met Ile Ala Leu 530 535 540 Glu Glu Asp Lys Ala Met Lys Pro Val Leu Arg Arg Ser Ala Ser Trp 545 550 555 560 Thr Asn 59 616 PRT Lolium perenne 59 Met Asp Ser Asp Tyr Gly Val Pro Arg Glu Leu Ser Glu Val Gln Lys 1 5 10 15 Lys Arg Thr Leu Tyr Gln Pro Asp Leu Pro Pro Cys Leu Gln Gly Thr 20 25 30 Thr Val Arg Val Glu Tyr Gly Asp Val Ala Ile Ala Ala Asp Pro Ala 35 40 45 Gly Ala His Val Ile Ser His Ala Phe Pro His Thr Tyr Gly Gln Pro 50 55 60 Leu Ala His Phe Leu Arg Lys Ala Ala Asn Val Ala Asp Ala Lys Val 65 70 75 80 Ile Ser Glu His Pro Ala Val Arg Val Gly Ile Val Phe Cys Gly Arg 85 90 95 Gln Ser Pro Gly Gly His Asn Val Ile Trp Gly Leu His Asp Ala Ile 100 105 110 Lys Ala His Asn Pro Asn Ser Lys Leu Ile Gly Phe Leu Gly Gly Ser 115 120 125 Asp Gly Leu Leu Ala Gln Lys Thr Leu Glu Ile Thr Asp Glu Val Leu 130 135 140 Ser Ser Tyr Lys Asn Gln Gly Gly Tyr Asp Met Leu Gly Arg Thr Lys 145 150 155 160 Asp Gln Ile Arg Thr Thr Glu Gln Val Asn Gly Ala Met Ala Ser Cys 165 170 175 Gln Ala Leu Lys Leu Asp Ala Leu Ile Ile Ile Gly Gly Val Thr Ser 180 185 190 Asn Thr Asp Ala Ala Gln Leu Ala Glu Thr Phe Ala Glu Ala Lys Cys 195 200 205 Ala Thr Lys Val Val Gly Val Pro Val Thr Leu Asn Gly Asp Leu Lys 210 215 220 Asn Gln Phe Val Glu Thr Thr Val Gly Phe Asp Thr Ile Cys Lys Val 225 230 235 240 Asn Ser Gln Leu Ile Ser Asn Met Cys Thr Asp Ala Leu Ser Ala Glu 245 250 255 Lys Tyr Tyr Tyr Phe Ile Arg Met Met Gly Arg Lys Ala Ser His Val 260 265 270 Ala Leu Glu Cys Ala Leu Gln Ser His Pro Asn Met Val Ile Leu Gly 275 280 285 Glu Glu Val Ala Ala Ser Lys Leu Thr Ile Phe Asp Ile Thr Lys Gln 290 295 300 Ile Cys Asp Ala Val Gln Ala Arg Ala Glu Lys Asp Lys Asn His Gly 305 310 315 320 Val Ile Leu Ile Pro Glu Gly Leu Val Glu Ser Ile Pro Glu Leu Tyr 325 330 335 Ala Leu Leu Gln Glu Ile Asn Gly Leu His Gly Lys Gly Val Ser Ile 340 345 350 Glu Asn Ile Ser Ser Gln Leu Ser Pro Trp Ala Ser Ala Leu Phe Glu 355 360 365 Phe Leu Pro Gln Phe Ile Arg Gln Gln Leu Leu Leu Arg Pro Glu Ser 370 375 380 Asp Asp Ser Ala Gln Leu Ser Gln Ile Glu Thr Glu Lys Leu Leu Ala 385 390 395 400 Gln Leu Val Glu Thr Glu Met Asn Lys Arg Leu Lys Glu Gly Thr Tyr 405 410 415 Lys Gly Lys Lys Phe Asn Ala Ile Cys His Phe Phe Gly Tyr Gln Ala 420 425 430 Arg Gly Ala Met Pro Ser Lys Phe Asp Cys Asp Tyr Ala Tyr Val Leu 435 440 445 Gly His Val Ser Tyr His Ile Leu Ala Ala Gly Leu Asn Gly Tyr Met 450 455 460 Ala Thr Val Thr Asn Leu Lys Ser Pro Leu Asn Lys Trp Arg Cys Gly 465 470 475 480 Ala Ala Pro Ile Ser Ser Met Met Thr Val Lys Arg Trp Ser Arg Gly 485 490 495 Pro Ser Thr Thr Gln Ile Gly Lys Pro Ala Val His Met Ala Ser Val 500 505 510 Asp Leu Arg Gly Lys Ala Tyr Glu Leu Leu Arg Gln Asn Ser Ser Ser 515 520 525 Cys Leu Leu Glu Asp Ile Tyr Arg Asn Pro Gly Pro Leu Gln Phe Glu 530 535 540 Gly Pro Gly Ser Asp Ser Lys Pro Ile Ser Leu Cys Val Glu Asp Gln 545 550 555 560 Asp Tyr Met Gly Arg Ile Lys Lys Leu Gln Glu Tyr Leu Glu Lys Val 565 570 575 Lys Ser Ile Val Lys Pro Gly Cys Ser Gln Asp Val Leu Lys Ala Ala 580 585 590 Leu Ser Ala Met Ser Ser Val Thr Asp Thr Leu Ala Ile Met Thr Ser 595 600 605 Ser Ser Thr Gly Gln Ala Pro Leu 610 615 60 616 PRT Festuca arundinacea 60 Met Asp Ser Asp Tyr Gly Val Pro Arg Glu Leu Ser Glu Val Gln Lys 1 5 10 15 Lys Arg Thr Leu Tyr Gln Pro Glu Leu Pro Pro Cys Leu Gln Gly Thr 20 25 30 Thr Val Arg Val Glu Tyr Gly Asp Val Ala Ile Ala Ala Asp Pro Ala 35 40 45 Gly Ala His Val Ile Ser His Ala Phe Pro His Thr Tyr Gly Gln Pro 50 55 60 Leu Ala His Phe Leu Arg Lys Ala Ala Asn Val Ala Asp Ala Lys Val 65 70 75 80 Ile Ser Glu His Pro Ala Val Arg Val Gly Ile Val Phe Cys Gly Arg 85 90 95 Gln Ser Pro Gly Gly His Asn Val Ile Trp Gly Leu His Asp Ala Ile 100 105 110 Lys Ala His Asn Ser Asn Ser Lys Leu Ile Gly Phe Leu Gly Gly Ser 115 120 125 Asp Gly Leu Leu Ala Gln Lys Thr Leu Glu Ile Thr Asp Glu Val Leu 130 135 140 Ser Ser Tyr Lys Asn Gln Gly Gly Tyr Asp Met Leu Gly Arg Thr Lys 145 150 155 160 Asp Gln Ile Arg Thr Thr Glu Gln Val Asn Gly Ala Met Ala Ser Cys 165 170 175 Gln Asp Leu Lys Leu Asp Ala Leu Ile Ile Ile Gly Gly Val Thr Ser 180 185 190 Asn Thr Asp Ala Ala Gln Leu Ala Glu Thr Phe Ala Glu Ala Lys Cys 195 200 205 Ala Thr Lys Val Val Gly Val Pro Val Thr Leu Asn Gly Asp Leu Lys 210 215 220 Asn Gln Phe Val Glu Thr Thr Val Gly Phe Asp Thr Ile Cys Lys Val 225 230 235 240 Asn Ser Gln Leu Ile Ser Asn Met Cys Thr Asp Ala Leu Ser Ala Glu 245 250 255 Lys Tyr Tyr Tyr Phe Ile Arg Met Met Gly Arg Lys Ala Ser His Val 260 265 270 Ala Leu Glu Cys Ala Leu Gln Ser His Pro Asn Met Val Ile Leu Gly 275 280 285 Glu Glu Val Ala Ala Ser Lys Leu Thr Ile Phe Asp Ile Thr Lys Gln 290 295 300 Ile Cys Asp Ala Val Gln Ala Arg Ala Glu Lys Asp Lys Asn His Gly 305 310 315 320 Val Ile Leu Ile Pro Glu Gly Leu Val Glu Ser Ile Pro Glu Leu Tyr 325 330 335 Ala Leu Leu Gln Glu Ile Asn Gly Leu His Gly Lys Gly Val Ser Ile 340 345 350 Glu Asn Ile Ser Ser Gln Leu Ser Pro Trp Ala Ser Ala Leu Phe Glu 355 360 365 Phe Leu Pro Gln Phe Ile Arg His Gln Leu Leu Leu Arg Pro Glu Ser 370 375 380 Asp Asp Ser Ala Gln Leu Ser Gln Ile Glu Thr Glu Lys Leu Leu Ala 385 390 395 400 Gln Leu Val Glu Thr Glu Met Asn Lys Arg Leu Lys Glu Gly Thr Tyr 405 410 415 Lys Gly Lys Lys Phe Asn Ala Ile Cys His Phe Phe Gly Tyr Gln Ala 420 425 430 Arg Gly Ala Met Pro Ser Lys Phe Asp Cys Asp Tyr Ala Tyr Val Leu 435 440 445 Gly His Val Ser Tyr His Ile Leu Ala Ala Gly Leu Asn Gly Tyr Met 450 455 460 Ala Thr Val Thr Asn Leu Lys Ser Pro Leu Asn Lys Trp Arg Cys Gly 465 470 475 480 Ala Ala Pro Ile Ser Ser Met Met Thr Val Lys Arg Trp Ser Arg Gly 485 490 495 Pro Ser Thr Thr Gln Ile Gly Lys Pro Ala Met His Met Ala Thr Val 500 505 510 Asp Leu Arg Gly Lys Ala Tyr Glu Leu Leu Arg Gln Asn Ser Ser Ser 515 520 525 Tyr Leu Leu Glu Asp Ile Tyr Arg Asn Pro Gly Pro Leu Gln Phe Glu 530 535 540 Gly Pro Gly Ala Asp Ser Lys Pro Ile Ser Leu Cys Val Glu Asp Gln 545 550 555 560 Asp Tyr Met Gly Arg Ile Lys Lys Leu Gln Glu Tyr Leu Glu Lys Val 565 570 575 Lys Ser Ile Val Lys Pro Gly Cys Ser Gln Asp Val Leu Lys Ala Ala 580 585 590 Leu Ser Ala Met Ser Ser Val Thr Glu Thr Leu Ala Ile Met Thr Ser 595 600 605 Ser Ser Thr Gly Gln Ala Pro Leu 610 615 61 563 PRT Lolium perenne 61 Met Ala Ala Ala Ala Val Ala Thr Ser Asn Gly Ala Ser Ala Asn Gly 1 5 10 15 Pro Thr Pro Gly Arg Leu Ala Ser Val Tyr Ser Glu Val Gln Thr Ser 20 25 30 Arg Ile Ala His Ala Leu Pro Leu Pro Ser Val Leu Arg Ser His Phe 35 40 45 Thr Leu Ala Asp Gly Ala Ala Ser Ser Ala Thr Gly Asn Pro Glu Glu 50 55 60 Ile Ala Lys Leu Phe Pro Asn Leu Tyr Gly Gln Pro Ser Ala Ala Val 65 70 75 80 Val Pro Ser Ala Gln Pro Val Ala Thr Lys Pro Leu Lys Ile Gly Val 85 90 95 Val Leu Ser Gly Gly Gln Ala Pro Gly Gly His Asn Val Ile Cys Gly 100 105 110 Ile Phe Asp Tyr Leu Gln Glu Arg Ala Lys Gly Ser Thr Met Tyr Gly 115 120 125 Phe Lys Gly Gly Pro Ala Gly Val Met Lys Gly Lys Tyr Val Glu Leu 130 135 140 Asn Ala Asp Phe Val Tyr Pro Tyr Arg Asn Gln Gly Gly Phe Asp Met 145 150 155 160 Ile Cys Ser Gly Arg Asp Lys Ile Glu Thr Pro Glu Gln Phe Lys Gln 165 170 175 Ala Glu Asp Thr Val Thr Lys Leu Asp Leu Asp Gly Leu Val Val Ile 180 185 190 Gly Gly Asp Asp Ser Asn Thr Asn Ala Cys Leu Leu Gly Glu Tyr Phe 195 200 205 Arg Gly Arg Asn Leu Lys Thr Arg Val Ile Gly Cys Pro Lys Thr Ile 210 215 220 Asp Gly Asp Leu Lys Cys Lys Glu Val Pro Thr Ser Phe Gly Phe Asp 225 230 235 240 Thr Ala Cys Lys Ile Tyr Ser Glu Met Ile Gly Asn Val Met Thr Asp 245 250 255 Ala Arg Ser Thr Gly Lys Tyr Tyr His Phe Val Arg Leu Met Gly Arg 260 265 270 Ala Ala Ser His Ile Thr Leu Glu Cys Ala Leu Gln Thr His Pro Asn 275 280 285 Val Ser Leu Ile Gly Glu Glu Val Ala Glu Lys Lys Glu Thr Leu Lys 290 295 300 Gln Val Thr Asp Tyr Ile Thr Asp Val Ile Cys Lys Arg Ala Glu Leu 305 310 315 320 Gly Tyr Asn Tyr Gly Val Ile Leu Ile Pro Glu Gly Leu Ile Asp Phe 325 330 335 Ile Pro Glu Val Gln Lys Leu Ile Ala Glu Leu Asn Glu Ile Leu Ala 340 345 350 His Asp Val Val Asp Glu Ala Gly Ala Trp Lys Ser Lys Leu Gln Pro 355 360 365 Glu Ser Arg Gln Leu Phe Asp Phe Leu Pro Asn Thr Ile Gln Glu Gln 370 375 380 Leu Leu Leu Glu Arg Asp Pro His Gly Asn Val Gln Val Ala Lys Ile 385 390 395 400 Glu Thr Glu Lys Met Leu Ile Ala Met Val Glu Thr Glu Leu Glu Lys 405 410 415 Arg Arg Ser Ala Gly Lys Tyr Ser Ala His Phe Arg Gly Gln Ser His 420 425 430 Phe Phe Gly Tyr Glu Gly Arg Cys Gly Leu Pro Thr Asn Phe Asp Ser 435 440 445 Ser Tyr Cys Tyr Ala Leu Gly Tyr Gly Ala Gly Ala Leu Leu Gln Phe 450 455 460 Gly Lys Thr Gly Leu Ile Ser Ser Val Gly Asn Leu Ala Ala Pro Val 465 470 475 480 Glu Glu Trp Thr Val Gly Gly Thr Pro Leu Thr Ala Leu Met Asp Val 485 490 495 Glu Arg Arg His Gly Lys Phe Lys Pro Val Ile Lys Lys Ala Met Val 500 505 510 Glu Leu Asp Ala Ala Pro Phe Lys Lys Phe Ala Ser Met Arg Asp Glu 515 520 525 Trp Ala Ile Lys Asn Arg Tyr Ile Ser Pro Gly Pro Ile Gln Phe Ser 530 535 540 Gly Pro Gly Ser Asp Ala Ser Asn His Thr Leu Met Leu Glu Leu Gly 545 550 555 560 Ala Gln Thr 62 563 PRT Lolium perenne 62 Met Ala Ala Ala Ala Val Ala Thr Ser Asn Gly Ala Ser Ala Asn Gly 1 5 10 15 Pro Thr Pro Gly Arg Leu Ala Ser Val Tyr Ser Glu Val Gln Thr Ser 20 25 30 Arg Ile Ala His Ala Leu Pro Leu Pro Ser Val Leu Arg Ser His Phe 35 40 45 Thr Leu Ala Asp Gly Ala Ala Ser Ser Ala Thr Gly Asn Pro Glu Glu 50 55 60 Ile Ala Lys Leu Phe Pro Asn Leu Tyr Gly Gln Pro Ser Ala Ala Val 65 70 75 80 Val Pro Ser Ala Gln Pro Val Ala Thr Lys Pro Leu Lys Ile Gly Val 85 90 95 Val Leu Ser Gly Gly Gln Ala Pro Gly Gly His Asn Val Ile Cys Gly 100 105 110 Ile Phe Asp Tyr Leu Gln Glu Arg Ala Lys Gly Ser Thr Met Tyr Gly 115 120 125 Phe Lys Gly Gly Pro Ala Gly Val Met Lys Gly Lys Tyr Val Glu Leu 130 135 140 Asn Ala Asp Phe Val Tyr Pro Tyr Arg Asn Gln Gly Gly Phe Asp Met 145 150 155 160 Ile Cys Ser Gly Arg Asp Lys Ile Glu Thr Pro Glu Gln Phe Lys Gln 165 170 175 Ala Glu Asp Thr Val Thr Lys Leu Asp Leu Asp Gly Leu Val Val Ile 180 185 190 Gly Gly Asp Asp Ser Asn Thr Asn Ala Cys Leu Leu Gly Glu Tyr Phe 195 200 205 Arg Gly Arg Asn Leu Lys Thr Arg Val Ile Gly Cys Pro Lys Thr Ile 210 215 220 Asp Gly Asp Leu Lys Cys Lys Glu Val Pro Thr Ser Phe Gly Phe Asp 225 230 235 240 Thr Ala Cys Lys Ile Tyr Ser Glu Met Ile Gly Asn Val Met Thr Asp 245 250 255 Ala Arg Ser Thr Gly Lys Tyr Tyr His Phe Val Arg Leu Met Gly Arg 260 265 270 Ala Ala Ser His Ile Thr Leu Glu Cys Ala Leu Gln Thr His Pro Asn 275 280 285 Val Ser Leu Ile Gly Glu Glu Val Ala Glu Lys Lys Glu Thr Leu Lys 290 295 300 Gln Val Thr Asp Tyr Ile Thr Asp Val Ile Cys Lys Arg Ala Glu Leu 305 310 315 320 Gly Tyr Asn Tyr Gly Val Ile Leu Ile Pro Glu Gly Leu Ile Asp Phe 325 330 335 Ile Pro Glu Val Gln Lys Leu Ile Ala Glu Leu Asn Glu Ile Leu Ala 340 345 350 His Asp Val Val Asp Glu Ala Gly Ala Trp Lys Ser Lys Leu Gln Pro 355 360 365 Glu Ser Arg Gln Leu Phe Asp Phe Leu Pro Asn Thr Ile Gln Glu Gln 370 375 380 Leu Leu Leu Glu Arg Asp Pro His Gly Asn Val Gln Val Ala Lys Ile 385 390 395 400 Glu Thr Glu Lys Met Leu Ile Ala Met Val Glu Thr Glu Leu Glu Lys 405 410 415 Arg Arg Ser Ala Gly Lys Tyr Ser Ala His Phe Arg Gly Gln Ser His 420 425 430 Phe Phe Gly Tyr Glu Gly Arg Cys Gly Leu Pro Thr Asn Phe Asp Ser 435 440 445 Ser Tyr Cys Tyr Ala Leu Gly Tyr Gly Ala Gly Ala Leu Leu Gln Phe 450 455 460 Gly Lys Thr Gly Leu Ile Ser Ser Val Gly Asn Leu Ala Ala Pro Val 465 470 475 480 Glu Glu Trp Thr Val Gly Gly Thr Pro Leu Thr Ala Leu Met Asp Val 485 490 495 Glu Arg Arg His Gly Lys Phe Lys Pro Val Ile Lys Lys Ala Met Val 500 505 510 Glu Leu Asp Ala Ala Pro Phe Lys Lys Phe Ala Ser Met Arg Asp Glu 515 520 525 Trp Ala Ile Lys Asn Arg Tyr Ile Ser Pro Gly Pro Ile Gln Phe Ser 530 535 540 Gly Pro Gly Ser Asp Ala Ser Asn His Thr Leu Met Leu Glu Leu Gly 545 550 555 560 Ala Gln Thr 63 563 PRT Festuca arundinacea 63 Met Ala Ala Ala Ala Val Ala Thr Ser Asn Gly Ala Ser Ala Asn Gly 1 5 10 15 Pro Thr Pro Gly Arg Leu Ala Ser Val Tyr Ser Glu Val Gln Thr Ser 20 25 30 Arg Ile Ala His Ala Leu Pro Leu Pro Ser Val Leu Arg Ser Asn Phe 35 40 45 Thr Leu Ala Asp Gly Pro Ala Ser Ser Ala Thr Gly Asn Pro Glu Glu 50 55 60 Ile Ala Lys Leu Phe Pro Asn Leu Tyr Gly Gln Pro Ser Ala Ala Val 65 70 75 80 Val Pro Ser Ala Glu Pro Val Pro Thr Lys Pro Leu Lys Ile Gly Val 85 90 95 Val Leu Ser Gly Gly Gln Ala Pro Gly Gly His Asn Val Ile Cys Gly 100 105 110 Ile Phe Asp Tyr Leu Gln Glu Arg Ala Lys Gly Ser Thr Met Tyr Gly 115 120 125 Phe Lys Gly Gly Pro Ala Gly Ile Met Lys Gly Lys Tyr Ile Glu Leu 130 135 140 Asn Ala Asp Phe Val Tyr Pro Tyr Arg Asn Gln Gly Gly Phe Asp Met 145 150 155 160 Ile Cys Ser Gly Arg Asp Lys Ile Glu Thr Pro Glu Gln Phe Lys Gln 165 170 175 Ala Glu Asp Thr Val Asn Lys Leu Asp Leu Asp Gly Leu Val Val Ile 180 185 190 Gly Gly Asp Asp Ser Asn Thr Asn Ala Cys Leu Leu Gly Glu Tyr Phe 195 200 205 Arg Gly Arg Asn Leu Lys Thr Arg Val Ile Gly Cys Pro Lys Thr Ile 210 215 220 Asp Gly Asp Leu Lys Cys Lys Glu Val Pro Ile Ser Phe Gly Phe Asp 225 230 235 240 Thr Ala Cys Lys Ile Tyr Ser Glu Met Ile Gly Asn Val Met Thr Asp 245 250 255 Ala Arg Ser Thr Gly Lys Tyr Tyr His Phe Val Arg Leu Met Gly Arg 260 265 270 Ala Ala Ser His Ile Thr Leu Glu Cys Ala Leu Gln Thr His Pro Asn 275 280 285 Val Ser Leu Ile Gly Glu Glu Val Ala Glu Lys Lys Glu Thr Leu Lys 290 295 300 Gln Val Thr Asp Tyr Ile Thr Asp Val Ile Cys Lys Arg Ala Glu Leu 305 310 315 320 Gly Tyr Asn Tyr Gly Val Ile Leu Ile Pro Glu Gly Leu Ile Asp Phe 325 330 335 Ile Pro Glu Val Gln Lys Leu Ile Ala Glu Leu Asn Glu Ile Leu Ala 340 345 350 His Asp Val Val Asp Glu Ala Gly Ala Trp Lys Ser Lys Leu Gln Pro 355 360 365 Glu Ser Arg Gln Leu Phe Asp Phe Leu Pro Asn Thr Ile Gln Glu Gln 370 375 380 Leu Leu Leu Glu Arg Asp Pro His Gly Asn Val Gln Val Ala Lys Ile 385 390 395 400 Glu Thr Glu Lys Met Leu Ile Ala Met Val Glu Thr Glu Leu Glu Lys 405 410 415 Arg Arg Ala Ala Gly Lys Tyr Ser Ala His Phe Arg Gly Gln Ser His 420 425 430 Phe Phe Gly Tyr Glu Gly Arg Cys Gly Leu Pro Thr Asn Phe Asp Ser 435 440 445 Ser Tyr Cys Tyr Ala Leu Gly Tyr Gly Ala Gly Ala Leu Leu Gln Phe 450 455 460 Gly Lys Thr Gly Leu Ile Ser Ser Val Gly Asn Leu Ala Ala Pro Val 465 470 475 480 Glu Glu Trp Thr Val Gly Gly Thr Pro Leu Thr Ala Leu Met Asp Val 485 490 495 Glu Arg Arg His Gly Lys Phe Lys Pro Val Ile Lys Lys Ala Met Val 500 505 510 Glu Leu Asp Ala Ala Pro Phe Lys Lys Phe Ala Ser Met Arg Asp Glu 515 520 525 Trp Ala Ile Lys Asn Arg Tyr Ile Ser Pro Gly Pro Ile Gln Phe Ser 530 535 540 Gly Pro Gly Ser Asp Ala Ser Asn His Thr Leu Met Leu Glu Leu Gly 545 550 555 560 Ala Gln Ile 64 964 PRT Lolium perenne 64 Met Val Gly Asn Asp Asn Trp Ile Asn Ser Tyr Leu Asp Ala Ile Leu 1 5 10 15 Asp Ala Gly Lys Ser Ser Ile Gly Gly Asp Arg Pro Ser Leu Leu Leu 20 25 30 Arg Glu Arg Gly His Phe Ser Pro Ala Arg Tyr Phe Val Glu Glu Val 35 40 45 Ile Thr Gly Tyr Asp Glu Thr Asp Leu Tyr Lys Thr Trp Leu Arg Ala 50 55 60 Asn Ala Met Arg Ser Pro Gln Glu Arg Asn Thr Arg Leu Glu Asn Met 65 70 75 80 Thr Trp Arg Ile Trp Asn Leu Ala Arg Lys Lys Lys Glu Leu Glu Lys 85 90 95 Glu Glu Ala Cys Arg Leu Leu Lys Arg His Pro Glu Thr Glu Lys Thr 100 105 110 Arg Thr Asp Ala Thr Ala Asp Met Ser Glu Asp Leu Phe Asp Gly Glu 115 120 125 Lys Gly Glu Asp Ala Gly Asp Pro Ser Val Ala Tyr Gly Asp Ser Thr 130 135 140 Thr Gly Ser Ser Pro Lys Thr Ser Ser Val Asp Lys Leu Tyr Ile Val 145 150 155 160 Leu Ile Ser Leu His Gly Leu Val Arg Gly Glu Asn Met Glu Leu Gly 165 170 175 Arg Asp Ser Asp Thr Gly Gly Gln Val Lys Tyr Val Val Glu Phe Ala 180 185 190 Lys Ala Leu Ser Ser Ser Pro Gly Val Tyr Arg Val Asp Leu Leu Thr 195 200 205 Arg Gln Ile Val Ala Pro Asn Phe Asp Arg Ser Tyr Gly Glu Pro Glu 210 215 220 Glu Met Leu Val Ser Thr Thr Phe Lys Asn Ser Lys His Glu Arg Gly 225 230 235 240 Val Asn Ser Gly Gly Tyr Ile Ile Arg Ile Pro Phe Gly Pro Lys Asp 245 250 255 Lys Tyr Leu Ala Lys Glu His Met Trp Pro Phe Ile Gln Asp Phe Val 260 265 270 Asp Gly Ala Leu Ser His Ile Leu Arg Met Ser Lys Thr Ile Gly Glu 275 280 285 Glu Ile Gly Cys Gly His Pro Val Trp Pro Ala Val Ile His Gly His 290 295 300 Tyr Ala Ser Ala Gly Val Ala Ala Ala Leu Leu Ser Gly Ala Leu Asn 305 310 315 320 Leu Pro Met Ala Phe Thr Gly His Phe Leu Gly Lys Asp Lys Leu Glu 325 330 335 Gly Leu Leu Lys Gln Gly Arg Gln Ser Arg Glu Gln Ile Asn Met Thr 340 345 350 Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu Glu Leu Ser Leu Asp Ala 355 360 365 Ser Glu Ile Val Ile Ala Ser Thr Arg Gln Glu Ile Glu Glu Gln Trp 370 375 380 Asn Leu Tyr Asp Gly Phe Glu Val Ile Leu Ala Arg Lys Leu Arg Ala 385 390 395 400 Arg Val Lys Arg Gly Ala Asn Cys Tyr Gly Arg Tyr Met Pro Arg Met 405 410 415 Val Ile Ile Pro Pro Gly Val Glu Phe Gly His Val Val His Asp Phe 420 425 430 Asp Met Asp Gly Glu Glu Glu Asn His Gly Pro Ala Ser Glu Asp Pro 435 440 445 Pro Ile Trp Ser Gln Ile Met Arg Phe Phe Thr Asn Pro Arg Lys Pro 450 455 460 Met Ile Leu Ala Val Ala Arg Pro Tyr Pro Glu Lys Asn Ile Thr Ser 465 470 475 480 Leu Val Lys Ala Phe Gly Glu Cys Arg Pro Leu Arg Glu Leu Ala Asn 485 490 495 Leu Thr Leu Ile Met Gly Asn Arg Glu Ala Ile Ser Lys Met His Asn 500 505 510 Thr Ser Ala Ser Val Leu Thr Ser Val Leu Thr Leu Ile Asp Glu Tyr 515 520 525 Asp Leu Tyr Gly Gln Val Ala Tyr Pro Lys His His Lys His Ser Glu 530 535 540 Val Pro Asp Ile Tyr Arg Leu Ala Thr Arg Thr Lys Gly Ala Phe Val 545 550 555 560 Asn Val Ala Tyr Phe Glu Gln Phe Gly Val Thr Leu Ile Glu Ala Ala 565 570 575 Met Asn Gly Leu Pro Val Ile Ala Thr Lys Asn Gly Ala Pro Val Glu 580 585 590 Ile Asn Gln Val Leu Asn Asn Gly Leu Leu Val Asp Pro His Asp Gln 595 600 605 Asn Ala Ile Ala Asp Ala Leu Tyr Lys Leu Leu Ser Glu Lys Gln Leu 610 615 620 Trp Ser Arg Cys Arg Glu Asn Gly Leu Lys Asn Ile His Gln Phe Ser 625 630 635 640 Trp Pro Glu His Cys Lys Asn His Leu Ser Arg Ile Leu Thr Leu Gly 645 650 655 Ala Arg Ser Pro Ala Ile Gly Ser Lys Glu Glu Arg Ser Asn Ala Pro 660 665 670 Ile Ser Gly Arg Lys His Ile Ile Val Ile Ser Val Asp Ser Val Asn 675 680 685 Lys Glu Asp Leu Val Arg Ile Ile Arg Asn Ala Ile Glu Ala Ala His 690 695 700 Thr Gln Asn Thr Pro Ala Ser Thr Gly Phe Val Leu Ser Thr Ser Leu 705 710 715 720 Thr Leu Ser Glu Ile Cys Ser Leu Leu Val Ser Val Gly Met His Pro 725 730 735 Ala Gly Phe Asp Ala Phe Ile Cys Asn Ser Gly Ser Ser Ile Tyr Tyr 740 745 750 Pro Ser Tyr Ser Gly Asn Thr Pro Ser Ser Ser Lys Val Thr His Val 755 760 765 Ile Asp Gln Asn His Gln Ser His Ile Glu Tyr Arg Trp Gly Gly Glu 770 775 780 Gly Leu Arg Lys Tyr Leu Val Lys Trp Ala Thr Ser Val Val Glu Arg 785 790 795 800 Lys Gly Arg Ile Glu Arg Gln Met Ile Phe Glu Asp Ser Glu His Ser 805 810 815 Ser Thr Tyr Cys Leu Ala Phe Lys Val Val Asn Pro Asn His Leu Pro 820 825 830 Pro Leu Lys Glu Leu Arg Lys Leu Met Arg Ile Gln Ser Leu Arg Cys 835 840 845 Asn Ala Leu Tyr Asn His Ser Ala Thr Arg Leu Ser Val Thr Pro Ile 850 855 860 His Ala Ser Arg Ser Gln Ala Ile Arg Tyr Leu Phe Ile Arg Trp Gly 865 870 875 880 Ile Glu Leu Pro Asn Ile Val Val Leu Val Gly Glu Ser Gly Asp Ser 885 890 895 Asp Tyr Glu Glu Leu Leu Gly Gly Leu His Arg Thr Ile Ile Leu Lys 900 905 910 Gly Asp Phe Asn Ile Ala Ala Asn Arg Ile His Thr Val Arg Arg Tyr 915 920 925 Pro Leu Gln Asp Val Val Ala Leu Asp Ser Ser Asn Ile Ile Glu Val 930 935 940 Glu Gly Cys Thr Thr Asp Val Ile Lys Ser Ala Leu Arg Gln Ile Gly 945 950 955 960 Val Pro Thr Gln 65 984 PRT Festuca arundinacea VARIANT (1)...(984) Xaa = Any Amino Acid 65 Met Val Gly Gly Met Cys Gly Asn Asp Asn Trp Ile Asn Ser Tyr Leu 1 5 10 15 Asp Ala Ile Leu Asp Ala Gly Lys Gly Ala Pro Gly Gly Gly Ala Gly 20 25 30 Pro Gly Gly Gly Arg Gly Gly Gly Gly Gly Gly Ala Gly Asp Arg Pro 35 40 45 Ser Leu Leu Leu Arg Glu Arg Gly His Phe Ser Pro Ala Arg Tyr Phe 50 55 60 Val Glu Glu Val Ile Thr Gly Tyr Asp Glu Thr Asp Leu Tyr Lys Thr 65 70 75 80 Trp Ser Arg Ala Asn Ala Met Arg Ser Pro Gln Glu Arg Asn Thr Arg 85 90 95 Leu Glu Asn Met Thr Trp Arg Ile Trp Asn Leu Ala Arg Lys Lys Lys 100 105 110 Glu Xaa Glu Ala Glu Glu Ala Asn Arg Leu Leu Lys Arg Arg Leu Glu 115 120 125 Thr Glu Lys Pro Arg Thr Asp Ala Ala Ala Glu Met Ser Glu Asp Leu 130 135 140 Phe Glu Gly Gln Lys Gly Glu Asp Ala Gly Asp Ala Ser Val Ala Tyr 145 150 155 160 Gly Asp Ser Ser Ala Ser Asn Thr Pro Arg Ile Ser Ser Ile Asp Lys 165 170 175 Leu Tyr Ile Val Leu Ile Ser Leu His Gly Leu Val Arg Gly Glu Asn 180 185 190 Met Glu Leu Gly Arg Asp Ser Asp Thr Ser Gly Gln Val Lys Tyr Val 195 200 205 Val Glu Leu Ala Lys Ala Leu Ser Ser Cys Pro Gly Val Tyr Arg Val 210 215 220 Asp Leu Leu Thr Arg Gln Ile Leu Ala Pro Asn Tyr Asp Arg Gly Tyr 225 230 235 240 Gly Glu Pro Ser Glu Thr Leu Leu Pro Thr Asn Leu Lys Asn Phe Lys 245 250 255 His Glu Arg Gly Glu Asn Ser Gly Ala Tyr Ile Thr Arg Ile Pro Phe 260 265 270 Gly Pro Lys Asp Lys Tyr Leu Ala Lys Glu Gln Leu Trp Pro Tyr Val 275 280 285 Gln Glu Phe Val Asp Gly Ala Leu Ser His Ile Val Arg Met Ser Lys 290 295 300 Thr Ile Gly Glu Glu Ile Gly Cys Gly His Pro Met Trp Pro Ala Ala 305 310 315 320 Ile His Gly His Tyr Ala Ser Ala Gly Val Ala Ala Ala Leu Leu Ser 325 330 335 Gly Ala Leu Asn Val His Met Ile Phe Thr Gly His Phe Leu Gly Arg 340 345 350 Asp Lys Leu Glu Gly Leu Leu Lys Gln Gly Lys Gln Thr Arg Glu Glu 355 360 365 Ile Asn Met Thr Tyr Lys Ile Met Arg Arg Ile Glu Ala Glu Glu Leu 370 375 380 Ser Leu Asp Ala Ser Glu Ile Val Ile Ala Ser Thr Arg Gln Glu Ile 385 390 395 400 Glu Glu Gln Trp Asn Leu Tyr Asp Gly Phe Glu Val Met Leu Ala Arg 405 410 415 Lys Leu Arg Ala Arg Val Lys Arg Gly Ala Asn Cys Tyr Gly Arg Tyr 420 425 430 Met Pro Arg Met Val Ile Ile Pro Pro Gly Val Glu Phe Gly His Met 435 440 445 Ile Gln Asp Phe Asp Met Asp Gly Glu Glu Asp Ser Pro Ser Pro Ala 450 455 460 Ser Glu Asp Pro Pro Ile Trp Ser Glu Ile Met Arg Phe Phe Thr Asn 465 470 475 480 Pro Arg Lys Pro Leu Ile Leu Ala Val Ala Arg Pro Tyr Pro Glu Lys 485 490 495 Asn Ile Thr Thr Leu Val Arg Ala Phe Gly Glu Cys Arg Pro Leu Arg 500 505 510 Glu Leu Ala Asn Leu Thr Leu Ile Met Gly Asn Arg Glu Ala Ile Ser 515 520 525 Lys Met Ser Asn Met Ser Ala Ala Val Leu Thr Ser Val Leu Thr Leu 530 535 540 Ile Asp Glu Tyr Asp Leu Tyr Gly Gln Val Ala Tyr Pro Lys His His 545 550 555 560 Lys His Ser Glu Val Leu Asp Ile Tyr Arg Leu Ala Ala Arg Thr Lys 565 570 575 Gly Ala Phe Val Asn Val Ala Tyr Phe Glu Gln Phe Gly Val Thr Leu 580 585 590 Ile Glu Ala Ala Met His Gly Leu Pro Val Ile Ala Thr Lys Asn Gly 595 600 605 Ala Pro Val Glu Ile His Gln Val Leu Asn Asn Gly Leu Leu Val Asp 610 615 620 Pro His Asp Gln Asn Ala Ile Ala Asp Ala Leu Tyr Lys Leu Leu Ser 625 630 635 640 Glu Lys Gln Leu Trp Ser Arg Cys Arg Glu Asn Gly Leu Lys Asn Ile 645 650 655 His Gln Phe Ser Trp Pro Glu His Cys Lys Asn Tyr Leu Ser Arg Ile 660 665 670 Leu Thr Leu Ser Pro Arg Tyr Pro Ala Phe Ala Ser Asn Asp Asp Gln 675 680 685 Ile Lys Ala Pro Ile Lys Gly Arg Lys Tyr Ile Ile Val Ile Ala Val 690 695 700 Asp Ser Ala Ser Lys Lys Asp Leu Ala Phe Ile Ile Arg Asn Ser Ile 705 710 715 720 Glu Ala Thr Arg Thr Glu Thr Ser Ser Gly Ser Thr Gly Phe Val Leu 725 730 735 Ser Thr Ser Leu Thr Ile Ser Glu Ile His Ser Leu Leu Ile Ser Ala 740 745 750 Gly Met Val Pro Thr Asp Phe Asp Ala Phe Ile Cys Asn Ser Gly Ser 755 760 765 Asp Leu Phe Tyr Pro Ser Gln Thr Gly Asp Ser Pro Ser Thr Ser Arg 770 775 780 Val Thr Phe Ala Leu Asp Arg Asn Tyr Gln Ser Arg Val Glu Tyr His 785 790 795 800 Trp Gly Gly Glu Gly Leu Arg Lys Tyr Leu Val Lys Trp Ala Ser Ser 805 810 815 Val Val Glu Arg Arg Gly Arg Met Glu Lys Gln Val Ile Phe Asp Asp 820 825 830 Ser Glu His Ser Ser Thr Cys Cys Leu Ala Phe Arg Val Val Asn Pro 835 840 845 Asn Tyr Leu Pro Pro Leu Lys Glu Leu Gln Lys Leu Met Arg Val Gln 850 855 860 Ser Leu Arg Cys His Ala Leu Tyr Asn His Ser Ala Thr Arg Leu Ser 865 870 875 880 Val Ile Pro Ile His Ala Ser Arg Ser Gln Ala Ile Arg Tyr Leu Ser 885 890 895 Val Arg Trp Gly Ile Glu Leu Pro Asn Val Val Ile Leu Val Gly Glu 900 905 910 Ser Gly Asp Ser Asp Tyr Glu Glu Leu Phe Gly Gly Leu His Lys Thr 915 920 925 Val Val Leu Asn Gly Glu Phe Asn Thr Pro Ala Asn Arg Ile His Thr 930 935 940 Val Arg Arg Tyr Pro Leu Gln Asp Val Ile Ala Leu Asp Cys Ser Asn 945 950 955 960 Ile Val Gly Val Gln Gly Cys Ser Thr Asp Cys Met Arg Ser Thr Leu 965 970 975 Glu Lys Leu Gly Ile Pro Thr Lys 980 66 522 PRT Festuca arundinacea 66 Met Val Arg Gly Gly Gly Asn Gly Glu Val Glu Leu Ser Val Gly Ala 1 5 10 15 Gly Gly Gly Gly Gly Gly Ala Gly Gly Leu Val Glu Pro Pro Val Pro 20 25 30 Ile Ser Leu Gly Arg Leu Val Leu Ala Gly Met Val Ala Gly Gly Val 35 40 45 Gln Tyr Gly Trp Ala Leu Gln Leu Ser Leu Leu Thr Pro Tyr Val Gln 50 55 60 Thr Leu Gly Leu Ser His Ala Leu Thr Ser Phe Met Trp Leu Cys Gly 65 70 75 80 Pro Ile Ala Gly Leu Val Val Gln Pro Cys Val Gly Leu Tyr Ser Asp 85 90 95 Lys Cys Thr Ser Arg Trp Gly Arg Arg Arg Pro Phe Ile Met Thr Gly 100 105 110 Cys Val Leu Ile Cys Ile Ala Val Val Ile Val Gly Phe Ser Ala Asp 115 120 125 Ile Gly Ala Ala Leu Gly Asp Ser Lys Glu Glu Cys Ser Leu Tyr His 130 135 140 Gly Pro Arg Trp His Ala Ala Ile Val Tyr Val Leu Gly Phe Trp Leu 145 150 155 160 Leu Asp Phe Ser Asn Asn Thr Val Gln Gly Pro Ala Arg Ala Leu Met 165 170 175 Ala Asp Leu Ser Gly Lys Tyr Gly Pro Ser Ala Ala Asn Ser Ile Phe 180 185 190 Cys Ser Trp Met Ala Leu Gly Asn Ile Leu Gly Tyr Ser Ser Gly Ser 195 200 205 Thr Asp Lys Trp His Lys Trp Phe Pro Phe Leu Arg Thr Arg Ala Cys 210 215 220 Cys Glu Ala Cys Ala Asn Leu Lys Gly Ala Phe Leu Val Ala Val Leu 225 230 235 240 Phe Leu Cys Met Cys Leu Val Ile Thr Leu Ile Phe Ala Lys Glu Val 245 250 255 Pro Tyr Lys Arg Ile Ala Pro Leu Pro Thr Lys Ala Asn Gly Gln Val 260 265 270 Glu Val Glu Pro Ser Gly Pro Leu Ala Val Phe Gln Gly Ile Arg Asn 275 280 285 Leu Pro Ser Gly Met Pro Ser Val Leu Leu Val Thr Gly Leu Thr Trp 290 295 300 Leu Ser Trp Phe Pro Phe Ile Leu Tyr Asp Thr Asp Trp Met Gly Arg 305 310 315 320 Glu Ile Tyr His Gly Asp Pro Lys Gly Thr Pro Ala Glu Met Ser Ala 325 330 335 Phe Gln Asp Gly Val Arg Ala Gly Ala Phe Gly Leu Leu Leu Asn Ser 340 345 350 Ile Ile Leu Gly Phe Ser Ser Phe Leu Ile Glu Pro Met Cys Lys Arg 355 360 365 Leu Gly Pro Arg Val Val Trp Val Ser Ser Asn Phe Leu Val Cys Ile 370 375 380 Ala Met Ala Ala Thr Ala Ile Ile Ser Trp Trp Ser Thr Lys Glu Phe 385 390 395 400 His Glu Tyr Val Gln His Ala Ile Thr Ala Ser Lys Asp Ile Lys Ile 405 410 415 Val Cys Met Ala Leu Phe Ala Phe Leu Gly Val Pro Leu Ala Ile Leu 420 425 430 Tyr Ser Val Pro Phe Ala Val Thr Ala Gln Leu Ala Ala Ser Lys Gly 435 440 445 Gly Gly Gln Gly Leu Cys Thr Gly Val Leu Asn Ile Ser Ile Val Ile 450 455 460 Pro Gln Val Ile Ile Ala Leu Gly Ala Gly Pro Trp Asp Gln Leu Phe 465 470 475 480 Gly Lys Gly Asn Ile Pro Ala Phe Ala Ala Ala Ser Ala Phe Ala Leu 485 490 495 Ile Gly Gly Ile Val Gly Ile Phe Leu Leu Pro Lys Ile Ser Arg Arg 500 505 510 Ser Phe Arg Ala Val Ser Thr Gly Gly His 515 520 67 407 PRT Festuca arundinacea 67 Ile Cys Val Ala Val Val Val Val Gly Phe Ser Ala Asp Ile Gly Ala 1 5 10 15 Ala Leu Gly Asp Ser Lys Glu Glu Cys Ser Leu Tyr His Gly Pro Arg 20 25 30 Trp His Ala Ala Ile Val Tyr Val Leu Gly Phe Trp Leu Leu Asp Phe 35 40 45 Ser Asn Asn Thr Val Gln Gly Pro Ala Arg Ala Leu Met Ala Asp Leu 50 55 60 Ser Gly Lys Tyr Gly Pro Ser Ala Ala Asn Ser Ile Phe Cys Ser Trp 65 70 75 80 Met Ala Leu Gly Asn Ile Leu Gly Tyr Ser Ser Gly Ser Thr Asp Lys 85 90 95 Trp His Lys Trp Phe Pro Phe Leu Arg Thr Arg Ala Cys Cys Glu Ala 100 105 110 Cys Ala Asn Leu Lys Gly Ala Phe Leu Val Ala Val Leu Phe Leu Cys 115 120 125 Phe Cys Leu Val Ile Thr Leu Ile Phe Ala Lys Glu Val Pro Tyr Lys 130 135 140 Arg Ile Ala Pro Leu Pro Thr Lys Ala Asn Gly Gln Val Glu Val Glu 145 150 155 160 Pro Ser Gly Pro Leu Ala Val Phe Gln Gly Phe Arg Asn Leu Pro Ser 165 170 175 Gly Met Pro Ser Val Leu Leu Val Thr Gly Leu Thr Trp Leu Ser Trp 180 185 190 Phe Pro Phe Ile Leu Tyr Asp Thr Asp Trp Met Gly Arg Glu Ile Tyr 195 200 205 His Gly Asp Pro Lys Gly Thr Pro Ala Glu Ala Ser Ala Phe Gln Asp 210 215 220 Gly Val Arg Ala Gly Ala Phe Gly Leu Leu Leu Asn Ser Ile Ile Leu 225 230 235 240 Gly Phe Ser Ser Phe Leu Ile Glu Pro Met Cys Lys Arg Leu Gly Pro 245 250 255 Arg Val Val Trp Val Ser Ser Asn Leu Leu Val Cys Ile Ala Met Ala 260 265 270 Ala Thr Ala Ile Ile Ser Trp Trp Ser Thr Lys Glu Phe His Glu Tyr 275 280 285 Val Gln His Ala Ile Thr Ala Ser Lys Asp Ile Lys Ile Val Cys Met 290 295 300 Val Leu Phe Ala Phe Leu Gly Val Pro Leu Ala Ile Leu Tyr Ser Val 305 310 315 320 Pro Phe Ala Val Thr Ala Gln Leu Ala Ala Asn Lys Gly Gly Gly Gln 325 330 335 Gly Leu Cys Thr Gly Val Leu Asn Ile Ser Ile Val Ile Pro Gln Val 340 345 350 Ile Ile Ala Leu Gly Ala Gly Pro Trp Asp Gln Leu Phe Gly Lys Gly 355 360 365 Asn Ile Pro Ala Phe Ala Ala Ala Ser Ala Phe Ala Leu Ile Gly Gly 370 375 380 Ile Val Gly Ile Phe Leu Leu Pro Lys Ile Ser Arg His Ser Phe Arg 385 390 395 400 Ala Val Ser Thr Gly Gly His 405 68 522 PRT Festuca arundinacea 68 Met Val Arg Gly Gly Gly Asn Ser Glu Val Glu Leu Ser Val Gly Ala 1 5 10 15 Gly Gly Gly Gly Gly Gly Ala Gly Gly Leu Val Glu Pro Pro Val Pro 20 25 30 Ile Ser Leu Gly Arg Leu Val Phe Ala Gly Met Val Ala Gly Gly Val 35 40 45 Gln Tyr Gly Trp Ala Leu Gln Leu Ser Leu Leu Thr Pro Tyr Val Gln 50 55 60 Thr Leu Gly Leu Ser His Ala Leu Thr Ser Phe Met Trp Leu Cys Gly 65 70 75 80 Pro Ile Ala Gly Leu Val Val Gln Pro Cys Val Gly Leu Tyr Ser Asp 85 90 95 Lys Cys Thr Ser Arg Trp Gly Arg Arg Arg Pro Phe Ile Met Thr Gly 100 105 110 Cys Val Leu Ile Cys Ile Ala Val Val Ile Val Gly Phe Ser Ala Asp 115 120 125 Ile Gly Ala Ala Leu Gly Asp Ser Lys Glu Glu Cys Ser Leu Tyr His 130 135 140 Gly Pro Arg Trp His Ala Ala Ile Val Tyr Val Leu Gly Phe Trp Leu 145 150 155 160 Leu Asp Phe Ser Asn Asn Thr Val Gln Gly Pro Ala Arg Ala Leu Met 165 170 175 Ala Asp Leu Ser Gly Lys Tyr Gly Pro Ser Ala Ala Asn Ser Ile Phe 180 185 190 Cys Ser Trp Met Ala Leu Gly Asn Ile Leu Gly Tyr Ser Ser Gly Ser 195 200 205 Thr Asp Lys Trp His Lys Trp Phe Pro Phe Leu Arg Thr Arg Ala Cys 210 215 220 Cys Glu Ala Cys Ala Asn Leu Lys Gly Ala Phe Leu Val Ala Val Leu 225 230 235 240 Phe Leu Cys Phe Cys Leu Val Ile Thr Leu Ile Phe Ala Lys Glu Val 245 250 255 Pro Tyr Lys Arg Ile Ala Pro Leu Pro Thr Lys Ala Asn Gly Gln Val 260 265 270 Glu Val Glu Pro Ser Gly Pro Leu Ala Val Phe Gln Gly Phe Arg Asn 275 280 285 Leu Pro Ser Gly Met Pro Ser Val Leu Leu Val Thr Gly Leu Thr Trp 290 295 300 Leu Ser Trp Phe Pro Phe Ile Leu Tyr Asp Thr Asp Trp Met Gly Arg 305 310 315 320 Glu Ile Tyr His Gly Asp Pro Lys Gly Thr Pro Ala Glu Ala Ser Ala 325 330 335 Phe Gln Asp Gly Val Arg Ala Gly Ala Phe Gly Leu Leu Leu Asn Ser 340 345 350 Ile Ile Leu Gly Phe Ser Ser Phe Leu Ile Glu Pro Met Cys Lys Arg 355 360 365 Leu Gly Pro Arg Val Val Trp Val Ser Ser Asn Leu Leu Val Cys Ile 370 375 380 Ala Met Ala Ala Thr Ala Ile Ile Ser Trp Trp Ser Thr Lys Glu Phe 385 390 395 400 His Glu Tyr Val Gln His Ala Ile Thr Ala Ser Lys Asp Ile Lys Ile 405 410 415 Val Cys Met Val Leu Phe Ala Phe Leu Gly Val Pro Leu Ala Ile Leu 420 425 430 Tyr Ser Val Pro Phe Ala Val Thr Ala Gln Leu Ala Ala Asn Lys Gly 435 440 445 Gly Gly Gln Gly Leu Cys Thr Gly Val Leu Asn Ile Ser Ile Val Ile 450 455 460 Pro Gln Val Ile Ile Ala Leu Gly Ala Gly Pro Trp Asp Gln Leu Phe 465 470 475 480 Gly Lys Gly Asn Ile Pro Ala Phe Ala Ala Ala Ser Ala Phe Ala Leu 485 490 495 Ile Gly Gly Ile Val Gly Ile Phe Leu Leu Pro Lys Ile Ser Arg His 500 505 510 Ser Phe Arg Ala Val Ser Thr Gly Gly His 515 520 69 506 PRT Lolium perenne 69 Met Pro Pro Pro Arg Arg Pro Thr Thr Gly Gly Thr Thr Thr Thr Ser 1 5 10 15 Ala Ala Leu Pro Pro Pro Arg Lys Val Pro Leu Arg Ser Leu Leu Arg 20 25 30 Ala Ala Ser Val Ala Cys Gly Val Gln Phe Gly Trp Ala Leu Gln Leu 35 40 45 Ser Leu Leu Thr Pro Tyr Val Gln Glu Leu Gly Ile Pro His Ala Phe 50 55 60 Ala Ser Leu Val Trp Leu Cys Gly Pro Leu Ser Gly Leu Leu Val Gln 65 70 75 80 Pro Leu Ile Gly His Leu Ser Asp Arg Ile Ala Pro Ala Asp Ser Pro 85 90 95 Leu Gly Arg Arg Arg Pro Phe Ile Ala Ala Gly Ala Ala Ser Ile Ala 100 105 110 Phe Ser Val Leu Thr Val Gly Phe Ser Ala Asp Leu Gly Arg Leu Phe 115 120 125 Gly Asp Asn Val Arg Pro Gly Ser Thr Arg Tyr Gly Ala Ile Ile Val 130 135 140 Tyr Met Ile Gly Phe Trp Leu Leu Asp Val Gly Asn Asn Ala Thr Gln 145 150 155 160 Gly Pro Cys Arg Ala Phe Leu Ala Asp Leu Thr Glu Asn Asp Pro Arg 165 170 175 Arg Thr Arg Ile Ala Asn Ala Tyr Phe Ser Leu Phe Met Ala Leu Gly 180 185 190 Asn Ile Leu Gly Tyr Ala Thr Gly Ala Tyr Ser Gly Trp Tyr Lys Ile 195 200 205 Phe Pro Phe Thr Ile Thr Glu Ser Cys Gly Val Ser Cys Ala Asn Leu 210 215 220 Lys Ser Ala Phe Leu Leu Asp Ile Ile Ile Leu Ala Ile Thr Thr Tyr 225 230 235 240 Val Thr Val Val Thr Val Gln Asp Asn Pro Thr Phe Gly Ser Asp Glu 245 250 255 Ala Ala Pro Arg Pro Ser Ser His Glu Glu Glu Ala Phe Leu Phe Glu 260 265 270 Leu Phe Gly Ser Phe Lys Tyr Phe Thr Met Pro Val Trp Met Val Leu 275 280 285 Ile Val Thr Ser Leu Thr Trp Ile Gly Trp Phe Pro Phe Ile Leu Phe 290 295 300 Asp Thr Asp Trp Met Gly Arg Glu Ile Tyr Arg Gly Ser Pro Glu Ile 305 310 315 320 Val Ala Asp Thr Gln Lys Tyr His Asp Gly Val Arg Met Gly Ser Phe 325 330 335 Gly Leu Met Leu Asn Ser Val Leu Leu Gly Ile Thr Ser Val Val Thr 340 345 350 Glu Lys Leu Cys Arg Lys Trp Gly Ala Gly Leu Val Trp Gly Val Ser 355 360 365 Asn Ile Ile Met Ala Leu Cys Phe Val Ala Met Leu Val Ile Thr Tyr 370 375 380 Val Ala Gln Asn Leu Asp Tyr Gly Pro Ser Gly Ala Pro Pro Thr Gly 385 390 395 400 Ile Val Val Ala Ser Leu Thr Val Phe Thr Ile Leu Gly Ala Pro Leu 405 410 415 Ser Ile Thr Tyr Ser Ile Pro Tyr Ala Met Ala Thr Ser Arg Val Glu 420 425 430 Asn Leu Gly Leu Gly Gln Gly Leu Ala Met Gly Ile Leu Asn Leu Ser 435 440 445 Ile Val Ile Pro Gln Ile Ile Val Ser Leu Gly Ser Gly Pro Trp Asp 450 455 460 Ser Leu Phe Gly Gly Gly Asn Ala Pro Ser Phe Trp Val Ala Ala Ala 465 470 475 480 Ala Ser Phe Ile Gly Gly Leu Val Ala Ile Leu Gly Leu Pro Arg Ala 485 490 495 Arg Ile Ala Pro Lys Lys Arg Ser Gln Arg 500 505 70 504 PRT Festuca arundinacea 70 Met Pro Pro Pro Arg Arg Pro Asn Ala Gly Gly Thr Thr Ser Ala Pro 1 5 10 15 Leu Pro Pro Pro Arg Lys Val Pro Leu Arg Ser Leu Leu Arg Ala Ala 20 25 30 Ser Val Ala Cys Gly Val Gln Phe Gly Trp Ala Leu Gln Leu Ser Leu 35 40 45 Leu Thr Pro Tyr Val Gln Glu Leu Gly Ile Pro His Ala Phe Ala Ser 50 55 60 Leu Val Trp Leu Cys Gly Pro Leu Ser Gly Leu Leu Val Gln Pro Leu 65 70 75 80 Ile Gly His Leu Ser Asp Arg Ile Ala Pro Ala Asp Ser Pro Leu Gly 85 90 95 Arg Arg Arg Pro Phe Ile Ala Ala Gly Ala Ala Ser Ile Ala Phe Ser 100 105 110 Val Leu Thr Val Gly Phe Ser Ala Asp Leu Gly Arg Leu Phe Gly Asp 115 120 125 Asn Ile Arg Pro Gly Ser Thr Arg Phe Gly Ala Ile Ile Val Tyr Met 130 135 140 Ile Gly Phe Trp Leu Leu Asp Val Gly Asn Asn Ala Thr Gln Gly Pro 145 150 155 160 Cys Arg Ala Phe Leu Ala Asp Leu Thr Glu Asn Asp Pro Arg Arg Thr 165 170 175 Arg Ile Ala Asn Ala Tyr Phe Ser Leu Phe Met Ala Leu Gly Asn Ile 180 185 190 Leu Gly Tyr Ala Thr Gly Ala Tyr Ser Gly Trp Tyr Lys Ile Phe Pro 195 200 205 Phe Thr Ile Thr Glu Ser Cys Gly Val Ser Cys Ala Asn Leu Lys Ser 210 215 220 Ala Phe Leu Leu Asp Ile Ile Ile Leu Ala Ile Thr Thr Tyr Val Thr 225 230 235 240 Val Val Thr Val Gln Asp Asn Pro Thr Phe Gly Ser Asp Glu Ala Ala 245 250 255 Pro Arg Pro Ser Ser His Glu Glu Glu Ala Phe Leu Phe Glu Leu Phe 260 265 270 Gly Ser Phe Lys Tyr Phe Thr Leu Pro Val Trp Met Val Leu Ile Val 275 280 285 Thr Ser Leu Thr Trp Ile Gly Trp Phe Pro Phe Ile Leu Phe Asp Thr 290 295 300 Asp Trp Met Gly Arg Glu Ile Tyr Arg Gly Ser Pro Glu Ile Val Ala 305 310 315 320 Asp Thr Gln Lys Tyr His Asp Gly Val Arg Met Gly Ser Phe Gly Leu 325 330 335 Met Leu Asn Ser Val Leu Leu Gly Ile Thr Ser Val Val Met Glu Lys 340 345 350 Leu Cys Arg Lys Trp Gly Ala Gly Leu Val Trp Gly Val Ser Asn Ile 355 360 365 Ile Met Ala Leu Cys Phe Val Ala Met Leu Ile Ile Thr Tyr Val Ala 370 375 380 Lys Asn Leu Asp Tyr Gly Pro Ser Gly Ala Pro Pro Thr Gly Ile Val 385 390 395 400 Val Ala Ser Leu Ala Val Phe Thr Ile Leu Gly Ala Pro Leu Ser Ile 405 410 415 Thr Tyr Ser Ile Pro Tyr Ala Met Ala Thr Ser Arg Val Glu Asn Leu 420 425 430 Gly Leu Gly Gln Gly Leu Ala Met Gly Ile Leu Asn Leu Ser Ile Val 435 440 445 Ile Pro Gln Ile Ile Val Ser Leu Gly Ser Gly Pro Trp Asp Ser Leu 450 455 460 Phe Gly Gly Gly Asn Ala Pro Ser Phe Trp Val Ala Ala Ala Ala Ser 465 470 475 480 Phe Ile Gly Gly Leu Val Ala Ile Leu Gly Leu Pro Arg Ala Arg Ile 485 490 495 Ala Pro Lys Lys Arg Ser Gln Arg 500 71 508 PRT Lolium perenne 71 Met Val Asp Gln Asp His Asp Gly Arg Arg Arg Gln Glu Glu Ala Thr 1 5 10 15 Ala Val Ala Ala Ser Ser Val Pro Leu Leu Glu Lys Lys Pro Gly Asp 20 25 30 Val Pro Tyr Tyr Val Glu Gly Cys Pro Gly Cys Ala Val Asp Arg Arg 35 40 45 Lys Ala Thr Asp Pro Gly Ile Pro Tyr Gly Ser Phe Ile Tyr Ile Trp 50 55 60 Val Val Ile Leu Cys Thr Ala Ile Pro Ile Ser Ser Leu Phe Pro Phe 65 70 75 80 Leu Tyr Phe Met Ile Arg Asp Leu His Ile Ala Glu Arg Thr Glu Asp 85 90 95 Ile Gly Phe Tyr Ala Gly Phe Val Gly Ala Ala Phe Met Phe Gly Arg 100 105 110 Cys Leu Thr Ser Thr Ile Trp Gly Ile Ala Ala Asp Arg Ile Gly Arg 115 120 125 Lys Pro Val Val Ile Phe Gly Val Phe Ser Val Val Ile Phe Asn Ala 130 135 140 Leu Phe Gly Leu Ser Val Thr Tyr Trp Met Ala Ile Ala Thr Arg Phe 145 150 155 160 Leu Leu Gly Ala Leu Asn Gly Leu Leu Gly Pro Met Lys Ala Tyr Ala 165 170 175 Ile Glu Val Cys Arg Pro Glu His Glu Ala Leu Ala Leu Ser Leu Val 180 185 190 Ser Thr Ala Trp Gly Ile Gly Leu Ile Ile Gly Pro Ala Leu Gly Gly 195 200 205 Tyr Leu Ala Leu Pro Ala Glu Lys Tyr Pro Asn Ile Phe Ser Pro Asp 210 215 220 Ser Leu Phe Gly Arg Phe Pro Tyr Phe Leu Pro Cys Leu Cys Thr Ser 225 230 235 240 Val Phe Ala Ala Ala Val Leu Ile Gly Cys Ile Trp Met Pro Glu Thr 245 250 255 Leu His Lys His Lys Val Asn Glu Asn Arg Asn Gln Ser Val Glu Ser 260 265 270 Leu Glu Ala His Leu Ile Asp Pro Lys Glu Lys Val Glu Gln Ser Asn 275 280 285 Ser Pro Asp Thr Lys Lys Ser Leu Phe Lys Asn Trp Pro Leu Met Ser 290 295 300 Ser Ile Ile Val Tyr Cys Val Phe Ser Phe His Asp Met Ala Tyr Thr 305 310 315 320 Glu Val Phe Ser Leu Trp Ala Glu Ser Asp Arg Thr Tyr Gly Gly Leu 325 330 335 Ser Leu Ser Ser Glu Asp Val Gly Gln Thr Leu Ala Ile Thr Gly Ser 340 345 350 Ser Leu Leu Val Tyr Gln Leu Phe Leu Tyr Pro Arg Ile Asn Arg Val 355 360 365 Leu Gly Pro Ile Lys Ser Ser Gln Ile Ala Ala Gly Ile Cys Ile Pro 370 375 380 Ile Leu Phe Ala Tyr Pro Tyr Met Thr Tyr Leu Ser Glu Pro Gly Leu 385 390 395 400 Ser Ile Val Leu Asn Ile Ala Ser Val Ile Lys Asn Asn Leu Gly Val 405 410 415 Thr Ile Ile Thr Gly Thr Phe Ile Leu Gln Asn Asn Ala Val Pro Gln 420 425 430 Asp Gln Arg Gly Ala Ala Asn Gly Leu Ala Met Thr Gly Met Ser Phe 435 440 445 Phe Lys Ala Val Ala Pro Ala Gly Ala Gly Ile Val Phe Ser Trp Ala 450 455 460 Gln Lys Arg Gln His Ala Phe Phe Phe Pro Gly Asp Gln Met Val Phe 465 470 475 480 Phe Leu Leu Asn Ile Ile Glu Leu Leu Gly Leu Leu Leu Thr Phe Lys 485 490 495 Phe Phe Leu Ala Val Pro Asp Lys Ser Asp Ser Asn 500 505 72 522 PRT Lolium perenne 72 Met Ser Ser Met Gln Phe Ser Ser Val Leu Pro Leu Glu Gly Lys Ala 1 5 10 15 Cys Val Cys Pro Val Arg Ser Ala Asn Asn Gly Cys Glu Arg Leu Lys 20 25 30 Val Gly Asp Ser Ser Ser Leu Arg His Glu Met Ala Leu Arg Arg Lys 35 40 45 Cys Asn Gly Ala Arg Gly Gly Gly Ala Ala Asn Gly Ala Gln Cys Val 50 55 60 Leu Thr Ser Asp Ala Ser Pro Asp Thr Leu Val Val Arg Ser Ser Phe 65 70 75 80 Arg Arg Asn Tyr Ala Asp Pro Asn Glu Val Ala Ala Val Ile Leu Gly 85 90 95 Gly Gly Thr Gly Thr Gln Leu Phe Pro Leu Thr Ser Thr Arg Ala Thr 100 105 110 Pro Ala Val Pro Ile Gly Gly Cys Tyr Arg Leu Ile Asp Ile Pro Met 115 120 125 Ser Asn Cys Phe Asn Ser Gly Ile Asn Lys Ile Phe Val Met Thr Gln 130 135 140 Phe Asn Ser Ala Ser Leu Asn Arg His Ile His Arg Thr Tyr Leu Gly 145 150 155 160 Gly Gly Ile Asn Phe Thr Asp Gly Ser Val Glu Val Leu Ala Ala Thr 165 170 175 Gln Met Pro Gly Glu Ala Ala Gly Trp Phe Arg Gly Thr Ala Asp Ala 180 185 190 Val Arg Lys Phe Ile Trp Val Leu Glu Asp Tyr Tyr Lys His Lys Ser 195 200 205 Ile Glu His Ile Leu Ile Leu Ser Gly Asp Gln Leu Tyr Arg Met Asp 210 215 220 Tyr Met Glu Leu Val Gln Lys His Val Asp Asp Asn Ala Asp Ile Thr 225 230 235 240 Leu Ser Cys Ala Pro Val Gly Glu Ser Arg Ala Ser Glu Tyr Gly Leu 245 250 255 Val Lys Phe Asp Ser Ser Gly Arg Val Ile Gln Phe Ser Glu Lys Pro 260 265 270 Lys Gly Ala Asp Leu Glu Ala Met Lys Val Asp Thr Ser Phe Leu Asn 275 280 285 Phe Ala Ile Asp Asp Pro Ala Lys Asn Pro Tyr Ile Ala Ser Met Gly 290 295 300 Val Tyr Val Phe Lys Arg Glu Val Leu Leu Asn Leu Leu Lys Ser Arg 305 310 315 320 Tyr Thr Glu Leu His Asp Phe Gly Ser Glu Ile Leu Pro Arg Ala Leu 325 330 335 His Asp His Asn Val Gln Ala Tyr Val Phe Thr Asp Tyr Trp Glu Asp 340 345 350 Ile Gly Thr Ile Arg Ser Phe Phe Asp Ala Asn Met Ala Leu Cys Glu 355 360 365 Gln Pro Pro Lys Phe Glu Phe Tyr Asp Pro Lys Thr Pro Phe Phe Thr 370 375 380 Ser Pro Arg Tyr Leu Pro Pro Thr Lys Ser Asp Lys Cys Arg Ile Lys 385 390 395 400 Glu Ala Ile Ile Ser His Gly Cys Phe Leu Arg Glu Cys Thr Ile Glu 405 410 415 His Ser Ile Ile Gly Val Arg Ser Arg Leu Asn Ser Gly Ser Val Leu 420 425 430 Lys Asn Ala Met Met Met Gly Ala Asp Leu Tyr Glu Thr Glu Asp Glu 435 440 445 Ile Ser Gly Leu Leu Ser Glu Gly Lys Val Pro Ile Gly Val Gly Glu 450 455 460 Asn Ser Lys Leu Ser Asn Cys Ile Ile Asp Met Asn Ala Arg Ile Gly 465 470 475 480 Arg Asp Val Val Ile Ala Asn Ser Glu Gly Val Gln Glu Ala Asp Arg 485 490 495 Pro Glu Glu Gly Tyr Tyr Ile Arg Ser Gly Ile Val Val Ile Leu Lys 500 505 510 Asn Ala Thr Val Lys Asp Gly Thr Val Val 515 520 73 522 PRT Festuca arundinacea 73 Met Ser Ser Met Gln Phe Ser Ser Val Leu Pro Leu Glu Gly Lys Ala 1 5 10 15 Cys Val Cys Pro Val Arg Ser Ala Asn Asn Gly Cys Glu Arg Leu Lys 20 25 30 Val Gly Asp Ser Ser Ser Leu Arg His Glu Met Ala Leu Arg Arg Lys 35 40 45 Cys Asn Gly Ala Arg Gly Gly Gly Ala Ala Asp Gly Ala Gln Cys Val 50 55 60 Leu Thr Ser Asp Ala Ser Pro Asp Thr Leu Val Val Arg Ser Ser Phe 65 70 75 80 Arg Met Asn Tyr Ala Asp Pro Asn Glu Val Ala Ala Val Ile Leu Gly 85 90 95 Gly Gly Thr Gly Thr Gln Leu Phe Pro Leu Thr Ser Thr Arg Ala Thr 100 105 110 Pro Ala Val Pro Ile Gly Gly Cys Tyr Arg Leu Ile Asp Ile Pro Met 115 120 125 Ser Asn Cys Phe Asn Ser Gly Ile Asn Lys Ile Phe Val Met Thr Gln 130 135 140 Phe Asn Ser Ala Ser Leu Asn Arg His Ile His Arg Thr Tyr Leu Gly 145 150 155 160 Gly Gly Ile Asn Phe Thr Asp Gly Ser Val Glu Val Leu Ala Ala Thr 165 170 175 Gln Met Pro Gly Glu Ala Ala Gly Trp Phe Arg Gly Thr Ala Asp Ala 180 185 190 Val Arg Lys Phe Ile Trp Val Leu Glu Asp Tyr Tyr Lys His Lys Ser 195 200 205 Ile Glu His Ile Leu Ile Leu Ser Gly Asp Gln Leu Tyr Arg Met Asp 210 215 220 Tyr Met Glu Leu Val Gln Lys His Val Asp Asp Asn Ala Asp Ile Thr 225 230 235 240 Leu Ser Cys Ala Pro Val Gly Glu Ser Arg Ala Ser Glu Tyr Gly Leu 245 250 255 Val Lys Phe Asp Ser Ser Gly Arg Val Ile Gln Phe Ser Glu Lys Pro 260 265 270 Lys Gly Ala Asp Leu Glu Ala Met Lys Val Asp Thr Ser Phe Leu Asn 275 280 285 Phe Ala Ile Asp Asp Pro Ala Lys Asn Pro Tyr Ile Ala Ser Met Gly 290 295 300 Val Tyr Val Phe Lys Arg Glu Val Leu Leu Asn Leu Leu Lys Ser Arg 305 310 315 320 Tyr Thr Glu Leu His Asp Phe Gly Ser Glu Ile Leu Pro Arg Ala Leu 325 330 335 His Asp His Asn Val Gln Ala Tyr Val Phe Thr Asp Tyr Trp Glu Asp 340 345 350 Ile Gly Thr Ile Arg Ser Phe Phe Asp Ala Asn Met Ala Leu Cys Glu 355 360 365 Gln Pro Pro Lys Phe Glu Phe Tyr Asp Pro Lys Thr Pro Phe Phe Thr 370 375 380 Ser Pro Arg Tyr Leu Pro Pro Thr Lys Ser Asp Lys Cys Arg Ile Lys 385 390 395 400 Glu Ala Ile Ile Ser His Gly Cys Phe Leu Arg Glu Cys Thr Ile Glu 405 410 415 His Ser Ile Ile Gly Val Arg Ser Arg Leu Asn Ser Gly Ser Val Leu 420 425 430 Lys Asn Ala Met Met Met Gly Ala Asp Leu Tyr Glu Thr Glu Asp Glu 435 440 445 Ile Ser Gly Leu Leu Ser Glu Gly Lys Val Pro Ile Gly Val Gly Glu 450 455 460 Asn Ser Lys Leu Ser Asn Cys Ile Ile Asp Met Asn Ala Arg Ile Gly 465 470 475 480 Arg Asp Val Val Ile Ala Asn Ser Glu Gly Val Gln Glu Ala Asp Arg 485 490 495 Pro Glu Glu Gly Tyr Tyr Ile Arg Ser Gly Ile Val Val Ile Leu Lys 500 505 510 Asn Ala Thr Val Lys Asp Gly Thr Val Val 515 520 74 525 PRT Lolium perenne 74 Met Thr Gly Ala Pro Pro Ser Thr Val Met Ala Met Gly Ala Ala Thr 1 5 10 15 Ser Pro Cys Lys Ile Leu Ser Ala Thr Gln Arg Ala Ser Thr Ala Ala 20 25 30 Ala Ser Ala Ser Thr Ser Arg Glu Ser Val Ser Leu Arg Ala Pro Arg 35 40 45 Gly Arg Arg Gln Arg Pro Arg Pro Arg Gly Leu Ala Leu Ser Leu Ala 50 55 60 Pro Ala Arg Arg Pro Phe Val Phe Ser Pro Arg Ala Val Ser Asp Ser 65 70 75 80 Lys Ser Ser Gln Thr Cys Leu Asp Pro Asp Ala Ser Thr Ser Val Leu 85 90 95 Gly Ile Ile Leu Gly Gly Gly Ala Gly Thr Arg Leu Tyr Pro Leu Thr 100 105 110 Lys Lys Arg Ala Lys Pro Ala Val Pro Leu Gly Ala Asn Tyr Arg Leu 115 120 125 Ile Asp Ile Pro Val Ser Asn Cys Leu Asn Ser Asn Ile Ser Lys Ile 130 135 140 Tyr Val Leu Thr Gln Phe Asn Ser Ala Ser Leu Asn Arg His Leu Ser 145 150 155 160 Arg Ala Tyr Gly Ser Asn Ile Gly Gly Tyr Lys Asn Glu Gly Phe Val 165 170 175 Glu Val Leu Ala Ala Gln Gln Ser Pro Asp Asn Pro Asn Trp Phe Gln 180 185 190 Gly Thr Ala Asp Ala Val Arg Gln Tyr Leu Trp Leu Phe Glu Glu His 195 200 205 Asn Val Met Glu Tyr Leu Ile Leu Ala Gly Asp His Leu Tyr Arg Met 210 215 220 Asp Tyr Glu Lys Phe Ile Gln Ala His Arg Glu Thr Asp Ala Asp Ile 225 230 235 240 Thr Val Ala Ala Leu Pro Met Asp Glu Glu Arg Ala Thr Ala Phe Gly 245 250 255 Leu Met Lys Ile Asp Glu Glu Gly Arg Ile Val Glu Phe Ala Glu Lys 260 265 270 Pro Lys Gly Glu Gln Leu Lys Ala Met Met Val Asp Thr Thr Ile Leu 275 280 285 Gly Leu Asp Asp Val Arg Ala Lys Glu Met Pro Tyr Ile Ala Ser Met 290 295 300 Gly Ile Tyr Val Ile Ser Lys His Val Met Leu Gln Leu Leu Arg Asp 305 310 315 320 Gln Phe Pro Gly Ala Asn Asp Phe Gly Ser Glu Val Ile Pro Gly Ala 325 330 335 Thr Ser Thr Gly Met Arg Val Gln Ala Tyr Leu Tyr Asp Gly Tyr Trp 340 345 350 Glu Asp Ile Gly Thr Ile Glu Ala Phe Tyr Asn Ala Asn Leu Gly Ile 355 360 365 Thr Lys Lys Pro Ile Pro Asp Phe Ser Phe Tyr Asp Arg Ser Ala Pro 370 375 380 Ile Tyr Thr Gln Pro Arg His Leu Pro Pro Ser Lys Val Leu Asp Ala 385 390 395 400 Asp Val Thr Asp Ser Val Ile Gly Glu Gly Cys Val Ile Lys Asn Cys 405 410 415 Lys Ile His His Ser Val Val Gly Leu Arg Ser Cys Ile Ser Glu Gly 420 425 430 Ala Ile Ile Glu Asp Thr Leu Leu Met Gly Ala Asp Tyr Tyr Glu Thr 435 440 445 Glu Ala Asp Lys Lys Leu Leu Ala Asp Lys Gly Gly Ile Pro Ile Gly 450 455 460 Ile Gly Lys Asn Ser His Ile Arg Arg Ala Ile Ile Asp Lys Asn Ala 465 470 475 480 Arg Ile Gly Asp Asn Val Lys Ile Ile Asn Val Asp Asn Val Gln Glu 485 490 495 Ala Ala Arg Glu Thr Asp Gly Tyr Phe Ile Lys Ser Gly Ile Val Thr 500 505 510 Val Ile Lys Asp Ala Leu Leu Pro Ser Gly Thr Val Ile 515 520 525 75 524 PRT Festuca arundinacea 75 Met Thr Arg Ala Pro Pro Ser Thr Val Met Ala Met Gly Ala Ala Thr 1 5 10 15 Ser Pro Cys Lys Ile Leu Ser Ala Thr Gln Arg Ala Ser Ala Ala Ala 20 25 30 Pro Ser Ala Ser Thr Ser Arg Glu Ser Val Cys Leu Leu Arg Ala Pro 35 40 45 Arg Gly Arg Arg Gln Arg Pro Arg Gly Leu Ala Leu Ser Leu Ala Pro 50 55 60 Ala Arg Arg Pro Phe Val Phe Ser Pro Arg Ala Val Ser Asp Ser Lys 65 70 75 80 Ser Ser Gln Thr Cys Leu Asp Pro Asp Ala Ser Thr Ser Val Leu Gly 85 90 95 Ile Ile Leu Gly Gly Gly Ala Gly Thr Arg Leu Tyr Pro Leu Thr Lys 100 105 110 Lys Arg Ala Lys Pro Ala Val Pro Leu Gly Ala Asn Tyr Arg Leu Ile 115 120 125 Asp Ile Pro Val Ser Asn Cys Leu Asn Ser Asn Ile Ser Lys Ile Tyr 130 135 140 Val Leu Thr Gln Phe Asn Ser Ala Ser Leu Asn Arg His Leu Ser Arg 145 150 155 160 Ala Tyr Gly Ser Asn Ile Gly Gly Tyr Lys Asn Glu Gly Phe Val Glu 165 170 175 Val Leu Ala Ala Gln Gln Ser Pro Asp Asn Pro Asn Trp Phe Gln Gly 180 185 190 Thr Ala Asp Ala Val Arg Gln Tyr Leu Trp Leu Phe Glu Glu His Asn 195 200 205 Val Met Glu Tyr Leu Ile Leu Ala Gly Asp His Leu Tyr Arg Met Asp 210 215 220 Tyr Glu Lys Phe Ile Gln Ala His Arg Glu Thr Asp Ala Asp Ile Thr 225 230 235 240 Val Ala Ala Leu Pro Met Asp Glu Glu Arg Ala Thr Ala Phe Gly Leu 245 250 255 Met Lys Ile Asp Glu Glu Gly Arg Ile Val Glu Phe Ala Glu Lys Pro 260 265 270 Lys Gly Glu Gln Leu Lys Ala Met Met Val Asp Thr Thr Ile Leu Gly 275 280 285 Leu Asp Asp Val Arg Ala Lys Glu Met Pro Tyr Ile Ala Ser Met Gly 290 295 300 Ile Tyr Val Ile Ser Lys His Val Met Leu Gln Leu Leu Arg Asp Gln 305 310 315 320 Phe Pro Gly Ala Asn Asp Phe Gly Ser Glu Val Ile Pro Gly Ala Thr 325 330 335 Ser Thr Gly Met Arg Val Gln Ala Tyr Leu Tyr Asp Gly Tyr Trp Glu 340 345 350 Asp Ile Gly Thr Ile Glu Ala Phe Tyr Asn Ala Asn Leu Gly Ile Thr 355 360 365 Lys Lys Pro Ile Pro Asp Phe Ser Phe Tyr Asp Arg Ser Ala Pro Ile 370 375 380 Tyr Thr Gln Pro Arg His Leu Pro Pro Ser Lys Val Leu Asp Ala Asp 385 390 395 400 Val Thr Asp Ser Val Ile Gly Glu Gly Cys Val Ile Lys Asn Cys Lys 405 410 415 Ile His His Ser Val Val Gly Leu Arg Ser Cys Ile Ser Glu Gly Ala 420 425 430 Ile Ile Glu Asp Thr Leu Leu Met Gly Ala Asp Tyr Tyr Glu Thr Glu 435 440 445 Ala Asp Lys Lys Leu Leu Ala Asp Lys Gly Gly Ile Pro Ile Gly Ile 450 455 460 Gly Lys Asn Ser His Ile Arg Arg Ala Ile Ile Asp Lys Asn Ala Arg 465 470 475 480 Ile Gly Asp Asn Val Lys Ile Ile Asn Val Asp Asn Val Gln Glu Ala 485 490 495 Ala Arg Glu Thr Asp Gly Tyr Phe Ile Lys Ser Gly Ile Val Thr Val 500 505 510 Ile Lys Asp Ala Leu Leu Pro Ser Gly Thr Val Ile 515 520 76 398 PRT Festuca arundinacea 76 Met Ala Ala Thr Met Thr Val Glu Glu Val Arg Lys Ala Gln Arg Ala 1 5 10 15 Glu Gly Pro Ala Thr Val Leu Ala Ile Gly Thr Ala Thr Pro Ala Asn 20 25 30 Cys Val Tyr Gln Ala Asp Tyr Pro Asp Tyr Tyr Phe Lys Ile Thr Lys 35 40 45 Ser Asp His Leu Ala Asp Leu Lys Glu Lys Phe Lys Arg Met Cys Asp 50 55 60 Lys Ser Gln Ile Arg Lys Arg Tyr Met His Leu Thr Glu Glu Ile Leu 65 70 75 80 Glu Glu Asn Pro Asn Met Cys Ala Tyr Met Ala Pro Ser Leu Asp Ala 85 90 95 Arg Gln Asp Ile Val Val Val Glu Val Pro Lys Leu Gly Lys Ala Ala 100 105 110 Ala Gln Lys Ala Ile Lys Glu Trp Gly Gln Pro Arg Ser Lys Ile Thr 115 120 125 His Leu Val Phe Cys Thr Thr Ser Gly Val Asp Met Pro Gly Ala Asp 130 135 140 Tyr Gln Leu Thr Lys Met Leu Gly Leu Arg Pro Ser Val Lys Arg Leu 145 150 155 160 Met Met Tyr Gln Gln Gly Cys Phe Ala Gly Gly Thr Val Leu Arg Leu 165 170 175 Ala Lys Asp Leu Ala Glu Asn Asn Arg Gly Ala Arg Val Leu Val Val 180 185 190 Cys Ser Glu Ile Thr Ala Val Thr Phe Arg Gly Pro His Glu Ser His 195 200 205 Leu Asp Ser Leu Val Gly Gln Ala Leu Phe Gly Asp Gly Ala Ala Ala 210 215 220 Val Ile Ile Gly Ala Asp Pro Asp Val Ser Val Glu Arg Pro Leu Phe 225 230 235 240 Gln Leu Val Ser Val Ser Gln Thr Ile Leu Pro Asp Ser Glu Gly Ala 245 250 255 Ile Asp Gly His Leu Arg Glu Val Gly Leu Thr Phe His Leu Leu Lys 260 265 270 Asp Val Pro Gly Leu Ile Ser Lys Asn Ile Glu Arg Ala Leu Glu Glu 275 280 285 Ala Phe Lys Pro Leu Gly Ile Asp Asp Trp Asn Ser Val Phe Trp Val 290 295 300 Ala His Pro Gly Gly Pro Ala Ile Leu Asp Met Val Glu Ala Lys Val 305 310 315 320 Asn Leu Asn Lys Glu Arg Met Arg Ala Thr Arg His Val Leu Ser Glu 325 330 335 Tyr Gly Asn Met Ser Ser Ala Cys Val Leu Phe Ile Met Asp Glu Met 340 345 350 Arg Lys Arg Ser Ala Glu Asp Gly His Thr Thr Thr Gly Glu Gly Met 355 360 365 Asp Trp Gly Val Leu Phe Gly Phe Gly Pro Gly Leu Thr Val Glu Thr 370 375 380 Val Val Leu His Ser Met Pro Ile Ala Ala Asp Ala Thr Ala 385 390 395 77 398 PRT Festuca arundinacea 77 Met Ala Thr Thr Met Thr Val Glu Glu Val Arg Lys Ala Gln Arg Ala 1 5 10 15 Glu Gly Pro Ala Thr Val Leu Ala Ile Gly Thr Ala Thr Pro Ala Asn 20 25 30 Cys Val Tyr Gln Ala Asp Tyr Pro Asp Tyr Tyr Phe Lys Ile Thr Lys 35 40 45 Ser Asp His Leu Ala Asp Leu Lys Glu Lys Phe Lys Arg Met Cys Asp 50 55 60 Lys Ser Gln Ile Arg Lys Arg Tyr Met His Leu Thr Glu Glu Ile Leu 65 70 75 80 Glu Glu Asn Pro Asn Met Cys Ala Tyr Met Ala Pro Ser Leu Asp Ala 85 90 95 Arg Gln Asp Ile Val Val Val Glu Val Pro Lys Leu Gly Lys Ala Ala 100 105 110 Ala Gln Lys Ala Ile Lys Glu Trp Gly Gln Pro Arg Ser Lys Ile Thr 115 120 125 His Leu Val Phe Cys Thr Thr Ser Gly Val Asp Met Pro Gly Ala Asp 130 135 140 Tyr Gln Leu Thr Lys Met Leu Gly Leu Arg Pro Ser Val Lys Arg Leu 145 150 155 160 Met Met Tyr Gln Gln Gly Cys Phe Ala Gly Gly Thr Val Leu Arg Leu 165 170 175 Ala Lys Asp Leu Ala Glu Asn Asn Arg Gly Ala Arg Val Leu Val Val 180 185 190 Cys Ser Glu Ile Thr Ala Val Thr Phe Arg Gly Pro His Glu Ser His 195 200 205 Leu Asp Ser Leu Val Gly Gln Ala Leu Phe Gly Asp Gly Ala Ala Ala 210 215 220 Val Ile Ile Gly Ala Asp Pro Asp Val Ser Val Glu His Pro Leu Phe 225 230 235 240 Gln Leu Val Ser Ala Ser Gln Thr Ile Leu Pro Asp Ser Glu Gly Ala 245 250 255 Ile Asp Gly His Leu Arg Glu Val Gly Leu Thr Phe His Leu Leu Lys 260 265 270 Asp Val Pro Gly Leu Ile Ser Lys Asn Ile Glu Arg Ala Leu Glu Glu 275 280 285 Ala Phe Lys Pro Leu Gly Ile Asp Asp Trp Asn Ser Val Phe Trp Val 290 295 300 Ala His Pro Gly Gly Pro Ala Ile Leu Asp Met Val Glu Ala Lys Val 305 310 315 320 Asn Leu Asn Lys Glu Arg Met Arg Ala Thr Arg His Val Leu Ser Glu 325 330 335 Tyr Gly Asn Met Ser Ser Ala Cys Val Leu Phe Ile Met Asp Glu Met 340 345 350 Arg Lys Arg Ser Ala Glu Asp Gly His Thr Thr Thr Gly Glu Gly Met 355 360 365 Asp Trp Gly Val Leu Phe Gly Phe Gly Pro Gly Leu Thr Val Glu Thr 370 375 380 Val Val Leu His Ser Met Pro Ile Ala Ala Gly Ala Thr Ala 385 390 395 78 277 PRT Festuca arundinacea 78 Arg Ala Asp Leu Glu Glu Glu Gly Ser Phe Asp Asp Ala Val Ala Gly 1 5 10 15 Cys Asp Tyr Ala Phe Leu Val Ala Ala Pro Val Asn Leu Lys Ala Glu 20 25 30 Asn Pro Glu Lys Asp Met Val Glu Pro Ala Val Gly Gly Thr Leu Asn 35 40 45 Ala Met Arg Ser Cys Val Arg Ala Gly Thr Val Lys Arg Val Val Leu 50 55 60 Thr Ser Ser Val Ala Ser Val Ser Ala Arg Pro Leu Leu Gln Gly Asp 65 70 75 80 Gly His Val Leu Asp Glu Glu Ser Trp Ser Asp Val Asp Phe Leu Arg 85 90 95 Ala Lys Ala Thr Gly His Trp Gly Tyr Pro Val Ser Lys Val Leu Leu 100 105 110 Glu Lys Ala Ala Cys Ala Phe Ala Gln Ala Ser Gly Ile Ser Leu Val 115 120 125 Thr Val Cys Pro Val Val Val Val Gly Lys Ala Pro Ala Val Gln Val 130 135 140 His Thr Ser Val Pro Asp Val Leu Ser Pro Leu Ser Gly Asp Glu Ala 145 150 155 160 Lys Ile Gln Ile Leu Gln His Ile Glu Arg Ala Ser Gly Ser Ile Ser 165 170 175 Leu Val His Val Asp Asp Leu Cys Arg Ala Glu Val Phe Leu Ala Glu 180 185 190 Glu Glu Ala Val Ala Ser Gly Arg Tyr Ile Cys Cys Ser Leu Ser Thr 195 200 205 Thr Ala Gly Val Leu Ala Arg Phe Leu Ser Val Lys Tyr Pro Gln Tyr 210 215 220 Lys Val Arg Thr Asp Arg Phe Ser Gly Ser Pro Glu Lys Pro Arg Val 225 230 235 240 Cys Met Ser Ser Ala Lys Leu Val Ala Glu Gly Phe Gln Tyr Lys Tyr 245 250 255 Lys Thr Leu Asp Glu Ile Tyr Asp Asp Val Val Glu Tyr Gly Arg Ala 260 265 270 Leu Gly Ile Leu Pro 275 79 342 PRT Festuca arundinacea 79 Met Ala Ala Ala Gly Asp Gly Ser Arg Arg Lys Thr Ala Cys Val Thr 1 5 10 15 Gly Gly Asn Gly Tyr Ile Ala Ser Ala Leu Val Lys Met Leu Leu Glu 20 25 30 Lys Gly Tyr Ala Val Lys Thr Thr Val Arg Asn Pro Asp Asp Met Glu 35 40 45 Lys Asn Ser His Leu Lys Asp Leu Gln Ala Leu Gly Pro Leu Glu Val 50 55 60 Phe Arg Ala Asp Leu Gln Glu Glu Gly Ser Phe Asp Asp Ala Val Ala 65 70 75 80 Gly Cys Asp Tyr Ala Phe Leu Val Ala Ala Pro Val Asn Leu Lys Ala 85 90 95 Glu Asn Pro Glu Lys Asp Met Val Glu Pro Ala Val Gly Gly Thr Leu 100 105 110 Asn Ala Met Arg Ser Cys Val Arg Ala Gly Thr Val Lys Arg Val Val 115 120 125 Leu Thr Ser Ser Val Ala Ser Val Ser Ala Arg Pro Leu Leu Gln Gly 130 135 140 Asp Gly His Val Leu Asp Glu Glu Ser Trp Ser Asp Val Asp Phe Leu 145 150 155 160 Arg Ala Lys Ala Thr Gly His Trp Gly Tyr Pro Val Ser Lys Val Leu 165 170 175 Leu Glu Lys Ala Ala Cys Ala Phe Ala Gln Ala Ser Gly Ile Ser Leu 180 185 190 Val Thr Val Cys Pro Val Val Val Val Gly Lys Ala Pro Ala Val Gln 195 200 205 Val His Thr Ser Val Pro Asp Val Leu Ser Pro Leu Ser Gly Asp Glu 210 215 220 Ala Lys Ile Gln Ile Leu Gln His Ile Glu Arg Ala Ser Gly Ser Ile 225 230 235 240 Ser Leu Val His Val Asp Asp Leu Cys Arg Ala Glu Val Phe Leu Ala 245 250 255 Glu Glu Glu Ala Val Ala Ser Gly Arg Tyr Ile Cys Cys Ser Leu Ser 260 265 270 Thr Thr Ala Gly Val Leu Ala Arg Phe Leu Ser Val Lys Tyr Pro Gln 275 280 285 Tyr Lys Val Arg Thr Asp Arg Phe Ser Gly Ser Pro Glu Lys Pro Arg 290 295 300 Val Cys Met Ser Ser Ala Lys Leu Val Ala Glu Gly Phe Gln Tyr Lys 305 310 315 320 Tyr Lys Thr Leu Asp Glu Ile Tyr Asp Asp Val Val Glu Tyr Gly Arg 325 330 335 Ala Leu Gly Ile Leu Pro 340 80 255 PRT Lolium perenne 80 Phe Ile Ser Val Thr Val Phe Tyr Val Val Gly Leu Arg Gln Arg Asp 1 5 10 15 Leu Val Gln Ala Gly Val Gln Gly Thr Leu Asn Val Met Arg Ser Cys 20 25 30 Val Lys Ala Gly Thr Val Lys Arg Val Ile Leu Thr Ser Ser Asp Ser 35 40 45 Ala Val Cys Gln Arg Pro Leu Glu Gly Asp Gly His Val Leu Asp Glu 50 55 60 Gly Ser Trp Ser Asp Val Pro Tyr Leu Arg Ala Glu Gln Pro Glu Ala 65 70 75 80 Trp Gly Tyr Ala Val Ser Lys Val Leu Met Glu Glu Ala Ala Gly Lys 85 90 95 Phe Ala Asp Glu Asn Gly Leu Gly Leu Val Ser Val Leu Pro Thr Phe 100 105 110 Thr Leu Gly Ala Ala Pro Val Ser Gln Ala Arg Thr Ser Val Pro Val 115 120 125 Val Leu Ser Leu Leu Ser Gly Asp Glu Glu Gln Leu Asn Leu Leu Glu 130 135 140 Ala Met His Leu Ile Thr Glu Ser Val Ser Ile Asn His Ile Asp Asp 145 150 155 160 Leu Cys Arg Ala Gln Val Phe Leu Ala Glu Asn Glu Ala Ser Ser Gly 165 170 175 Arg Tyr Ile Cys Ser Ser His Asp Thr Thr Val Val Gln Leu Ala Arg 180 185 190 Leu Leu Ala Asp Lys Tyr Pro Gln Tyr Asn Val Lys Ser Gln Arg Phe 195 200 205 Asp Gly Ser Pro Glu Lys Pro Arg Val Cys Leu Ser Ser Gln Lys Leu 210 215 220 Ile Gly Glu Gly Phe Val Tyr Lys Tyr Asp Asp Leu Gly Ala Ile Leu 225 230 235 240 Asp Asp Leu Val Glu Tyr Gly Arg Thr Thr Gly Ile Leu Pro Phe 245 250 255 81 340 PRT Lolium perenne 81 Met Ala Ser Ala Ala Gly Gly Arg Arg Lys Thr Ala Cys Val Thr Gly 1 5 10 15 Gly Ser Gly Tyr Ile Ala Ser Ala Leu Ile Lys Thr Leu Leu Asp His 20 25 30 Gly Tyr Ala Val Lys Thr Thr Val Arg Asn Pro Asp Asp Leu Glu Lys 35 40 45 Thr Ser His Leu Lys Asp Leu Gln Ala Phe Gly Pro Leu Glu Ile Phe 50 55 60 Arg Gly Glu Leu Asp Val Glu Gly Ser Phe Asp Asp Ser Val Ser Gly 65 70 75 80 Cys Asp Tyr Val Phe Leu Val Ala Ala Pro Met Asp Met Gly Ser Leu 85 90 95 Asn Pro Glu Arg Asp Leu Val Gln Ala Gly Val Gln Gly Thr Leu Asn 100 105 110 Val Met Arg Ser Cys Val Lys Ala Gly Thr Val Lys Arg Val Ile Leu 115 120 125 Thr Ser Ser Asp Ser Ala Val Cys Gln Arg Pro Leu Glu Gly Asp Gly 130 135 140 His Val Leu Asp Glu Gly Ser Trp Ser Asp Val Pro Tyr Leu Arg Ala 145 150 155 160 Glu Gln Pro Glu Ala Trp Gly Tyr Ala Val Ser Lys Val Leu Met Glu 165 170 175 Glu Ala Ala Gly Lys Phe Ala Asp Glu Asn Gly Leu Gly Leu Val Ser 180 185 190 Val Leu Pro Thr Phe Thr Leu Gly Ala Ala Pro Val Ser Gln Ala Arg 195 200 205 Thr Ser Val Pro Val Val Leu Ser Leu Leu Ser Gly Asp Glu Glu Gln 210 215 220 Leu Asn Leu Leu Glu Ala Met His Leu Ile Thr Glu Ser Val Ser Ile 225 230 235 240 Asn His Ile Asp Asp Leu Cys Arg Ala Gln Val Phe Leu Ala Glu Asn 245 250 255 Glu Ala Ser Ser Gly Arg Tyr Ile Cys Ser Ser His Asp Thr Thr Val 260 265 270 Val Gln Leu Ala Arg Leu Leu Ala Asp Lys Tyr Pro Gln Tyr Asn Val 275 280 285 Lys Ser Gln Arg Phe Asp Gly Ser Pro Glu Lys Pro Arg Val Cys Leu 290 295 300 Ser Ser Gln Lys Leu Ile Gly Glu Gly Phe Val Tyr Lys Tyr Asp Asp 305 310 315 320 Leu Gly Ala Ile Leu Asp Asp Leu Val Glu Tyr Gly Arg Thr Thr Gly 325 330 335 Ile Leu Pro Phe 340 82 508 PRT Lolium perenne 82 Ala Ala Ala Ser Ile Trp Phe Leu Phe Arg Gly Ser Ser Ser Gly Lys 1 5 10 15 Lys Leu Ser Lys Leu Pro Leu Pro Pro Gly Pro Arg Gly Trp Pro Val 20 25 30 Leu Gly Asn Leu Pro Gln Val Gly Ala Lys Pro His His Thr Met Ala 35 40 45 Ala Leu Ser Gln Gln Phe Gly Pro Leu Phe Arg Leu Arg Phe Gly Val 50 55 60 Ala Glu Val Val Val Ala Ala Ser Ala Lys Val Ala Ser Gln Phe Leu 65 70 75 80 Arg Ala His Asp Ala Asn Phe Ser Asp Arg Pro Pro Asn Ser Gly Ala 85 90 95 Glu His Val Ala Tyr Asn Tyr Gln Asp Leu Val Phe Ala Pro Tyr Gly 100 105 110 Ser Arg Trp Arg Ala Leu Arg Lys Leu Cys Ala Leu His Leu Phe Ser 115 120 125 Ala Lys Ala Leu Asp Ala Leu Arg Ala Val Arg Glu Ala Glu Val Ala 130 135 140 Leu Met Val Lys Gln Leu Lys Glu Ser Ala Pro Ala Gly Val Val Val 145 150 155 160 Gly Gln Glu Ala Asn Val Cys Ala Thr Asn Ala Leu Ala Arg Ala Ala 165 170 175 Val Gly Arg Arg Val Phe Gly Gly Ser Ala Gly Glu Gly Ala Arg Glu 180 185 190 Phe Lys Asp Met Val Val Glu Leu Met Gln Leu Ala Gly Val Phe Asn 195 200 205 Ile Gly Asp Phe Val Pro Ala Leu Arg Trp Leu Asp Pro Gln Gly Val 210 215 220 Val Ala Arg Met Lys Arg Leu His Arg Arg Tyr Asp Ala Met Met Asp 225 230 235 240 Gly Phe Ile Ser Glu Arg Asp Gln Arg His Asn Gln Ala Ala Ala Asp 245 250 255 Gly Glu Arg Lys Asp Leu Leu Ser Val Met Leu Gly Tyr Met Arg Pro 260 265 270 Asp Gly Gly Gly Gly Glu Glu Glu Gly Ile Ser Phe Asn His Thr Asp 275 280 285 Ile Lys Ala Leu Leu Leu Asn Leu Phe Thr Ala Gly Thr Asp Thr Thr 290 295 300 Ser Ser Thr Val Glu Trp Ala Leu Ala Glu Leu Ile Arg His Lys Asp 305 310 315 320 Val Leu Thr Gln Ala Gln Arg Glu Leu Asp Asp Ile Val Gly Gln Asp 325 330 335 Arg Leu Val Thr Glu Ser Asp Leu Pro His Leu Thr Phe Leu Thr Ala 340 345 350 Val Ile Lys Glu Thr Phe Arg Leu His Pro Ser Thr Pro Leu Ser Leu 355 360 365 Pro Arg Val Ala Thr Glu Asp Cys Glu Val Glu Gly Tyr Arg Ile Pro 370 375 380 Lys Gly Thr Thr Leu Leu Val Asn Val Trp Ala Ile Ala Arg Asp Pro 385 390 395 400 Ala Ser Trp Gly Pro Asp Ala Leu Glu Phe Arg Pro Ala Arg Phe Leu 405 410 415 Ala Gly Gly Leu His Glu Ser Val Asp Val Lys Gly Ser Asp Tyr Glu 420 425 430 Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Leu Ser Trp 435 440 445 Gly Leu Arg Met Val Thr Leu Met Thr Ala Thr Leu Val His Ala Phe 450 455 460 Asp Trp Ser Leu Val Asp Gly Leu Thr Pro Glu Lys Leu Asp Met Glu 465 470 475 480 Glu Ala Tyr Gly Leu Thr Leu Gln Arg Ala Ala Pro Leu Met Val Arg 485 490 495 Pro Ile Pro Arg Leu Leu Ser Ser Ala Tyr Thr Val 500 505 83 524 PRT Lolium perenne 83 Met Asp His Arg Asp Val Leu Val Leu Leu Cys Ser Leu Ala Ala Leu 1 5 10 15 Ala Ala Ala Ser Ile Trp Phe Leu Phe Arg Gly Ser Ser Ser Gly Lys 20 25 30 Lys Leu Ser Lys Leu Pro Leu Pro Pro Gly Pro Arg Gly Trp Pro Val 35 40 45 Leu Gly Asn Leu Pro Gln Val Gly Ala Lys Pro His His Thr Met Ala 50 55 60 Ala Leu Ser Gln Gln Phe Gly Pro Leu Phe Arg Leu Arg Phe Gly Val 65 70 75 80 Ala Glu Val Val Val Ala Ala Ser Ala Lys Val Ala Ser Gln Phe Leu 85 90 95 Arg Ala His Asp Ala Asn Phe Ser Asp Arg Pro Pro Asn Ser Gly Ala 100 105 110 Glu His Val Ala Tyr Asn Tyr Gln Asp Leu Val Phe Ala Pro Tyr Gly 115 120 125 Ser Arg Trp Arg Ala Leu Arg Lys Leu Cys Ala Leu His Leu Phe Ser 130 135 140 Ala Lys Ala Leu Asp Ala Leu Arg Ala Val Arg Glu Ala Glu Val Ala 145 150 155 160 Leu Met Val Lys Gln Leu Lys Glu Ser Ala Pro Ala Gly Val Val Val 165 170 175 Gly Gln Glu Ala Asn Val Cys Ala Thr Asn Ala Leu Ala Arg Ala Ala 180 185 190 Val Gly Arg Arg Val Phe Gly Gly Ser Ala Gly Glu Gly Ala Arg Glu 195 200 205 Phe Lys Asp Met Val Val Glu Leu Met Gln Leu Ala Gly Val Phe Asn 210 215 220 Ile Gly Asp Phe Val Pro Ala Leu Arg Trp Leu Asp Pro Gln Gly Val 225 230 235 240 Val Ala Arg Met Lys Arg Leu His Arg Arg Tyr Asp Ala Met Met Asp 245 250 255 Gly Phe Ile Ser Glu Arg Asp Gln Arg His Asn Gln Ala Ala Ala Asp 260 265 270 Gly Glu Arg Lys Asp Leu Leu Ser Val Met Leu Gly Tyr Met Arg Pro 275 280 285 Asp Gly Gly Gly Gly Glu Glu Glu Gly Ile Ser Phe Asn His Thr Asp 290 295 300 Ile Lys Ala Leu Leu Leu Asn Leu Phe Thr Ala Gly Thr Asp Thr Thr 305 310 315 320 Ser Ser Thr Val Glu Trp Ala Leu Ala Glu Leu Ile Arg His Lys Asp 325 330 335 Val Leu Thr Gln Ala Gln Arg Glu Leu Asp Asp Ile Val Gly Gln Asp 340 345 350 Arg Leu Val Thr Glu Ser Asp Leu Pro His Leu Thr Phe Leu Thr Ala 355 360 365 Val Ile Lys Glu Thr Phe Arg Leu His Pro Ser Thr Pro Leu Ser Leu 370 375 380 Pro Arg Val Ala Thr Glu Asp Cys Glu Val Glu Gly Tyr Arg Ile Pro 385 390 395 400 Lys Gly Thr Thr Leu Leu Val Asn Val Trp Ala Ile Ala Arg Asp Pro 405 410 415 Ala Ser Trp Gly Pro Asp Ala Leu Glu Phe Arg Pro Ala Arg Phe Leu 420 425 430 Ala Gly Gly Leu His Glu Ser Val Asp Val Lys Gly Ser Asp Tyr Glu 435 440 445 Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Leu Ser Trp 450 455 460 Gly Leu Arg Met Val Thr Leu Met Thr Ala Thr Leu Val His Ala Phe 465 470 475 480 Asp Trp Ser Leu Val Asp Gly Leu Thr Pro Glu Lys Leu Asp Met Glu 485 490 495 Glu Ala Tyr Gly Leu Thr Leu Gln Arg Ala Ala Pro Leu Met Val Arg 500 505 510 Pro Ile Pro Arg Leu Leu Ser Ser Ala Tyr Thr Val 515 520 84 525 PRT Festuca arundinacea 84 Arg Ser Glu Leu Ala Gly Met Asp Ile Pro Leu Ser Leu Leu Leu Ser 1 5 10 15 Thr Leu Ala Ile Ser Ala Thr Ile Cys Tyr Val Phe Phe Arg Ala Gly 20 25 30 Lys Gly His Arg Ala Pro Leu Pro Leu Pro Pro Gly Pro Arg Gly Trp 35 40 45 Pro Val Leu Gly Asn Leu Pro Gln Leu Gly Gly Lys Thr His Gln Thr 50 55 60 Leu His Glu Met Thr Lys Val Tyr Gly Pro Val Leu Arg Leu Arg Phe 65 70 75 80 Gly Ser Ser Val Val Val Val Ala Gly Ser Ala Ala Val Ala Glu Gln 85 90 95 Phe Leu Arg Thr His Asp Ala Lys Phe Ser Ser Arg Pro Pro Asn Ser 100 105 110 Gly Gly Glu His Met Ala Tyr Asn Tyr Arg Asp Val Val Phe Ala Pro 115 120 125 Tyr Gly Pro Arg Trp Arg Ala Met Arg Lys Val Cys Ala Val Asn Ile 130 135 140 Phe Ser Ala Arg Ala Leu Asp Asp Leu Arg Gly Phe Arg Glu Arg Glu 145 150 155 160 Ala Ala Leu Met Val Arg Ser Leu Ala Asp Ala Ala Lys Ala Gly Val 165 170 175 Ala Val Ala Val Gly Lys Ala Ala Asn Val Cys Thr Thr Asn Gly Leu 180 185 190 Ser Arg Ala Ala Val Gly Leu Arg Val Phe Gly Ser Asp Gly Ala Arg 195 200 205 Asp Phe Lys Glu Ile Val Leu Glu Val Met Glu Val Gly Gly Val Leu 210 215 220 Asn Val Gly Asp Phe Val Pro Ala Leu Arg Trp Leu Asp Pro Gln Gly 225 230 235 240 Val Val Ala Arg Leu Lys Lys Leu His Arg Arg Phe Asp Asp Met Met 245 250 255 Asn Gly Ile Ile Ala Glu Arg Arg Thr Gly Thr Lys Thr Ala Val Val 260 265 270 Glu Glu Gly Lys Gly Asp Leu Leu Gly Leu Leu Leu Ala Met Val Gln 275 280 285 Glu Asp Lys Ser Leu Thr Gly Ser Glu Glu Asp Lys Ile Thr Asp Thr 290 295 300 Asp Val Lys Ala Leu Ile Leu Asn Leu Phe Val Ala Gly Thr Glu Thr 305 310 315 320 Thr Ser Ser Ile Val Glu Trp Ala Val Ala Glu Leu Ile Arg His Pro 325 330 335 Asp Ile Leu Lys Gln Ala Gln Glu Glu Leu Asp Ala Val Val Gly Arg 340 345 350 Asp Arg Leu Val Ser Glu Ser Asp Leu Pro Arg Leu Thr Phe Phe Asn 355 360 365 Ala Ile Ile Lys Glu Thr Phe Arg Leu His Pro Ser Thr Pro Leu Ser 370 375 380 Leu Pro Arg Met Ala Ser Glu Glu Cys Glu Val Ala Gly Tyr His Ile 385 390 395 400 Pro Arg Gly Thr Glu Leu Leu Val Asn Val Trp Gly Ile Ala Arg Asp 405 410 415 Pro Ala Leu Trp Pro Asp Pro Leu Glu Tyr Gln Pro Ala Arg Phe Leu 420 425 430 Pro Gly Gly Ser His Glu Asn Val Asp Leu Lys Gly Gly Asp Phe Gly 435 440 445 Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Leu Ser Trp 450 455 460 Gly Leu Arg Met Val Thr Ile Thr Thr Ala Thr Leu Val His Ser Phe 465 470 475 480 Asp Trp Glu Leu Pro Ala Gly Gln Thr Pro Asp Lys Leu Asn Met Glu 485 490 495 Glu Ala Phe Ser Leu Leu Leu Gln Arg Ala Val Pro Leu Met Val His 500 505 510 Pro Val Pro Arg Leu Leu Pro Ser Ala Tyr Glu Ile Ser 515 520 525 85 526 PRT Festuca arundinacea 85 Met Arg Ser Glu Leu Ala Gly Met Asp Ile Pro Leu Pro Leu Leu Leu 1 5 10 15 Ser Thr Leu Ala Ile Ser Ala Thr Ile Cys Tyr Val Phe Phe Arg Ala 20 25 30 Gly Lys Gly His Arg Ala Pro Leu Pro Leu Pro Pro Gly Pro Arg Gly 35 40 45 Trp Pro Val Leu Gly Asn Leu Pro Gln Leu Gly Gly Lys Thr His Gln 50 55 60 Thr Leu His Glu Met Thr Lys Val Tyr Gly Pro Val Leu Arg Leu Arg 65 70 75 80 Phe Gly Ser Ser Val Val Val Val Ala Gly Ser Ala Ala Val Ala Glu 85 90 95 Gln Phe Leu Arg Thr His Asp Ala Lys Phe Ser Ser Arg Pro Pro Asn 100 105 110 Ser Gly Gly Glu His Met Ala Tyr Asn Tyr Arg Asp Val Val Phe Ala 115 120 125 Pro Tyr Gly Pro Arg Trp Arg Ala Met Arg Lys Val Cys Ala Val Asn 130 135 140 Ile Phe Ser Ala Arg Ala Leu Asp Asp Leu Arg Gly Phe Arg Glu Arg 145 150 155 160 Glu Ala Ala Leu Met Val Arg Ser Leu Ala Asp Ala Ala Lys Ala Gly 165 170 175 Val Ala Val Ala Val Gly Lys Ala Ala Asn Val Cys Thr Thr Asn Gly 180 185 190 Leu Ser Arg Ala Ala Val Gly Leu Arg Val Phe Gly Ser Asp Gly Ala 195 200 205 Arg Asp Phe Lys Glu Ile Val Leu Glu Val Met Glu Val Gly Gly Val 210 215 220 Leu Asn Val Gly Asp Phe Val Pro Ala Leu Arg Trp Leu Asp Pro Gln 225 230 235 240 Gly Val Val Ala Arg Leu Lys Lys Leu His Arg Arg Phe Asp Asp Met 245 250 255 Met Asn Gly Ile Ile Ala Glu Arg Arg Thr Gly Thr Lys Thr Ala Val 260 265 270 Val Glu Glu Gly Lys Gly Asp Leu Leu Gly Leu Leu Leu Ala Met Val 275 280 285 Gln Glu Asp Lys Ser Leu Thr Gly Ser Glu Glu Asp Lys Ile Thr Asp 290 295 300 Thr Asp Val Lys Ala Leu Ile Leu Asn Leu Phe Val Ala Gly Thr Glu 305 310 315 320 Thr Thr Ser Ser Ile Val Glu Trp Ala Val Ala Glu Leu Ile Arg His 325 330 335 Pro Asp Ile Leu Lys Gln Ala Gln Glu Glu Leu Asp Ala Val Val Gly 340 345 350 Arg Asp Arg Leu Val Ser Glu Ser Asp Leu Pro Arg Leu Thr Phe Phe 355 360 365 Asn Ala Ile Ile Lys Glu Thr Phe Arg Leu His Pro Ser Thr Pro Leu 370 375 380 Ser Leu Pro Arg Met Ala Ser Glu Glu Cys Glu Val Ala Gly Tyr His 385 390 395 400 Ile Pro Arg Gly Thr Glu Leu Leu Val Asn Val Trp Gly Ile Ala Arg 405 410 415 Asp Pro Ala Leu Trp Pro Asp Pro Leu Glu Tyr Gln Pro Ala Arg Phe 420 425 430 Leu Pro Gly Gly Ser His Glu Asn Val Asp Leu Lys Gly Gly Asp Phe 435 440 445 Gly Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Leu Ser 450 455 460 Trp Gly Leu Arg Met Val Thr Ile Thr Thr Ala Thr Leu Val His Ser 465 470 475 480 Phe Asp Trp Glu Leu Pro Ala Gly Gln Thr Pro Asp Lys Leu Asn Met 485 490 495 Glu Glu Ala Phe Ser Leu Leu Leu Gln Arg Ala Val Pro Leu Met Val 500 505 510 His Pro Val Pro Arg Leu Leu Pro Ser Ala Tyr Glu Ile Ser 515 520 525 86 491 PRT Festuca arundinacea 86 Asp Ile Pro Leu Pro Leu Leu Leu Ser Thr Leu Ala Ile Ser Ala Thr 1 5 10 15 Ile Cys Tyr Val Phe Phe Arg Ala Gly Lys Thr His Gln Thr Leu His 20 25 30 Glu Met Thr Lys Val Tyr Gly Pro Val Leu Arg Leu Arg Phe Gly Ser 35 40 45 Ser Val Val Val Val Ala Gly Ser Ala Ala Val Ala Glu Gln Phe Leu 50 55 60 Arg Thr His Asp Ala Lys Phe Ser Ser Arg Pro Pro Asn Ser Gly Gly 65 70 75 80 Glu His Met Ala Tyr Asn Tyr Gln Asp Ile Val Phe Ala Pro Tyr Gly 85 90 95 Pro Arg Trp Arg Ala Met Arg Lys Val Cys Ala Val Asn Ile Phe Ser 100 105 110 Ala Arg Ala Leu Asp Asp Leu Arg Gly Phe Arg Glu Arg Glu Ala Ala 115 120 125 Leu Met Val Arg Ser Leu Ala Asp Ala Ala Lys Ala Gly Ala Ala Val 130 135 140 Ala Val Gly Lys Ala Ala Asn Val Cys Thr Thr Asn Gly Leu Ser Arg 145 150 155 160 Ala Ala Val Gly Leu Arg Val Phe Gly Ser Asp Gly Thr Arg Asp Phe 165 170 175 Lys Glu Ile Val Leu Glu Val Met Glu Val Gly Gly Val Leu Asn Val 180 185 190 Gly Asp Phe Val Pro Ala Leu Arg Trp Leu Asp Pro Gln Gly Val Val 195 200 205 Ala Arg Met Lys Lys Leu His Arg Arg Phe Asp Asp Ile Met Asn Gly 210 215 220 Ile Ile Ala Glu Arg Arg Thr Gly Ala Lys Thr Ala Val Val Glu Glu 225 230 235 240 Gly Lys Gly Asp Leu Leu Gly Leu Leu Leu Ala Met Val Gln Glu Asp 245 250 255 Lys Ser Leu Thr Gly Ser Glu Glu Asp Lys Ile Thr Asp Thr Asp Val 260 265 270 Lys Ala Leu Ile Leu Asn Leu Phe Val Ala Gly Thr Glu Thr Thr Ser 275 280 285 Ser Ile Val Glu Trp Ala Val Ala Glu Leu Ile Arg His Pro Asp Ile 290 295 300 Leu Lys Gln Ala Gln Glu Glu Leu Asp Thr Val Val Gly Arg Asp Arg 305 310 315 320 Ile Val Ser Glu Ser Asp Leu Pro Arg Leu Thr Phe Phe Asn Ala Ile 325 330 335 Ile Lys Glu Thr Phe Arg Leu His Pro Ser Thr Pro Leu Ser Leu Pro 340 345 350 Arg Met Ala Ser Glu Asp Cys Glu Val Ala Gly Tyr His Ile Pro Arg 355 360 365 Gly Thr Glu Leu Leu Val Asn Val Trp Gly Ile Ala Arg Asp Pro Ser 370 375 380 Leu Trp Pro Asp Pro Leu Glu Tyr Arg Pro Ala Arg Phe Leu Pro Gly 385 390 395 400 Gly Ser His Glu Asn Val Asp Leu Lys Gly Gly Asp Phe Gly Leu Ile 405 410 415 Pro Phe Gly Ala Gly Arg Arg Ile Cys Ala Gly Leu Ser Trp Gly Leu 420 425 430 Arg Met Val Thr Val Thr Thr Ala Thr Leu Val His Ser Phe Asp Trp 435 440 445 Glu Leu Pro Ala Gly Gln Thr Leu Asp Lys Leu Asn Met Glu Glu Ala 450 455 460 Phe Ser Leu Leu Leu Gln Arg Ala Met Pro Leu Met Val His Pro Val 465 470 475 480 Pro Arg Leu Leu Pro Ser Ala Tyr Glu Ile Ser 485 490 87 499 PRT Festuca arundinacea 87 Met Arg Asn Glu Leu Ala Gly Met Asp Ile Pro Leu Pro Leu Leu Leu 1 5 10 15 Ser Thr Leu Ala Ile Ser Ala Thr Ile Cys Tyr Val Phe Phe Arg Ala 20 25 30 Gly Lys Thr His Gln Thr Leu His Glu Met Thr Lys Val Tyr Gly Pro 35 40 45 Val Leu Arg Leu Arg Phe Gly Ser Ser Val Val Val Val Ala Gly Ser 50 55 60 Ala Ala Val Ala Glu Gln Phe Leu Arg Thr His Asp Ala Lys Phe Ser 65 70 75 80 Ser Arg Pro Pro Asn Ser Gly Gly Glu His Met Ala Tyr Asn Tyr Gln 85 90 95 Asp Ile Val Phe Ala Pro Tyr Gly Pro Arg Trp Arg Ala Met Arg Lys 100 105 110 Val Cys Ala Val Asn Ile Phe Ser Ala Arg Ala Leu Asp Asp Leu Arg 115 120 125 Gly Phe Arg Glu Arg Glu Ala Ala Leu Met Val Arg Ser Leu Ala Asp 130 135 140 Ala Ala Lys Ala Gly Ala Ala Val Ala Val Gly Lys Ala Ala Asn Val 145 150 155 160 Cys Thr Thr Asn Gly Leu Ser Arg Ala Ala Val Gly Leu Arg Val Phe 165 170 175 Gly Ser Asp Gly Thr Arg Asp Phe Lys Glu Ile Val Leu Glu Val Met 180 185 190 Glu Val Gly Gly Val Leu Asn Val Gly Asp Phe Val Pro Ala Leu Arg 195 200 205 Trp Leu Asp Pro Gln Gly Val Val Ala Arg Met Lys Lys Leu His Arg 210 215 220 Arg Phe Asp Asp Ile Met Asn Gly Ile Ile Ala Glu Arg Arg Thr Gly 225 230 235 240 Ala Lys Thr Ala Val Val Glu Glu Gly Lys Gly Asp Leu Leu Gly Leu 245 250 255 Leu Leu Ala Met Val Gln Glu Asp Lys Ser Leu Thr Gly Ser Glu Glu 260 265 270 Asp Lys Ile Thr Asp Thr Asp Val Lys Ala Leu Ile Leu Asn Leu Phe 275 280 285 Val Ala Gly Thr Glu Thr Thr Ser Ser Ile Val Glu Trp Ala Val Ala 290 295 300 Glu Leu Ile Arg His Pro Asp Ile Leu Lys Gln Ala Gln Glu Glu Leu 305 310 315 320 Asp Thr Val Val Gly Arg Asp Arg Ile Val Ser Glu Ser Asp Leu Pro 325 330 335 Arg Leu Thr Phe Phe Asn Ala Ile Ile Lys Glu Thr Phe Arg Leu His 340 345 350 Pro Ser Thr Pro Leu Ser Leu Pro Arg Met Ala Ser Glu Asp Cys Glu 355 360 365 Val Ala Gly Tyr His Ile Pro Arg Gly Thr Glu Leu Leu Val Asn Val 370 375 380 Trp Gly Ile Ala Arg Asp Pro Ser Leu Trp Pro Asp Pro Leu Glu Tyr 385 390 395 400 Arg Pro Ala Arg Phe Leu Pro Gly Gly Ser His Glu Asn Val Asp Leu 405 410 415 Lys Gly Gly Asp Phe Gly Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 420 425 430 Cys Ala Gly Leu Ser Trp Gly Leu Arg Met Val Thr Val Thr Thr Ala 435 440 445 Thr Leu Val His Ser Phe Asp Trp Glu Leu Pro Ala Gly Gln Thr Leu 450 455 460 Asp Lys Leu Asn Met Glu Glu Ala Phe Ser Leu Leu Leu Gln Arg Ala 465 470 475 480 Met Pro Leu Met Val His Pro Val Pro Arg Leu Leu Pro Ser Ala Tyr 485 490 495 Glu Ile Ser 88 380 PRT Lolium perenne 88 Met Ala Met Ala Asp Cys Met Gln Glu Trp Pro Glu Pro Val Val Arg 1 5 10 15 Val Gln Ala Val Ala Glu Ser Gly Leu Ala Ala Ile Pro Asp Cys Tyr 20 25 30 Val Lys Pro Pro Arg Asp Arg Pro Ala Ala Gln His Leu Ala Thr Ala 35 40 45 Ala Ser Ala Asp Gly Asp Val Leu His Glu Pro Leu Asp Thr Ser Ile 50 55 60 Pro Val Ile Asp Leu Gly Glu Leu Val Ala Ala Thr Ala Asp Glu Gly 65 70 75 80 Arg Met Arg Gln Ile Met Glu Ala Val Ala Ala Ala Cys Arg Glu Trp 85 90 95 Gly Phe Phe Gln Val Val Asn His Gly Val Ala Pro Glu Leu Met His 100 105 110 Ala Ala Arg Glu Ala Trp Arg Gly Phe Phe Arg Leu Pro Ile Thr Ala 115 120 125 Lys Gln Gln Tyr Ala Asn Leu Pro Arg Thr Tyr Glu Gly Tyr Gly Ser 130 135 140 Arg Val Gly Val Gln Lys Gly Gly Pro Leu Asp Trp Gly Asp Tyr Tyr 145 150 155 160 Phe Leu His Leu Ala Pro Asp Ala Gly Lys Ser Pro Asp Lys Tyr Trp 165 170 175 Pro Thr Asn Pro Ala Ile Cys Lys Asp Val Ser Glu Glu Tyr Gly Arg 180 185 190 Glu Val Ile Arg Leu Cys Glu Leu Leu Met Lys Val Met Ser Ala Ser 195 200 205 Leu Gly Leu Glu Ala Thr Arg Phe Gln Glu Ala Phe Gly Gly Ser Glu 210 215 220 Cys Gly Val Cys Leu Arg Ala Asn Tyr Tyr Pro Arg Cys Pro Gln Pro 225 230 235 240 Asp Leu Thr Leu Gly Leu Ser Ala His Ser Asp Pro Gly Val Leu Thr 245 250 255 Val Leu Leu Ala Asp Glu His Val Arg Gly Leu Gln Val Arg Arg Ala 260 265 270 Asp Gly Glu Trp Val Thr Val Gln Pro Ala Arg His Asp Ala Phe Ile 275 280 285 Val Asn Val Gly Asp Gln Ile Gln Ile Leu Ser Asn Ser Met Tyr Lys 290 295 300 Ser Val Glu His Arg Val Met Val Asn Ala Lys Glu Glu Arg Ile Ser 305 310 315 320 Leu Ala Leu Phe Tyr Asn Pro Arg Gly Asp Val Pro Ile Ala Pro Ala 325 330 335 Pro Glu Thr Val Thr Pro Glu Arg Pro Ala Leu Tyr Pro Ser Met Thr 340 345 350 Phe Asp Glu Tyr Arg Ala Tyr Ile Arg Lys Tyr Gly Pro Arg Gly Lys 355 360 365 Ala Gln Val Glu Gly Ala Lys Gln Gly Gln Gly Ser 370 375 380 89 20 DNA Artificial Sequence Made in the lab 89 gacgcaagga gagatccaga 20 90 20 DNA Artificial Sequence Made in the lab 90 agacgaggtg ggtgatcttg 20 91 26 DNA Artificial Sequence Made in the lab 91 tacatatgaa gagagtttca tcgcat 26 92 21 DNA Artificial Sequence Made in the lab 92 gccgaacaga ccattgaagt a 21 

We claim:
 1. An isolated polynucleotide comprising a sequence selected from the group consisting of: SEQ ID NO: 1-44.
 2. An isolated polynucleotide comprising a sequence selected from the group consisting of: (a) complements of SEQ ID NO: 1-44; (b) reverse complements of SEQ ID NO: 1-44; (c) reverse sequences of SEQ ID NO: 1-44; (d) sequences that are 100-mers of a sequence of SEQ ID NO: 1-44; (e) sequences that are 40-mers of a sequence of SEQ ID NO: 1-44; and (f) sequences that are 20-mers of a sequence of SEQ ID NO: 1-44.
 3. An isolated polynucleotide comprising a sequence selected from the group consisting of: (a) sequences having at least 75% identity to a sequence of SEQ ID NO: 1-44; (b) sequences having at least 90% identity to a sequence of SEQ ID NO: 1-44; (c) sequences having at least 95% identity to a sequence of SEQ ID NO: 1-44; (d) sequences having at least 98% identity to a sequence of SEQ ID NO: 1-44; and (e) sequences that hybridize to a sequence of SEQ ID NO: 1-44 under stringent hybridization conditions, wherein the polynucleotide encodes a polypeptide having substantially the same functional activity as a polypeptide encoded by a polynucleotide of SEQ ID NO: 1-44.
 4. An isolated polypeptide encoded by a polynucleotide of any one of claims 1-3.
 5. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: sequences recited in SEQ ID NO: 45-88.
 6. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: (a) sequences having at least 75% identity to a sequence of SEQ ID NO: 45-88; (b) sequences having at least 90% identity to a sequence of SEQ ID NO: 45-88; and (c) sequences having at least 95% identity to a sequence of SEQ ID NO: 45-88, wherein the polypeptide has substantially the same functional properties as a polypeptide of SEQ ID NO: 45-88.
 7. An isolated polynucleotide that encodes a polypeptide of any one of claims 5 and
 6. 8. An isolated oligonucleotide probe or primer comprising at least 10 contiguous residues complementary to 10 contiguous residues of a nucleotide sequence recited in any one of claims 1-3.
 9. A kit comprising a plurality of oligonucleotide probes or primers of claim
 8. 10. A genetic construct comprising a polynucleotide of any one of claims 1-3.
 11. A transgenic cell comprising a genetic construct according to claim
 10. 12. A genetic construct comprising, in the 5′-3′ direction: (a) a gene promoter sequence; (b) a polynucleotide sequence comprising at least one of the following: (1) a polynucleotide coding for at least a functional portion of a polypeptide of any one of claims 5 and 6; and (2) a polynucleotide comprising a non-coding region of a polynucleotide of any one of claims 1-3; and (c) a gene termination sequence.
 13. The genetic construct of claim 12, wherein the polynucleotide sequence is in a sense orientation.
 14. The genetic construct of claim 12, wherein the polynucleotide is in an anti-sense orientation.
 15. A transgenic plant cell comprising a genetic construct of claim
 12. 16. A plant comprising a transgenic plant cell according to claim 12, or fruit or seeds or progeny thereof.
 17. The plant of claim 16, wherein the plant is selected from the group consisting of Festuca arundinacea and Lolium perenne species.
 18. A method for modulating at least one of the fructan composition, cellulose composition, starch composition and tannin composition of a plant, comprising modulating the activity of a polypeptide of any one of claims 5 and 6 in the plant.
 19. A method for modulating at least one of the fructan composition, cellulose composition, starch composition and tannin composition of a plant, comprising modulating the activity of a polynucleotide of any one of claims 1-3 in the plant.
 20. The method of claim 19, comprising stably incorporating into the genome of the plant a polynucleotide of any one of claims 1-3.
 21. The method of claim 19, comprising stably incorporating into the genome of the plant a genetic construct of claim
 12. 22. A method for producing a plant having at least one of altered fructan composition, altered cellulose composition, altered starch composition and altered tannin composition, comprising: (a) transforming a plant cell with a genetic construct of claim 12 to provide a transgenic cell; and (b) cultivating the transgenic cell under conditions conducive to regeneration and mature plant growth.
 23. A method for modifying the activity of a polypeptide involved in a fructan, cellulose, starch or tannin biosynthetic pathway in a plant, comprising modulating the activity of a polynucleotide of any one of claims 1-3 in the plant.
 24. The method of claim 23, comprising stably incorporating into the genome of the plant a genetic construct of claim
 12. 25. A method for modifying the activity of a polypeptide involved in a fructan, cellulose, starch or tannin biosynthetic pathway in a plant, comprising introducing into cells of the plant double stranded RNA corresponding to a polynucleotide of any one of claims 1-3, thereby inhibiting expression of a polypeptide encoded by the polynucleotide. 